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Research Article
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Antibacterial and antioxidant activity of Black, Red, and Chinese cultivars of Raphanus sativus

Year 2025, Volume: 11 Issue: 4, 301 - 317

Abdulmajeed Khalaf , Alican Bahadır Semerci , Şule Baran

Abstract

Our study sought to ascertain the biological activities of three distinct radish cultivars: Black, Red, and Chinese. The materials were subjected to lyophilisation for 72 hours, after which ethanolic extracts were obtained via the Soxhlet method. The antioxidant activity of the ethanolic extracts was assessed using the DPPH scavenging assay and the ferric reducing assay. At the same time, the total phenolic content was quantified by the Folin-Ciocalteu method. The antibacterial efficacy of the extracts against the test bacteria was assessed via the disc diffusion method. The findings revealed that Chinese radish exhibited the highest phenolic concentration, quantified at 18.1 mg GAE/g. The DPPH scavenging assay results indicated that, concerning the total phenolic content, the maximum scavenging activity was seen in Chinese radish at 72%. Subsequently, black radish exhibited a DPPH scavenging rate of 59%, while red radish demonstrated a rate of 39%. The ferric reducing power results corroborated the DPPH scavenging assay, indicating the activity hierarchy as Chinese radish, black radish, and red radish, respectively. Antibacterial activity of the ethanolic extracts obtained was also examined; according to the results, only the black radish extract showed antibacterial action against Enterococcus faecalis (13.5 mm) and Staphylococcus aureus (11 mm). This study revealed the effects of different radish cultivars on antioxidant and antibacterial activity.

Keywords

Antibacterial activity DPPH scavenging Food Radish

Ethical Statement

The authors declare that this study does not involve experiments with human or animal subjects, and therefore, ethics committee approval is not required.

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Details

Primary Language English
Subjects Food Biotechnology, Food Sciences (Other)
Journal Section Research Articles
Authors

Abdulmajeed Khalaf 0009-0007-1490-7278

Alican Bahadır Semerci 0000-0001-9502-9321

Şule Baran 0000-0003-2497-5876

Early Pub Date August 12, 2025
Publication Date August 30, 2025
Submission Date February 10, 2025
Acceptance Date May 7, 2025
Published in Issue Year 2025 Volume: 11 Issue: 4

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APA Khalaf, A., Semerci, A. B., & Baran, Ş. (2025). Antibacterial and antioxidant activity of Black, Red, and Chinese cultivars of Raphanus sativus. Food and Health, 11(4), 301-317.
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