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Research Article
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Nigella sativa OIL COULD INDUCE OSTEOGENIC DIFFERENTIATION OF DENTAL PULP MESENCHYMAL STEM CELLS: CLINICAL NUTRITION FOR DENTISTRY

Year 2018, Volume: 4 Issue: 1, 19 - 24, 01.01.2018

Ayşegül Mendi

https://doi.org/10.3153/JFHS18003

Abstract

A
natural agent that, promotes osteogenic differentiation of dental pulp
mesenchymal stem cells (DP-MSCs) could achieve success in regeneration during
healing and may also prevent bone resorption and improve regeneration. We aimed
to demonstrate that a Nigella sativa
oil could induce differentiation of DP-MSCs. DP-MSCs were isolated from 3.
molars and identified with flow cytometer. Osteogenic differentiation was
conducted, and calcium granules were showed by typical Alizarin Red dying and
calcium concentration were determined by DICA 500. Identification results
showed that the cells are mesenchymal stem cells. Alizarin Red dying signed the
calcium granules and DICA 500 showed that calcium concentrations were higher
than the Control Group. This preliminary study shows for the first time the
inductive potential of N. sativa oil
for osteogenic differentiation in DP-MSCs. The mechanism should be investigated
in further studies. 

Keywords

Dental pulp mesenchymal stem cells Nigella sativa Osteogenic differentiation Dexamethosone

References

  • Abd-Awn, B., Al-Dhaher, Z. & Al-Dafaai, R. (2012). The effect of black seed oil extracts on mutans streptococci in comparison to chlorhexidine gluconate (in vitro). Journal of Baghdad College of Dentistry, 24, 126-131.
  • Ali, B.H. & Blunden, G. (2003). Pharmacological and toxicological properties of Nigella sativa. Phytother. Res. 17, 299-305.
  • Kirui, P.K., Cameron, J., Benghuzzi, H.A., Tucci, M., Patel, R., Adah, F. & Russell, G. (2004). Effects of sustained delivery of thymoqiunone on bone healing of male rats. Biomedical Science Instrumentation, 40, 111-116.
  • D'aquino, R., Grazıano, A., Sampaolesi, M., Laino, G., Pırozzı, G., De Rosa, A. & Papaccio, G. (2007). Human postnatal dental pulp cells co-differentiate into osteoblasts and endotheliocytes: a pivotal synergy leading to adult bone tissue formation. Cell Death & Differentiation. 14, 1162–1171.
  • Egermann, M., Goldhahn, J. & Schneider, E. (2005). Animal models for fracture treatment in osteoporosis. Osteoporos International, 16(suppl2), 129-138.
  • Gali-Muhtasib, H., Roessner, A. & Schneider-Stock, R. (2005). Thymoquinone: a promising anti-cancer drug from natural sources. The International Journal of Biochemistry & Cell Biology, 38, 1249-1253.
  • Gronthos, S., Mankani, M., Brahim, J., Robey, P.G. & Shi, S. (2000). Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proceedings of the Natiıonal Academy of Science, 97, 13625-13630.
  • Harzallah, H., Kouidhi, B., Flamini, G., Bakhrouf, A. & Mahjoub, T. (2011). Chemical composition, antimicrobial potential against cariogenic bacteria and cytotoxic activity of Tunisian Nigella sativa essential oil and thymoquinone. Food Chemistry, 129,1469-1474.
  • Hoiruchi, K., Amizuka, N., Takeshita, S., Takamatsu, H., Katsuura, M., Ozawa, H., Toyama, Y., Bonewald, L.F. & Kudo, A. (1999). Identification and chracterization of a novel protein, perostin, with restricted expression to periosteum and periodontal ligament and increased expression by transforming growth factor beta. Journal of Bone & Mineral Research, 14,1239-1249.
  • Kaleem, M., Kirmani, D., Asif, M., Ahmed, Q., Bano, B. (2006). Biochemical effects of Nigella sativa L. seeds in diabetic rats. Indian Journal of Experimental Biology, 44, 745-748.
  • Kanter, M., Coşkun, O. & Uysal, H. (2006). The antioxidative and antihistaminic effect of Nigella sativa and its major constituent, thymoquinone on ethanol-induced gastric mucosal damage. Archves of Toxicology, 80, 217-224.
  • Mabrouk, G.M., Moselhy, S.S., Zohny, S.F., Ali, E.M., Helal, T.E., Amin, A.A. & Khalifa, A.A. (2002). Inhibition of methylnitrosourea (MNU) induced oxidative stress and carcinogenesis by orally administered bee honey and Nigella grains in Sprague Dawley rats. Journal of Experimental and Clinical Cancer Research, 21, 341-346.
  • Pisciota, A., Carnevale G., Meloni, S., Ricio, M., Biasi, S.D., Gibellini, L., Ferrari, A., Bruzzesi, G. & de Pol, A. (2015). Human dental pulp stem cells (hDPSCs): isolation, enrichment and comparative differentiation of two sub-populations. BMC Developmental Biology, 15, 14-16
  • Wirries, A., Schubert, A.K., Zimmermann, R., Jabari, S., Ruchholtz, S. & El-Najjar, N. (2013). Thymoquinone accelerates osteoblast differentiation and activates bone morphogenetic protein-2 and ERK pathway. International Immunopharmacology, 15(2), 381-386.
  • Ozdemir, H., Kara, M.I., Erciyas, K., Ozer, H. & Ay, S. (2012). Preventive effects of thymoquinone in a rat periodontitis model: a morphometric and histopathological study. Journal of Periodontal Research, 47, 74-80.
  • Pittenger, M.F., Mackay, A.M., Beck, S. (1999). Multilineage potential of adult human mesenchymal stem cells. Science, 284, 1168-1170.
  • Shaker, A. & Al-Wafi, H. (2014). Benefits of thymoquinone, a Nigella sativa extract in preventing dental caries initiation and improving gingival health. ProQuest LLC, 72.

Year 2018, Volume: 4 Issue: 1, 19 - 24, 01.01.2018

Ayşegül Mendi

https://doi.org/10.3153/JFHS18003

Abstract

References

  • Abd-Awn, B., Al-Dhaher, Z. & Al-Dafaai, R. (2012). The effect of black seed oil extracts on mutans streptococci in comparison to chlorhexidine gluconate (in vitro). Journal of Baghdad College of Dentistry, 24, 126-131.
  • Ali, B.H. & Blunden, G. (2003). Pharmacological and toxicological properties of Nigella sativa. Phytother. Res. 17, 299-305.
  • Kirui, P.K., Cameron, J., Benghuzzi, H.A., Tucci, M., Patel, R., Adah, F. & Russell, G. (2004). Effects of sustained delivery of thymoqiunone on bone healing of male rats. Biomedical Science Instrumentation, 40, 111-116.
  • D'aquino, R., Grazıano, A., Sampaolesi, M., Laino, G., Pırozzı, G., De Rosa, A. & Papaccio, G. (2007). Human postnatal dental pulp cells co-differentiate into osteoblasts and endotheliocytes: a pivotal synergy leading to adult bone tissue formation. Cell Death & Differentiation. 14, 1162–1171.
  • Egermann, M., Goldhahn, J. & Schneider, E. (2005). Animal models for fracture treatment in osteoporosis. Osteoporos International, 16(suppl2), 129-138.
  • Gali-Muhtasib, H., Roessner, A. & Schneider-Stock, R. (2005). Thymoquinone: a promising anti-cancer drug from natural sources. The International Journal of Biochemistry & Cell Biology, 38, 1249-1253.
  • Gronthos, S., Mankani, M., Brahim, J., Robey, P.G. & Shi, S. (2000). Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proceedings of the Natiıonal Academy of Science, 97, 13625-13630.
  • Harzallah, H., Kouidhi, B., Flamini, G., Bakhrouf, A. & Mahjoub, T. (2011). Chemical composition, antimicrobial potential against cariogenic bacteria and cytotoxic activity of Tunisian Nigella sativa essential oil and thymoquinone. Food Chemistry, 129,1469-1474.
  • Hoiruchi, K., Amizuka, N., Takeshita, S., Takamatsu, H., Katsuura, M., Ozawa, H., Toyama, Y., Bonewald, L.F. & Kudo, A. (1999). Identification and chracterization of a novel protein, perostin, with restricted expression to periosteum and periodontal ligament and increased expression by transforming growth factor beta. Journal of Bone & Mineral Research, 14,1239-1249.
  • Kaleem, M., Kirmani, D., Asif, M., Ahmed, Q., Bano, B. (2006). Biochemical effects of Nigella sativa L. seeds in diabetic rats. Indian Journal of Experimental Biology, 44, 745-748.
  • Kanter, M., Coşkun, O. & Uysal, H. (2006). The antioxidative and antihistaminic effect of Nigella sativa and its major constituent, thymoquinone on ethanol-induced gastric mucosal damage. Archves of Toxicology, 80, 217-224.
  • Mabrouk, G.M., Moselhy, S.S., Zohny, S.F., Ali, E.M., Helal, T.E., Amin, A.A. & Khalifa, A.A. (2002). Inhibition of methylnitrosourea (MNU) induced oxidative stress and carcinogenesis by orally administered bee honey and Nigella grains in Sprague Dawley rats. Journal of Experimental and Clinical Cancer Research, 21, 341-346.
  • Pisciota, A., Carnevale G., Meloni, S., Ricio, M., Biasi, S.D., Gibellini, L., Ferrari, A., Bruzzesi, G. & de Pol, A. (2015). Human dental pulp stem cells (hDPSCs): isolation, enrichment and comparative differentiation of two sub-populations. BMC Developmental Biology, 15, 14-16
  • Wirries, A., Schubert, A.K., Zimmermann, R., Jabari, S., Ruchholtz, S. & El-Najjar, N. (2013). Thymoquinone accelerates osteoblast differentiation and activates bone morphogenetic protein-2 and ERK pathway. International Immunopharmacology, 15(2), 381-386.
  • Ozdemir, H., Kara, M.I., Erciyas, K., Ozer, H. & Ay, S. (2012). Preventive effects of thymoquinone in a rat periodontitis model: a morphometric and histopathological study. Journal of Periodontal Research, 47, 74-80.
  • Pittenger, M.F., Mackay, A.M., Beck, S. (1999). Multilineage potential of adult human mesenchymal stem cells. Science, 284, 1168-1170.
  • Shaker, A. & Al-Wafi, H. (2014). Benefits of thymoquinone, a Nigella sativa extract in preventing dental caries initiation and improving gingival health. ProQuest LLC, 72.
There are 17 citations in total.

Details

Subjects Clinical Sciences
Journal Section Articles
Authors

Ayşegül Mendi 0000-0001-6705-3770

Publication Date January 1, 2018
Submission Date March 20, 2017
Published in Issue Year 2018 Volume: 4 Issue: 1

Cite

APA Mendi, A. (2018). Nigella sativa OIL COULD INDUCE OSTEOGENIC DIFFERENTIATION OF DENTAL PULP MESENCHYMAL STEM CELLS: CLINICAL NUTRITION FOR DENTISTRY. Food and Health, 4(1), 19-24. https://doi.org/10.3153/JFHS18003
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