LACTARIUS DELICIUS VE LACTARIUS SALMONICOLOR MANTARLARININ FENOLİK BİLEŞİKLERİ VE ANTİOKSİDAN ETKİLERİNİN DEĞERLENDİRİLMESİ
Öz
Amaç: Bu çalışmada, L. delicius ve L. salmonicolor mantarlarından elde edilen etanollü özütlerin fenolik içerikleri ve antioksidan enzim aktivitelerine olan etkilerinin incelenmesi amaçlanmıştır.
Gereç ve Yöntem: Mantar özütlerinin fenolik bileşiklerinin miktarı Folin-Ciocalteu, Alüminyum klorür kolorimetrik ve Yüksek Performanslı Sıvı Kromatografi (YPSK) yöntemleri ile analiz edilmiştir. Ayrıca, mantar özütlerinin antioksidan aktivite tayini DPPH yöntemi ile gerçekleştirilmiştir. Bununla birlikte mantarların etanollü özütlerinin glutatyon-S-transferaz (GST), glutatyon peroksidaz (GPx) ve katalaz (CAT) enzimleri üzerine olan etkileri araştırılmıştır.
Sonuç ve Tartışma: Elde edilen sonuçlar, L. delicius mantarından elde edilen etanollü özütün L. salmonicolor’a göre daha yüksek oranda fenolik bileşik içerdiğini ve antioksidan kapasitesinin de daha yüksek olduğunu göstermiştir.
Anahtar Kelimeler
Antioksidan fenolik bileşikler Lactarius delicius Lactarius salmonicolor
Destekleyen Kurum
TÜBİTAK
Proje Numarası
Proje No: 116Z125
Teşekkür
Bu çalışma, 3001 proje kapsamında (Proje No: 116Z125) TÜBİTAK tarafından desteklenmiştir.
Kaynakça
- 1. Eberhardt, U., Verbeken, A. (2004). Sequestrate Lactarius species from tropical Africa: L. angiocarpus sp. Nov. and L. dolichocaulis Comb. Nov. Mycological Research, 108(9), 1042-1052. [CrossRef]
- 2. Verbeken, A., Stubbe, D., Van de Putte, K., Eberhardt, U., Nuytinck, J. (2014). Tales of the unexpected: Angiocarpous representatives of the Russulaceae in tropical South East Asia. Persoonia: Molecular Phylogeny and Evolution of Fungi, 32, 13-24. [CrossRef]
- 3. Onbaşılı, D., Çelik, G., Katırcıoğlu, H., Narin, İ. (2015). Antimicrobial, antioxidant activities and chemical composition of Lactarius deliciosus (L.) collected from Kastamonu province of Turkey. Kastamonu Üniversitesi Orman Fakültesi Dergisi, 15(1), 98-103.
- 4. Ünal, S., Karadeniz, M. (2020). Kastamonu yöresinde tespit edilen Lactarius türleri. Ağaç ve Orman, 1(2), 50-58.
- 5. Shomali Moghaddam, N., Isgor, S.B., Isgor, Y.G., Geven, F., Yildirim, O. (2015). Evaluation of selected plants for their detoxifying effect via antioxidant defense system enzymes. Fresenius Environmental Bulletin, 24(1), 63-70.
- 6. Slinkard, K., Singleton, V.L. (1977). Total phenol analyses: Automation and comparison with manual methods. American Journal of Enology and Viticulture, 28, 49-55. [CrossRef]
- 7. Woisky, R., Salatino, A. (1998). Analysis of propolis: Some parameters and procedures for chemical quality control. Journal of Apicultural Research, 37, 99-105. [CrossRef]
- 8. Koç, S., İşgör, S.B., İşgör,Y.G., Shomali moghaddam, N., Altuner, E.M., Yıldırım, O. (2015). The potential medicinal value of plants from asteraceae family with antioxidant defense enzymes as biological targets. Pharmaceutical Biology, 53(5), 746-751. [CrossRef]
- 9. Habig, W.H., Pabst, M.J., Jakoby, W.B. (1974). Glutathione-S-transferases the first enzymatic step in mercapturic acid formation. The Journal of Biological Chemistry, 249(22-25), 7130-7139. [CrossRef]
- 10. Paglia, D.E. and Valentine, W.N. (1967). Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. Journal of Laboratory and Clinical Medicine, 70, 158-169.
- 11. Aebi, H. (1984). Catalase in vitro. Methods Enzymology, 105, 121-126. [CrossRef]
- 12. Kidd, P.M. (2000). The use of mushroom glucans and proteoglycans in cancer treatment. Alternative Medicine Review, 5, 4-27.
- 13. Sarikurkcu, C., Tepe, B., Semiz, D.K., Solak, M.H. (2010). Evaluation of metal concentration and antioxidant activity of three edible mushrooms from Mugla, Turkey. Food and Chemical Toxicology, 48, 1230-1233. [CrossRef]
- 14. Wright, J.S., Johnson, E.R., DiLabio, G.A. (2001). Predicting the activity of phenolic antioxidants: theoretical method, analysis of substituent effects, and application to major families of antioxidants. Journal of the American Chemical Society, 123, 1173-1183. [CrossRef]
- 15. Semwal, D.K., Semwal, R.B., Combrinck, S., Viljoen, A. (2016). Myricetin: a dietary molecule with diverse biological activities. Nutrients, 8, 90. [CrossRef]
- 16. Townsend, D.M., Tew, K.D. (2003). The role of glutathione-S-transferase in anti-cancer drug resistance. Oncogene, 22, 7369-7375. [CrossRef]
- 17. Cuia,Q., Wang, J.Q., Assarafc, Y., Rena, L., Gupta, P., Weid, L., Ashby,C.R., Yang, D.H., Chen, Z.S. (2018). Modulating ROS to overcome multidrug resistance in cancer. Drug Resistance Updates, 41, 1-25. [CrossRef]
- 18. Barros, L., Ferreira, M., Queiros, B., Ferreira, I.C.F.R., Baptista, P. (2007). Total phenols, ascorbic acid, β-carotene and lycopene in Portuguese wild edible mushrooms and their antioxidant activities. Food Chemistry, 103(2), 413-419. [CrossRef]
- 19. Kosanić, M., Ranković, B., Stanojković, T., Stošić, I., Grujičić, D., Milošević-Djordjević, O. (2016). Lasallia pustulata lichen as possible natural antigenotoxic, antioxidant, antimicrobial and anticancer agent. Cytotechnology, 68(4),999-1008. [CrossRef]
- 20. Ozen,T., Kizil, D., Yenigun, S., Cesur, H., Turkekul, I. (2019). Evaluation of bioactivities, phenolic and metal content of ten wild edible mushrooms from western black sea region of Turkey. International Journal of Medicinal Mushrooms, 21(10), 979-994. [CrossRef]
- 21. Kalogeropoulos, N., Yanni, A.E., Koutrotsios, G., Aloupi, M. (2013). Bioactive microconstituents and antioxidant properties of wild edible mushrooms from the island of Lesvos, Greece. Food and Chemical Toxicology, 55, 378-385. [CrossRef]
- 22. Athanasakis, G., Aligiannis, N., Gonou-Zagou, Z., Skaltsounis, A.L., Fokialakis, N. (2013). Antioxidant Properties of the Wild Edible Mushroom Lactarius salmonicolor. Journal of Medicinal Food, 16(8), 760-764. [CrossRef]
EVALUATION OF PHENOLIC COMPOUNDS AND ANTIOXIDANT EFFECTS OF LACTARIUS DELICIUS AND LACTARIUS SALMONICOLOR MUSHROOMS
Öz
Objective: In this study, it was aimed to investigate the phenolic content and antioxidant activities of ethanolic extracts obtained from L. delicius and L. salmonicolor mushrooms.
Material and Method: In this study, the amount of phenolic compounds of mushroom extracts were analyzed by Folin-Ciocalteu, Aluminum chloride colorimetric and High Performance Liquid Chromatography (HPLC) methods. In addition, the antioxidant activity of the mushroom extracts were determined by the DPPH method. The activity of ethanolic extracts of these mushrooms were also investigated on glutathione-S-transferase (GST), glutathione peroxidase (GPx) and catalase (CAT) enzymes.
Result and Discussion: The results showed that the ethanolic extract obtained from L. delicius mushroom contains higher phenolic compounds and higher antioxidant capacity than L. salmonicolor.
Anahtar Kelimeler
Antioxidant activity Lactarius delicius Lactarius salmonicolor phenolic compounds
Proje Numarası
Proje No: 116Z125
Kaynakça
- 1. Eberhardt, U., Verbeken, A. (2004). Sequestrate Lactarius species from tropical Africa: L. angiocarpus sp. Nov. and L. dolichocaulis Comb. Nov. Mycological Research, 108(9), 1042-1052. [CrossRef]
- 2. Verbeken, A., Stubbe, D., Van de Putte, K., Eberhardt, U., Nuytinck, J. (2014). Tales of the unexpected: Angiocarpous representatives of the Russulaceae in tropical South East Asia. Persoonia: Molecular Phylogeny and Evolution of Fungi, 32, 13-24. [CrossRef]
- 3. Onbaşılı, D., Çelik, G., Katırcıoğlu, H., Narin, İ. (2015). Antimicrobial, antioxidant activities and chemical composition of Lactarius deliciosus (L.) collected from Kastamonu province of Turkey. Kastamonu Üniversitesi Orman Fakültesi Dergisi, 15(1), 98-103.
- 4. Ünal, S., Karadeniz, M. (2020). Kastamonu yöresinde tespit edilen Lactarius türleri. Ağaç ve Orman, 1(2), 50-58.
- 5. Shomali Moghaddam, N., Isgor, S.B., Isgor, Y.G., Geven, F., Yildirim, O. (2015). Evaluation of selected plants for their detoxifying effect via antioxidant defense system enzymes. Fresenius Environmental Bulletin, 24(1), 63-70.
- 6. Slinkard, K., Singleton, V.L. (1977). Total phenol analyses: Automation and comparison with manual methods. American Journal of Enology and Viticulture, 28, 49-55. [CrossRef]
- 7. Woisky, R., Salatino, A. (1998). Analysis of propolis: Some parameters and procedures for chemical quality control. Journal of Apicultural Research, 37, 99-105. [CrossRef]
- 8. Koç, S., İşgör, S.B., İşgör,Y.G., Shomali moghaddam, N., Altuner, E.M., Yıldırım, O. (2015). The potential medicinal value of plants from asteraceae family with antioxidant defense enzymes as biological targets. Pharmaceutical Biology, 53(5), 746-751. [CrossRef]
- 9. Habig, W.H., Pabst, M.J., Jakoby, W.B. (1974). Glutathione-S-transferases the first enzymatic step in mercapturic acid formation. The Journal of Biological Chemistry, 249(22-25), 7130-7139. [CrossRef]
- 10. Paglia, D.E. and Valentine, W.N. (1967). Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. Journal of Laboratory and Clinical Medicine, 70, 158-169.
- 11. Aebi, H. (1984). Catalase in vitro. Methods Enzymology, 105, 121-126. [CrossRef]
- 12. Kidd, P.M. (2000). The use of mushroom glucans and proteoglycans in cancer treatment. Alternative Medicine Review, 5, 4-27.
- 13. Sarikurkcu, C., Tepe, B., Semiz, D.K., Solak, M.H. (2010). Evaluation of metal concentration and antioxidant activity of three edible mushrooms from Mugla, Turkey. Food and Chemical Toxicology, 48, 1230-1233. [CrossRef]
- 14. Wright, J.S., Johnson, E.R., DiLabio, G.A. (2001). Predicting the activity of phenolic antioxidants: theoretical method, analysis of substituent effects, and application to major families of antioxidants. Journal of the American Chemical Society, 123, 1173-1183. [CrossRef]
- 15. Semwal, D.K., Semwal, R.B., Combrinck, S., Viljoen, A. (2016). Myricetin: a dietary molecule with diverse biological activities. Nutrients, 8, 90. [CrossRef]
- 16. Townsend, D.M., Tew, K.D. (2003). The role of glutathione-S-transferase in anti-cancer drug resistance. Oncogene, 22, 7369-7375. [CrossRef]
- 17. Cuia,Q., Wang, J.Q., Assarafc, Y., Rena, L., Gupta, P., Weid, L., Ashby,C.R., Yang, D.H., Chen, Z.S. (2018). Modulating ROS to overcome multidrug resistance in cancer. Drug Resistance Updates, 41, 1-25. [CrossRef]
- 18. Barros, L., Ferreira, M., Queiros, B., Ferreira, I.C.F.R., Baptista, P. (2007). Total phenols, ascorbic acid, β-carotene and lycopene in Portuguese wild edible mushrooms and their antioxidant activities. Food Chemistry, 103(2), 413-419. [CrossRef]
- 19. Kosanić, M., Ranković, B., Stanojković, T., Stošić, I., Grujičić, D., Milošević-Djordjević, O. (2016). Lasallia pustulata lichen as possible natural antigenotoxic, antioxidant, antimicrobial and anticancer agent. Cytotechnology, 68(4),999-1008. [CrossRef]
- 20. Ozen,T., Kizil, D., Yenigun, S., Cesur, H., Turkekul, I. (2019). Evaluation of bioactivities, phenolic and metal content of ten wild edible mushrooms from western black sea region of Turkey. International Journal of Medicinal Mushrooms, 21(10), 979-994. [CrossRef]
- 21. Kalogeropoulos, N., Yanni, A.E., Koutrotsios, G., Aloupi, M. (2013). Bioactive microconstituents and antioxidant properties of wild edible mushrooms from the island of Lesvos, Greece. Food and Chemical Toxicology, 55, 378-385. [CrossRef]
- 22. Athanasakis, G., Aligiannis, N., Gonou-Zagou, Z., Skaltsounis, A.L., Fokialakis, N. (2013). Antioxidant Properties of the Wild Edible Mushroom Lactarius salmonicolor. Journal of Medicinal Food, 16(8), 760-764. [CrossRef]
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Konular | Eczacılık ve İlaç Bilimleri |
| Bölüm | Araştırma Makalesi |
| Yazarlar | |
| Proje Numarası | Proje No: 116Z125 |
| Erken Görünüm Tarihi | 17 Mayıs 2023 |
| Yayımlanma Tarihi | 20 Mayıs 2023 |
| Gönderilme Tarihi | 28 Aralık 2022 |
| Kabul Tarihi | 27 Mart 2023 |
| Yayımlandığı Sayı | Yıl 2023 Cilt: 47 Sayı: 2 |
Kaynak Göster
Kapsam ve Amaç
Ankara Üniversitesi Eczacılık Fakültesi Dergisi, açık erişim, hakemli bir dergi olup Türkçe veya İngilizce olarak farmasötik bilimler alanındaki önemli gelişmeleri içeren orijinal araştırmalar, derlemeler ve kısa bildiriler için uluslararası bir yayım ortamıdır. Bilimsel toplantılarda sunulan bildiriler supleman özel sayısı olarak dergide yayımlanabilir. Ayrıca, tüm farmasötik alandaki gelecek ve önceki ulusal ve uluslararası bilimsel toplantılar ile sosyal aktiviteleri içerir.