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Evaluation of Therapeutic Effect of Chrysin Against 5-Fluorouracil-Induced Ovarian Damage in Rats

Year 2023, Volume: 2 Issue: 1, 1 - 7, 30.03.2023

Elif Ayazoglu Demir Ahmet Mentese Selim Demir Hatice Küçük Nihal Türkmen Yüksel Aliyazıcıoğlu

Abstract

5-fluorouracil (5-FU) is an effective and widely used chemotherapeutic agent to treat various malignancies, but its therapeutic use is limited due to dose-related tissue toxicity. Many studies have confirmed that oxidative stress and inflammation play a major role in the pathogenesis of 5-FU-induced damage in the various tissues. Chrysin (CHS), a natural flavone, exhibits various beneficial activities, including antioxidant, anti-inflammatory and anticancer. The aim of this study was to determine the therapeutic effect of CHS against 5-FU-induced oxidative stress and inflammation in the ovary tissue of rats for the first time. Thirty female rats were divided into 5 groups: control, 5-FU (100 mg/kg), 5-FU+CHS (1 mg/kg), 5-FU+CHS (2 mg/kg) and CHS (2 mg/kg). 5-FU treatment was administered intraperitoneally (i.p.) on the first day and CHS (i.p.) were applied for the following 3 days. The ovarian tissue levels of malondialdehyde (MDA), total oxidant status (TOS), total antioxidant status (TAS), 8-hydroxy-2'-deoxyguanosine (8-OHdG), catalase (CAT) and interleukin-6 (IL-6) were determined using spectrophotometric methods. MDA, TOS, 8-OHdG and IL-6 levels were significantly higher (p<0.05) and TAS and CAT levels level were significantly lower (p<0.05) in the 5-FU group than in the control group. CHS treatments significantly restored the levels of oxidative stress and inflammation parameters in a dose-dependent manner (p<0.05). Our results suggest that CHS can have a therapeutic effect against 5-FU-induced ovarian damage and therefore the use of CHS after chemotherapy may be beneficial in abolishing 5-FU-induced reproductive toxicity.

Keywords

5-fluorouracil Chrysin Inflammation Ovarian damage Oxidative stress

References

  • El-Sayyad HI, El-Sherbiny MR, Abou-El-Naga AM, Gadallah AA, Areida ESK. Effects of adriamycin, cisplatin, and 5-fluorouracil on the testes of albino rats. British J Med Health Res. 2013; 1(5): 45-62.
  • Temel Y, Kucukler S, Yıldırım S, Caglayan C, Kandemir FM. Protective effect of chrysin on cyclophosphamide-induced hepatotoxicity and nephrotoxicity via the inhibition of oxidative stress, inflammation, and apoptosis. Naunyn Schmiedebergs Arch Pharmacol. 2020; 393(3): 325-337. DOI: 10.1007/s00210-019-01741-z.
  • El-Sayyad HI, Ismail MF, Shalaby FM, et al. Histopathological effects of cisplatin, doxorubicin and 5-flurouracil (5-FU) on the liver of male albino rats. Int J Biol Sci. 2009; 5(5): 466-473. DOI: 10.7150/ijbs.5.466.
  • Gedikli S, Erbas E. Protective effects of naringin on lung toxicity induced by 5-fluorouracil in rats. Kocatepe Vet J. 2021; 14(1): 16-25. DOI: 10.30607/kvj.782356.
  • Al-Asmari AK, Khan AQ, Al-Masri N. Mitigation of 5-fluorouracil-induced liver damage in rats by vitamin C via targeting redox-sensitive transcription factors. Hum Exp Toxicol. 2016; 35(11): 1203-1213. DOI: 10.1177/0960327115626583.
  • Lambouras M, Liew SH, Horvay K, Abud HE, Stringer JM, Hutt KJ. Examination of the ovotoxicity of 5-fluorouracil in mice. J Assist Reprod Genet. 2018; 35: 1053-1060. DOI: 10.1007/s10815-018-1169-6.
  • Meirow D, Biederman H, Anderson RA, Wallace WHB. Toxicity of chemotherapy and radiation on female reproduction. Clin Obstet Gynecol. 2010; 53(4): 727-739. DOI: 10.1097/GRF.0b013e3181f96b54.
  • Stringer JM, Swindells EOK, Zerafa N, Liew SH, Hutt KJ. Multidose 5-fluorouracil is highly toxic to growing ovarian follicles in mice. Toxicol Sci. 2018; 166(1): 97-107. DOI: 10.1093/toxsci/kfy189.
  • Jurczyk M, Król M, Midro A, Kurnik-Łucka M, Poniatowski A, Gil K. Cardiotoxicity of fluoropyrimidines: Epidemiology, mechanisms, diagnosis, and management. J Clin Med. 2021; 10(19): 4426. DOI: 10.3390/jcm10194426.
  • Mentese A, Demir S, Alemdar NT, Aliyazicioglu Y, Deger O. The effect of chlorogenic acid on 5-fluorouracil-induced oxidative damage in rat ovarian tissue. Farabi Med J. 2022; 1(1): 1-7.
  • Rashid S, Ali N, Nafees S, et al. Alleviation of doxorubicin-induced nephrotoxicity and hepatotoxicity by chrysin in Wistar rats. Toxicol Mech Methods. 2013; 23(5): 337-345. DOI: 10.3109/15376516.2012.759306.
  • Rehman MU, Ali N, Rashid S, et al. Alleviation of hepatic injury by chrysin in cisplatin administered rats: probable role of oxidative and inflammatory markers. Pharmacol Rep. 2014; 66(6): 1050-1059. DOI: 10.1016/j.pharep.2014.06.004.
  • Samarghandian S, Farkhondeh T, Azimi-Nezhad M. Protective effects of chrysin against drugs and toxic agents. Dose Response. 2017; 15(2): 1-10. DOI: 10.1177/1559325817711782.
  • Zeinali M, Rezaee SA, Hosseinzadeh H. An overview on immunoregulatory and anti-inflammatory properties of chrysin and flavonoids substances. Biomed Pharmacother. 2017; 92: 998-1009. DOI: 10.1016/j.biopha.2017.06.003.
  • Pingili RB, Pawar AK, Challa SR, Kodali T, Koppula S, Toleti V. A comprehensive review on hepatoprotective and nephroprotective activities of chrysin against various drugs and toxic agents. Chem Biol Interact. 2019; 308: 51-60. DOI: 10.1016/j.cbi.2019.05.010.
  • Sultana S, Verma K, Khan R. Nephroprotective efficacy of chrysin against cisplatin-induced toxicity via attenuation of oxidative stress. J Pharm Pharmacol. 2012; 64(6): 872-881. DOI: 10.1111/j.2042-7158.2012.01470.x.
  • Rashid S, Ali N, Nafees S, Hasan SK, Sultana S. Mitigation of 5-fluorouracil induced renal toxicity by chrysin via targeting oxidative stress and apoptosis in wistar rats. Food Chem Toxicol. 2014; 66: 185-193. DOI: 10.1016/j.fct.2014.01.026.
  • Ali N, Rashid S, Nafees S, Hasan SK, Sultana S. Beneficial effects of chrysin against methotrexate-induced hepatotoxicity via attenuation of oxidative stress and apoptosis. Mol Cell Biochem. 2014; 385(1-2): 215-223. DOI: 10.1007/s11010-013-1830-4.
  • Mantawy EM, Esmat A, El-Bakly WM, Salah-Eldin RA, El-Demerdash E. Mechanistic clues to the protective effect of chrysin against doxorubicin-induced cardiomyopathy: Plausible roles of p53, MAPK and AKT pathways. Sci Rep. 2017; 7(1): 4795. DOI: 10.1038/s41598-017-05005-9.
  • Mentese A, Alemdar NT, Livaoglu A, Ayazoglu Demir E, Aliyazicioglu Y, Demir S. Suppression of cisplatin-induced ovarian injury in rats by chrysin: an experimental study. J Obstet Gynaecol. 2022; doi:10.1080/01443615.2022.2130201, In Press.
  • Zhang S, Liu Y, Xiang D, et al. Assessment of dose-response relationship of 5-fluorouracil to murine intestinal injury. Biomed Pharmacother. 2018; 106: 910-916. DOI: 10.1016/j.biopha.2018.07.029.
  • Narayana K, D'Souza UJ, Sanyal AK, Rao KP. 5-fluorouracil (5-FU) induces the formation of giant cells and sloughing of seminiferous epithelium in the rat testis. Indian J Physiol Pharmacol. 2000; 44(3): 317-322.
  • Rodríguez-Landa JF, Hernández-López F, Cueto-Escobedo J, et al. Chrysin (5,7-dihydroxyflavone) exerts anxiolytic-like effects through GABAA receptors in a surgical menopause model in rats. Biomed Pharmacother. 2019; 109: 2387-2395. DOI: 10.1016/j.biopha.2018.11.111.
  • Ayazoglu Demir E, Mentese A, Livaoglu A, Alemdar NT, Demir S. Ameliorative effect of gallic acid on cisplatin-induced ovarian toxicity in rats. Drug Chem Toxicol. 2023; 46(1): 97-103. DOI: 10.1080/01480545.2021.2011312.
  • Mihara M, Uchiyama M. Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal Biochem. 1978; 86(1): 271-278. DOI: 10.1016/0003-2697(78)90342-1.
  • Demir S, Kazaz IO, Aliyazicioglu Y, et al. Effect of ethyl pyruvate on oxidative state and endoplasmic reticulum stress in a rat model of testicular torsion. Biotech Histochem. 2020; 95(4): 317-322. DOI: 10.1080/10520295.2019.1695947.
  • Demir S, Kazaz IO, Kerimoglu G, et al. Astaxanthin protects testicular tissue against torsion/detorsion-induced injury via suppressing endoplasmic reticulum stress in rats. J Invest Surg. 2022; 35(5): 1044-1049. DOI: 10.1080/08941939.2021.1995540.
  • Ayazoglu Demir E, Mentese A, Kucuk H, Alemdar NT, Demir S. p-Coumaric acid alleviates cisplatin-induced ovarian toxicity in rats. J Obstet Gynaecol Res. 2022; 48(2): 411-419. DOI: 10.1111/jog.15119.
  • Gelen V, Şengül E, Yıldırım S, Senturk E, Tekin S, Kükürt A. The protective effects of hesperidin and curcumin on 5-fluorouracil-induced nephrotoxicity in mice. Environ Sci Pollut Res Int. 2021; 28(34): 47046-47055. DOI: 10.1007/s11356-021-13969-5.
  • Barary M, Hosseinzadeh R, Kazemi S, et al. The effect of propolis on 5-fluorouracil-induced cardiac toxicity in rats. Sci Rep. 2022; 12(1): 8661. DOI: 10.1038/s41598-022-12735-y.
  • Khan R, Khan AQ, Qamar W, et al. Chrysin protects against cisplatin-induced colon toxicity via amelioration of oxidative stress and apoptosis: probable role of p38MAPK and p53. Toxicol Appl Pharmacol. 2012; 258(3): 315-329. DOI: 10.1016/j.taap.2011.11.013.
  • Mantawy EM, El-Bakly WM, Esmat A, Badr AM, El-Demerdash E. Chrysin alleviates acute doxorubicin cardiotoxicity in rats via suppression of oxidative stress, inflammation and apoptosis. Eur J Pharmacol. 2014; 728: 107-118. DOI: 10.1016/j.ejphar.2014.01.065.
  • Belhan S, Çomaklı S, Küçükler S, Gülyüz F, Yıldırım S, Yener Z. Effect of chrysin on methotrexate-induced testicular damage in rats. Andrologia. 2019; 51(1): e13145. DOI: 10.1111/and.13145.
  • Baykalir BG, Arslan AS, Mutlu SI, et al. The protective effect of chrysin against carbon tetrachloride-induced kidney and liver tissue damage in rats. Int J Vitam Nutr Res. 2021; 91(5-6): 427-438. DOI: 10.1024/0300-9831/a000653.
  • Rapa SF, Magliocca G, Pepe G, et al. Protective effect of pomegranate on oxidative stress and inflammatory response induced by 5-fluorouracil in human keratinocytes. Antioxidants (Basel). 2021; 10(2): 203. DOI: 10.3390/antiox10020203.
  • Kucukler S, Benzer F, Yildirim S, et al. Protective effects of chrysin against oxidative stress and inflammation induced by lead acetate in rat kidneys: A biochemical and histopathological approach. Biol Trace Elem Res. 2021; 199(4): 1501-1514. DOI: 10.1007/s12011-020-02268-8.
  • Belhan S, Ozkaraca M, Ozdek U, Komuroglu AU. Protective role of chrysin on doxorubicin-induced oxidative stress and DNA damage in rat testes. Andrologia. 2020; 52(9): e13747. DOI: 10.1111/and.13747.

Sıçanlarda 5-Florourasil Kaynaklı Yumurtalık Hasarına Karşı Krisinin Terapötik Etkisinin Değerlendirilmesi

Year 2023, Volume: 2 Issue: 1, 1 - 7, 30.03.2023

Elif Ayazoglu Demir Ahmet Mentese Selim Demir Hatice Küçük Nihal Türkmen Yüksel Aliyazıcıoğlu

Abstract

5-florourasil (5-FU), çeşitli maligniteleri tedavi etmek için etkili ve yaygın olarak kullanılan bir kemoterapötik ajandır, ancak doza bağlı doku toksisitesi nedeniyle terapötik kullanımı sınırlıdır. Birçok çalışma, çeşitli dokularda 5-FU'nun neden olduğu hasarın patogenezinde oksidatif stres ve inflamasyonun önemli bir rol oynadığını doğrulamıştır. Doğal bir flavon olan krisin (CHS), antioksidan, anti-inflamatuvar ve antikanser de dahil olmak üzere çeşitli faydalı aktiviteler sergileyebilmektedir. Bu çalışmanın amacı, sıçanların yumurtalık dokusunda 5-FU ile indüklenen oksidatif stres ve inflamasyona karşı CHS'nin terapötik etkisini ilk kez belirlemekti. Otuz adet dişi sıçan kontrol, 5-FU (100 mg/kg), 5-FU+CHS (1 mg/kg), 5-FU+CHS (2 mg/kg) ve CHS (2 mg/kg) olmak üzere 5 gruba ayrıldı. İlk gün intraperitoneal (i.p.) 5-FU tedavisi, takip eden 3 gün CHS (i.p.) uygulandı. Yumurtalık dokularında malondialdehit (MDA), toplam oksidan durum (TOS), toplam antioksidan durum (TAS), 8-hidroksi-2'-deoksiguanozin (8-OHdG), katalaz (CAT) ve interlökin-6 (IL-6) seviyeleri spektrofotometrik yöntemlerle belirlendi. 5-FU grubunda kontrol grubuna göre MDA, TOS, 8-OHdG ve IL-6 düzeyleri anlamlı olarak yüksek iken (p<0,05), TAS ve CAT düzeyleri ise istatistiksel olarak anlamlı düzeyde düşüktü (p<0,05). CHS tedavileri, oksidatif stres ve inflamasyon parametrelerinin seviyelerini doza bağlı bir şekilde istatistiksel olarak anlamlı derecede düzeltti (p<0,05). Sonuçlarımız, CHS'nin 5-FU ile indüklenen yumurtalık hasarına karşı terapötik bir etkiye sahip olabileceğini ve bu nedenle kemoterapiden sonra CHS kullanımının 5-FU ile indüklenen üreme toksisitesini ortadan kaldırmada faydalı olabileceğini düşündürmektedir.

Keywords

5-florourasil İnflamasyon Krisin Oksidatif stress Rat Yumurtalık dokusu hasarı 5-fluorouracil Chrysin Inflammation Ovarian damage Oxidative stress

References

  • El-Sayyad HI, El-Sherbiny MR, Abou-El-Naga AM, Gadallah AA, Areida ESK. Effects of adriamycin, cisplatin, and 5-fluorouracil on the testes of albino rats. British J Med Health Res. 2013; 1(5): 45-62.
  • Temel Y, Kucukler S, Yıldırım S, Caglayan C, Kandemir FM. Protective effect of chrysin on cyclophosphamide-induced hepatotoxicity and nephrotoxicity via the inhibition of oxidative stress, inflammation, and apoptosis. Naunyn Schmiedebergs Arch Pharmacol. 2020; 393(3): 325-337. DOI: 10.1007/s00210-019-01741-z.
  • El-Sayyad HI, Ismail MF, Shalaby FM, et al. Histopathological effects of cisplatin, doxorubicin and 5-flurouracil (5-FU) on the liver of male albino rats. Int J Biol Sci. 2009; 5(5): 466-473. DOI: 10.7150/ijbs.5.466.
  • Gedikli S, Erbas E. Protective effects of naringin on lung toxicity induced by 5-fluorouracil in rats. Kocatepe Vet J. 2021; 14(1): 16-25. DOI: 10.30607/kvj.782356.
  • Al-Asmari AK, Khan AQ, Al-Masri N. Mitigation of 5-fluorouracil-induced liver damage in rats by vitamin C via targeting redox-sensitive transcription factors. Hum Exp Toxicol. 2016; 35(11): 1203-1213. DOI: 10.1177/0960327115626583.
  • Lambouras M, Liew SH, Horvay K, Abud HE, Stringer JM, Hutt KJ. Examination of the ovotoxicity of 5-fluorouracil in mice. J Assist Reprod Genet. 2018; 35: 1053-1060. DOI: 10.1007/s10815-018-1169-6.
  • Meirow D, Biederman H, Anderson RA, Wallace WHB. Toxicity of chemotherapy and radiation on female reproduction. Clin Obstet Gynecol. 2010; 53(4): 727-739. DOI: 10.1097/GRF.0b013e3181f96b54.
  • Stringer JM, Swindells EOK, Zerafa N, Liew SH, Hutt KJ. Multidose 5-fluorouracil is highly toxic to growing ovarian follicles in mice. Toxicol Sci. 2018; 166(1): 97-107. DOI: 10.1093/toxsci/kfy189.
  • Jurczyk M, Król M, Midro A, Kurnik-Łucka M, Poniatowski A, Gil K. Cardiotoxicity of fluoropyrimidines: Epidemiology, mechanisms, diagnosis, and management. J Clin Med. 2021; 10(19): 4426. DOI: 10.3390/jcm10194426.
  • Mentese A, Demir S, Alemdar NT, Aliyazicioglu Y, Deger O. The effect of chlorogenic acid on 5-fluorouracil-induced oxidative damage in rat ovarian tissue. Farabi Med J. 2022; 1(1): 1-7.
  • Rashid S, Ali N, Nafees S, et al. Alleviation of doxorubicin-induced nephrotoxicity and hepatotoxicity by chrysin in Wistar rats. Toxicol Mech Methods. 2013; 23(5): 337-345. DOI: 10.3109/15376516.2012.759306.
  • Rehman MU, Ali N, Rashid S, et al. Alleviation of hepatic injury by chrysin in cisplatin administered rats: probable role of oxidative and inflammatory markers. Pharmacol Rep. 2014; 66(6): 1050-1059. DOI: 10.1016/j.pharep.2014.06.004.
  • Samarghandian S, Farkhondeh T, Azimi-Nezhad M. Protective effects of chrysin against drugs and toxic agents. Dose Response. 2017; 15(2): 1-10. DOI: 10.1177/1559325817711782.
  • Zeinali M, Rezaee SA, Hosseinzadeh H. An overview on immunoregulatory and anti-inflammatory properties of chrysin and flavonoids substances. Biomed Pharmacother. 2017; 92: 998-1009. DOI: 10.1016/j.biopha.2017.06.003.
  • Pingili RB, Pawar AK, Challa SR, Kodali T, Koppula S, Toleti V. A comprehensive review on hepatoprotective and nephroprotective activities of chrysin against various drugs and toxic agents. Chem Biol Interact. 2019; 308: 51-60. DOI: 10.1016/j.cbi.2019.05.010.
  • Sultana S, Verma K, Khan R. Nephroprotective efficacy of chrysin against cisplatin-induced toxicity via attenuation of oxidative stress. J Pharm Pharmacol. 2012; 64(6): 872-881. DOI: 10.1111/j.2042-7158.2012.01470.x.
  • Rashid S, Ali N, Nafees S, Hasan SK, Sultana S. Mitigation of 5-fluorouracil induced renal toxicity by chrysin via targeting oxidative stress and apoptosis in wistar rats. Food Chem Toxicol. 2014; 66: 185-193. DOI: 10.1016/j.fct.2014.01.026.
  • Ali N, Rashid S, Nafees S, Hasan SK, Sultana S. Beneficial effects of chrysin against methotrexate-induced hepatotoxicity via attenuation of oxidative stress and apoptosis. Mol Cell Biochem. 2014; 385(1-2): 215-223. DOI: 10.1007/s11010-013-1830-4.
  • Mantawy EM, Esmat A, El-Bakly WM, Salah-Eldin RA, El-Demerdash E. Mechanistic clues to the protective effect of chrysin against doxorubicin-induced cardiomyopathy: Plausible roles of p53, MAPK and AKT pathways. Sci Rep. 2017; 7(1): 4795. DOI: 10.1038/s41598-017-05005-9.
  • Mentese A, Alemdar NT, Livaoglu A, Ayazoglu Demir E, Aliyazicioglu Y, Demir S. Suppression of cisplatin-induced ovarian injury in rats by chrysin: an experimental study. J Obstet Gynaecol. 2022; doi:10.1080/01443615.2022.2130201, In Press.
  • Zhang S, Liu Y, Xiang D, et al. Assessment of dose-response relationship of 5-fluorouracil to murine intestinal injury. Biomed Pharmacother. 2018; 106: 910-916. DOI: 10.1016/j.biopha.2018.07.029.
  • Narayana K, D'Souza UJ, Sanyal AK, Rao KP. 5-fluorouracil (5-FU) induces the formation of giant cells and sloughing of seminiferous epithelium in the rat testis. Indian J Physiol Pharmacol. 2000; 44(3): 317-322.
  • Rodríguez-Landa JF, Hernández-López F, Cueto-Escobedo J, et al. Chrysin (5,7-dihydroxyflavone) exerts anxiolytic-like effects through GABAA receptors in a surgical menopause model in rats. Biomed Pharmacother. 2019; 109: 2387-2395. DOI: 10.1016/j.biopha.2018.11.111.
  • Ayazoglu Demir E, Mentese A, Livaoglu A, Alemdar NT, Demir S. Ameliorative effect of gallic acid on cisplatin-induced ovarian toxicity in rats. Drug Chem Toxicol. 2023; 46(1): 97-103. DOI: 10.1080/01480545.2021.2011312.
  • Mihara M, Uchiyama M. Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal Biochem. 1978; 86(1): 271-278. DOI: 10.1016/0003-2697(78)90342-1.
  • Demir S, Kazaz IO, Aliyazicioglu Y, et al. Effect of ethyl pyruvate on oxidative state and endoplasmic reticulum stress in a rat model of testicular torsion. Biotech Histochem. 2020; 95(4): 317-322. DOI: 10.1080/10520295.2019.1695947.
  • Demir S, Kazaz IO, Kerimoglu G, et al. Astaxanthin protects testicular tissue against torsion/detorsion-induced injury via suppressing endoplasmic reticulum stress in rats. J Invest Surg. 2022; 35(5): 1044-1049. DOI: 10.1080/08941939.2021.1995540.
  • Ayazoglu Demir E, Mentese A, Kucuk H, Alemdar NT, Demir S. p-Coumaric acid alleviates cisplatin-induced ovarian toxicity in rats. J Obstet Gynaecol Res. 2022; 48(2): 411-419. DOI: 10.1111/jog.15119.
  • Gelen V, Şengül E, Yıldırım S, Senturk E, Tekin S, Kükürt A. The protective effects of hesperidin and curcumin on 5-fluorouracil-induced nephrotoxicity in mice. Environ Sci Pollut Res Int. 2021; 28(34): 47046-47055. DOI: 10.1007/s11356-021-13969-5.
  • Barary M, Hosseinzadeh R, Kazemi S, et al. The effect of propolis on 5-fluorouracil-induced cardiac toxicity in rats. Sci Rep. 2022; 12(1): 8661. DOI: 10.1038/s41598-022-12735-y.
  • Khan R, Khan AQ, Qamar W, et al. Chrysin protects against cisplatin-induced colon toxicity via amelioration of oxidative stress and apoptosis: probable role of p38MAPK and p53. Toxicol Appl Pharmacol. 2012; 258(3): 315-329. DOI: 10.1016/j.taap.2011.11.013.
  • Mantawy EM, El-Bakly WM, Esmat A, Badr AM, El-Demerdash E. Chrysin alleviates acute doxorubicin cardiotoxicity in rats via suppression of oxidative stress, inflammation and apoptosis. Eur J Pharmacol. 2014; 728: 107-118. DOI: 10.1016/j.ejphar.2014.01.065.
  • Belhan S, Çomaklı S, Küçükler S, Gülyüz F, Yıldırım S, Yener Z. Effect of chrysin on methotrexate-induced testicular damage in rats. Andrologia. 2019; 51(1): e13145. DOI: 10.1111/and.13145.
  • Baykalir BG, Arslan AS, Mutlu SI, et al. The protective effect of chrysin against carbon tetrachloride-induced kidney and liver tissue damage in rats. Int J Vitam Nutr Res. 2021; 91(5-6): 427-438. DOI: 10.1024/0300-9831/a000653.
  • Rapa SF, Magliocca G, Pepe G, et al. Protective effect of pomegranate on oxidative stress and inflammatory response induced by 5-fluorouracil in human keratinocytes. Antioxidants (Basel). 2021; 10(2): 203. DOI: 10.3390/antiox10020203.
  • Kucukler S, Benzer F, Yildirim S, et al. Protective effects of chrysin against oxidative stress and inflammation induced by lead acetate in rat kidneys: A biochemical and histopathological approach. Biol Trace Elem Res. 2021; 199(4): 1501-1514. DOI: 10.1007/s12011-020-02268-8.
  • Belhan S, Ozkaraca M, Ozdek U, Komuroglu AU. Protective role of chrysin on doxorubicin-induced oxidative stress and DNA damage in rat testes. Andrologia. 2020; 52(9): e13747. DOI: 10.1111/and.13747.
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Details

Primary Language English
Subjects Health Care Administration
Journal Section Research Articles
Authors

Elif Ayazoglu Demir 0000-0001-7188-2176

Ahmet Mentese 0000-0003-2036-5317

Selim Demir 0000-0002-1863-6280

Hatice Küçük 0000-0002-3724-9104

Nihal Türkmen 0000-0002-8913-8692

Yüksel Aliyazıcıoğlu 0000-0001-9474-4307

Publication Date March 30, 2023
Submission Date November 30, 2022
Published in Issue Year 2023 Volume: 2 Issue: 1

Cite

AMA Ayazoglu Demir E, Mentese A, Demir S, Küçük H, Türkmen N, Aliyazıcıoğlu Y. Evaluation of Therapeutic Effect of Chrysin Against 5-Fluorouracil-Induced Ovarian Damage in Rats. Farabi Med J. March 2023;2(1):1-7.
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