Araştırma Makalesi
PDF EndNote BibTex RIS Kaynak Göster

Cytotoxic effects of water and ethanolic extracts of Turkish propolis on human laryngeal epidermoid carcinoma cell lines

Yıl 2022, Cilt 8, Sayı 3, 218 - 228, 01.07.2022
https://doi.org/10.3153/FH22021

Öz

Propolis is a natural resinous substance collected by bees from various types of trees and plants and has antibacterial, antiviral and antitumoral features depending on its antioxidant properties. Major aim is to investigate cytotoxic effect of Turkish propolis on human laryngeal epidermoid carcinoma (HEp-2) cells. HEp-2 cells/well were loaded on RTCA(real time cell analysis) system and the cell index was followed up during 48 hours. Water extract of Turkish propolis (WEP) of 250-10.000 μg/mL concentrations and ethanolic extracts of Turkish propolis (EEP) of 10-2.400 μg/mL concentrations were treated with HEp-2 cells and followed by RTCA system. The cell indexes and IC50 values were determined. HEp-2 cells were incubated with WEP and EEP. Proliferation was followed by flow cytometric DNA cycle analysis. WEP and EEP were found to be cytotoxic to HEp-2 cells. When WEP and EEP were incubated with HEp-2 cells during 72 hours, the highest antiproliferative effect was seen by interfering DNA cycles. Turkish propolis extracts were found to be cytotoxic and antiproliferative to HEp- 2 cells in the present study, therefore, it was concluded that it may fall within chemotherapy or target therapies for larynx cancers.

Kaynakça

  • Aliyazicioglu, Y., Demir, S., Turan, I., Cakiroglu, T., Akalin, I., Deger, O., & Bedir, A. (2011). Preventive and protective effects of Turkish propolis on H 2 O 2 -induced DNA damage in foreskin fibroblast cell lines. Acta Biologica Hungarica, 62(4), 388-396. https://doi.org/10.1556/ABiol.62.2011.4.5
  • Barlak, Y., Değer, O., Çolak, M., Karataylı, S., Bozdayı, A., Yücesan, F. (2011). Effect of Turkish propolis extracts on proteome of prostate cancer cell line. Proteome Science, 9(1), 74. https://doi.org/10.1186/1477-5956-9-74
  • Barlak, Y., Değer, O., Uçar, M., Çakıroğlu, T. N. (2015). Effects of Turkish propolis extract on secretion of polymorphonuclear elastase following respiratory burst. Turkish Journal of Biology, 39, 194-201. https://doi.org/10.3906/biy-1402-48
  • Bozkuş, T. N., Değer, O., Yaşar, A. (2021). Chemical characterization of water and ethanolic extracts of Turkish propolis by HPLC-DAD and GC-MS. Journal of Liquid Chromatography & Related Technologies, 44(1–2), 77-86. https://doi.org/10.1080/10826076.2021.1883648
  • Búfalo, M.C., Candeias, J.M.G., Sousa, J.P.B., Bastos, J.K., Sforcin, J.M. (2010). In vitro cytotoxic activity of Baccharis dracunculifolia and propolis against HEp-2 cells. Natural Product Research, 24(18), 1710-1718. https://doi.org/10.1080/14786410902909300
  • Búfalo, Michelle C., Candeias, J.M.G., Sforcin, J.M. (2009). In Vitro Cytotoxic Effect of Brazilian Green Propolis on Human Laryngeal Epidermoid Carcinoma (HEp-2) Cells. Evidence-Based Complementary and Alternative Medicine, 6(4), 483-487. https://doi.org/10.1093/ecam/nem147
  • da Silva Frozza, C.O., Garcia, C.S.C., Gambato, G., de Souza, M.D.O., Salvador, M., Moura, S., Padilha, F.F., Seixas, F.K., Collares, T., Borsuk, S., Dellagostin, O.A., Henriques, J.A.P., Roesch-Ely, M. (2013). Chemical characterization, antioxidant and cytotoxic activities of Brazilian red propolis. Food and Chemical Toxicology, 52, 137-142. https://doi.org/10.1016/j.fct.2012.11.013
  • Díaz-Carballo, D., Malak, S., Bardenheuer, W., Freistuehler, M., Peter Reusch, H. (2008). The contribution of plukenetione A to the anti-tumoral activity of Cuban propolis. Bioorganic & Medicinal Chemistry, 16(22), 9635-9643. https://doi.org/10.1016/j.bmc.2008.10.019
  • Lee, Y.-T., Don, M.-J., Hung, P.-S., Shen, Y.-C., Lo, Y.-S., Chang, K.-W., Chen, C.-F., Ho, L.-K. (2005). Cytotoxicity of phenolic acid phenethyl esters on oral cancer cells. Cancer Letters, 223(1), 19-25. https://doi.org/10.1016/j.canlet.2004.09.048
  • Mishima, S., Inoh, Y., Narita, Y., Ohta, S., Sakamoto, T., Araki, Y., Suzuki, K.-M., Akao, Y., Nozawa, Y. (2005a). Identification of caffeoylquinic acid derivatives from Brazilian propolis as constituents involved in induction of granulocytic differentiation of HL-60 cells. Bioorganic & Medicinal Chemistry, 13(20), 5814-5818. https://doi.org/10.1016/j.bmc.2005.05.044
  • Mishima, S., Narita, Y., Chikamatsu, S., Inoh, Y., Ohta, S., Yoshida, C., Araki, Y., Akao, Y., Suzuki, K.-M., Nozawa, Y. (2005b). Effects of propolis on cell growth and gene expression in HL-60 cells. Journal of Ethnopharmacology, 99(1), 5-11. https://doi.org/10.1016/j.jep.2005.02.005
  • Nagai, T., Inoue, R., Inoue, H., Suzuki, N. (2003). Preparation and antioxidant properties of water extract of propolis. Food Chemistry, 80(1), 29-33. https://doi.org/10.1016/S0308-8146(02)00231-5
  • Nakajima, Y., Tsuruma, K., Shimazawa, M., Mishima, S., Hara, H. (2009). Comparison of bee products based on assays of antioxidant capacities. BMC Complementary and Alternative Medicine, 9(1), 4. https://doi.org/10.1186/1472-6882-9-4
  • Oršolić, N., Knežević, A.H., Šver, L., Terzić, S., Bašić, I. (2004). Immunomodulatory and antimetastatic action of propolis and related polyphenolic compounds. Journal of Ethnopharmacology, 94(2-3), 307-315. https://doi.org/10.1016/j.jep.2004.06.006
  • Oršolic, N., Šver, L., Terzić, S., Bašić, I. (2005). Peroral Application of Water-soluble Derivative of Propolis (WSDP) and Its Related Polyphenolic Compounds and Their Influence on Immunological and Antitumour Activity. Veterinary Research Communications, 29(7), 575-593. https://doi.org/10.1007/s11259-005-3303-z
  • Russo, A., Cardile, V., Sanchez, F., Troncoso, N., Vanella, A., Garbarino, J.A. (2004). Chilean propolis: antioxidant activity and antiproliferative action in human tumor cell lines. Life Sciences, 76(5), 545–558. https://doi.org/10.1016/j.lfs.2004.07.019
  • Sforcin, J.M. (2007). Propolis and the immune system: a review. Journal of Ethnopharmacology, 113(1), 1-14. https://doi.org/10.1016/j.jep.2007.05.012
  • Sforcin, José Maurício, Bankova, V. (2011). Propolis: Is there a potential for the development of new drugs? Journal of Ethnopharmacology, 133(2), 253-260. https://doi.org/10.1016/j.jep.2010.10.032
  • Suzuki, I., Hayashi, I., Takaki, T., Groveman, D. S., Fujimiya, Y. (2002). Antitumor and Anticytopenic Effects of Aqueous Extracts of Propolis in Combination with Chemotherapeutic Agents. Cancer Biotherapy and Radiopharmaceuticals, 17(5), 553-562. https://doi.org/10.1089/108497802760804781
  • Uçar, M., Değer, O., Barlak, Y. (2016). Effect of Turkish propolis extracts on expression of voltage-gated sodium channel Nav 1.5 and 1.7 α-Isoforms in PC-3 human prostate cancer cells. Tropical Journal of Pharmaceutical Research, 15(10), 2093. https://doi.org/10.4314/tjpr.v15i10.6

Yıl 2022, Cilt 8, Sayı 3, 218 - 228, 01.07.2022
https://doi.org/10.3153/FH22021

Öz

Kaynakça

  • Aliyazicioglu, Y., Demir, S., Turan, I., Cakiroglu, T., Akalin, I., Deger, O., & Bedir, A. (2011). Preventive and protective effects of Turkish propolis on H 2 O 2 -induced DNA damage in foreskin fibroblast cell lines. Acta Biologica Hungarica, 62(4), 388-396. https://doi.org/10.1556/ABiol.62.2011.4.5
  • Barlak, Y., Değer, O., Çolak, M., Karataylı, S., Bozdayı, A., Yücesan, F. (2011). Effect of Turkish propolis extracts on proteome of prostate cancer cell line. Proteome Science, 9(1), 74. https://doi.org/10.1186/1477-5956-9-74
  • Barlak, Y., Değer, O., Uçar, M., Çakıroğlu, T. N. (2015). Effects of Turkish propolis extract on secretion of polymorphonuclear elastase following respiratory burst. Turkish Journal of Biology, 39, 194-201. https://doi.org/10.3906/biy-1402-48
  • Bozkuş, T. N., Değer, O., Yaşar, A. (2021). Chemical characterization of water and ethanolic extracts of Turkish propolis by HPLC-DAD and GC-MS. Journal of Liquid Chromatography & Related Technologies, 44(1–2), 77-86. https://doi.org/10.1080/10826076.2021.1883648
  • Búfalo, M.C., Candeias, J.M.G., Sousa, J.P.B., Bastos, J.K., Sforcin, J.M. (2010). In vitro cytotoxic activity of Baccharis dracunculifolia and propolis against HEp-2 cells. Natural Product Research, 24(18), 1710-1718. https://doi.org/10.1080/14786410902909300
  • Búfalo, Michelle C., Candeias, J.M.G., Sforcin, J.M. (2009). In Vitro Cytotoxic Effect of Brazilian Green Propolis on Human Laryngeal Epidermoid Carcinoma (HEp-2) Cells. Evidence-Based Complementary and Alternative Medicine, 6(4), 483-487. https://doi.org/10.1093/ecam/nem147
  • da Silva Frozza, C.O., Garcia, C.S.C., Gambato, G., de Souza, M.D.O., Salvador, M., Moura, S., Padilha, F.F., Seixas, F.K., Collares, T., Borsuk, S., Dellagostin, O.A., Henriques, J.A.P., Roesch-Ely, M. (2013). Chemical characterization, antioxidant and cytotoxic activities of Brazilian red propolis. Food and Chemical Toxicology, 52, 137-142. https://doi.org/10.1016/j.fct.2012.11.013
  • Díaz-Carballo, D., Malak, S., Bardenheuer, W., Freistuehler, M., Peter Reusch, H. (2008). The contribution of plukenetione A to the anti-tumoral activity of Cuban propolis. Bioorganic & Medicinal Chemistry, 16(22), 9635-9643. https://doi.org/10.1016/j.bmc.2008.10.019
  • Lee, Y.-T., Don, M.-J., Hung, P.-S., Shen, Y.-C., Lo, Y.-S., Chang, K.-W., Chen, C.-F., Ho, L.-K. (2005). Cytotoxicity of phenolic acid phenethyl esters on oral cancer cells. Cancer Letters, 223(1), 19-25. https://doi.org/10.1016/j.canlet.2004.09.048
  • Mishima, S., Inoh, Y., Narita, Y., Ohta, S., Sakamoto, T., Araki, Y., Suzuki, K.-M., Akao, Y., Nozawa, Y. (2005a). Identification of caffeoylquinic acid derivatives from Brazilian propolis as constituents involved in induction of granulocytic differentiation of HL-60 cells. Bioorganic & Medicinal Chemistry, 13(20), 5814-5818. https://doi.org/10.1016/j.bmc.2005.05.044
  • Mishima, S., Narita, Y., Chikamatsu, S., Inoh, Y., Ohta, S., Yoshida, C., Araki, Y., Akao, Y., Suzuki, K.-M., Nozawa, Y. (2005b). Effects of propolis on cell growth and gene expression in HL-60 cells. Journal of Ethnopharmacology, 99(1), 5-11. https://doi.org/10.1016/j.jep.2005.02.005
  • Nagai, T., Inoue, R., Inoue, H., Suzuki, N. (2003). Preparation and antioxidant properties of water extract of propolis. Food Chemistry, 80(1), 29-33. https://doi.org/10.1016/S0308-8146(02)00231-5
  • Nakajima, Y., Tsuruma, K., Shimazawa, M., Mishima, S., Hara, H. (2009). Comparison of bee products based on assays of antioxidant capacities. BMC Complementary and Alternative Medicine, 9(1), 4. https://doi.org/10.1186/1472-6882-9-4
  • Oršolić, N., Knežević, A.H., Šver, L., Terzić, S., Bašić, I. (2004). Immunomodulatory and antimetastatic action of propolis and related polyphenolic compounds. Journal of Ethnopharmacology, 94(2-3), 307-315. https://doi.org/10.1016/j.jep.2004.06.006
  • Oršolic, N., Šver, L., Terzić, S., Bašić, I. (2005). Peroral Application of Water-soluble Derivative of Propolis (WSDP) and Its Related Polyphenolic Compounds and Their Influence on Immunological and Antitumour Activity. Veterinary Research Communications, 29(7), 575-593. https://doi.org/10.1007/s11259-005-3303-z
  • Russo, A., Cardile, V., Sanchez, F., Troncoso, N., Vanella, A., Garbarino, J.A. (2004). Chilean propolis: antioxidant activity and antiproliferative action in human tumor cell lines. Life Sciences, 76(5), 545–558. https://doi.org/10.1016/j.lfs.2004.07.019
  • Sforcin, J.M. (2007). Propolis and the immune system: a review. Journal of Ethnopharmacology, 113(1), 1-14. https://doi.org/10.1016/j.jep.2007.05.012
  • Sforcin, José Maurício, Bankova, V. (2011). Propolis: Is there a potential for the development of new drugs? Journal of Ethnopharmacology, 133(2), 253-260. https://doi.org/10.1016/j.jep.2010.10.032
  • Suzuki, I., Hayashi, I., Takaki, T., Groveman, D. S., Fujimiya, Y. (2002). Antitumor and Anticytopenic Effects of Aqueous Extracts of Propolis in Combination with Chemotherapeutic Agents. Cancer Biotherapy and Radiopharmaceuticals, 17(5), 553-562. https://doi.org/10.1089/108497802760804781
  • Uçar, M., Değer, O., Barlak, Y. (2016). Effect of Turkish propolis extracts on expression of voltage-gated sodium channel Nav 1.5 and 1.7 α-Isoforms in PC-3 human prostate cancer cells. Tropical Journal of Pharmaceutical Research, 15(10), 2093. https://doi.org/10.4314/tjpr.v15i10.6

Ayrıntılar

Birincil Dil İngilizce
Konular Tıp
Bölüm Research Articles
Yazarlar

Betül DEĞER KULAKSIZ> (Sorumlu Yazar)
SAĞLIK BİLİMLERİ ÜNİVERSİTESİ, TRABZON KANUNİ SAĞLIK UYGULAMA VE ARAŞTIRMA MERKEZİ, CERRAHİ TIP BİLİMLERİ BÖLÜMÜ
0000-0002-5486-6139
Türkiye


Mehmet İMAMOĞLU Bu kişi benim
KARADENİZ TEKNİK ÜNİVERSİTESİ, TIP FAKÜLTESİ
0000-0001-7259-9567
Türkiye


Orhan DEĞER>
KARADENİZ TEKNİK ÜNİVERSİTESİ, TIP FAKÜLTESİ
0000-0003-3584-6324
Türkiye


Kübra AKBULUT ÇAKIROĞLU Bu kişi benim
KARADENİZ TEKNİK ÜNİVERSİTESİ, TIP FAKÜLTESİ
0000-0003-0227-5174
Türkiye


Selim DEMİR>
KARADENIZ TECHNICAL UNIVERSITY, INSTITUTE OF HEALTH SCIENCES
0000-0002-1863-6280
Türkiye


Mehmet SÖNMEZ Bu kişi benim
KARADENİZ TEKNİK ÜNİVERSİTESİ, TIP FAKÜLTESİ
0000-0002-2176-4371
Türkiye

Destekleyen Kurum The study was supported by Karadeniz Technical University Research Funding
Proje Numarası 10010
Yayımlanma Tarihi 1 Temmuz 2022
Başvuru Tarihi 12 Aralık 2021
Kabul Tarihi 14 Mart 2022
Yayınlandığı Sayı Yıl 2022, Cilt 8, Sayı 3

Kaynak Göster

Bibtex @araştırma makalesi { jfhs1023344, journal = {Food and Health}, eissn = {2602-2834}, address = {Vidin Caddesi No:28 D:4 Kocamustafapaşa 34107 Fatih İstanbul}, publisher = {Özkan ÖZDEN}, year = {2022}, volume = {8}, number = {3}, pages = {218 - 228}, doi = {10.3153/FH22021}, title = {Cytotoxic effects of water and ethanolic extracts of Turkish propolis on human laryngeal epidermoid carcinoma cell lines}, key = {cite}, author = {Değer Kulaksız, Betül and İmamoğlu, Mehmet and Değer, Orhan and Akbulut Çakıroğlu, Kübra and Demir, Selim and Sönmez, Mehmet} }
APA Değer Kulaksız, B. , İmamoğlu, M. , Değer, O. , Akbulut Çakıroğlu, K. , Demir, S. & Sönmez, M. (2022). Cytotoxic effects of water and ethanolic extracts of Turkish propolis on human laryngeal epidermoid carcinoma cell lines . Food and Health , 8 (3) , 218-228 . DOI: 10.3153/FH22021
MLA Değer Kulaksız, B. , İmamoğlu, M. , Değer, O. , Akbulut Çakıroğlu, K. , Demir, S. , Sönmez, M. "Cytotoxic effects of water and ethanolic extracts of Turkish propolis on human laryngeal epidermoid carcinoma cell lines" . Food and Health 8 (2022 ): 218-228 <http://jfhs.scientificwebjournals.com/tr/pub/issue/69254/1023344>
Chicago Değer Kulaksız, B. , İmamoğlu, M. , Değer, O. , Akbulut Çakıroğlu, K. , Demir, S. , Sönmez, M. "Cytotoxic effects of water and ethanolic extracts of Turkish propolis on human laryngeal epidermoid carcinoma cell lines". Food and Health 8 (2022 ): 218-228
RIS TY - JOUR T1 - Cytotoxic effects of water and ethanolic extracts of Turkish propolis on human laryngeal epidermoid carcinoma cell lines AU - BetülDeğer Kulaksız, Mehmetİmamoğlu, OrhanDeğer, KübraAkbulut Çakıroğlu, SelimDemir, MehmetSönmez Y1 - 2022 PY - 2022 N1 - doi: 10.3153/FH22021 DO - 10.3153/FH22021 T2 - Food and Health JF - Journal JO - JOR SP - 218 EP - 228 VL - 8 IS - 3 SN - -2602-2834 M3 - doi: 10.3153/FH22021 UR - https://doi.org/10.3153/FH22021 Y2 - 2022 ER -
EndNote %0 Food and Health Cytotoxic effects of water and ethanolic extracts of Turkish propolis on human laryngeal epidermoid carcinoma cell lines %A Betül Değer Kulaksız , Mehmet İmamoğlu , Orhan Değer , Kübra Akbulut Çakıroğlu , Selim Demir , Mehmet Sönmez %T Cytotoxic effects of water and ethanolic extracts of Turkish propolis on human laryngeal epidermoid carcinoma cell lines %D 2022 %J Food and Health %P -2602-2834 %V 8 %N 3 %R doi: 10.3153/FH22021 %U 10.3153/FH22021
ISNAD Değer Kulaksız, Betül , İmamoğlu, Mehmet , Değer, Orhan , Akbulut Çakıroğlu, Kübra , Demir, Selim , Sönmez, Mehmet . "Cytotoxic effects of water and ethanolic extracts of Turkish propolis on human laryngeal epidermoid carcinoma cell lines". Food and Health 8 / 3 (Temmuz 2022): 218-228 . https://doi.org/10.3153/FH22021
AMA Değer Kulaksız B. , İmamoğlu M. , Değer O. , Akbulut Çakıroğlu K. , Demir S. , Sönmez M. Cytotoxic effects of water and ethanolic extracts of Turkish propolis on human laryngeal epidermoid carcinoma cell lines. Food Health. 2022; 8(3): 218-228.
Vancouver Değer Kulaksız B. , İmamoğlu M. , Değer O. , Akbulut Çakıroğlu K. , Demir S. , Sönmez M. Cytotoxic effects of water and ethanolic extracts of Turkish propolis on human laryngeal epidermoid carcinoma cell lines. Food and Health. 2022; 8(3): 218-228.
IEEE B. Değer Kulaksız , M. İmamoğlu , O. Değer , K. Akbulut Çakıroğlu , S. Demir ve M. Sönmez , "Cytotoxic effects of water and ethanolic extracts of Turkish propolis on human laryngeal epidermoid carcinoma cell lines", Food and Health, c. 8, sayı. 3, ss. 218-228, Tem. 2022, doi:10.3153/FH22021

Food and Health" journal is licensed under a CreativeCommons Attribtion-ShareAlike 4.0 International Licence 14627 13310

openaccess.jpgOpen Access Statement:

This is an open access journal which means that all content is freely available without charge to the user or his/her institution. Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles, or use them for any other lawful purpose, without asking prior permission from the publisher or the author. This is in accordance with the BOAI definition of open access.