Food and Health
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ANTIBACTERIAL ACTIVITY OF ROYAL JELLY AND RAPE HONEY AGAINST METHICILLIN-RESISTANT Staphylococcus aureus STRAINS

Year 2016, Volume 2, Issue 2, 67 - 73, 10.02.2016

Dinko DİNKOV Deyan STRATEV Ralitsa BALKANSKA Daniel SERGELİDİS

https://doi.org/10.3153/JFHS16007

Abstract

Antimicrobial resistance of pathogenic bacteria is a growing public health problem. Methicillin-resistant Staphylococcus aureus (MRSA) represents a significant burden on healthcare services because it is involved in severe difficult to treat infections in humans. Several non-antibiotic approaches regarding the treatment of infections caused by MRSA as well as of other resistant bacteria have been studied. Since ancient times royal jelly and honey have been considered both as foods and medicines, and recently have been identified as potential alternative medicines to treat bacterial infections, especially of the skin and soft tissue. The aim of the present study was to investigate the antibacterial effect of honey, royal jelly and their mixtures against MRSA in vitro. At least 3 decimal reductions of MRSA count were observed in Tryptone Soy broth with concentrations of 40% rape honey (RP), 20% and 30% royal jelly (RJ), 30% RJ:RH (1:100). In general, honey and royal jelly, individually or in combination, appeared to have a potential as alternative therapeutic agents against MRSA infections, but clinical studies are needed for confirmation.

Keywords

Honey, Royal jelly, Antibacterial activity, MRSA

References

  • Adams, C.J., Boult, C.H., Deadman, B.J., Farr, J.M., Grainger, M.N., Manley-Harris, M. & Snow, M.J. (2008). Isolation by HPLC and characterisation of the bioactive fraction of New Zealand manuka (Leptospermum scoparium) honey. Carbohydrate Research, 343(4), 651-659. doi: 10.1016/j.carres.2007.12.011
  • Alreshoodi, F.M. & Sultanbawa, Y. (2015). Antimicrobial Activity of Royal Jelly. Anti-Infective Agents, 13(1), 50-59. doi: 10.2174/2211352513666150318234430
  • Alzahrani, H.A., Alsabehi, R., Boukraa, L., Abdellah, F., Bellik, Y. & Bakhotmah, B.A. (2012). Antibacterial and Antioxidant Potency of Floral Honeys from Different Botanical and Geographical Origins. Molecules, 17(9), 10540-10549. doi: 10.3390/molecules170910540
  • Balkanska, R., Zhelyazkova, I. & Ignatova, M. (2012). Physico-chemical quality characteristics of royal jelly from three regions of Bulgaria. Agricultural Science and Technology, 4(3), 302-305.
  • Bilikova, K., Huang, S.C., Lin, I.P., Simuth, J. & Peng, C.C. (2015). Structure and antimicrobial activity relationship of royalisin, an antimicrobial peptide from royal jelly of Apis mellifera. Peptides, 68, 190-196. doi: 0.1016/j.peptides.2015.03.001
  • Blum, M.S., Novak, A.F. & Taber, S. (1959). 10-Hydroxy-A2-decenoic acid, an antibiotic found in royal jelly. Science, 130(3373), 452-¬453. doi: 10.1126/science.130.3373.452
  • Bogdanov, S. (1997). Nature and origin of the antibacterial substances in honey. LWT-Food Science and Technology, 30(7), 748-753. doi:10.1006/fstl.1997.0259
  • Bogdanov, S., Martin, P. & Lullman, C. (1997). Harmonized methods of the European Honey Commission. Apidologie (Extra Issue), 1-59.
  • Boukraa, L., Meslem, A., Benhanifia, M. & Hammoudi, S.M. (2009). Synergistic effect of starch and royal jelly against Staphylococcus aureus and Escherichia coli. The Journal of Alternative and Complementary Medicine, 15(7), 755-757. doi: 10.1089/acm.2008.0483
  • Boukraa, L., Niar, A., Benbarek, H. & Benhanifia M. (2008). Additive Action of Royal Jelly and Honey Against Staphylococcus aureus. Journal of Medicinal Food, 11(1), 190-192. doi: 10.1089/jmf.2007.567
  • Brady, N.F., Molan, P.C. & Harfoot, C.G. (1996). The sensitivity of dermatophytes of manuka honey and other honey. Pharmacy and Pharmacology Communications, 2(10), 471-473. doi: 10.1111/j.2042-7158.1996.tb00540.x
  • Cooper, R.A., Halas, E. & Molan, P.C. (2002). The efficacy of honey in inhibiting strains of Pseudomonas aeruginosa from infected burns. Journal of Burn Care & Rehabilitation, 23(6), 366-370.
  • Dinkov, D., Stratev, D. & Balkanska, R. (2014). In vitro atibacterial activity of royal gelly against pathogen Escherichia coli. Veterinary Journal of Republic of Srpska (Banja Luka), 14(1), 14-25. doi: 10.7251/VETJ1401002D.
  • Dustmann, J.H. (1979). Antibacterial effect of honey. Apiacta, 14(1), 7-11.
  • Fontana, R., Mendes, M.A., De Souza, B.M., Cono, K., Cesar, L.M., Maspina, O. & Palma, M.S. (2004). Jelleines: a family of antimicrobial peptides from the Royal Jelly of honeybees (Apis mellifera). Peptides, 25(6), 919-928. doi: 10.1016/j.peptides.2004.03.016
  • Fujiwara, S., Imai, J., Fujiwara, M., Yaeshima, T., Kawashima, T. & Kobayashi, K. (1990). A potent antibacterial protein in royal jelly. Purification and determination of the primary structure of royalisin. The Journal of Biological Chemistry, 265(19), 11333-11337.
  • Kujumgiev, A., Tsvetkova, I., Serkedjieva, Yu., Bankova, V., Christov, R. & Popov, S. (1999). Antibacterial, Antifungal and Antiviral Activity of Propolis of Different Geographic Origin. Journal of Ethnopharmacology, 64(3), 235-240. doi: 10.1016/S0378-8741(98)00131-7
  • Marcucci, M.C., Ferreres, F., Garcia-Viguera, C., Bankova, V.S., De Castro, S.L., Dantas, A. P., Valente, P.H.M. & Paulino, N. (2001). Phenolic compounds from Brazilian propolis with pharmacological activities. Journal of Ethnopharmacology, 74(2), 105-112. doi: 10.1016/S0378-8741(00)00326-3
  • Mavric, E., Wittmann, S., Barth, G. & Henle, T. (2008). Identification and quantification of methylglyoxal as the dominant antibacterial constituent of Manuka (Leptospermum scoparium) honeys from New Zealand. Molecular Nutrition & Food Research, 52(4), 483-489. doi: 10.1002/mnfr.200700282
  • Melliou, E. & Chinou, I. (2005). Chemistry and bioactivity of royal jelly from Greece. Journal of Agricultural and Food Chemistry, 53(23), 8987-8992. doi: 10.1021/jf051550p
  • Molan, P.C. (1992). The antibacterial activity of honey: 1. The nature of the antibacterial activity. Bee World, 73(1), 5-28. http://hdl.handle.net/10289/2094
  • Molan, P.C. (1997). Honey as an antimicrobial agent. In A. Mizrahi & Y. Lensky (Eds.), Bee Products. Properties, Applications, and Apitherapy (pp. 27-37). New York, Springer: Science+Business Media.
  • Oddo, L.P., Piana, L., Bogdanov, S., Bentabol, A., Gotsiou, P., Kerkvied, J., Martin, P., Morlot, M., Valbuena, A.O., Ruoff, K. & von der Ohe, K. (2004). Botanitical species giving unifloral honey in Europe. Apidologie, 35, 82-93. doi: 10.1051/apido:2004045
  • Patton, T., Barrett, J., Brennan, J. & Moran, N. (2006). Use of a spectrophotometric bioassay for determination of microbial sensitivity to manuka honey. Journal of Microbiological Methods, 64(1), 84-95. doi: 10.1016/j.mimet.2005.04.007
  • Sesta, G. (2006). Determination of sugars in royal jelly by HPLC. Apidologie, 37(1), 84-90. doi: 10.1051/apido:2005061
  • Sherlock, O., Dolan, A., Athman, R., Power, A., Gethin, G., Cowman, S. & Humphreys, H. (2010). Comparison of the antimicrobial activity of Ulmo honey from Chile and Manuka honey against methicillin-resistant Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. BMC Complementary and Alternative Medicine, 10: 47. doi: 10.1186/1472-6882-10-47
  • Stratev, D., Vashin, I., Balkanska, R. & Dinkov, D. (2015). Antibacterial activity of royal jelly and rape honey against Aeromonas hydrophila (ATCC 7965). Journal of Food and Health Science, 1(2), 64-74. doi: 10.3153/JFHS15006
  • Taormina, P. J., Niemira, B. A. & Beuchat, L. R. (2001). Inhibitory activity of honey against food-borne pathogens as influenced by the food-borne pathogens as influenced by the presence of hydrogen peroxide and level of antioxidant power. International Journal of Food Microbiology, 69(3), 217-225. doi: 10.1016/S0168-1605(01)00505-0
  • von der Ohe, W., Piana, M. L., Morlot, M. & Martin, P. (2004). Harmonized methods of melissopalynology. Apidologie, 35, 18-25. doi: 10.1051/apido:2004050
  • WHO (World Health Organization). (2014). Antimicrobial resistance: Global Report on Surveillance. Retrieved from http://apps.who.int/iris/bitstream/10665/112642/1/9789241564748_eng.pdf

Year 2016, Volume 2, Issue 2, 67 - 73, 10.02.2016

Dinko DİNKOV Deyan STRATEV Ralitsa BALKANSKA Daniel SERGELİDİS

https://doi.org/10.3153/JFHS16007

Abstract

References

  • Adams, C.J., Boult, C.H., Deadman, B.J., Farr, J.M., Grainger, M.N., Manley-Harris, M. & Snow, M.J. (2008). Isolation by HPLC and characterisation of the bioactive fraction of New Zealand manuka (Leptospermum scoparium) honey. Carbohydrate Research, 343(4), 651-659. doi: 10.1016/j.carres.2007.12.011
  • Alreshoodi, F.M. & Sultanbawa, Y. (2015). Antimicrobial Activity of Royal Jelly. Anti-Infective Agents, 13(1), 50-59. doi: 10.2174/2211352513666150318234430
  • Alzahrani, H.A., Alsabehi, R., Boukraa, L., Abdellah, F., Bellik, Y. & Bakhotmah, B.A. (2012). Antibacterial and Antioxidant Potency of Floral Honeys from Different Botanical and Geographical Origins. Molecules, 17(9), 10540-10549. doi: 10.3390/molecules170910540
  • Balkanska, R., Zhelyazkova, I. & Ignatova, M. (2012). Physico-chemical quality characteristics of royal jelly from three regions of Bulgaria. Agricultural Science and Technology, 4(3), 302-305.
  • Bilikova, K., Huang, S.C., Lin, I.P., Simuth, J. & Peng, C.C. (2015). Structure and antimicrobial activity relationship of royalisin, an antimicrobial peptide from royal jelly of Apis mellifera. Peptides, 68, 190-196. doi: 0.1016/j.peptides.2015.03.001
  • Blum, M.S., Novak, A.F. & Taber, S. (1959). 10-Hydroxy-A2-decenoic acid, an antibiotic found in royal jelly. Science, 130(3373), 452-¬453. doi: 10.1126/science.130.3373.452
  • Bogdanov, S. (1997). Nature and origin of the antibacterial substances in honey. LWT-Food Science and Technology, 30(7), 748-753. doi:10.1006/fstl.1997.0259
  • Bogdanov, S., Martin, P. & Lullman, C. (1997). Harmonized methods of the European Honey Commission. Apidologie (Extra Issue), 1-59.
  • Boukraa, L., Meslem, A., Benhanifia, M. & Hammoudi, S.M. (2009). Synergistic effect of starch and royal jelly against Staphylococcus aureus and Escherichia coli. The Journal of Alternative and Complementary Medicine, 15(7), 755-757. doi: 10.1089/acm.2008.0483
  • Boukraa, L., Niar, A., Benbarek, H. & Benhanifia M. (2008). Additive Action of Royal Jelly and Honey Against Staphylococcus aureus. Journal of Medicinal Food, 11(1), 190-192. doi: 10.1089/jmf.2007.567
  • Brady, N.F., Molan, P.C. & Harfoot, C.G. (1996). The sensitivity of dermatophytes of manuka honey and other honey. Pharmacy and Pharmacology Communications, 2(10), 471-473. doi: 10.1111/j.2042-7158.1996.tb00540.x
  • Cooper, R.A., Halas, E. & Molan, P.C. (2002). The efficacy of honey in inhibiting strains of Pseudomonas aeruginosa from infected burns. Journal of Burn Care & Rehabilitation, 23(6), 366-370.
  • Dinkov, D., Stratev, D. & Balkanska, R. (2014). In vitro atibacterial activity of royal gelly against pathogen Escherichia coli. Veterinary Journal of Republic of Srpska (Banja Luka), 14(1), 14-25. doi: 10.7251/VETJ1401002D.
  • Dustmann, J.H. (1979). Antibacterial effect of honey. Apiacta, 14(1), 7-11.
  • Fontana, R., Mendes, M.A., De Souza, B.M., Cono, K., Cesar, L.M., Maspina, O. & Palma, M.S. (2004). Jelleines: a family of antimicrobial peptides from the Royal Jelly of honeybees (Apis mellifera). Peptides, 25(6), 919-928. doi: 10.1016/j.peptides.2004.03.016
  • Fujiwara, S., Imai, J., Fujiwara, M., Yaeshima, T., Kawashima, T. & Kobayashi, K. (1990). A potent antibacterial protein in royal jelly. Purification and determination of the primary structure of royalisin. The Journal of Biological Chemistry, 265(19), 11333-11337.
  • Kujumgiev, A., Tsvetkova, I., Serkedjieva, Yu., Bankova, V., Christov, R. & Popov, S. (1999). Antibacterial, Antifungal and Antiviral Activity of Propolis of Different Geographic Origin. Journal of Ethnopharmacology, 64(3), 235-240. doi: 10.1016/S0378-8741(98)00131-7
  • Marcucci, M.C., Ferreres, F., Garcia-Viguera, C., Bankova, V.S., De Castro, S.L., Dantas, A. P., Valente, P.H.M. & Paulino, N. (2001). Phenolic compounds from Brazilian propolis with pharmacological activities. Journal of Ethnopharmacology, 74(2), 105-112. doi: 10.1016/S0378-8741(00)00326-3
  • Mavric, E., Wittmann, S., Barth, G. & Henle, T. (2008). Identification and quantification of methylglyoxal as the dominant antibacterial constituent of Manuka (Leptospermum scoparium) honeys from New Zealand. Molecular Nutrition & Food Research, 52(4), 483-489. doi: 10.1002/mnfr.200700282
  • Melliou, E. & Chinou, I. (2005). Chemistry and bioactivity of royal jelly from Greece. Journal of Agricultural and Food Chemistry, 53(23), 8987-8992. doi: 10.1021/jf051550p
  • Molan, P.C. (1992). The antibacterial activity of honey: 1. The nature of the antibacterial activity. Bee World, 73(1), 5-28. http://hdl.handle.net/10289/2094
  • Molan, P.C. (1997). Honey as an antimicrobial agent. In A. Mizrahi & Y. Lensky (Eds.), Bee Products. Properties, Applications, and Apitherapy (pp. 27-37). New York, Springer: Science+Business Media.
  • Oddo, L.P., Piana, L., Bogdanov, S., Bentabol, A., Gotsiou, P., Kerkvied, J., Martin, P., Morlot, M., Valbuena, A.O., Ruoff, K. & von der Ohe, K. (2004). Botanitical species giving unifloral honey in Europe. Apidologie, 35, 82-93. doi: 10.1051/apido:2004045
  • Patton, T., Barrett, J., Brennan, J. & Moran, N. (2006). Use of a spectrophotometric bioassay for determination of microbial sensitivity to manuka honey. Journal of Microbiological Methods, 64(1), 84-95. doi: 10.1016/j.mimet.2005.04.007
  • Sesta, G. (2006). Determination of sugars in royal jelly by HPLC. Apidologie, 37(1), 84-90. doi: 10.1051/apido:2005061
  • Sherlock, O., Dolan, A., Athman, R., Power, A., Gethin, G., Cowman, S. & Humphreys, H. (2010). Comparison of the antimicrobial activity of Ulmo honey from Chile and Manuka honey against methicillin-resistant Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. BMC Complementary and Alternative Medicine, 10: 47. doi: 10.1186/1472-6882-10-47
  • Stratev, D., Vashin, I., Balkanska, R. & Dinkov, D. (2015). Antibacterial activity of royal jelly and rape honey against Aeromonas hydrophila (ATCC 7965). Journal of Food and Health Science, 1(2), 64-74. doi: 10.3153/JFHS15006
  • Taormina, P. J., Niemira, B. A. & Beuchat, L. R. (2001). Inhibitory activity of honey against food-borne pathogens as influenced by the food-borne pathogens as influenced by the presence of hydrogen peroxide and level of antioxidant power. International Journal of Food Microbiology, 69(3), 217-225. doi: 10.1016/S0168-1605(01)00505-0
  • von der Ohe, W., Piana, M. L., Morlot, M. & Martin, P. (2004). Harmonized methods of melissopalynology. Apidologie, 35, 18-25. doi: 10.1051/apido:2004050
  • WHO (World Health Organization). (2014). Antimicrobial resistance: Global Report on Surveillance. Retrieved from http://apps.who.int/iris/bitstream/10665/112642/1/9789241564748_eng.pdf

Details

Subjects Science
Journal Section Articles
Authors

Dinko DİNKOV This is me
Department of Food Hygiene and Control, Veterinary Legislation and Management, Faculty of Veterinary Medicine, Trakia University
Bulgaria

Deyan STRATEV
Department of Food Hygiene and Control, Veterinary Legislation and Management, Faculty of Veterinary Medicine, Trakia University
Bulgaria

Ralitsa BALKANSKA This is me
Department of Special Branches - Bees, Institute of Animal Science
Bulgaria

Daniel SERGELİDİS This is me
School of Veterinary Medicine, Aristotle University of Thessaloniki
Greece

Publication Date February 10, 2016
Application Date January 13, 2015
Acceptance Date February 9, 2016
Published in Issue Year 2016, Volume 2, Issue 2

Cite

Bibtex @research article { jfhs289824, journal = {Food and Health}, eissn = {2602-2834}, address = {Vidin Caddesi No:28 D:4 Kocamustafapaşa 34107 Fatih İstanbul}, publisher = {Özkan ÖZDEN}, year = {2016}, volume = {2}, number = {2}, pages = {67 - 73}, doi = {10.3153/JFHS16007}, title = {ANTIBACTERIAL ACTIVITY OF ROYAL JELLY AND RAPE HONEY AGAINST METHICILLIN-RESISTANT Staphylococcus aureus STRAINS}, key = {cite}, author = {Dinkov, Dinko and Stratev, Deyan and Balkanska, Ralitsa and Sergelidis, Daniel} }
APA Dinkov, D. , Stratev, D. , Balkanska, R. & Sergelidis, D. (2016). ANTIBACTERIAL ACTIVITY OF ROYAL JELLY AND RAPE HONEY AGAINST METHICILLIN-RESISTANT Staphylococcus aureus STRAINS . Food and Health , 2 (2) , 67-73 . DOI: 10.3153/JFHS16007
MLA Dinkov, D. , Stratev, D. , Balkanska, R. , Sergelidis, D. "ANTIBACTERIAL ACTIVITY OF ROYAL JELLY AND RAPE HONEY AGAINST METHICILLIN-RESISTANT Staphylococcus aureus STRAINS" . Food and Health 2 (2016 ): 67-73 <https://jfhs.scientificwebjournals.com/en/pub/issue/27549/289824>
Chicago Dinkov, D. , Stratev, D. , Balkanska, R. , Sergelidis, D. "ANTIBACTERIAL ACTIVITY OF ROYAL JELLY AND RAPE HONEY AGAINST METHICILLIN-RESISTANT Staphylococcus aureus STRAINS". Food and Health 2 (2016 ): 67-73
RIS TY - JOUR T1 - ANTIBACTERIAL ACTIVITY OF ROYAL JELLY AND RAPE HONEY AGAINST METHICILLIN-RESISTANT Staphylococcus aureus STRAINS AU - Dinko Dinkov , Deyan Stratev , Ralitsa Balkanska , Daniel Sergelidis Y1 - 2016 PY - 2016 N1 - doi: 10.3153/JFHS16007 DO - 10.3153/JFHS16007 T2 - Food and Health JF - Journal JO - JOR SP - 67 EP - 73 VL - 2 IS - 2 SN - -2602-2834 M3 - doi: 10.3153/JFHS16007 UR - https://doi.org/10.3153/JFHS16007 Y2 - 2016 ER -
EndNote %0 Food and Health ANTIBACTERIAL ACTIVITY OF ROYAL JELLY AND RAPE HONEY AGAINST METHICILLIN-RESISTANT Staphylococcus aureus STRAINS %A Dinko Dinkov , Deyan Stratev , Ralitsa Balkanska , Daniel Sergelidis %T ANTIBACTERIAL ACTIVITY OF ROYAL JELLY AND RAPE HONEY AGAINST METHICILLIN-RESISTANT Staphylococcus aureus STRAINS %D 2016 %J Food and Health %P -2602-2834 %V 2 %N 2 %R doi: 10.3153/JFHS16007 %U 10.3153/JFHS16007
ISNAD Dinkov, Dinko , Stratev, Deyan , Balkanska, Ralitsa , Sergelidis, Daniel . "ANTIBACTERIAL ACTIVITY OF ROYAL JELLY AND RAPE HONEY AGAINST METHICILLIN-RESISTANT Staphylococcus aureus STRAINS". Food and Health 2 / 2 (February 2016): 67-73 . https://doi.org/10.3153/JFHS16007
AMA Dinkov D. , Stratev D. , Balkanska R. , Sergelidis D. ANTIBACTERIAL ACTIVITY OF ROYAL JELLY AND RAPE HONEY AGAINST METHICILLIN-RESISTANT Staphylococcus aureus STRAINS. Food Health. 2016; 2(2): 67-73.
Vancouver Dinkov D. , Stratev D. , Balkanska R. , Sergelidis D. ANTIBACTERIAL ACTIVITY OF ROYAL JELLY AND RAPE HONEY AGAINST METHICILLIN-RESISTANT Staphylococcus aureus STRAINS. Food and Health. 2016; 2(2): 67-73.
IEEE D. Dinkov , D. Stratev , R. Balkanska and D. Sergelidis , "ANTIBACTERIAL ACTIVITY OF ROYAL JELLY AND RAPE HONEY AGAINST METHICILLIN-RESISTANT Staphylococcus aureus STRAINS", Food and Health, vol. 2, no. 2, pp. 67-73, Feb. 2016, doi:10.3153/JFHS16007
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