In vitro trypsin inhibitory activities of some plant and fruit extracts and chemical compounds

Ebru İlhan Lale; İsmet Burcu Turkyilmaz; Refiye Yanardağ

doi:10.3153/FH23025

Research Article

In vitro trypsin inhibitory activities of some plant and fruit extracts and chemical compounds

Year 2023, , 282 - 292, 08.10.2023

Ebru İlhan Lale , İsmet Burcu Turkyilmaz , Refiye Yanardağ

https://doi.org/10.3153/FH23025

Abstract

Trypsin, an enzyme from the serine protease class, is known to be involved in the degradation of proteins. Excessive activity of trypsin is strongly implicated in triggering many diseases, such as acute pancreatitis, inflammation, and tumour. Therefore, this enzyme's regular and balanced activity is necessary for normal physiological functions. Thus, there is a need to develop new trypsin inhibitors from natural sources and chemical compounds. In this study, the inhibitory effects of aqueous extracts prepared from 29 different plants and 10 different chemical compounds were investigated on the activity of trypsin due to its importance in the health sector. The present study's plant extracts and chemical compounds showed trypsin-inhibitory effects. The inhibitory activities of the extracts and chemical compounds increased in a dose-dependent manner. Several plant extracts and chemical compounds that showed high trypsin inhibitory activities may be appropriate for use as trypsin inhibitors to provide additional support to drug treatment in the health field.

Keywords

Trypsin, Plant extract, Enzyme inhibition, Chemical compounds

Supporting Institution

Scientific Research Projects Coordination Unit of Istanbul University

Project Number

41104

References

Abd el-Rady, N.M., Dahpy, M.A., Ahmed, A., Elgamal, D.A., Hadiya, S., Ahmed, M.A.M., Sayed, Z.E.A.A., Abdeltawab, D., Abdelmohsen, A.S., Farrag, A.A.M., Ashmawy, A.M., Khairallah, M.K., Galal, H.M. (2021). Interplay of biochemical, genetic, and immunohistochemical factors in the etio-pathogenesis of gastric ulcer in rats: A comparative study of the effect of pomegranate loaded nanoparticles versus pomegranate peel extract. Frontiers in Physiology, 12 (649462). https://doi.org/10.3389/fphys.2021.649462
Abdellatif, F., Boudjella, H., Zitouni, A., Hassani, A. (2014). Chemical composition and antimicrobial activity of the essential oil from leaves of Algerian Melissa officinalis L. EXCLI Journal, 13, 772–781. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4464394/pdf/EXCLI-13-772.pdf
Bateman, K.S., James, M.N.G. (2011). Plant protein proteinase inhibitors: structure and mechanism of inhibition. Current Protein & Peptide Science, 12, 340–347. https://doi.org/10.2174/138920311796391124
Cid-Gallegos, M.S., Corzo-Ríos, L.J., Jiménez-Martínez, C., Sánchez-Chino, X.M. (2022). Protease inhibitors from plants as therapeutic agents - A review. Plant Foods for Human Nutrition, 77, 20–29. https://doi.org/10.1007/s11130-022-00949-4
Cheshomi, H., Bahrami, A.R., Rafatpanah, H., Matin, M.M. (2022). The effects of ellagic acid and other pomegranate (Punica granatum L.) derivatives on human gastric cancer AGS cells. Human and Experimental Toxicology, 41, 1-16. https://doi.org/10.1177/09603271211064534 Clemente, M., Corigliano, M., Pariani, S., Sánchez-López, E., Sander, V., Ramos-Duarte, V. (2019). Plant serine protease inhibitors: Biotechnology application in agriculture and molecular farming. International Journal of Molecular Sciences, 20, 1345. https://doi.org/10.3390/ijms20061345 Dağsuyu, E., Yanardağ, R. (2021). In vitro urease and trypsin inhibitory activities of some sulfur compounds. Istanbul Journal of Pharmacy, 51, 85–91. https://dergipark.org.tr/en/download/article-file/1461510
de Oliveira Braga, L.E., da Silva, G.G., de Oliveira Sousa, I.M., de Oliveira, E.C.S., Jorge, M.P., Monteiro, K.M., Sedano, T.C., Foglio, M.A., Ruiz, A.L.T.G. (2022). Gastrointestinal effects of Mentha aquatica L. essential oil. Inflammopharmacology, 30, 2127-2137. https://doi.org/10.1007/s10787-022-00989-x
Elsässer, B., Goettig, P. (2021). Mechanisms of proteolytic enzymes and their inhibition in QM/MM studies. International Journal of Molecular Sciences, 22, 3232. https://doi.org/10.3390/ijms22063232
Fan, F.Y., Huang, C.S., Tong, Y.L., Guo, H.W., Zhou, S.J., Ye, J.H., Gong, S.Y. (2021). Widely targeted metabolomics analysis of white peony teas with different storage time and association with sensory attributes. Food Chemistry, 362, 130257. https://doi.org/10.1016/j.foodchem.2021.130257
Feng, Y., Lv, M., Lu, Y., Liu, K., Liu, L., He, Z., Wu, K., Wang, X., Zhang, B., Wu, X. (2018). Characterization of binding interactions between selected phenylpropanoid glycosides and trypsin. Food Chemistry, 243, 118–124. https://doi.org/10.1016/j.foodchem.2017.09.118
Geisslitz, S., Weegels, P., Shewry, P., Zevallos, V., Masci, S., Sorrells, M., Gregorini, A., Colomba, M., Jonkers, D., Huang, X., de Giorgio, R., Caio, G.P., D’Amico, S., Larré, C., Brouns, F. (2022). Wheat amylase/trypsin inhibitors (ATIs): occurrence, function and health aspects. European Journal of Nutrition, 61, 2873-2880. https://doi.org/10.1007/s00394-022-02841-y
Ghosh, K.S., Debnath, J., Pathak, T., Dasgupta, S. (2008). Using proton nuclear magnetic resonance to study the mode of ribonuclease A inhibition by competitive and noncompetitive inhibitors. Bioorganic & Medicinal Chemistry Letters, 18, 5503–5506. https://doi.org/10.1016/j.bmcl.2008.09.014
Gräwe, A., Ranglack, J., Weber, W., Stein, V. (2020). Engineering artificial signaling functions with proteases. Current Opinion in Biotechnology, 63, 1–7. https://doi.org/10.1016/j.copbio.2019.09.017
Greene, C.M., McElvaney, N.G. (2009). Proteases and antiproteases in chronic neutrophilic lung disease - relevance to drug discovery. British Journal of Pharmacology, 158, 1048–1058. https://doi.org/10.1111/j.1476-5381.2009.00448.x
Haq, S.K., Atif, S.M., Khan, R.H. (2004). Protein proteinase inhibitor genes in combat against insects, pests, and pathogens: natural and engineered phytoprotection. Archives of Biochemistry and Biophysics, 431, 145–159. https://doi.org/10.1016/j.abb.2004.07.022
Hegyi, E., Sahin-Tóth, M. (2017). Genetic risk in chronic pancreatitis: The trypsin-dependent pathway. Digestive Diseases and Sciences, 62, 1692–1701. https://doi.org/10.1007/s10620-017-4601-3
Hovhannisyan, N., Harutyunyan, Sh., Hovhannisyan, A., Hambardzumyan, A., Chitchyan, M., Melkumyan, M., Oganezova, G., Avetisyan, N. (2009). The novel inhibitors of serine proteases. Amino Acids, 37, 531–536. https://doi.org/10.1007/s00726-009-0257-4
Kan, L., Capuano, E., Fogliano, V., Verkerk, R., Mes, J.J., Tomassen, M.M.M., Oliviero, T. (2021). Inhibition of α-glucosidases by tea polyphenols in rat intestinal extract and Caco-2 cells grown on transwell. Food Chemistry, 361, 130047. https://doi.org/10.1016/j.foodchem.2021.130047
Li, Q., Wei, Q., Yuan, E., Yang, J., Ning, Z. (2014). Interaction between four flavonoids and trypsin: effect on the characteristics of trypsin and antioxidant activity of flavonoids. International Journal of Food Science & Technology, 49, 1063–1069. https://doi.org/10.1111/ijfs.12401
Mancek-Keber, M. (2014). Inflammation-mediating proteases: Structure, function in (patho) physiology and inhibition. Protein and Peptide Letters, 21, 1209–1229. https://doi.org/10.2174/0929866521666140819123932
Mashayekhi-Sardoo, H., Razavi, B.M., Ekhtiari, M., Kheradmand, N., Imenshahidi, M. (2020). Gastroprotective effects of both aqueous and ethanolic extracts of Lemon verbena leaves against indomethacin-induced gastric ulcer in rats. Iranian Journal of Basic Medical Sciences, 23, 1639–1646. https://doi.org/10.22038/ijbms.2020.44341.10377
Navaei-Bonab, R., Kazzazi, M., Saber, M., Vatanparast, M. (2018). Differential inhibition of Helicoverpa armigera (Lep.: Noctuidae) gut digestive trypsin by extracted and purified inhibitor of datura metel (Solanales: Solanaceae). Journal of Economic Entomology, 111, 178–186. https://doi.org/10.1093/jee/tox209
Park, K.C., Dharmasivam, M., Richardson, D.R. (2020). The role of extracellular proteases in tumor progression and the development of innovative metal ion chelators that inhibit their activity. International Journal of Molecular Sciences, 21, 6805. https://doi.org/10.3390/ijms21186805
Regulski, M., Regulska, K., Stanisz, B., Murias, M., Gieremek, P., Wzgarda, A., Niznik, B. (2015). Chemistry and pharmacology of angiotensin-converting enzyme inhibitors. Current Pharmaceutical Design, 21, 1764–1775. https://doi.org/10.2174/1381612820666141112160013
Ribeiro, J.K.C., Cunha, D.D.S., Fook, J.M.S.LL., Sales, M.P. (2010). New properties of the soybean trypsin inhibitor: Inhibition of human neutrophil elastase and its effect on acute pulmonary injury. European Journal of Pharmacology, 644, 238–244. https://doi.org/10.1016/j.ejphar.2010.06.067
Saberi, A., Abbasloo, E., Sepehri, G., Yazdanpanah, M., Mirkamandari, E., Sheibani, V., Safi, Z. (2016). The Effects of methanolic extract of Melissa officinalis on experimental gastric ulcers in rats. Iranian Red Crescent Medical Journal, 18, e24271. https://doi.org/10.5812/ircmj.24271
Sacan, O., Yildiz Turhan, E. (2014). Lipoxygenase inhibitory activities of some plant extracts and chemical compounds. European Journal of Biology, 73, 47–52.
Satoh, H., Amagase, K., Takeuchi, K. (2014). Mucosal protective agents prevent exacerbation of NSAID-induced small intestinal lesions caused by antisecretory drugs in rats. Journal of Pharmacology and Experimental Therapeutics, 348, 227–235. https://doi.org/10.1124/jpet.113.208991
Srikanth, S., Chen, Z. (2016). Plant protease inhibitors in therapeutics-focus on cancer therapy. Frontiers in Pharmacology, 7, 470. https://doi.org/10.3389/fphar.2016.00470
Sultana, M.S., Millwood, R.J., Mazarei, M., Stewart, C.N. (2022). Proteinase inhibitors in legume herbivore defense: From natural to genetically engineered protectants. Plant Cell Reports, 41, 293–305. https://doi.org/10.1007/s00299-021-02800-7
Tarantino, A., Difonzo, G., Lopriore, G., Disciglio, G., Paradiso, V.M., Gambacorta, G., Caponio, F. (2020). Bioactive compounds and quality evaluation of ‘Wonderful’ pomegranate fruit and juice as affected by deficit irrigation. Journal of the Science of Food and Agriculture, 100, 5539–5545. https://doi.org/10.1002/jsfa.10606
Topaloglu, D., Turkyilmaz, I.B., Yanardag, R. (2022). Gastroprotective effect of vitamin U in D-galactosamine-induced hepatotoxicity. Journal of Biochemical and Molecular Toxicology, 36, e23124. https://doi.org/10.1002/jbt.23124
Turkyilmaz, I.B., Coskun, Z.M., Bolkent, S., Yanardag, R. (2019). The effects of antioxidant combination on indomethacin-induced gastric mucosal injury in rats. Cellular and Molecular Biology, 65, 76-83. https://doi.org/10.14715/cmb/2019.65.3.11
Uçan, F., Ağçam, E., Akyildiz, A. (2016). Bioactive compounds and quality parameters of natural cloudy lemon juices. Journal of Food Science and Technology, 53, 1465–1474. https://doi.org/10.1007/s13197-015-2155-y
Viskupicova, J., Danihelova, M., Majekova, M., Liptaj, T., Sturdik, E. (2012). Polyphenol fatty acid esters as serine protease inhibitors: a quantum-chemical QSAR analysis. Journal of Enzyme Inhibition and Medicinal Chemistry, 27, 800–809. https://doi.org/10.3109/14756366.2010.616860
Yi, C., Wu, W., Zheng, D., Peng, G., Huang, H., Shen, Z., Teng, X. (2020). Targeted inhibition of endothelial calpain delays wound healing by reducing inflammation and angiogenesis. Cell Death & Disease, 11, 533. https://doi.org/10.1038/s41419-020-02737-x
Zhan, X., Wan, J., Zhang, G., Song, L., Gui, F., Zhang, Y., Li, Y., Guo, J., Dawra, R.K., Saluja, A. K., Haddock, A.N., Zhang, L., Bi, Y., Ji, B. (2019). Elevated intracellular trypsin exacerbates acute pancreatitis and chronic pancreatitis in mice. American Journal of Physiology-Gastrointestinal and Liver Physiology, 316, G816–G825. https://doi.org/10.1152/ajpgi.00004.2019
Zhang, J., Yang, X., Li, Y. (2021). Key active sites of proteases and protease inhibitors: A review. Chinese Journal of Biotechnology, 37, 561–579.

Year 2023, , 282 - 292, 08.10.2023

Ebru İlhan Lale , İsmet Burcu Turkyilmaz , Refiye Yanardağ

https://doi.org/10.3153/FH23025

Abstract

Keywords

Trypsin, plant extract, enzyme inhibition, chemical compounds.

Project Number

41104

References

Abd el-Rady, N.M., Dahpy, M.A., Ahmed, A., Elgamal, D.A., Hadiya, S., Ahmed, M.A.M., Sayed, Z.E.A.A., Abdeltawab, D., Abdelmohsen, A.S., Farrag, A.A.M., Ashmawy, A.M., Khairallah, M.K., Galal, H.M. (2021). Interplay of biochemical, genetic, and immunohistochemical factors in the etio-pathogenesis of gastric ulcer in rats: A comparative study of the effect of pomegranate loaded nanoparticles versus pomegranate peel extract. Frontiers in Physiology, 12 (649462). https://doi.org/10.3389/fphys.2021.649462
Abdellatif, F., Boudjella, H., Zitouni, A., Hassani, A. (2014). Chemical composition and antimicrobial activity of the essential oil from leaves of Algerian Melissa officinalis L. EXCLI Journal, 13, 772–781. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4464394/pdf/EXCLI-13-772.pdf
Bateman, K.S., James, M.N.G. (2011). Plant protein proteinase inhibitors: structure and mechanism of inhibition. Current Protein & Peptide Science, 12, 340–347. https://doi.org/10.2174/138920311796391124
Cid-Gallegos, M.S., Corzo-Ríos, L.J., Jiménez-Martínez, C., Sánchez-Chino, X.M. (2022). Protease inhibitors from plants as therapeutic agents - A review. Plant Foods for Human Nutrition, 77, 20–29. https://doi.org/10.1007/s11130-022-00949-4
Cheshomi, H., Bahrami, A.R., Rafatpanah, H., Matin, M.M. (2022). The effects of ellagic acid and other pomegranate (Punica granatum L.) derivatives on human gastric cancer AGS cells. Human and Experimental Toxicology, 41, 1-16. https://doi.org/10.1177/09603271211064534 Clemente, M., Corigliano, M., Pariani, S., Sánchez-López, E., Sander, V., Ramos-Duarte, V. (2019). Plant serine protease inhibitors: Biotechnology application in agriculture and molecular farming. International Journal of Molecular Sciences, 20, 1345. https://doi.org/10.3390/ijms20061345 Dağsuyu, E., Yanardağ, R. (2021). In vitro urease and trypsin inhibitory activities of some sulfur compounds. Istanbul Journal of Pharmacy, 51, 85–91. https://dergipark.org.tr/en/download/article-file/1461510
de Oliveira Braga, L.E., da Silva, G.G., de Oliveira Sousa, I.M., de Oliveira, E.C.S., Jorge, M.P., Monteiro, K.M., Sedano, T.C., Foglio, M.A., Ruiz, A.L.T.G. (2022). Gastrointestinal effects of Mentha aquatica L. essential oil. Inflammopharmacology, 30, 2127-2137. https://doi.org/10.1007/s10787-022-00989-x
Elsässer, B., Goettig, P. (2021). Mechanisms of proteolytic enzymes and their inhibition in QM/MM studies. International Journal of Molecular Sciences, 22, 3232. https://doi.org/10.3390/ijms22063232
Fan, F.Y., Huang, C.S., Tong, Y.L., Guo, H.W., Zhou, S.J., Ye, J.H., Gong, S.Y. (2021). Widely targeted metabolomics analysis of white peony teas with different storage time and association with sensory attributes. Food Chemistry, 362, 130257. https://doi.org/10.1016/j.foodchem.2021.130257
Feng, Y., Lv, M., Lu, Y., Liu, K., Liu, L., He, Z., Wu, K., Wang, X., Zhang, B., Wu, X. (2018). Characterization of binding interactions between selected phenylpropanoid glycosides and trypsin. Food Chemistry, 243, 118–124. https://doi.org/10.1016/j.foodchem.2017.09.118
Geisslitz, S., Weegels, P., Shewry, P., Zevallos, V., Masci, S., Sorrells, M., Gregorini, A., Colomba, M., Jonkers, D., Huang, X., de Giorgio, R., Caio, G.P., D’Amico, S., Larré, C., Brouns, F. (2022). Wheat amylase/trypsin inhibitors (ATIs): occurrence, function and health aspects. European Journal of Nutrition, 61, 2873-2880. https://doi.org/10.1007/s00394-022-02841-y
Ghosh, K.S., Debnath, J., Pathak, T., Dasgupta, S. (2008). Using proton nuclear magnetic resonance to study the mode of ribonuclease A inhibition by competitive and noncompetitive inhibitors. Bioorganic & Medicinal Chemistry Letters, 18, 5503–5506. https://doi.org/10.1016/j.bmcl.2008.09.014
Gräwe, A., Ranglack, J., Weber, W., Stein, V. (2020). Engineering artificial signaling functions with proteases. Current Opinion in Biotechnology, 63, 1–7. https://doi.org/10.1016/j.copbio.2019.09.017
Greene, C.M., McElvaney, N.G. (2009). Proteases and antiproteases in chronic neutrophilic lung disease - relevance to drug discovery. British Journal of Pharmacology, 158, 1048–1058. https://doi.org/10.1111/j.1476-5381.2009.00448.x
Haq, S.K., Atif, S.M., Khan, R.H. (2004). Protein proteinase inhibitor genes in combat against insects, pests, and pathogens: natural and engineered phytoprotection. Archives of Biochemistry and Biophysics, 431, 145–159. https://doi.org/10.1016/j.abb.2004.07.022
Hegyi, E., Sahin-Tóth, M. (2017). Genetic risk in chronic pancreatitis: The trypsin-dependent pathway. Digestive Diseases and Sciences, 62, 1692–1701. https://doi.org/10.1007/s10620-017-4601-3
Hovhannisyan, N., Harutyunyan, Sh., Hovhannisyan, A., Hambardzumyan, A., Chitchyan, M., Melkumyan, M., Oganezova, G., Avetisyan, N. (2009). The novel inhibitors of serine proteases. Amino Acids, 37, 531–536. https://doi.org/10.1007/s00726-009-0257-4
Kan, L., Capuano, E., Fogliano, V., Verkerk, R., Mes, J.J., Tomassen, M.M.M., Oliviero, T. (2021). Inhibition of α-glucosidases by tea polyphenols in rat intestinal extract and Caco-2 cells grown on transwell. Food Chemistry, 361, 130047. https://doi.org/10.1016/j.foodchem.2021.130047
Li, Q., Wei, Q., Yuan, E., Yang, J., Ning, Z. (2014). Interaction between four flavonoids and trypsin: effect on the characteristics of trypsin and antioxidant activity of flavonoids. International Journal of Food Science & Technology, 49, 1063–1069. https://doi.org/10.1111/ijfs.12401
Mancek-Keber, M. (2014). Inflammation-mediating proteases: Structure, function in (patho) physiology and inhibition. Protein and Peptide Letters, 21, 1209–1229. https://doi.org/10.2174/0929866521666140819123932
Mashayekhi-Sardoo, H., Razavi, B.M., Ekhtiari, M., Kheradmand, N., Imenshahidi, M. (2020). Gastroprotective effects of both aqueous and ethanolic extracts of Lemon verbena leaves against indomethacin-induced gastric ulcer in rats. Iranian Journal of Basic Medical Sciences, 23, 1639–1646. https://doi.org/10.22038/ijbms.2020.44341.10377
Navaei-Bonab, R., Kazzazi, M., Saber, M., Vatanparast, M. (2018). Differential inhibition of Helicoverpa armigera (Lep.: Noctuidae) gut digestive trypsin by extracted and purified inhibitor of datura metel (Solanales: Solanaceae). Journal of Economic Entomology, 111, 178–186. https://doi.org/10.1093/jee/tox209
Park, K.C., Dharmasivam, M., Richardson, D.R. (2020). The role of extracellular proteases in tumor progression and the development of innovative metal ion chelators that inhibit their activity. International Journal of Molecular Sciences, 21, 6805. https://doi.org/10.3390/ijms21186805
Regulski, M., Regulska, K., Stanisz, B., Murias, M., Gieremek, P., Wzgarda, A., Niznik, B. (2015). Chemistry and pharmacology of angiotensin-converting enzyme inhibitors. Current Pharmaceutical Design, 21, 1764–1775. https://doi.org/10.2174/1381612820666141112160013
Ribeiro, J.K.C., Cunha, D.D.S., Fook, J.M.S.LL., Sales, M.P. (2010). New properties of the soybean trypsin inhibitor: Inhibition of human neutrophil elastase and its effect on acute pulmonary injury. European Journal of Pharmacology, 644, 238–244. https://doi.org/10.1016/j.ejphar.2010.06.067
Saberi, A., Abbasloo, E., Sepehri, G., Yazdanpanah, M., Mirkamandari, E., Sheibani, V., Safi, Z. (2016). The Effects of methanolic extract of Melissa officinalis on experimental gastric ulcers in rats. Iranian Red Crescent Medical Journal, 18, e24271. https://doi.org/10.5812/ircmj.24271
Sacan, O., Yildiz Turhan, E. (2014). Lipoxygenase inhibitory activities of some plant extracts and chemical compounds. European Journal of Biology, 73, 47–52.
Satoh, H., Amagase, K., Takeuchi, K. (2014). Mucosal protective agents prevent exacerbation of NSAID-induced small intestinal lesions caused by antisecretory drugs in rats. Journal of Pharmacology and Experimental Therapeutics, 348, 227–235. https://doi.org/10.1124/jpet.113.208991
Srikanth, S., Chen, Z. (2016). Plant protease inhibitors in therapeutics-focus on cancer therapy. Frontiers in Pharmacology, 7, 470. https://doi.org/10.3389/fphar.2016.00470
Sultana, M.S., Millwood, R.J., Mazarei, M., Stewart, C.N. (2022). Proteinase inhibitors in legume herbivore defense: From natural to genetically engineered protectants. Plant Cell Reports, 41, 293–305. https://doi.org/10.1007/s00299-021-02800-7
Tarantino, A., Difonzo, G., Lopriore, G., Disciglio, G., Paradiso, V.M., Gambacorta, G., Caponio, F. (2020). Bioactive compounds and quality evaluation of ‘Wonderful’ pomegranate fruit and juice as affected by deficit irrigation. Journal of the Science of Food and Agriculture, 100, 5539–5545. https://doi.org/10.1002/jsfa.10606
Topaloglu, D., Turkyilmaz, I.B., Yanardag, R. (2022). Gastroprotective effect of vitamin U in D-galactosamine-induced hepatotoxicity. Journal of Biochemical and Molecular Toxicology, 36, e23124. https://doi.org/10.1002/jbt.23124
Turkyilmaz, I.B., Coskun, Z.M., Bolkent, S., Yanardag, R. (2019). The effects of antioxidant combination on indomethacin-induced gastric mucosal injury in rats. Cellular and Molecular Biology, 65, 76-83. https://doi.org/10.14715/cmb/2019.65.3.11
Uçan, F., Ağçam, E., Akyildiz, A. (2016). Bioactive compounds and quality parameters of natural cloudy lemon juices. Journal of Food Science and Technology, 53, 1465–1474. https://doi.org/10.1007/s13197-015-2155-y
Viskupicova, J., Danihelova, M., Majekova, M., Liptaj, T., Sturdik, E. (2012). Polyphenol fatty acid esters as serine protease inhibitors: a quantum-chemical QSAR analysis. Journal of Enzyme Inhibition and Medicinal Chemistry, 27, 800–809. https://doi.org/10.3109/14756366.2010.616860
Yi, C., Wu, W., Zheng, D., Peng, G., Huang, H., Shen, Z., Teng, X. (2020). Targeted inhibition of endothelial calpain delays wound healing by reducing inflammation and angiogenesis. Cell Death & Disease, 11, 533. https://doi.org/10.1038/s41419-020-02737-x
Zhan, X., Wan, J., Zhang, G., Song, L., Gui, F., Zhang, Y., Li, Y., Guo, J., Dawra, R.K., Saluja, A. K., Haddock, A.N., Zhang, L., Bi, Y., Ji, B. (2019). Elevated intracellular trypsin exacerbates acute pancreatitis and chronic pancreatitis in mice. American Journal of Physiology-Gastrointestinal and Liver Physiology, 316, G816–G825. https://doi.org/10.1152/ajpgi.00004.2019
Zhang, J., Yang, X., Li, Y. (2021). Key active sites of proteases and protease inhibitors: A review. Chinese Journal of Biotechnology, 37, 561–579.

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Details

Primary Language	English
Subjects	Structural Biology
Journal Section	Research Articles
Authors	Ebru İlhan Lale 0000-0001-7607-6886 İsmet Burcu Turkyilmaz 0000-0003-2789-5943 Refiye Yanardağ 0000-0003-4185-4363
Project Number	41104
Early Pub Date	July 27, 2023
Publication Date	October 8, 2023
Submission Date	February 3, 2023
Published in Issue	Year 2023

Cite

APA	İlhan Lale, E., Turkyilmaz, İ. B., & Yanardağ, R. (2023). In vitro trypsin inhibitory activities of some plant and fruit extracts and chemical compounds. Food and Health, 9(4), 282-292. https://doi.org/10.3153/FH23025

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