Year 2019, Volume 5 , Issue 3, Pages 149 - 159 2019-07-01

IDENTIFICATION OF Staphylococcus aureus CHEESE ISOLATES WITH RESPECT TO VIRULENCE PROPERTIES, GENETIC RELATEDNESS AND ANTIBIOTIC RESISTANCE PROFILES

Pınar Kadiroğlu [1] , Figen Korel [2] , Çağatay Ceylan [3]


The problems on identification of Staphylococcus aureus isolates from cheese samples were investigated by phenotypic and genotypic tests in this study. Among 207 Staphylococcus spp. isolated from 31 cheese samples, 23 isolates that were Gram positive, catalase and slide coagulase positive, with 1 isolate that was latex agglutination test negative showed different phenotypic properties. Polymerase chain reaction (PCR) and quantitative PCR (qPCR) analyses showed that DNase test and target genes (nuc, coa) regarded as gold standard regions for S. aureus were not found to be unique for identification of S. aureus. The toxin genes (SEA-SEE) were not detected by PCR. Antibiotic resistance profiles of S. aureus isolates demonstrated that two isolates were resistant to penicillin G. This study showed that the unique phenotypic and genotypic test was not adequate for identification of S. aureus isolates. There was no correlation between the presence of the nuc gene and toxin genes. The presence of nuc gene which was used for detection of S. aureus was also found to be present in other Staphylococcus isolates. As a conclusion, the results revealed that biochemical tests could lead to false positive results for identification of S. aureus. The presence of nuc gene is not correlated with the presence of toxin genes.

Staphylococcus aureus, PCR, Identification, Antibiotic resistance
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Primary Language en
Subjects Food Science and Technology
Journal Section Research Articles
Authors

Orcid: 0000-0002-9730-8655
Author: Pınar Kadiroğlu (Primary Author)
Institution: Adana Science and Technology University, Food Engineering Department, Sarıçam, Adana
Country: Turkey


Orcid: 0000-0001-8202-6797
Author: Figen Korel (Primary Author)
Institution: İzmir Institute of Technology, Food Engineering Department, Urla, İzmir
Country: Turkey


Orcid: 0000-0001-5254-5983
Author: Çağatay Ceylan
Institution: İzmir Institute of Technology, Food Engineering Department, Urla, İzmir,
Country: Turkey


Dates

Publication Date : July 1, 2019

Bibtex @research article { jfhs469839, journal = {Food and Health}, issn = {}, eissn = {2602-2834}, address = {Vidin Caddesi No:28 D:4 Kocamustafapaşa 34107 Fatih İstanbul}, publisher = {ScientificWebJournals}, year = {2019}, volume = {5}, pages = {149 - 159}, doi = {10.3153/FH19016}, title = {IDENTIFICATION OF Staphylococcus aureus CHEESE ISOLATES WITH RESPECT TO VIRULENCE PROPERTIES, GENETIC RELATEDNESS AND ANTIBIOTIC RESISTANCE PROFILES}, key = {cite}, author = {Kadiroğlu, Pınar and Korel, Figen and Ceylan, Çağatay} }
APA Kadiroğlu, P , Korel, F , Ceylan, Ç . (2019). IDENTIFICATION OF Staphylococcus aureus CHEESE ISOLATES WITH RESPECT TO VIRULENCE PROPERTIES, GENETIC RELATEDNESS AND ANTIBIOTIC RESISTANCE PROFILES. Food and Health , 5 (3) , 149-159 . DOI: 10.3153/FH19016
MLA Kadiroğlu, P , Korel, F , Ceylan, Ç . "IDENTIFICATION OF Staphylococcus aureus CHEESE ISOLATES WITH RESPECT TO VIRULENCE PROPERTIES, GENETIC RELATEDNESS AND ANTIBIOTIC RESISTANCE PROFILES". Food and Health 5 (2019 ): 149-159 <http://jfhs.scientificwebjournals.com/en/issue/42227/469839>
Chicago Kadiroğlu, P , Korel, F , Ceylan, Ç . "IDENTIFICATION OF Staphylococcus aureus CHEESE ISOLATES WITH RESPECT TO VIRULENCE PROPERTIES, GENETIC RELATEDNESS AND ANTIBIOTIC RESISTANCE PROFILES". Food and Health 5 (2019 ): 149-159
RIS TY - JOUR T1 - IDENTIFICATION OF Staphylococcus aureus CHEESE ISOLATES WITH RESPECT TO VIRULENCE PROPERTIES, GENETIC RELATEDNESS AND ANTIBIOTIC RESISTANCE PROFILES AU - Pınar Kadiroğlu , Figen Korel , Çağatay Ceylan Y1 - 2019 PY - 2019 N1 - doi: 10.3153/FH19016 DO - 10.3153/FH19016 T2 - Food and Health JF - Journal JO - JOR SP - 149 EP - 159 VL - 5 IS - 3 SN - -2602-2834 M3 - doi: 10.3153/FH19016 UR - https://doi.org/10.3153/FH19016 Y2 - 2018 ER -
EndNote %0 Food and Health IDENTIFICATION OF Staphylococcus aureus CHEESE ISOLATES WITH RESPECT TO VIRULENCE PROPERTIES, GENETIC RELATEDNESS AND ANTIBIOTIC RESISTANCE PROFILES %A Pınar Kadiroğlu , Figen Korel , Çağatay Ceylan %T IDENTIFICATION OF Staphylococcus aureus CHEESE ISOLATES WITH RESPECT TO VIRULENCE PROPERTIES, GENETIC RELATEDNESS AND ANTIBIOTIC RESISTANCE PROFILES %D 2019 %J Food and Health %P -2602-2834 %V 5 %N 3 %R doi: 10.3153/FH19016 %U 10.3153/FH19016
ISNAD Kadiroğlu, Pınar , Korel, Figen , Ceylan, Çağatay . "IDENTIFICATION OF Staphylococcus aureus CHEESE ISOLATES WITH RESPECT TO VIRULENCE PROPERTIES, GENETIC RELATEDNESS AND ANTIBIOTIC RESISTANCE PROFILES". Food and Health 5 / 3 (July 2019): 149-159 . https://doi.org/10.3153/FH19016
AMA Kadiroğlu P , Korel F , Ceylan Ç . IDENTIFICATION OF Staphylococcus aureus CHEESE ISOLATES WITH RESPECT TO VIRULENCE PROPERTIES, GENETIC RELATEDNESS AND ANTIBIOTIC RESISTANCE PROFILES. Food Health. 2019; 5(3): 149-159.
Vancouver Kadiroğlu P , Korel F , Ceylan Ç . IDENTIFICATION OF Staphylococcus aureus CHEESE ISOLATES WITH RESPECT TO VIRULENCE PROPERTIES, GENETIC RELATEDNESS AND ANTIBIOTIC RESISTANCE PROFILES. Food and Health. 2019; 5(3): 159-149.