The problems on identification
of Staphylococcus aureus isolates
from cheese samples were investigated by phenotypic and genotypic tests in this
study. Among 207 Staphylococcus spp. isolated from 31 cheese samples, 23
isolates that were Gram positive, catalase and slide coagulase positive, with 1
isolate that was latex agglutination test negative showed different phenotypic
properties. Polymerase chain reaction (PCR) and quantitative PCR (qPCR)
analyses showed that DNase test and target genes (nuc, coa) regarded as
gold standard regions for S. aureus
were not found to be unique for identification of S. aureus. The toxin genes (SEA-SEE) were not detected by PCR.
Antibiotic resistance profiles of S.
aureus isolates demonstrated that two isolates were resistant to penicillin
G. This study showed that the unique phenotypic and genotypic test was not
adequate for identification of S. aureus
isolates. There was no correlation between the presence of the nuc gene and toxin genes. The presence
of nuc gene which was used for
detection of S. aureus was also found
to be present in other Staphylococcus
isolates. As a conclusion, the results revealed that biochemical tests could
lead to false positive results for identification of S. aureus. The presence of nuc
gene is not correlated with the presence of toxin genes.
Staphylococcus aureus, PCR, Identification, Antibiotic resistance
Primary Language | English |
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Subjects | Food Science and Technology |
Journal Section | Research Articles |
Authors |
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Publication Date | July 1, 2019 |
Submission Date | October 12, 2018 |
Acceptance Date | November 29, 2018 |
Published in Issue | Year 2019Volume: 5 Issue: 3 |
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