Research Article
BibTex RIS Cite

Health effects of ethanolic extract from seeds of lady finger

Year 2020, , 90 - 97, 01.04.2020

Jasadee Kaewsrichan , Paweena Wongwitwichot Sirikhwan Manee

https://doi.org/10.3153/FH20010

Abstract

Health benefits of lady finger were explored in this study by which the ethanol extract from the seeds was utilized for testing. In high glucose (350 mmole/L) medium, the proliferative activity of C2C12 cells was inferior, but their viability was improved following treated with the extract. The activities of antioxidant enzymes, superoxide dismutase (SOD) and catalase (CAT), were also enhanced. In response to such high glucose concentration, glucose uptake ability of these cells was compromised. The extract was demonstrated to increase the ability of glucose uptake by approximately equal to the effect of 200 nmole/L insulin. High intracellular reactive oxygen species (ROS) of RBM-MCS cells grown in high glucose containing medium was elicited. The level of ROS was reduced when the cells were cultured in contact with the extract. L929 cells were made inflammation and oxidative stress by incubating with 37.5 µmole/L DPPH for 30 min. The release of tumor necrosis factor-α (TNF-α) and interleukin 6 (IL-6) from these inflamed cells was inhibited by such treatment. Thus, lady finger might be advantageous in the protection of cells in the body that would be damaged or being susceptible to hyperglycemia and diabetes.

Keywords

Lady finger Polyphenols Healthy diet Nutrient Obesity Chronic inflammation Metabolic disease

Supporting Institution

The financial support of this project was granted by Thailand national budget for the fiscal year 2018 under integrative research and innovative plan.

Project Number

PHA610372S

References

  • Alam, P., Parvez, M.K., Arbab, A.H., Siddiqui, N.A., Al-Dosary, M.S., Al-Rehaily, A.J., Ahmed, S., Kalam, M.A. Ahmad, M.S. (2017). Inter-species comparative antioxidant assay and HPTLC analysis of sakuranetin in the chloroform and ethanol extracts of aerial parts of Rhus retinorrhoea and Rhus tripartita. Pharmaceutical Biology, 55(1), 1450-1457. https://doi.org/10.1080/13880209.2017.1304428
  • Azlim Almey, A., Ahmed Jalal Khan, C., Syed Zahir, I., Mustapha Suleiman, K., Aisyah, M. Kamarul Rahim, K. (2010). Total phenolic content and primary antioxidant activity of methanolic and ethanolic extracts of aromatic plants' leaves. International Food Research Journal, 17(4), 1077-1084.
  • Bianco, P., Robey, P.G. Simmons, P.J. (2008). Mesenchymal stem cells: revisiting history, concepts, and assays. Cell stem Cell, 2(4), 313-319. https://doi.org/10.1016/j.stem.2008.03.002
  • Castro, A., Macedo-de la Concha, L. Pantoja-Meléndez, C. (2017). Low-grade inflammation and its relation to obesity and chronic degenerative diseases. Revista Médica del Hospital General de México, 80(2), 101-105. https://doi.org/10.1016/j.hgmx.2016.06.011
  • Dubois, V., Eeckhoute, J., Lefebvre, P. Staels, B. (2017). Distinct but complementary contributions of PPAR isotypes to energy homeostasis. The Journal of clinical investigation, 127(4), 1202-1214. https://doi.org/10.1172/JCI88894
  • Giustarini, D., Dalle-Donne, I., Tsikas, D. Rossi, R. (2009). Oxidative stress and human diseases: origin, link, measurement, mechanisms, and biomarkers. Critical Reviews in Clinical Laboratory Sciences, 46(5-6), 241-281. https://doi.org/10.3109/10408360903142326
  • Healthy diet. (2018, October 23). Retrieved from https://www.who.int/news-room/fact-sheets/detail/healthy-diet (accessed 18.08.2019).
  • A healthy, balanced diet. (2016, October). Retrieved from https://www.nutrition.org.uk/healthyliving/healthydiet/healthybalanceddiet.html (accessed 18.08.2019).
  • Ighodaro, O. Akinloye, O. (2018). First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): Their fundamental role in the entire antioxidant defence grid. Alexandria Journal of Medicine, 54(4), 287-293. https://doi.org/10.1016/j.ajme.2017.09.001
  • Kant, V., Gopal, A., Pathak, N.N., Kumar, P., Tandan, S.K. Kumar, D. (2014). Antioxidant and anti-inflammatory potential of curcumin accelerated the cutaneous wound healing in streptozotocin-induced diabetic rats. International Immunopharmacology, 20(2), 322-330. https://doi.org/10.1016/j.intimp.2014.03.009
  • Kim, Y. Park, C.W. (2019). Mechanisms of adiponectin action: Implication of adiponectin receptor agonism in diabetic Kidney disease. International Journal of Molecular Sciences, 20(7), 1782. https://doi.org/10.3390/ijms20071782
  • Lucassen, E.A. Cizza, G. (2012). The hypothalamic-pituitary-adrenal axis, obesity, and chronic stress exposure: sleep and the HPA axis in obesity. Current Obesity Reports, 1(4), 208-215. https://doi.org/10.1007/s13679-012-0028-5 Manee, S. Kaewsrichan, J. (2017). Cosmeceutical benefit of Abelmoschus esculentus L. seed extract. Journal of Pharmaceutical Research International, 19(6), 1-11. https://doi.org/10.9734/JPRI/2017/37587
  • Marcovecchio, M.L. (2017). Complications of acute and chronic hyperglycemia. US Endocrinology, 13, 1-17. https://doi.org/10.17925/USE.2017.13.01.17
  • Newsholme, P., Haber, E.P., Hirabara, S.M., Rebelato, E.L.O., Procopio, J., Morgan, D., Oliveira-Emilio, H.C., Carpinelli, A.R. Curi, R. (2007). Diabetes associated cell stress and dysfunction: role of mitochondrial and non-mitochondrial ROS production and activity. The Journal of Physiology, 583(1), 9-24. https://doi.org/10.1113/jphysiol.2007.135871
  • Rastogi, R.P., Singh, S.P., Häder, D.-P. Sinha, R.P. (2010). Detection of reactive oxygen species (ROS) by the oxidant-sensing probe 2′,7′-dichlorodihydrofluorescein diacetate in the cyanobacterium Anabaena variabilis PCC 7937. Biochemical and Biophysical Research Communications, 397(3), 603-607. https://doi.org/10.1016/j.bbrc.2010.06.006
  • Rehakova, Z., Koleckar, V., Jahodar, L., Opletal, L., Macakova, K., Cahlikova, L., Jun, D. Kuca, K. (2014). Evaluation of the antioxidant activity of several naturally oc curring coumarins and their synthesized analogues by "ferric reducing antioxidant power" assay. Journal of Enzyme Inhibition and Medicinal Chemistry, 29(1), 49-54. https://doi.org/10.3109/14756366.2012.753589
  • Shannon, C., Merovci, A., Tripathy, D., Abdul-Ghani, M., Norton, L. Defronzo, R.A. (2018). Effects of hyperglycemia on skeletal muscle glucose metabolism in healthy subjects. Diabetes, 67(Supplement 1), 1920-P. https://doi.org/10.2337/db18-1920-P
  • Ware, M. (2019). Benefits and uses of okra. Retrieved from https://www.medicalnewstoday.com/articles/311977.php (accessed 15.11.2019)
  • Wongwitwichot, P., Kaewsrichan, J. (2017). Induction of rat marrow stromal cells by FGF2 and insulin activates transcription of runx2 gene through ap1 consensus sequence. Journal of Advances in Medicine and Medical Research, 24(7), 1-15. https://doi.org/10.9734/JAMMR/2017/37534
  • Woodfield, J. (2016, March 03). Study uncovers extent of muscle weakness in people with type 2 diabetes. Retrieved from https://www.diabetes.co.uk/news/2016/mar/study-uncovers-extent-of-muscle-weakness-in-people-with-type-2-diabetes-92465800.html (accessed 18.08.2019).

Year 2020, , 90 - 97, 01.04.2020

Jasadee Kaewsrichan , Paweena Wongwitwichot Sirikhwan Manee

https://doi.org/10.3153/FH20010

Abstract

Project Number

PHA610372S

References

  • Alam, P., Parvez, M.K., Arbab, A.H., Siddiqui, N.A., Al-Dosary, M.S., Al-Rehaily, A.J., Ahmed, S., Kalam, M.A. Ahmad, M.S. (2017). Inter-species comparative antioxidant assay and HPTLC analysis of sakuranetin in the chloroform and ethanol extracts of aerial parts of Rhus retinorrhoea and Rhus tripartita. Pharmaceutical Biology, 55(1), 1450-1457. https://doi.org/10.1080/13880209.2017.1304428
  • Azlim Almey, A., Ahmed Jalal Khan, C., Syed Zahir, I., Mustapha Suleiman, K., Aisyah, M. Kamarul Rahim, K. (2010). Total phenolic content and primary antioxidant activity of methanolic and ethanolic extracts of aromatic plants' leaves. International Food Research Journal, 17(4), 1077-1084.
  • Bianco, P., Robey, P.G. Simmons, P.J. (2008). Mesenchymal stem cells: revisiting history, concepts, and assays. Cell stem Cell, 2(4), 313-319. https://doi.org/10.1016/j.stem.2008.03.002
  • Castro, A., Macedo-de la Concha, L. Pantoja-Meléndez, C. (2017). Low-grade inflammation and its relation to obesity and chronic degenerative diseases. Revista Médica del Hospital General de México, 80(2), 101-105. https://doi.org/10.1016/j.hgmx.2016.06.011
  • Dubois, V., Eeckhoute, J., Lefebvre, P. Staels, B. (2017). Distinct but complementary contributions of PPAR isotypes to energy homeostasis. The Journal of clinical investigation, 127(4), 1202-1214. https://doi.org/10.1172/JCI88894
  • Giustarini, D., Dalle-Donne, I., Tsikas, D. Rossi, R. (2009). Oxidative stress and human diseases: origin, link, measurement, mechanisms, and biomarkers. Critical Reviews in Clinical Laboratory Sciences, 46(5-6), 241-281. https://doi.org/10.3109/10408360903142326
  • Healthy diet. (2018, October 23). Retrieved from https://www.who.int/news-room/fact-sheets/detail/healthy-diet (accessed 18.08.2019).
  • A healthy, balanced diet. (2016, October). Retrieved from https://www.nutrition.org.uk/healthyliving/healthydiet/healthybalanceddiet.html (accessed 18.08.2019).
  • Ighodaro, O. Akinloye, O. (2018). First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): Their fundamental role in the entire antioxidant defence grid. Alexandria Journal of Medicine, 54(4), 287-293. https://doi.org/10.1016/j.ajme.2017.09.001
  • Kant, V., Gopal, A., Pathak, N.N., Kumar, P., Tandan, S.K. Kumar, D. (2014). Antioxidant and anti-inflammatory potential of curcumin accelerated the cutaneous wound healing in streptozotocin-induced diabetic rats. International Immunopharmacology, 20(2), 322-330. https://doi.org/10.1016/j.intimp.2014.03.009
  • Kim, Y. Park, C.W. (2019). Mechanisms of adiponectin action: Implication of adiponectin receptor agonism in diabetic Kidney disease. International Journal of Molecular Sciences, 20(7), 1782. https://doi.org/10.3390/ijms20071782
  • Lucassen, E.A. Cizza, G. (2012). The hypothalamic-pituitary-adrenal axis, obesity, and chronic stress exposure: sleep and the HPA axis in obesity. Current Obesity Reports, 1(4), 208-215. https://doi.org/10.1007/s13679-012-0028-5 Manee, S. Kaewsrichan, J. (2017). Cosmeceutical benefit of Abelmoschus esculentus L. seed extract. Journal of Pharmaceutical Research International, 19(6), 1-11. https://doi.org/10.9734/JPRI/2017/37587
  • Marcovecchio, M.L. (2017). Complications of acute and chronic hyperglycemia. US Endocrinology, 13, 1-17. https://doi.org/10.17925/USE.2017.13.01.17
  • Newsholme, P., Haber, E.P., Hirabara, S.M., Rebelato, E.L.O., Procopio, J., Morgan, D., Oliveira-Emilio, H.C., Carpinelli, A.R. Curi, R. (2007). Diabetes associated cell stress and dysfunction: role of mitochondrial and non-mitochondrial ROS production and activity. The Journal of Physiology, 583(1), 9-24. https://doi.org/10.1113/jphysiol.2007.135871
  • Rastogi, R.P., Singh, S.P., Häder, D.-P. Sinha, R.P. (2010). Detection of reactive oxygen species (ROS) by the oxidant-sensing probe 2′,7′-dichlorodihydrofluorescein diacetate in the cyanobacterium Anabaena variabilis PCC 7937. Biochemical and Biophysical Research Communications, 397(3), 603-607. https://doi.org/10.1016/j.bbrc.2010.06.006
  • Rehakova, Z., Koleckar, V., Jahodar, L., Opletal, L., Macakova, K., Cahlikova, L., Jun, D. Kuca, K. (2014). Evaluation of the antioxidant activity of several naturally oc curring coumarins and their synthesized analogues by "ferric reducing antioxidant power" assay. Journal of Enzyme Inhibition and Medicinal Chemistry, 29(1), 49-54. https://doi.org/10.3109/14756366.2012.753589
  • Shannon, C., Merovci, A., Tripathy, D., Abdul-Ghani, M., Norton, L. Defronzo, R.A. (2018). Effects of hyperglycemia on skeletal muscle glucose metabolism in healthy subjects. Diabetes, 67(Supplement 1), 1920-P. https://doi.org/10.2337/db18-1920-P
  • Ware, M. (2019). Benefits and uses of okra. Retrieved from https://www.medicalnewstoday.com/articles/311977.php (accessed 15.11.2019)
  • Wongwitwichot, P., Kaewsrichan, J. (2017). Induction of rat marrow stromal cells by FGF2 and insulin activates transcription of runx2 gene through ap1 consensus sequence. Journal of Advances in Medicine and Medical Research, 24(7), 1-15. https://doi.org/10.9734/JAMMR/2017/37534
  • Woodfield, J. (2016, March 03). Study uncovers extent of muscle weakness in people with type 2 diabetes. Retrieved from https://www.diabetes.co.uk/news/2016/mar/study-uncovers-extent-of-muscle-weakness-in-people-with-type-2-diabetes-92465800.html (accessed 18.08.2019).
There are 20 citations in total.

Details

Primary Language English
Subjects Clinical Sciences
Journal Section Research Articles
Authors

Jasadee Kaewsrichan 0000-0003-4316-3814

Paweena Wongwitwichot 0000-0002-5112-0913

Sirikhwan Manee 0000-0003-2958-4284

Project Number PHA610372S
Publication Date April 1, 2020
Submission Date September 25, 2019
Published in Issue Year 2020

Cite

APA Kaewsrichan, J., Wongwitwichot, P., & Manee, S. (2020). Health effects of ethanolic extract from seeds of lady finger. Food and Health, 6(2), 90-97. https://doi.org/10.3153/FH20010

ustresim_2021_1.png

Journal is licensed under a 

CreativeCommons Attribtion-ShareAlike 4.0 International Licence 146271331027042

Diamond Open Access refers to a scholarly publication model in which journals and platforms do not charge fees to either authors or readers.

Open Access Statement:
This is an open access journal which means that all content is freely available without charge to the user or his/her institution. Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles, or use them for any other lawful purpose, without asking prior permission from the publisher or the author. This is in accordance with the BOAI definition of open access.

Archiving Policy:


Archiving is done according to ULAKBİM "DergiPark" publication policy (LOCKSS).