Year 2020, Volume 6 , Issue 4, Pages 287 - 298 2020-09-17

A co-culture study to determine the supportive role of probiotics on immune system against cancer cells

Ayşe NALBANTSOY [1] , Cenk Serhan ÖZVEREL [2] , Duygu KIŞLA [3]


In this study, two different in vitro co-culture systems were established in order to determine the supportive role of probiotic Lactobacillus acidophilus LA-5 on macrophages when used in combination with doxorubicin in both human- and mouse-origin cancerous cell lines. First, a co-culture study was undertaken using RAW 264.7 mouse macrophage cells that activated by LA-5 against 4T1 mouse triple negative breast adenocarcinoma cells. After the initial study, a second co-culture was performed using THP-1 human monocyte-like cells activated by LA-5 against CaCo-2 human colorectal adenocarcinoma cells. The supplementary potential of LA-5 on the macrophages was evaluated by investigating the viabilities of 4T1 and CaCo-2 cells via an MTT test. The data obtained in the study indicated that the viability of cancerous cells 4T1 and CaCo-2 decreased to ≈40% and ≈30% in the groups that used LPS-activated macrophages stimulated with LA-5 and the combination of LA-5 and doxorubicin (1/2 IC50), respectively. In conclusion, the use of LA-5 could be encouraged as a dietary supplementary for cancer patients due to its superior effects on immune stimulation.
Probiotics, Lactobacillus acidophilus LA-5, Immune system, Cancer cells
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Primary Language en
Subjects Science, Food Science and Technology
Journal Section Research Articles
Authors

Orcid: 0000-0002-3852-1974
Author: Ayşe NALBANTSOY
Institution: Ege University, Department of Bioengineering, Faculty of Engineering, İzmir
Country: Turkey


Orcid: 0000-0001-9932-4774
Author: Cenk Serhan ÖZVEREL
Institution: Near East University, Faculty of Dentistry, Department of Basic Medical Sciences, Nicosia
Country: KKTC


Orcid: 0000-0002-6993-3883
Author: Duygu KIŞLA
Institution: Ege University, Faculty of Engineering, Department of Food Engineering, İzmir
Country: Turkey


Dates

Application Date : May 4, 2020
Acceptance Date : July 11, 2020
Publication Date : September 17, 2020

Bibtex @research article { jfhs732082, journal = {Food and Health}, issn = {}, eissn = {2602-2834}, address = {Vidin Caddesi No:28 D:4 Kocamustafapaşa 34107 Fatih İstanbul}, publisher = {Özkan ÖZDEN}, year = {2020}, volume = {6}, pages = {287 - 298}, doi = {10.3153/FH20028}, title = {A co-culture study to determine the supportive role of probiotics on immune system against cancer cells}, key = {cite}, author = {Nalbantsoy, Ayşe and Özverel, Cenk Serhan and Kışla, Duygu} }
APA Nalbantsoy, A , Özverel, C , Kışla, D . (2020). A co-culture study to determine the supportive role of probiotics on immune system against cancer cells . Food and Health , 6 (4) , 287-298 . DOI: 10.3153/FH20028
MLA Nalbantsoy, A , Özverel, C , Kışla, D . "A co-culture study to determine the supportive role of probiotics on immune system against cancer cells" . Food and Health 6 (2020 ): 287-298 <http://jfhs.scientificwebjournals.com/en/pub/issue/56072/732082>
Chicago Nalbantsoy, A , Özverel, C , Kışla, D . "A co-culture study to determine the supportive role of probiotics on immune system against cancer cells". Food and Health 6 (2020 ): 287-298
RIS TY - JOUR T1 - A co-culture study to determine the supportive role of probiotics on immune system against cancer cells AU - Ayşe Nalbantsoy , Cenk Serhan Özverel , Duygu Kışla Y1 - 2020 PY - 2020 N1 - doi: 10.3153/FH20028 DO - 10.3153/FH20028 T2 - Food and Health JF - Journal JO - JOR SP - 287 EP - 298 VL - 6 IS - 4 SN - -2602-2834 M3 - doi: 10.3153/FH20028 UR - https://doi.org/10.3153/FH20028 Y2 - 2020 ER -
EndNote %0 Food and Health A co-culture study to determine the supportive role of probiotics on immune system against cancer cells %A Ayşe Nalbantsoy , Cenk Serhan Özverel , Duygu Kışla %T A co-culture study to determine the supportive role of probiotics on immune system against cancer cells %D 2020 %J Food and Health %P -2602-2834 %V 6 %N 4 %R doi: 10.3153/FH20028 %U 10.3153/FH20028
ISNAD Nalbantsoy, Ayşe , Özverel, Cenk Serhan , Kışla, Duygu . "A co-culture study to determine the supportive role of probiotics on immune system against cancer cells". Food and Health 6 / 4 (September 2020): 287-298 . https://doi.org/10.3153/FH20028
AMA Nalbantsoy A , Özverel C , Kışla D . A co-culture study to determine the supportive role of probiotics on immune system against cancer cells. Food Health. 2020; 6(4): 287-298.
Vancouver Nalbantsoy A , Özverel C , Kışla D . A co-culture study to determine the supportive role of probiotics on immune system against cancer cells. Food and Health. 2020; 6(4): 287-298.