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Nigella sativa OIL COULD INDUCE OSTEOGENIC DIFFERENTIATION OF DENTAL PULP MESENCHYMAL STEM CELLS: CLINICAL NUTRITION FOR DENTISTRY

Yıl 2018, Cilt 4, Sayı 1, 19 - 24, 01.01.2018
https://doi.org/10.3153/JFHS18003

Öz

A natural agent that, promotes osteogenic differentiation of dental pulp mesenchymal stem cells (DP-MSCs) could achieve success in regeneration during healing and may also prevent bone resorption and improve regeneration. We aimed to demonstrate that a Nigella sativa oil could induce differentiation of DP-MSCs. DP-MSCs were isolated from 3. molars and identified with flow cytometer. Osteogenic differentiation was conducted, and calcium granules were showed by typical Alizarin Red dying and calcium concentration were determined by DICA 500. Identification results showed that the cells are mesenchymal stem cells. Alizarin Red dying signed the calcium granules and DICA 500 showed that calcium concentrations were higher than the Control Group. This preliminary study shows for the first time the inductive potential of N. sativa oil for osteogenic differentiation in DP-MSCs. The mechanism should be investigated in further studies. 

Kaynakça

  • Abd-Awn, B., Al-Dhaher, Z. & Al-Dafaai, R. (2012). The effect of black seed oil extracts on mutans streptococci in comparison to chlorhexidine gluconate (in vitro). Journal of Baghdad College of Dentistry, 24, 126-131.
  • Ali, B.H. & Blunden, G. (2003). Pharmacological and toxicological properties of Nigella sativa. Phytother. Res. 17, 299-305.
  • Kirui, P.K., Cameron, J., Benghuzzi, H.A., Tucci, M., Patel, R., Adah, F. & Russell, G. (2004). Effects of sustained delivery of thymoqiunone on bone healing of male rats. Biomedical Science Instrumentation, 40, 111-116.
  • D'aquino, R., Grazıano, A., Sampaolesi, M., Laino, G., Pırozzı, G., De Rosa, A. & Papaccio, G. (2007). Human postnatal dental pulp cells co-differentiate into osteoblasts and endotheliocytes: a pivotal synergy leading to adult bone tissue formation. Cell Death & Differentiation. 14, 1162–1171.
  • Egermann, M., Goldhahn, J. & Schneider, E. (2005). Animal models for fracture treatment in osteoporosis. Osteoporos International, 16(suppl2), 129-138.
  • Gali-Muhtasib, H., Roessner, A. & Schneider-Stock, R. (2005). Thymoquinone: a promising anti-cancer drug from natural sources. The International Journal of Biochemistry & Cell Biology, 38, 1249-1253.
  • Gronthos, S., Mankani, M., Brahim, J., Robey, P.G. & Shi, S. (2000). Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proceedings of the Natiıonal Academy of Science, 97, 13625-13630.
  • Harzallah, H., Kouidhi, B., Flamini, G., Bakhrouf, A. & Mahjoub, T. (2011). Chemical composition, antimicrobial potential against cariogenic bacteria and cytotoxic activity of Tunisian Nigella sativa essential oil and thymoquinone. Food Chemistry, 129,1469-1474.
  • Hoiruchi, K., Amizuka, N., Takeshita, S., Takamatsu, H., Katsuura, M., Ozawa, H., Toyama, Y., Bonewald, L.F. & Kudo, A. (1999). Identification and chracterization of a novel protein, perostin, with restricted expression to periosteum and periodontal ligament and increased expression by transforming growth factor beta. Journal of Bone & Mineral Research, 14,1239-1249.
  • Kaleem, M., Kirmani, D., Asif, M., Ahmed, Q., Bano, B. (2006). Biochemical effects of Nigella sativa L. seeds in diabetic rats. Indian Journal of Experimental Biology, 44, 745-748.
  • Kanter, M., Coşkun, O. & Uysal, H. (2006). The antioxidative and antihistaminic effect of Nigella sativa and its major constituent, thymoquinone on ethanol-induced gastric mucosal damage. Archves of Toxicology, 80, 217-224.
  • Mabrouk, G.M., Moselhy, S.S., Zohny, S.F., Ali, E.M., Helal, T.E., Amin, A.A. & Khalifa, A.A. (2002). Inhibition of methylnitrosourea (MNU) induced oxidative stress and carcinogenesis by orally administered bee honey and Nigella grains in Sprague Dawley rats. Journal of Experimental and Clinical Cancer Research, 21, 341-346.
  • Pisciota, A., Carnevale G., Meloni, S., Ricio, M., Biasi, S.D., Gibellini, L., Ferrari, A., Bruzzesi, G. & de Pol, A. (2015). Human dental pulp stem cells (hDPSCs): isolation, enrichment and comparative differentiation of two sub-populations. BMC Developmental Biology, 15, 14-16
  • Wirries, A., Schubert, A.K., Zimmermann, R., Jabari, S., Ruchholtz, S. & El-Najjar, N. (2013). Thymoquinone accelerates osteoblast differentiation and activates bone morphogenetic protein-2 and ERK pathway. International Immunopharmacology, 15(2), 381-386.
  • Ozdemir, H., Kara, M.I., Erciyas, K., Ozer, H. & Ay, S. (2012). Preventive effects of thymoquinone in a rat periodontitis model: a morphometric and histopathological study. Journal of Periodontal Research, 47, 74-80.
  • Pittenger, M.F., Mackay, A.M., Beck, S. (1999). Multilineage potential of adult human mesenchymal stem cells. Science, 284, 1168-1170.
  • Shaker, A. & Al-Wafi, H. (2014). Benefits of thymoquinone, a Nigella sativa extract in preventing dental caries initiation and improving gingival health. ProQuest LLC, 72.

Yıl 2018, Cilt 4, Sayı 1, 19 - 24, 01.01.2018
https://doi.org/10.3153/JFHS18003

Öz

Kaynakça

  • Abd-Awn, B., Al-Dhaher, Z. & Al-Dafaai, R. (2012). The effect of black seed oil extracts on mutans streptococci in comparison to chlorhexidine gluconate (in vitro). Journal of Baghdad College of Dentistry, 24, 126-131.
  • Ali, B.H. & Blunden, G. (2003). Pharmacological and toxicological properties of Nigella sativa. Phytother. Res. 17, 299-305.
  • Kirui, P.K., Cameron, J., Benghuzzi, H.A., Tucci, M., Patel, R., Adah, F. & Russell, G. (2004). Effects of sustained delivery of thymoqiunone on bone healing of male rats. Biomedical Science Instrumentation, 40, 111-116.
  • D'aquino, R., Grazıano, A., Sampaolesi, M., Laino, G., Pırozzı, G., De Rosa, A. & Papaccio, G. (2007). Human postnatal dental pulp cells co-differentiate into osteoblasts and endotheliocytes: a pivotal synergy leading to adult bone tissue formation. Cell Death & Differentiation. 14, 1162–1171.
  • Egermann, M., Goldhahn, J. & Schneider, E. (2005). Animal models for fracture treatment in osteoporosis. Osteoporos International, 16(suppl2), 129-138.
  • Gali-Muhtasib, H., Roessner, A. & Schneider-Stock, R. (2005). Thymoquinone: a promising anti-cancer drug from natural sources. The International Journal of Biochemistry & Cell Biology, 38, 1249-1253.
  • Gronthos, S., Mankani, M., Brahim, J., Robey, P.G. & Shi, S. (2000). Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proceedings of the Natiıonal Academy of Science, 97, 13625-13630.
  • Harzallah, H., Kouidhi, B., Flamini, G., Bakhrouf, A. & Mahjoub, T. (2011). Chemical composition, antimicrobial potential against cariogenic bacteria and cytotoxic activity of Tunisian Nigella sativa essential oil and thymoquinone. Food Chemistry, 129,1469-1474.
  • Hoiruchi, K., Amizuka, N., Takeshita, S., Takamatsu, H., Katsuura, M., Ozawa, H., Toyama, Y., Bonewald, L.F. & Kudo, A. (1999). Identification and chracterization of a novel protein, perostin, with restricted expression to periosteum and periodontal ligament and increased expression by transforming growth factor beta. Journal of Bone & Mineral Research, 14,1239-1249.
  • Kaleem, M., Kirmani, D., Asif, M., Ahmed, Q., Bano, B. (2006). Biochemical effects of Nigella sativa L. seeds in diabetic rats. Indian Journal of Experimental Biology, 44, 745-748.
  • Kanter, M., Coşkun, O. & Uysal, H. (2006). The antioxidative and antihistaminic effect of Nigella sativa and its major constituent, thymoquinone on ethanol-induced gastric mucosal damage. Archves of Toxicology, 80, 217-224.
  • Mabrouk, G.M., Moselhy, S.S., Zohny, S.F., Ali, E.M., Helal, T.E., Amin, A.A. & Khalifa, A.A. (2002). Inhibition of methylnitrosourea (MNU) induced oxidative stress and carcinogenesis by orally administered bee honey and Nigella grains in Sprague Dawley rats. Journal of Experimental and Clinical Cancer Research, 21, 341-346.
  • Pisciota, A., Carnevale G., Meloni, S., Ricio, M., Biasi, S.D., Gibellini, L., Ferrari, A., Bruzzesi, G. & de Pol, A. (2015). Human dental pulp stem cells (hDPSCs): isolation, enrichment and comparative differentiation of two sub-populations. BMC Developmental Biology, 15, 14-16
  • Wirries, A., Schubert, A.K., Zimmermann, R., Jabari, S., Ruchholtz, S. & El-Najjar, N. (2013). Thymoquinone accelerates osteoblast differentiation and activates bone morphogenetic protein-2 and ERK pathway. International Immunopharmacology, 15(2), 381-386.
  • Ozdemir, H., Kara, M.I., Erciyas, K., Ozer, H. & Ay, S. (2012). Preventive effects of thymoquinone in a rat periodontitis model: a morphometric and histopathological study. Journal of Periodontal Research, 47, 74-80.
  • Pittenger, M.F., Mackay, A.M., Beck, S. (1999). Multilineage potential of adult human mesenchymal stem cells. Science, 284, 1168-1170.
  • Shaker, A. & Al-Wafi, H. (2014). Benefits of thymoquinone, a Nigella sativa extract in preventing dental caries initiation and improving gingival health. ProQuest LLC, 72.

Ayrıntılar

Konular Tıp
Bölüm Articles
Yazarlar

Ayşegül MENDİ
Gazi University, Faculty of Dentistry, Department of Medical Microbiology, Emek Ankara
0000-0001-6705-3770
Türkiye

Yayımlanma Tarihi 1 Ocak 2018
Başvuru Tarihi 20 Mart 2017
Kabul Tarihi 13 Ağustos 2017
Yayınlandığı Sayı Yıl 2018, Cilt 4, Sayı 1

Kaynak Göster

Bibtex @araştırma makalesi { jfhs349685, journal = {Food and Health}, issn = {}, eissn = {2602-2834}, address = {Vidin Caddesi No:28 D:4 Kocamustafapaşa 34107 Fatih İstanbul}, publisher = {Özkan ÖZDEN}, year = {2018}, volume = {4}, pages = {19 - 24}, doi = {10.3153/JFHS18003}, title = {Nigella sativa OIL COULD INDUCE OSTEOGENIC DIFFERENTIATION OF DENTAL PULP MESENCHYMAL STEM CELLS: CLINICAL NUTRITION FOR DENTISTRY}, key = {cite}, author = {Mendi, Ayşegül} }
APA Mendi, A. (2018). Nigella sativa OIL COULD INDUCE OSTEOGENIC DIFFERENTIATION OF DENTAL PULP MESENCHYMAL STEM CELLS: CLINICAL NUTRITION FOR DENTISTRY . Food and Health , 4 (1) , 19-24 . DOI: 10.3153/JFHS18003
MLA Mendi, A. "Nigella sativa OIL COULD INDUCE OSTEOGENIC DIFFERENTIATION OF DENTAL PULP MESENCHYMAL STEM CELLS: CLINICAL NUTRITION FOR DENTISTRY" . Food and Health 4 (2018 ): 19-24 <http://jfhs.scientificwebjournals.com/tr/pub/issue/31825/349685>
Chicago Mendi, A. "Nigella sativa OIL COULD INDUCE OSTEOGENIC DIFFERENTIATION OF DENTAL PULP MESENCHYMAL STEM CELLS: CLINICAL NUTRITION FOR DENTISTRY". Food and Health 4 (2018 ): 19-24
RIS TY - JOUR T1 - Nigella sativa OIL COULD INDUCE OSTEOGENIC DIFFERENTIATION OF DENTAL PULP MESENCHYMAL STEM CELLS: CLINICAL NUTRITION FOR DENTISTRY AU - Ayşegül Mendi Y1 - 2018 PY - 2018 N1 - doi: 10.3153/JFHS18003 DO - 10.3153/JFHS18003 T2 - Food and Health JF - Journal JO - JOR SP - 19 EP - 24 VL - 4 IS - 1 SN - -2602-2834 M3 - doi: 10.3153/JFHS18003 UR - https://doi.org/10.3153/JFHS18003 Y2 - 2017 ER -
EndNote %0 Food and Health Nigella sativa OIL COULD INDUCE OSTEOGENIC DIFFERENTIATION OF DENTAL PULP MESENCHYMAL STEM CELLS: CLINICAL NUTRITION FOR DENTISTRY %A Ayşegül Mendi %T Nigella sativa OIL COULD INDUCE OSTEOGENIC DIFFERENTIATION OF DENTAL PULP MESENCHYMAL STEM CELLS: CLINICAL NUTRITION FOR DENTISTRY %D 2018 %J Food and Health %P -2602-2834 %V 4 %N 1 %R doi: 10.3153/JFHS18003 %U 10.3153/JFHS18003
ISNAD Mendi, Ayşegül . "Nigella sativa OIL COULD INDUCE OSTEOGENIC DIFFERENTIATION OF DENTAL PULP MESENCHYMAL STEM CELLS: CLINICAL NUTRITION FOR DENTISTRY". Food and Health 4 / 1 (Ocak 2018): 19-24 . https://doi.org/10.3153/JFHS18003
AMA Mendi A. Nigella sativa OIL COULD INDUCE OSTEOGENIC DIFFERENTIATION OF DENTAL PULP MESENCHYMAL STEM CELLS: CLINICAL NUTRITION FOR DENTISTRY. Food Health. 2018; 4(1): 19-24.
Vancouver Mendi A. Nigella sativa OIL COULD INDUCE OSTEOGENIC DIFFERENTIATION OF DENTAL PULP MESENCHYMAL STEM CELLS: CLINICAL NUTRITION FOR DENTISTRY. Food and Health. 2018; 4(1): 19-24.
IEEE A. Mendi , "Nigella sativa OIL COULD INDUCE OSTEOGENIC DIFFERENTIATION OF DENTAL PULP MESENCHYMAL STEM CELLS: CLINICAL NUTRITION FOR DENTISTRY", Food and Health, c. 4, sayı. 1, ss. 19-24, Oca. 2018, doi:10.3153/JFHS18003

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