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EXTRACTION, CHARACTERIZATION AND ANTIMICROBIAL ACTIVITY OF HYDROXYAPATITE FROM SEABASS AND SEABREAM SCALE

Year 2017, Volume 3, Issue 3, 90 - 96, 21.05.2017
https://doi.org/10.3153/JFHS17012

Abstract

The present study investigates the characterization of hydroxyapatite (HAp) extracted from seabass and seabream scales as by-product. Fish scales obtained from a seafood processing company were used to extract natural HAp powder.  HAp powder was extracted by alkaline heat treatment of fish scales and the synthesized HAp (FS-HAp) was extensively characterized with Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and X-ray diffraction (XRD) analysis. Calcium to phosphate ratio of the HAp was confirmed by inductively coupled plasma (ICP) and elemental analysis of HAp were also carried out using energy dispersive x-ray spectroscopy (EDS). The results of the characterization analysis were compared with commercial hydroxyapatite standard (CHAp) and it was clearly confirmed that the extracted FS-HAp exactly showed CHAp characteristics physicochemically which is used as biomaterial. However, well diffusion assay revealed out that synthesized hydroxyapatite showed no activity against C. albicans, S. aureus and E. coli. It was concluded that, instead of synthetic apatite, extracted FS-HAp presents a potential promising biomaterial as the raw materials are by-product which economically cheap and sustainable substances.

References

  • Alt, V., Bechert, T., Steinrücke, P., Wagener, M., Seidel, P., Dingeldein, E., Alt, V., Bechert, T., Steinrücke, P., Wagener, M., Seidel, P., Dingeldein, E., Domann, E. & Schnettler, R. (2004). In vitro testing of antimicrobial activity of bone cement. Antimicrobial agents and chemotherapy, 48(11), 4084-4088. Bardhan, R., Mahata, S. & Mondal, B. (2011). Processing of natural resourced hydroxyapatite from eggshell waste by wet precipitation method. Advances in Applied Ceramics, 110(2), 80-86. FAO, Globefish, (2015). Food and Agriculture Organization (FAO), Analysis and information on world fish trade, European Seabass and Gilthead seabream - March 2015, GLOBEFISH Market Reports, (http://www.fao.org/in-action/globefish/market-reports/resource detail/en/c/338048/) Gómez-Guillén, M.C., Giménez, B., López-Caballero, M.A. & Montero, M.P. (2011). Functional and bioactive properties of collagen and gelatin from alternative sources: A review. Food Hydrocolloids, 25(8), 1813-1827. Gumisiriza, R., Mshandete, A.M., Rubindamayugi, M.S.T., Kansiime, F. & Kivaisi, A. K. (2009). Nile perch fish processing waste along Lake Victoria in East Africa: Auditing and characterization. African Journal of Environmental Science and Technology, 3(1), 013-020. Huang, Y.C., Hsiao, P.C. & Chai, H.J. (2011). Hydroxyapatite extracted from fish scale: Effects on MG63 osteoblast-like cells. Ceramics International, 37(6), 1825-1831. Jensen, S.S., Aaboe, M., Pinholt, E.M., Hjoerting-Hansen, E., Melsen, F. & Ruyter, I.E. (1996). Tissue reaction and material characteristics of four bone substitutes. International Journal of Oral and Maxillofacial Implants, 11(1), 55-66. Kongsri, S., Janpradit, K., Buapa, K., Techawongstien, S. & Chanthai, S. (2013). Nanocrystalline hydroxyapatite from fish scale waste: Preparation, characterization and application for selenium adsorption in aqueous solution.Chemical engineering journal, 215, 522-532. Mondal, S., Mahata, S., Kundu, S. & Mondal, B. (2010). Processing of natural resourced hydroxyapatite ceramics from fish scale. Advances in Applied Ceramics, 109(4), 234-239. Mostafa, N.Y. (2005). Characterization, thermal stability and sintering of hydroxyapatite powders prepared by different routes. Materials Chemistry and Physics, 94(2), 333-341. Muhammad, N., Gao, Y., Iqbal, F., Ahmad, P., Ge, R., Nishan, U., Muhammad, N., Gao, Y., Iqbal, F., Ahmad, P., Ge, R., Nishan, U., Rahim, A., Gonfae, G. & Ullah, Z. (2016). Extraction of biocompatible hydroxyapatite from fish scales using novel approach of ionic liquid pretreatment. Separation and Purification Technology, 161, 129-135. National Committee for Clinical Laboratory Standards (NCCLS) (1993). ‘Approval standard M7-A3, Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically’, Villanova, PA, 1993. Ozawa, M. & Kanahara, S. (2005). Removal of aqueous lead by fish-bone waste hydroxyapatite powder. Journal of Materials Science, 40(4), 1037-1038. Ozawa, M. & Suzuki, S. (2002). Microstructural development of natural hydroxyapatite originated from fish-bone waste through heat treatment. Journal of the American Ceramic Society, 85(5), 1315-1317. Panda, N. N., Pramanik, K. & Sukla, L.B. (2014). Extraction and characterization of biocompatible hydroxyapatite from fresh water fish scales for tissue engineering scaffold. Bioprocess and Biosystems Engineering, 37(3), 433-440. Prabakaran, K., Balamurugan, A. & Rajeswari, S. (2005). Development of calcium phosphate based apatite from hen’s eggshell. Bulletin of Materials Science, 28(2), 115-119. Pon-On, W., Suntornsaratoon, P., Charoenphandhu, N., Thongbunchoo, J., Krishnamra, N. & Tang, I.M. (2016). Hydroxyapatite from fish scale for potential use as bone scaffold or regenerative material. Materials Science and Engineering: C, 62, 183-189. Sanosh, K.P., Chu, M.C., Balakrishnan, A., Lee, Y.J., Kim, T.N. & Cho, S.J. (2009). Synthesis of nano hydroxyapatite powder that simulate teeth particle morphology and composition. Current Applied Physics, 9(6), 1459-1462. Sofronia, A.M., Baies, R., Anghel, E.M., Marinescu, C.A. & Tanasescu, S. (2014). Thermal and structural characterization of synthetic and natural nanocrystalline hydroxyapatite. Materials Science and Engineering: C, 43, 153-163. Stoch, A., Jastrzębski, W., Brożek, A., Stoch, J., Szaraniec, J., Trybalska, B., & Kmita, G. (2000). FTIR absorption–reflection study of biomimetic growth of phosphates on titanium implants. Journal of Molecular Structure, 555(1), 375-382. Turkish Statistical Institute (TUIK), 2015, Fishery Statistics 2015. https://www.tarim.gov.tr/sgb/Belgeler/SagMenuVeriler/BSGM.pdf (accesed 02.05.2017) Venkatesan, J., Lowe, B., Manivasagan, P., Kang, K. H., Chalisserry, E. P., Anil, S., ... & Kim, S. K. (2015). Isolation and Characterization of Nano-Hydroxyapatite from Salmon Fish Bone. Materials, 8(8), 5426-5439. Zainon, I., Alwi, N.M., Abidin, M.Z., Haniza, H. M.Z., Ahmad, M.S. & Ramli, A. (2012). Physicochemical properties of hydroxyapatite extracted from fish scales. Advanced Materials Research, 545, 235-239.

Year 2017, Volume 3, Issue 3, 90 - 96, 21.05.2017
https://doi.org/10.3153/JFHS17012

Abstract

References

  • Alt, V., Bechert, T., Steinrücke, P., Wagener, M., Seidel, P., Dingeldein, E., Alt, V., Bechert, T., Steinrücke, P., Wagener, M., Seidel, P., Dingeldein, E., Domann, E. & Schnettler, R. (2004). In vitro testing of antimicrobial activity of bone cement. Antimicrobial agents and chemotherapy, 48(11), 4084-4088. Bardhan, R., Mahata, S. & Mondal, B. (2011). Processing of natural resourced hydroxyapatite from eggshell waste by wet precipitation method. Advances in Applied Ceramics, 110(2), 80-86. FAO, Globefish, (2015). Food and Agriculture Organization (FAO), Analysis and information on world fish trade, European Seabass and Gilthead seabream - March 2015, GLOBEFISH Market Reports, (http://www.fao.org/in-action/globefish/market-reports/resource detail/en/c/338048/) Gómez-Guillén, M.C., Giménez, B., López-Caballero, M.A. & Montero, M.P. (2011). Functional and bioactive properties of collagen and gelatin from alternative sources: A review. Food Hydrocolloids, 25(8), 1813-1827. Gumisiriza, R., Mshandete, A.M., Rubindamayugi, M.S.T., Kansiime, F. & Kivaisi, A. K. (2009). Nile perch fish processing waste along Lake Victoria in East Africa: Auditing and characterization. African Journal of Environmental Science and Technology, 3(1), 013-020. Huang, Y.C., Hsiao, P.C. & Chai, H.J. (2011). Hydroxyapatite extracted from fish scale: Effects on MG63 osteoblast-like cells. Ceramics International, 37(6), 1825-1831. Jensen, S.S., Aaboe, M., Pinholt, E.M., Hjoerting-Hansen, E., Melsen, F. & Ruyter, I.E. (1996). Tissue reaction and material characteristics of four bone substitutes. International Journal of Oral and Maxillofacial Implants, 11(1), 55-66. Kongsri, S., Janpradit, K., Buapa, K., Techawongstien, S. & Chanthai, S. (2013). Nanocrystalline hydroxyapatite from fish scale waste: Preparation, characterization and application for selenium adsorption in aqueous solution.Chemical engineering journal, 215, 522-532. Mondal, S., Mahata, S., Kundu, S. & Mondal, B. (2010). Processing of natural resourced hydroxyapatite ceramics from fish scale. Advances in Applied Ceramics, 109(4), 234-239. Mostafa, N.Y. (2005). Characterization, thermal stability and sintering of hydroxyapatite powders prepared by different routes. Materials Chemistry and Physics, 94(2), 333-341. Muhammad, N., Gao, Y., Iqbal, F., Ahmad, P., Ge, R., Nishan, U., Muhammad, N., Gao, Y., Iqbal, F., Ahmad, P., Ge, R., Nishan, U., Rahim, A., Gonfae, G. & Ullah, Z. (2016). Extraction of biocompatible hydroxyapatite from fish scales using novel approach of ionic liquid pretreatment. Separation and Purification Technology, 161, 129-135. National Committee for Clinical Laboratory Standards (NCCLS) (1993). ‘Approval standard M7-A3, Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically’, Villanova, PA, 1993. Ozawa, M. & Kanahara, S. (2005). Removal of aqueous lead by fish-bone waste hydroxyapatite powder. Journal of Materials Science, 40(4), 1037-1038. Ozawa, M. & Suzuki, S. (2002). Microstructural development of natural hydroxyapatite originated from fish-bone waste through heat treatment. Journal of the American Ceramic Society, 85(5), 1315-1317. Panda, N. N., Pramanik, K. & Sukla, L.B. (2014). Extraction and characterization of biocompatible hydroxyapatite from fresh water fish scales for tissue engineering scaffold. Bioprocess and Biosystems Engineering, 37(3), 433-440. Prabakaran, K., Balamurugan, A. & Rajeswari, S. (2005). Development of calcium phosphate based apatite from hen’s eggshell. Bulletin of Materials Science, 28(2), 115-119. Pon-On, W., Suntornsaratoon, P., Charoenphandhu, N., Thongbunchoo, J., Krishnamra, N. & Tang, I.M. (2016). Hydroxyapatite from fish scale for potential use as bone scaffold or regenerative material. Materials Science and Engineering: C, 62, 183-189. Sanosh, K.P., Chu, M.C., Balakrishnan, A., Lee, Y.J., Kim, T.N. & Cho, S.J. (2009). Synthesis of nano hydroxyapatite powder that simulate teeth particle morphology and composition. Current Applied Physics, 9(6), 1459-1462. Sofronia, A.M., Baies, R., Anghel, E.M., Marinescu, C.A. & Tanasescu, S. (2014). Thermal and structural characterization of synthetic and natural nanocrystalline hydroxyapatite. Materials Science and Engineering: C, 43, 153-163. Stoch, A., Jastrzębski, W., Brożek, A., Stoch, J., Szaraniec, J., Trybalska, B., & Kmita, G. (2000). FTIR absorption–reflection study of biomimetic growth of phosphates on titanium implants. Journal of Molecular Structure, 555(1), 375-382. Turkish Statistical Institute (TUIK), 2015, Fishery Statistics 2015. https://www.tarim.gov.tr/sgb/Belgeler/SagMenuVeriler/BSGM.pdf (accesed 02.05.2017) Venkatesan, J., Lowe, B., Manivasagan, P., Kang, K. H., Chalisserry, E. P., Anil, S., ... & Kim, S. K. (2015). Isolation and Characterization of Nano-Hydroxyapatite from Salmon Fish Bone. Materials, 8(8), 5426-5439. Zainon, I., Alwi, N.M., Abidin, M.Z., Haniza, H. M.Z., Ahmad, M.S. & Ramli, A. (2012). Physicochemical properties of hydroxyapatite extracted from fish scales. Advanced Materials Research, 545, 235-239.

Details

Subjects Food Science and Technology, Biology
Journal Section Articles
Authors

Yunus Alparslan
Muğla Sıtkı Koçman University, Faculty of Fisheries
0000-0002-8833-996X
Türkiye


Tuba Baygar
Mugla Sitki Kocman University, Research Laboratories Center
0000-0002-1238-3227
Türkiye


Taçnur Baygar
Muğla Sıtkı Koçman University, Faculty of Fisheries
Türkiye

Publication Date May 21, 2017
Application Date February 14, 2017
Acceptance Date April 18, 2017
Published in Issue Year 2017, Volume 3, Issue 3

Cite

Bibtex @research article { jfhs315183, journal = {Food and Health}, issn = {}, eissn = {2602-2834}, address = {Vidin Caddesi No:28 D:4 Kocamustafapaşa 34107 Fatih İstanbul}, publisher = {Özkan ÖZDEN}, year = {2017}, volume = {3}, pages = {90 - 96}, doi = {10.3153/JFHS17012}, title = {EXTRACTION, CHARACTERIZATION AND ANTIMICROBIAL ACTIVITY OF HYDROXYAPATITE FROM SEABASS AND SEABREAM SCALE}, key = {cite}, author = {Alparslan, Yunus and Baygar, Tuba and Baygar, Taçnur} }
APA Alparslan, Y. , Baygar, T. & Baygar, T. (2017). EXTRACTION, CHARACTERIZATION AND ANTIMICROBIAL ACTIVITY OF HYDROXYAPATITE FROM SEABASS AND SEABREAM SCALE . Food and Health , 3 (3) , 90-96 . DOI: 10.3153/JFHS17012
MLA Alparslan, Y. , Baygar, T. , Baygar, T. "EXTRACTION, CHARACTERIZATION AND ANTIMICROBIAL ACTIVITY OF HYDROXYAPATITE FROM SEABASS AND SEABREAM SCALE" . Food and Health 3 (2017 ): 90-96 <http://jfhs.scientificwebjournals.com/en/pub/issue/28293/315183>
Chicago Alparslan, Y. , Baygar, T. , Baygar, T. "EXTRACTION, CHARACTERIZATION AND ANTIMICROBIAL ACTIVITY OF HYDROXYAPATITE FROM SEABASS AND SEABREAM SCALE". Food and Health 3 (2017 ): 90-96
RIS TY - JOUR T1 - EXTRACTION, CHARACTERIZATION AND ANTIMICROBIAL ACTIVITY OF HYDROXYAPATITE FROM SEABASS AND SEABREAM SCALE AU - Yunus Alparslan , Tuba Baygar , Taçnur Baygar Y1 - 2017 PY - 2017 N1 - doi: 10.3153/JFHS17012 DO - 10.3153/JFHS17012 T2 - Food and Health JF - Journal JO - JOR SP - 90 EP - 96 VL - 3 IS - 3 SN - -2602-2834 M3 - doi: 10.3153/JFHS17012 UR - https://doi.org/10.3153/JFHS17012 Y2 - 2017 ER -
EndNote %0 Food and Health EXTRACTION, CHARACTERIZATION AND ANTIMICROBIAL ACTIVITY OF HYDROXYAPATITE FROM SEABASS AND SEABREAM SCALE %A Yunus Alparslan , Tuba Baygar , Taçnur Baygar %T EXTRACTION, CHARACTERIZATION AND ANTIMICROBIAL ACTIVITY OF HYDROXYAPATITE FROM SEABASS AND SEABREAM SCALE %D 2017 %J Food and Health %P -2602-2834 %V 3 %N 3 %R doi: 10.3153/JFHS17012 %U 10.3153/JFHS17012
ISNAD Alparslan, Yunus , Baygar, Tuba , Baygar, Taçnur . "EXTRACTION, CHARACTERIZATION AND ANTIMICROBIAL ACTIVITY OF HYDROXYAPATITE FROM SEABASS AND SEABREAM SCALE". Food and Health 3 / 3 (May 2017): 90-96 . https://doi.org/10.3153/JFHS17012
AMA Alparslan Y. , Baygar T. , Baygar T. EXTRACTION, CHARACTERIZATION AND ANTIMICROBIAL ACTIVITY OF HYDROXYAPATITE FROM SEABASS AND SEABREAM SCALE. Food Health. 2017; 3(3): 90-96.
Vancouver Alparslan Y. , Baygar T. , Baygar T. EXTRACTION, CHARACTERIZATION AND ANTIMICROBIAL ACTIVITY OF HYDROXYAPATITE FROM SEABASS AND SEABREAM SCALE. Food and Health. 2017; 3(3): 90-96.
IEEE Y. Alparslan , T. Baygar and T. Baygar , "EXTRACTION, CHARACTERIZATION AND ANTIMICROBIAL ACTIVITY OF HYDROXYAPATITE FROM SEABASS AND SEABREAM SCALE", Food and Health, vol. 3, no. 3, pp. 90-96, May. 2017, doi:10.3153/JFHS17012

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