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Investigation of cyto-genotoxic effects of a food sweetener Acesulfame potassium

Year 2022, Volume 8, Issue 4, 273 - 283, 01.10.2022
https://doi.org/10.3153/FH22025

Abstract

Acesulfame potassium (ACE-K) is an artificial sweetener widely used in many foods. This investigation assessed the cytotoxic effect of ACE-K using MTT assay in human hepatocellular carcinoma (HepG2) cell line and the genotoxic effect using chromosomal aberrations (CAs), micronucleus (MN), and comet assays in human lymphocytes. 7.5-240 μg/mL concentrations of ACE-K were applied to cells. ACE-K notably decreased the cell viability on HepG2 cells, especially at 120 and 240 μg/mL at 24 and 48 h. It also significantly reduced the mitotic index (MI) at 60, 120, and 240 μg/mL at both treatments (24 and 48 h) in human lymphocytes. The frequency of the CAs significantly increased at 60, 120, and 240 μg/mL for 48 h treatment compared to control. However, no difference was observed in the frequency of MN and nuclear division index (NDI) at all the treatments. ACE-K also induced comet tail length, tail intensity, and moment at 15 μg/mL in isolated human lymphocytes. Therefore, ACE-K showed a cytotoxic effect in HepG2 cells as well as human lymphocytes at higher concentrations. It also exhibits a mild genotoxic effect by increasing the frequency of CAs at long-term treatment and DNA damaging effect only at 15 μg/mL.

References

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Year 2022, Volume 8, Issue 4, 273 - 283, 01.10.2022
https://doi.org/10.3153/FH22025

Abstract

References

  • Ashok, I., Poornima, P.S., Wankhar, D., Ravindran, R., Sheeladevi, R. (2017). Oxidative stress evoked damages on rat sperm and attenuated antioxidant status on consumption of aspartame. International Journal of Impotence Research, 29(4), 164-170. https://doi.org/10.1038/ijir.2017.17
  • Bach, D-H., Zhang, W., Sood, A.K. (2019). Chromosomal Instability in Tumor Initiation and Development. Cancer Research, 79(16), 3995–4002. https://doi.org/10.1158/0008-5472.CAN-18-3235
  • Bandyopadhyay, A., Ghoshal, S., Mukherjee, A. (2008). Genotoxicity testing of low-calorie sweeteners: aspartame, acesulfame-K, and saccharin. Drug and Chemical Toxicology, 31(4), 447-457. https://doi.org/10.1080/01480540802390270
  • Belton, K., Schaefer, E., Guiney, P.D. (2020). A review of the environmental fate and effects of acesulfame‐potassium. Integrated Environmental Assessment and Management, 16(4), 421-437. https://doi.org/10.1002/ieam.4248
  • Cao, Y., Liu, H., Qin, N., Ren, X., Zhu, B., Xiaodong, X. (2020). Impact of food additives on the composition and function of gut microbiota: A review. Trends in Food Science & Technology, 99, 295-310. https://doi.org/10.1016/j.tifs.2020.03.006
  • Carocho, M., Morales, P., Ferreira, I.C. (2017). Sweeteners as food additives in the XXI century: A review of what is known, and what is to come. Food and Chemical Toxicology, 107, 302-317. https://doi.org/10.1016/j.fct.2017.06.046
  • Chappell, G.A., Wikoff, D.S., Doepker, C.L., Borghoff, S.J. (2020). Lack of potential carcinogenicity for acesulfame potassium–systematic evaluation and integration of mechanistic data into the totality of the evidence. Food and Chemical Toxicology, 141, 111375. https://doi.org/10.1016/j.fct.2020.111375
  • Chattopadhyay, S., Raychaudhuri, U., Chakraborty, R. (2014). Artificial sweeteners–a review. Journal of Food Science and Technology, 51(4), 611-621. https://doi.org/10.1007/s13197-011-0571-1
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  • De Koning, L., Malik, V.S., Rimm, E.B., Willett, W.C., Hu, F.B., (2011). Sugar-sweetened and artificially sweetened beverage consumption and risk of type 2 diabetes in men. The American Journal of Clinical Nutrition, 93, 1321-1327. https://doi.org/10.3945/ajcn.110.007922
  • Dong, G., Li, X., Han, G., Du, L., Li, M. (2020). Zebrafish neuro-behavioral profiles altered by acesulfame (ACE) within the range of “no observed effect concentrations (NOECs)”. Chemosphere, 243, 125431. https://doi.org/10.1016/j.chemosphere.2019.125431
  • Dusinska, M., Mariussen, E., Rundén Pran, E., Hudecova, A.M., Elje, E., Kazimirova, A., et al. (2019). In vitro approaches for assessing the genotoxicity of nanomaterials. Qunwei Zhang (ed.), Nanotoxicity: Methods and Protocols, Methods in Molecular Biology. Nature, 1894, 83-122. https://doi.org/10.1007/978-1-4939-8916-4_6
  • Erikel, E., Yuzbasioglu, D., Unal, F. (2020). Genotoxic and antigenotoxic potential of amygdalin on isolated human lymphocytes by the comet assay. Journal of Food Biochemistry, 44(10), e13436. https://doi.org/10.1111/jfbc.13436
  • Eroglu, Y., Eroglu, H.E., Ilbas, A.I. (2007). Gamma ray reduces mitotik index in embriyonic roots of Hordeum vulgare L. Advanced Biomedical Research, 1(2), 26-28.
  • Evans, H.J. (1984). Human peripheral blood lymphocytes for the analysis of chromosome aberrations in mutagentests in mutagen tests. In: B.J. Kilbey, M. Legator, W. Nichols, C. Ramel, (eds). Handbook of mutagenicity test procedures. Amsterdam: Elsevier Science Publishers, 405-427. https://doi.org/10.1016/B978-0-444-80519-5.50023-7
  • Fenech M. (2000). The in vitro micronucleus technique. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 455(1-2), 81-95. https://doi.org/10.1016/S0027-5107(00)00065-8
  • Fenech, M., Knasmueller, S., Bolognesi, C., Holland, N., Bonassi, S., Kirsch-Volders, M. (2020). Micronuclei as biomarkers of DNA damage, aneuploidy, inducers of chromosomal hypermutation and as sources of pro-inflammatory DNA in humans. Mutation Research/Reviews in Mutation Research, 786, 108342. https://doi.org/10.1016/j.mrrev.2020.108342
  • Fındıklı, Z., Türkoglu, S., (2014). Determination of the effects of some artificial sweeteners on human peripheral lymphocytes using the comet assay. Journal of Toxicology and Environmental Health Sciences, 6(8), 147-153. https://doi.org/10.5897/JTEHS2014.0313
  • Fowler, S.P., Williams, K., Resendez, R.G., Hunt, K., Hazuda, H.P., Stern, M.P. (2008). Fueling the obesity epidemic? Artificially sweetened beverage use and longterm weight gain. Obesity, 16(8), 1894-1900. https://doi.org/10.1038/oby.2008.284
  • Fowler, S.P.G., (2016). Low-calorie sweetener use and energy balance: Results from experimental studies in animals, and large-scale prospective studies in humans. Physiology Behavior, 164, 517-523. https://doi.org/10.1016/j.physbeh.2016.04.047
  • Fung, T.T., Malik, V., Rexrode, K.M., Manson, J.E., Willett, W.C., Hu, F.B. (2009). Sweetened beverage consumption and risk of coronary heart disease in women. The American Journal of Clinical Nutrition, 89(4), 1037-1042. https://doi.org/10.3945/ajcn.2008.27140
  • Heredia-García, G., Gómez Oliván, L.M., Orozco Hernández, J.M., Luja Mondragón, M., Islas Flores, H., Juan Reyes, N. S., Galar-Martínez M., García-Medina, S., Dublán-García, O. (2019). Alterations to DNA, apoptosis and oxidative damage induced by sucralose in blood cells of Cyprinus carpio. Science of the Total Environment, 692, 411-421. https://doi.org/10.1016/j.scitotenv.2019.07.165
  • Hernández-Pérez, A.F., Jofre, F.M., Queiroz, S., de Arruda, P.V., Chandel, A.K., Graç, M.D., Almeida, F. (2020). Chapter 9: Biotechnological production of sweeteners. Verma, M., Chandel, A., (Eds). Biotechnological Production of Bioactive Compounds, Publisher: Elsevier, 261-292. https://doi.org/10.1016/B978-0-444-64323-0.00009-6
  • Ibi, D., Suzuki, F., Hiramatsu, M. (2018). Effect of AceK (acesulfame potassium) on brain function under dietary restriction in mice. Physiology & Behavior, 188, 291-297. https://doi.org/10.1016/j.physbeh.2018.02.024
  • Jain, A.K., Sorbhoy, R.K. (1988). Cytogenetical studies on the effects of some chlorinated pesticides. III. Concluding remarks. Cytologia, 53(3), 427-436. https://doi.org/10.1508/cytologia.53.427
  • Jeffrey, A.M., Williams, G.M. (2000). Lack of DNA-damaging activity of five non-nutritive sweeteners in the rat hepatocyte/DNA repair assay. Food and Chemical Toxicology, 38(4), 335-338. https://doi.org/10.1016/S0278-6915(99)00163-5
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Details

Primary Language English
Subjects Biology
Journal Section Research Articles
Authors

Sevcan MAMUR> (Primary Author)
Gazi University, Life Sciences Application and Research Center
0000-0002-8615-5331
Türkiye


Deniz YÜZBAŞIOĞLU>
Gazi University, Science Faculty, Department of Biology
0000-0003-2756-7712
Türkiye


Sabire Nur BÜLBÜL>
Gazi University, Science Faculty, Department of Biology
0000-0002-6897-793X
Türkiye


Fatma ÜNAL>
Gazi University, Science Faculty, Department of Biology
0000-0002-7468-6186
Türkiye

Supporting Institution This study was financially supported (except for comet assay) by Gazi University Scientific Research Projects Coordination Unit
Project Number 64/2020-01.
Publication Date October 1, 2022
Application Date June 7, 2022
Acceptance Date July 19, 2022
Published in Issue Year 2022, Volume 8, Issue 4

Cite

Bibtex @research article { jfhs1127611, journal = {Food and Health}, eissn = {2602-2834}, address = {Vidin Caddesi No:28 D:4 Kocamustafapaşa 34107 Fatih İstanbul}, publisher = {Özkan ÖZDEN}, year = {2022}, volume = {8}, number = {4}, pages = {273 - 283}, doi = {10.3153/FH22025}, title = {Investigation of cyto-genotoxic effects of a food sweetener Acesulfame potassium}, key = {cite}, author = {Mamur, Sevcan and Yüzbaşıoğlu, Deniz and Bülbül, Sabire Nur and Ünal, Fatma} }
APA Mamur, S. , Yüzbaşıoğlu, D. , Bülbül, S. N. & Ünal, F. (2022). Investigation of cyto-genotoxic effects of a food sweetener Acesulfame potassium . Food and Health , 8 (4) , 273-283 . DOI: 10.3153/FH22025
MLA Mamur, S. , Yüzbaşıoğlu, D. , Bülbül, S. N. , Ünal, F. "Investigation of cyto-genotoxic effects of a food sweetener Acesulfame potassium" . Food and Health 8 (2022 ): 273-283 <http://jfhs.scientificwebjournals.com/en/pub/issue/72186/1127611>
Chicago Mamur, S. , Yüzbaşıoğlu, D. , Bülbül, S. N. , Ünal, F. "Investigation of cyto-genotoxic effects of a food sweetener Acesulfame potassium". Food and Health 8 (2022 ): 273-283
RIS TY - JOUR T1 - Investigation of cyto-genotoxic effects of a food sweetener Acesulfame potassium AU - SevcanMamur, DenizYüzbaşıoğlu, Sabire NurBülbül, FatmaÜnal Y1 - 2022 PY - 2022 N1 - doi: 10.3153/FH22025 DO - 10.3153/FH22025 T2 - Food and Health JF - Journal JO - JOR SP - 273 EP - 283 VL - 8 IS - 4 SN - -2602-2834 M3 - doi: 10.3153/FH22025 UR - https://doi.org/10.3153/FH22025 Y2 - 2022 ER -
EndNote %0 Food and Health Investigation of cyto-genotoxic effects of a food sweetener Acesulfame potassium %A Sevcan Mamur , Deniz Yüzbaşıoğlu , Sabire Nur Bülbül , Fatma Ünal %T Investigation of cyto-genotoxic effects of a food sweetener Acesulfame potassium %D 2022 %J Food and Health %P -2602-2834 %V 8 %N 4 %R doi: 10.3153/FH22025 %U 10.3153/FH22025
ISNAD Mamur, Sevcan , Yüzbaşıoğlu, Deniz , Bülbül, Sabire Nur , Ünal, Fatma . "Investigation of cyto-genotoxic effects of a food sweetener Acesulfame potassium". Food and Health 8 / 4 (October 2022): 273-283 . https://doi.org/10.3153/FH22025
AMA Mamur S. , Yüzbaşıoğlu D. , Bülbül S. N. , Ünal F. Investigation of cyto-genotoxic effects of a food sweetener Acesulfame potassium. Food Health. 2022; 8(4): 273-283.
Vancouver Mamur S. , Yüzbaşıoğlu D. , Bülbül S. N. , Ünal F. Investigation of cyto-genotoxic effects of a food sweetener Acesulfame potassium. Food and Health. 2022; 8(4): 273-283.
IEEE S. Mamur , D. Yüzbaşıoğlu , S. N. Bülbül and F. Ünal , "Investigation of cyto-genotoxic effects of a food sweetener Acesulfame potassium", Food and Health, vol. 8, no. 4, pp. 273-283, Oct. 2022, doi:10.3153/FH22025

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