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Research Article
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PRELIMINARY STUDY OF DNA ЕXTRACTION FROM BULGARIAN HONEYS AND ITS AMPLIFICATION BY PCR FOR BOTANICAL IDENTIFICATION

Year 2018, Volume: 4 Issue: 3, 194 - 201, 01.07.2018

Ralitsa Balkanska , Katerina Stefanova Radostina Stoikova – Grigorova Vanya Manolova

https://doi.org/10.3153/FH18020

Abstract

Some current commonly used methods for identification of botanical
origin of honey involve melissopalynological analysis or other analytical
methods. However, these methods can be less sensitive and time-consuming.
DNA-based methods have become popular due to their quickness and reliability.
In this respect, the purpose on the present study is to compare two DNA
extraction kits – DNeasy Plant Mini Kit (Qiagen) and GeneJET Genomic DNA
Purification Mini Kit (Thermo Scientific) and CTAB method combined with
different pre-treatment procedures and applied to 3 multifloral and 2
monofloral honey samples. The DNA extraction and amplification was carried out
for all honey samples with similar results in each replication. The extracted
DNA was amplified by PCR using plant specific primers rbcL gene and trnH-psbA
spacer. The result obtained confirming that the DNA extracted has good quality
for analysis of PCR products and that it can be used for botanical
identification of honey. 

Keywords

rbcL trnH-psbA spacer DNA еxtraction Honey Botanical identification

References

  • Atanassova, J., Bozilova, E., Todorova, S. (2004). Pollen analysis of honey from the region of three villages in West Bulgaria. Phytologia Balcanica, 10(2-3), 247-252.
  • Atanassova, J., Kondova, V. (2004). Pollen and chemical-physical analysis of unifloral honey from different regions of Bulgaria. Phytologia Balcanica, 10(1), 45-50. Atanassova, J., Yurukova, L., & Lazarova, M. (2009). Palynological, physical, and chemical data on honey from the Kazanlak region (Central Bulgaria). Phytologia Balcanica, 15(1), 107-114. Atanassova, J., Yurukova, L., Lazarova, M. (2012). Pollen and inorganic characteristics of Bulgarian unifloral honeys. Czech Journal of Food Sciences, 30(6), 520-526. Bruni, I., De Mattia, F., Martellos, S., Galimberti, A., Savadori, P. Casiraghi, M., Nimis, P.L., Labra, M. (2012). DNA Barcoding as an effective tool in improving a digital plant identification system: A case study for the area of Mt. Valerio, Trieste (NE Italy). PLoS ONE 7(9): e43256.
  • Bruni, I., Galimberti, A., Caridi, L., Scaccabarozzi, D., De Mattia, F. (2015). A DNA barcoding approach to identify plant species in multiflower honey. Food Chemistry, 170(1), 308-315.
  • Bulgarian State Standard (1980). Rules for sampling and tasting methods. Qualitative indicators on honey 3050-80. http://www.bds-bg.org/bg/standard/?natstandard_document_id=61915 (accessed 18.10.2017)
  • Bulgarian State Standard (1980). Bee Honey 2673-80. De Mattia, F., Gentili, R., Bruni, I., Galimberti, A., Sgorbati S. (2012). A multi-marker DNA barcoding approach to save time and resources in vegetation surveys. Botanical Journal of the Linnean Society, 169, 518–529. Fernandez-Torres, R., Pérez-Bernal, J., Bello-Lopez, M., Callejón-Mochón, M., Jimenez-Sanchez, J., Guiraum-Perez, A. (2005). Mineral content and botanical origin of Spanish honeys. Talanta, 65, 686-691.
  • Galimberti, A., De Mattia, F., Bruni, I., Scaccabarozzi, D., Sandionigi, A., Barbuto, M., Casiraghi, M., Labra, M. (2014). A DNA barcoding approach to characterize pollen collected by honeybees. PLoS ONE 9(10): e109363. Guyot, C., Scheirman, V., Collin, S. (1999). Floral origin markers of heather honeys: Calluna vulgaris and Erica arborea. Food Chemistry, 64, 3-11.
  • Hernandez, O.М., Fraga, J.M.G., Jiménez, A.I., Arias, J.J. (2005). Characterization of honey from the Canary Islands: Determination of the mineral content by atomic absorption spectrophotometry. Food Chemistry, 93, 449-458. Lalhmangaihi, R., Ghatak, S., Laha, R., Gurusubramanian, G., Kumar, N. (2014). Protocol for optimal Quality and Quantity Pollen DNA isolation from Honey samples. Journal of Biomolecular Thechniques, 25, 92-95.
  • Latorre, M.J., Pena, R., Pita, C., Botana, A., Garcia, S., Herrero, C. (1999). Chemometric classification of honeys according to their type. II. Metal content data. Food Chemistry, 66, 263-268. Liberato, M., Morais, S., Magalhaes, C., Magalhaes, I., Cavalcanti, D., Silva, M. (2013). Physicochemical properties and mineral and protein content of honey samples from Ceará State, Northeastern Brazil. Food Science and Technology (Campinas), 33(1), 38-46.
  • Longhi, S., Cristofori, A., Gatto, P., Cristofolini, F., Grando, M.S., Gottardini, Е. (2009). Biomolecular identification of allergenic pollen: A new perspective for aerobiological monitoring. Annals of Allergy, Asthma and Immunology, 103, 508-514.
  • Matsuki, Y.R., Tateno, R., Shibata, M., Isagi, Y. (2008). Pollination efficiencies of flowervisiting insects as determined by direct genetic analysis of pollen origin. American Journal of Botany, 95, 925-930.
  • Murray, H.G., Tompson, W.F. (1980). Rapid isolation of high molecular weight plant DNA. Nucleic acids Research, 8(19), 4321-4325. Parducci, L., Matetovici, I., Fontana, S.L., Bennet, K.D.Y., Suyama, Y., Haile, J., Kurt, H., Kjaer, K.H., Larsen, N., Drouzas, A., Willerslev, E. (2012). Molecular and pollen-based vegetation analysis in lake sediments from Central Scandinavia. Molecular Ecology, 22, 3511-3524.
  • Radovic, B.S., Careri, M., Mangia, A., Musci, M., Gerboles, M., Anklam, E. 2001. Contribution of dynamic head space GC–MS analysis of aroma compounds to authenticity testing of honey. Food Chemistry, 72, 511-520.
  • Soares, S., Amaral, J.S., Oliveira, M.B.P.P., Mafra, I. (2015). Improving DNA isolation from honey for the botanical origin identification. Food Control, 48, 130-136.
  • Soria, A.C., Gonzalez, M., De Lorenzo, C., Martínez-Castro, I., Sanz, J. (2004). Characterization of artisanal honeys from Madrid (Central Spain) on the basis of their melissopaynological, physicochemical and volatile data. Food Chemistry, 85, 121-130. Wang, J., Li, Q.X. (2011). Chemical composition, characterization, and differentiation of honey botanical and geographical origins. Advances in Food and Nutrition Research, 62, 89-137. Wilson, E.E., Sidhu, C.S., Le Van, K.E., Holway, D.A. (2010). Pollen foraging behavior of solitary Hawaiian bees revealed through molecular pollen analysis. Molecular Ecology, 19, 4823-4829.

Year 2018, Volume: 4 Issue: 3, 194 - 201, 01.07.2018

Ralitsa Balkanska , Katerina Stefanova Radostina Stoikova – Grigorova Vanya Manolova

https://doi.org/10.3153/FH18020

Abstract

References

  • Atanassova, J., Bozilova, E., Todorova, S. (2004). Pollen analysis of honey from the region of three villages in West Bulgaria. Phytologia Balcanica, 10(2-3), 247-252.
  • Atanassova, J., Kondova, V. (2004). Pollen and chemical-physical analysis of unifloral honey from different regions of Bulgaria. Phytologia Balcanica, 10(1), 45-50. Atanassova, J., Yurukova, L., & Lazarova, M. (2009). Palynological, physical, and chemical data on honey from the Kazanlak region (Central Bulgaria). Phytologia Balcanica, 15(1), 107-114. Atanassova, J., Yurukova, L., Lazarova, M. (2012). Pollen and inorganic characteristics of Bulgarian unifloral honeys. Czech Journal of Food Sciences, 30(6), 520-526. Bruni, I., De Mattia, F., Martellos, S., Galimberti, A., Savadori, P. Casiraghi, M., Nimis, P.L., Labra, M. (2012). DNA Barcoding as an effective tool in improving a digital plant identification system: A case study for the area of Mt. Valerio, Trieste (NE Italy). PLoS ONE 7(9): e43256.
  • Bruni, I., Galimberti, A., Caridi, L., Scaccabarozzi, D., De Mattia, F. (2015). A DNA barcoding approach to identify plant species in multiflower honey. Food Chemistry, 170(1), 308-315.
  • Bulgarian State Standard (1980). Rules for sampling and tasting methods. Qualitative indicators on honey 3050-80. http://www.bds-bg.org/bg/standard/?natstandard_document_id=61915 (accessed 18.10.2017)
  • Bulgarian State Standard (1980). Bee Honey 2673-80. De Mattia, F., Gentili, R., Bruni, I., Galimberti, A., Sgorbati S. (2012). A multi-marker DNA barcoding approach to save time and resources in vegetation surveys. Botanical Journal of the Linnean Society, 169, 518–529. Fernandez-Torres, R., Pérez-Bernal, J., Bello-Lopez, M., Callejón-Mochón, M., Jimenez-Sanchez, J., Guiraum-Perez, A. (2005). Mineral content and botanical origin of Spanish honeys. Talanta, 65, 686-691.
  • Galimberti, A., De Mattia, F., Bruni, I., Scaccabarozzi, D., Sandionigi, A., Barbuto, M., Casiraghi, M., Labra, M. (2014). A DNA barcoding approach to characterize pollen collected by honeybees. PLoS ONE 9(10): e109363. Guyot, C., Scheirman, V., Collin, S. (1999). Floral origin markers of heather honeys: Calluna vulgaris and Erica arborea. Food Chemistry, 64, 3-11.
  • Hernandez, O.М., Fraga, J.M.G., Jiménez, A.I., Arias, J.J. (2005). Characterization of honey from the Canary Islands: Determination of the mineral content by atomic absorption spectrophotometry. Food Chemistry, 93, 449-458. Lalhmangaihi, R., Ghatak, S., Laha, R., Gurusubramanian, G., Kumar, N. (2014). Protocol for optimal Quality and Quantity Pollen DNA isolation from Honey samples. Journal of Biomolecular Thechniques, 25, 92-95.
  • Latorre, M.J., Pena, R., Pita, C., Botana, A., Garcia, S., Herrero, C. (1999). Chemometric classification of honeys according to their type. II. Metal content data. Food Chemistry, 66, 263-268. Liberato, M., Morais, S., Magalhaes, C., Magalhaes, I., Cavalcanti, D., Silva, M. (2013). Physicochemical properties and mineral and protein content of honey samples from Ceará State, Northeastern Brazil. Food Science and Technology (Campinas), 33(1), 38-46.
  • Longhi, S., Cristofori, A., Gatto, P., Cristofolini, F., Grando, M.S., Gottardini, Е. (2009). Biomolecular identification of allergenic pollen: A new perspective for aerobiological monitoring. Annals of Allergy, Asthma and Immunology, 103, 508-514.
  • Matsuki, Y.R., Tateno, R., Shibata, M., Isagi, Y. (2008). Pollination efficiencies of flowervisiting insects as determined by direct genetic analysis of pollen origin. American Journal of Botany, 95, 925-930.
  • Murray, H.G., Tompson, W.F. (1980). Rapid isolation of high molecular weight plant DNA. Nucleic acids Research, 8(19), 4321-4325. Parducci, L., Matetovici, I., Fontana, S.L., Bennet, K.D.Y., Suyama, Y., Haile, J., Kurt, H., Kjaer, K.H., Larsen, N., Drouzas, A., Willerslev, E. (2012). Molecular and pollen-based vegetation analysis in lake sediments from Central Scandinavia. Molecular Ecology, 22, 3511-3524.
  • Radovic, B.S., Careri, M., Mangia, A., Musci, M., Gerboles, M., Anklam, E. 2001. Contribution of dynamic head space GC–MS analysis of aroma compounds to authenticity testing of honey. Food Chemistry, 72, 511-520.
  • Soares, S., Amaral, J.S., Oliveira, M.B.P.P., Mafra, I. (2015). Improving DNA isolation from honey for the botanical origin identification. Food Control, 48, 130-136.
  • Soria, A.C., Gonzalez, M., De Lorenzo, C., Martínez-Castro, I., Sanz, J. (2004). Characterization of artisanal honeys from Madrid (Central Spain) on the basis of their melissopaynological, physicochemical and volatile data. Food Chemistry, 85, 121-130. Wang, J., Li, Q.X. (2011). Chemical composition, characterization, and differentiation of honey botanical and geographical origins. Advances in Food and Nutrition Research, 62, 89-137. Wilson, E.E., Sidhu, C.S., Le Van, K.E., Holway, D.A. (2010). Pollen foraging behavior of solitary Hawaiian bees revealed through molecular pollen analysis. Molecular Ecology, 19, 4823-4829.
There are 14 citations in total.

Details

Primary Language English
Subjects Food Engineering
Journal Section Articles
Authors

Ralitsa Balkanska 0000-0003-3486-1514

Katerina Stefanova 0000-0002-0327-4408

Radostina Stoikova – Grigorova 0000-0003-0881-7827

Vanya Manolova 0000-0001-6962-3562

Publication Date July 1, 2018
Submission Date October 29, 2017
Published in Issue Year 2018 Volume: 4 Issue: 3

Cite

APA Balkanska, R., Stefanova, K., Stoikova – Grigorova, R., Manolova, V. (2018). PRELIMINARY STUDY OF DNA ЕXTRACTION FROM BULGARIAN HONEYS AND ITS AMPLIFICATION BY PCR FOR BOTANICAL IDENTIFICATION. Food and Health, 4(3), 194-201. https://doi.org/10.3153/FH18020
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