Investigation of cyto-genotoxic effects of a food sweetener Acesulfame potassium
Year 2022,
Volume: 8 Issue: 4, 273 - 283, 01.10.2022
Sevcan Mamur
,
Deniz Yüzbaşıoğlu
,
Sabire Nur Bülbül
,
Fatma Ünal
Abstract
Acesulfame potassium (ACE-K) is an artificial sweetener widely used in many foods. This investigation assessed the cytotoxic effect of ACE-K using MTT assay in human hepatocellular carcinoma (HepG2) cell line and the genotoxic effect using chromosomal aberrations (CAs), micronucleus (MN), and comet assays in human lymphocytes. 7.5-240 μg/mL concentrations of ACE-K were applied to cells. ACE-K notably decreased the cell viability on HepG2 cells, especially at 120 and 240 μg/mL at 24 and 48 h. It also significantly reduced the mitotic index (MI) at 60, 120, and 240 μg/mL at both treatments (24 and 48 h) in human lymphocytes. The frequency of the CAs significantly increased at 60, 120, and 240 μg/mL for 48 h treatment compared to control. However, no difference was observed in the frequency of MN and nuclear division index (NDI) at all the treatments. ACE-K also induced comet tail length, tail intensity, and moment at 15 μg/mL in isolated human lymphocytes. Therefore, ACE-K showed a cytotoxic effect in HepG2 cells as well as human lymphocytes at higher concentrations. It also exhibits a mild genotoxic effect by increasing the frequency of CAs at long-term treatment and DNA damaging effect only at 15 μg/mL.
Supporting Institution
This study was financially supported (except for comet assay) by Gazi University Scientific Research Projects Coordination Unit
Project Number
64/2020-01.
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Year 2022,
Volume: 8 Issue: 4, 273 - 283, 01.10.2022
Sevcan Mamur
,
Deniz Yüzbaşıoğlu
,
Sabire Nur Bülbül
,
Fatma Ünal
Project Number
64/2020-01.
References
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- Fındıklı, Z., Türkoglu, S., (2014). Determination of the effects of some artificial sweeteners on human peripheral lymphocytes using the comet assay. Journal of Toxicology and Environmental Health Sciences, 6(8), 147-153. https://doi.org/10.5897/JTEHS2014.0313
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- Fowler, S.P.G., (2016). Low-calorie sweetener use and energy balance: Results from experimental studies in animals, and large-scale prospective studies in humans. Physiology Behavior, 164, 517-523. https://doi.org/10.1016/j.physbeh.2016.04.047
- Fung, T.T., Malik, V., Rexrode, K.M., Manson, J.E., Willett, W.C., Hu, F.B. (2009). Sweetened beverage consumption and risk of coronary heart disease in women. The American Journal of Clinical Nutrition, 89(4), 1037-1042. https://doi.org/10.3945/ajcn.2008.27140
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- Hernández-Pérez, A.F., Jofre, F.M., Queiroz, S., de Arruda, P.V., Chandel, A.K., Graç, M.D., Almeida, F. (2020). Chapter 9: Biotechnological production of sweeteners. Verma, M., Chandel, A., (Eds). Biotechnological Production of Bioactive Compounds, Publisher: Elsevier, 261-292. https://doi.org/10.1016/B978-0-444-64323-0.00009-6
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- Jeffrey, A.M., Williams, G.M. (2000). Lack of DNA-damaging activity of five non-nutritive sweeteners in the rat hepatocyte/DNA repair assay. Food and Chemical Toxicology, 38(4), 335-338. https://doi.org/10.1016/S0278-6915(99)00163-5
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