Research Article
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Increased expression of GLUT4, catalase and nitric oxide by a crystalised fraction from ethanolic extracts of oak leaf lettuce and okra seed in C2C12 cell line

Year 2024, Volume: 10 Issue: 3, 178 - 187

Jasadee Kaewsrichan Ashara Pengnoo

Abstract

The fractions obtained from low temperature-induced crystallisation of ethanolic extracts of green- and red-oak leaf and okra seed were accounted for total polyphenol content and the DPPH antioxidant activity, resulting in 5.99, 4.44, and 12.35 mg gallic acid equivalent per g sample, and 0.45, 0.35, and 0.99 mg Trolox equivalent per g sample, respectively. Insulin resistance was the result after incubating C2C12 skeletal muscle cells in high glucose DMEM for 20 h. Oxidative stress and inflammation were triggered by re-incubating these resistant cells with insulin. The expression of p-p38 MAPK and NF-B proteins and the NF-B p65 transcription factor activity were enhanced. Such inflammatory protein markers were reduced when the crystalised extracts replaced insulin. Increased catalase activity and NO production were also determined for the incubations using the crystalised extracts. It was suitable to include these vegetables in the daily diets of prediabetic individuals for better management of the patient’s health status by increasing insulin sensitivity and decreasing inflammation.

Keywords

NF-B p65 transcription factor GLUT4 Catalase activity Nitric oxide Polyphenols DPPH antioxidant activity Oak leaf lettuce Okra seed C2C12 cell line

References

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  • Chandrasekaran, A., del Pilar Sosa Idelchik, M., Andrés Melendez, J. (2017). Redox control of senescence and age-related disease. Redox Biology, 11, 91-102. https://doi.org/10.1016/j.redox.2016.11.005
  • Furman, D., Campisi, J., Verdin, E. et al. (2019). Chronic inflammation in the aetiology of disease across the life span. Nature Medicine, 25, 1822–1832. https://doi.org/10.1038/s41591-019-0675-0
  • Găman, M.A., Epîngeac, M.E., Diaconu, C.C., Găman, A.M. (2020). Evaluation of oxidative stress levels in obesity and diabetes by the free oxygen radical test and free oxygen radical defense assays and correlations with anthropometric and laboratory parameters. World Journal of Diabetes, 11(5), 193-201. https://doi.org/10.4239/wjd.v11.i5.193
  • Gayathri, G.K., Shinde, P.L., John, S., Sivakumar, K.C., Mishra, R. (2023). Understanding the combined effects of high glucose induced hyper-osmotic stress and oxygen tension in the progression of tumourigenesis: From mechanism to anti-cancer therapeutics. Cells, 12(6), 825-855. https://doi.org/10.3390/cells12060825
  • Godos, J., Caraci, F., Castellano, S., et al. (2020). Association between dietary flavonoids intake and cognitive function in an Italian cohort. Biomolecules, 10(9), 1300-1312. https://doi.org/10.3390/biom10091300
  • Iwata, M., Inoue, T., Asai, Y., Hori, K., Fujiwara, M., Matsuo, S., Tsuchida, W., Suzuki, S. (2020). The protective role of localized nitric oxide production during inflammation may be mediated by the heme oxygenase-1/carbon monoxide pathway. Biochemistry and Biophysics Reports, 23, 100790. https://doi.org/10.1016/j.bbrep.2020.100790
  • Khan, R.M.M., Chua, Z.J.Y., Tan, J.C., Yang, Y., Liao, Z., Zhao, Y. (2019). From pre-diabetes to diabetes: Diagnosis, treatments and translational research. Medicina (Kaunas), 55(9), 546-575. https://doi.org/10.3390/medicina55090546
  • Li, J., Zhao, R., Miao, P., Xu, F., Chen, J., Jiang, X., Hui, Z., Wang, L., Bai, R. (2023). Discovery of anti-inflammatory natural flavonoids: Diverse scaffolds and promising leads for drug discovery. European Journal of Medicinal Chemistry, 260, 115791. https://doi.org/10.1016/j.ejmech.2023.115791
  • Mager, W.H., de Boer, A.H., Siderius, M.H., Voss, H.P. (2000). Cellular responses to oxidative and osmotic stress. Cell Stress Chaperones, 5(2), 73-75. https://doi.org/10.1379/1466-1268(2000)005<0073:CRTOAO>2.0.CO;2
  • Merz, K.E., Thurmond, D.C. (2020). Role of Skeletal Muscle in Insulin Resistance and Glucose Uptake. Comprehensive Physiology, 10, 785–809. https://doi.org/10.1002/cphy.c190029
  • Nandi, A., Yan, L.J., Kumar Jana, C., Das, N. (2019). Role of catalase in oxidative stress- and age-associated degenerative diseases. In Oxidative Medicine and Cellular Longevity, Article ID 9613090, 19. https://doi.org/10.1155/2019/9613090
  • Owei, I., Umekwe, N., Ceesay, F., Dagogo-Jack, S. (2019). Awareness of prediabetes status and subsequent health behavior, body weight, and blood glucose levels. Journal of the American Board of Family Medicine, 32(1), 20-27. https://doi.org/10.3122/jabfm.2019.01.180242
  • Parasoglou, P., Rao, S., Slade, J.M. (2017). Declining skeletal muscle function in diabetic peripheral neuropathy. Clinical Therapeutics, 39(6), 1085-1103. https://doi.org/10.1016/j.clinthera.2017.05.001
  • Peter, E.L., Nagendrappa, P.B., Ajayi, C.O., Sesaazi, C.D. (2021). Total polyphenols and antihyperglycemic activity of aqueous fruits extract of Abelmoschus esculentus: Modeling and optimization of extraction conditions. PLoS One, 16(4), e0250405. https://doi.org/10.1371/journal.pone.0250405
  • Sami, R., Lianzhou, J., Yang, L., Ma, Y., Jing, J. (2013). Evaluation of fatty acid and amino acid compositions in okra (Abelmoschus esculentus) grown in different geographical locations. BioMed Research International, 574283. https://doi.org/10.1155/2013/574283
  • Stanaway, J.D., Afshin, A., Ashbaugh, C. et al. (2022). Health effects associated with vegetable consumption: A burden of proof study. Nature Medicine, 28, 2066-2074. https://doi.org/10.1038/s41591-022-01970-5
  • Viacava, G.E., Roura, S.I., Berrueta, L.A., Iriondo, C., Gallo, B., Alonso-Salces, R.M. (2017). Characterization of phenolic compounds in green and red oak-leaf lettuce cultivars by UHPLC-DAD-ESI-QToF/MS using MSE scan mode. Journal of Mass Spectrometry, 52(12), 873–902. https://doi.org/10.1016/j.jpba.2019.05.007
  • Yang, X., Wei, S., Liu, B. et al. (2018). A novel integrated non-targeted metabolomic analysis reveals significant metabolite variations between different lettuce (Lactuca sativa L) varieties. Horticulture Research, 5, Article number 33. https://doi.org/10.1038/s41438-018-0050-1
  • Zheng, J., Meenu, M., Xu, B. (2019). A systematic investigation on free phenolic acids and flavonoids profiles of commonly consumed edible flowers in China. Journal of Pharmaceutical and Biomedical Analysis, 172, 268-277. https://doi.org/10.1016/j.jpba.2019.05.007
  • Zim, U., Khatun, J., Khan, M.F., Hossain, M.A., Haque, M.M. (2021). Evaluation of in vitro antioxidant activity of okra mucilage and its antidiabetic and antihyperlipidemic effect in alloxan-induced diabetic mice. Food Science & Nutrition, 9, 6854–6865. https://doi.org/10.1002/fsn3.2641

Year 2024, Volume: 10 Issue: 3, 178 - 187

Jasadee Kaewsrichan Ashara Pengnoo

Abstract

References

  • Bird, S.R., Hawley, J.A. (2017). Update on the effects of physical activity on insulin sensitivity in humans. BMJ Open Sport & Exercise Medicine, 2, e000143. https://doi.org/10.1136/bmjsem-2016-000143
  • Centers for Disease Control and Prevention. National Diabetes Statistics Report. Atlanta, GA: Centers for Disease Control and Prevention, US Department of Health and Human Services. (2017). https://nationaldppcsc.cdc.gov/article/CDC-National-Diabetes-Statistics-Report (Accessed 01.12.2023).
  • Chandrasekaran, A., del Pilar Sosa Idelchik, M., Andrés Melendez, J. (2017). Redox control of senescence and age-related disease. Redox Biology, 11, 91-102. https://doi.org/10.1016/j.redox.2016.11.005
  • Furman, D., Campisi, J., Verdin, E. et al. (2019). Chronic inflammation in the aetiology of disease across the life span. Nature Medicine, 25, 1822–1832. https://doi.org/10.1038/s41591-019-0675-0
  • Găman, M.A., Epîngeac, M.E., Diaconu, C.C., Găman, A.M. (2020). Evaluation of oxidative stress levels in obesity and diabetes by the free oxygen radical test and free oxygen radical defense assays and correlations with anthropometric and laboratory parameters. World Journal of Diabetes, 11(5), 193-201. https://doi.org/10.4239/wjd.v11.i5.193
  • Gayathri, G.K., Shinde, P.L., John, S., Sivakumar, K.C., Mishra, R. (2023). Understanding the combined effects of high glucose induced hyper-osmotic stress and oxygen tension in the progression of tumourigenesis: From mechanism to anti-cancer therapeutics. Cells, 12(6), 825-855. https://doi.org/10.3390/cells12060825
  • Godos, J., Caraci, F., Castellano, S., et al. (2020). Association between dietary flavonoids intake and cognitive function in an Italian cohort. Biomolecules, 10(9), 1300-1312. https://doi.org/10.3390/biom10091300
  • Iwata, M., Inoue, T., Asai, Y., Hori, K., Fujiwara, M., Matsuo, S., Tsuchida, W., Suzuki, S. (2020). The protective role of localized nitric oxide production during inflammation may be mediated by the heme oxygenase-1/carbon monoxide pathway. Biochemistry and Biophysics Reports, 23, 100790. https://doi.org/10.1016/j.bbrep.2020.100790
  • Khan, R.M.M., Chua, Z.J.Y., Tan, J.C., Yang, Y., Liao, Z., Zhao, Y. (2019). From pre-diabetes to diabetes: Diagnosis, treatments and translational research. Medicina (Kaunas), 55(9), 546-575. https://doi.org/10.3390/medicina55090546
  • Li, J., Zhao, R., Miao, P., Xu, F., Chen, J., Jiang, X., Hui, Z., Wang, L., Bai, R. (2023). Discovery of anti-inflammatory natural flavonoids: Diverse scaffolds and promising leads for drug discovery. European Journal of Medicinal Chemistry, 260, 115791. https://doi.org/10.1016/j.ejmech.2023.115791
  • Mager, W.H., de Boer, A.H., Siderius, M.H., Voss, H.P. (2000). Cellular responses to oxidative and osmotic stress. Cell Stress Chaperones, 5(2), 73-75. https://doi.org/10.1379/1466-1268(2000)005<0073:CRTOAO>2.0.CO;2
  • Merz, K.E., Thurmond, D.C. (2020). Role of Skeletal Muscle in Insulin Resistance and Glucose Uptake. Comprehensive Physiology, 10, 785–809. https://doi.org/10.1002/cphy.c190029
  • Nandi, A., Yan, L.J., Kumar Jana, C., Das, N. (2019). Role of catalase in oxidative stress- and age-associated degenerative diseases. In Oxidative Medicine and Cellular Longevity, Article ID 9613090, 19. https://doi.org/10.1155/2019/9613090
  • Owei, I., Umekwe, N., Ceesay, F., Dagogo-Jack, S. (2019). Awareness of prediabetes status and subsequent health behavior, body weight, and blood glucose levels. Journal of the American Board of Family Medicine, 32(1), 20-27. https://doi.org/10.3122/jabfm.2019.01.180242
  • Parasoglou, P., Rao, S., Slade, J.M. (2017). Declining skeletal muscle function in diabetic peripheral neuropathy. Clinical Therapeutics, 39(6), 1085-1103. https://doi.org/10.1016/j.clinthera.2017.05.001
  • Peter, E.L., Nagendrappa, P.B., Ajayi, C.O., Sesaazi, C.D. (2021). Total polyphenols and antihyperglycemic activity of aqueous fruits extract of Abelmoschus esculentus: Modeling and optimization of extraction conditions. PLoS One, 16(4), e0250405. https://doi.org/10.1371/journal.pone.0250405
  • Sami, R., Lianzhou, J., Yang, L., Ma, Y., Jing, J. (2013). Evaluation of fatty acid and amino acid compositions in okra (Abelmoschus esculentus) grown in different geographical locations. BioMed Research International, 574283. https://doi.org/10.1155/2013/574283
  • Stanaway, J.D., Afshin, A., Ashbaugh, C. et al. (2022). Health effects associated with vegetable consumption: A burden of proof study. Nature Medicine, 28, 2066-2074. https://doi.org/10.1038/s41591-022-01970-5
  • Viacava, G.E., Roura, S.I., Berrueta, L.A., Iriondo, C., Gallo, B., Alonso-Salces, R.M. (2017). Characterization of phenolic compounds in green and red oak-leaf lettuce cultivars by UHPLC-DAD-ESI-QToF/MS using MSE scan mode. Journal of Mass Spectrometry, 52(12), 873–902. https://doi.org/10.1016/j.jpba.2019.05.007
  • Yang, X., Wei, S., Liu, B. et al. (2018). A novel integrated non-targeted metabolomic analysis reveals significant metabolite variations between different lettuce (Lactuca sativa L) varieties. Horticulture Research, 5, Article number 33. https://doi.org/10.1038/s41438-018-0050-1
  • Zheng, J., Meenu, M., Xu, B. (2019). A systematic investigation on free phenolic acids and flavonoids profiles of commonly consumed edible flowers in China. Journal of Pharmaceutical and Biomedical Analysis, 172, 268-277. https://doi.org/10.1016/j.jpba.2019.05.007
  • Zim, U., Khatun, J., Khan, M.F., Hossain, M.A., Haque, M.M. (2021). Evaluation of in vitro antioxidant activity of okra mucilage and its antidiabetic and antihyperlipidemic effect in alloxan-induced diabetic mice. Food Science & Nutrition, 9, 6854–6865. https://doi.org/10.1002/fsn3.2641
There are 22 citations in total.

Details

Primary Language English
Subjects Biochemistry and Cell Biology (Other), Dietetics
Journal Section Research Articles
Authors

Jasadee Kaewsrichan 0000-0003-4316-3814

Ashara Pengnoo 0000-0003-1856-1327

Early Pub Date May 30, 2024
Publication Date
Submission Date December 12, 2023
Acceptance Date April 23, 2024
Published in Issue Year 2024Volume: 10 Issue: 3

Cite

APA Kaewsrichan, J., & Pengnoo, A. (2024). Increased expression of GLUT4, catalase and nitric oxide by a crystalised fraction from ethanolic extracts of oak leaf lettuce and okra seed in C2C12 cell line. Food and Health, 10(3), 178-187.

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