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A comparison of Activating Solutions with Hatchery Water in Artificial Insemination of Rainbow Trout (Oncorhynchus mykiss)

Yıl 2018, Cilt: 3 Sayı: 2, 8 - 15, 31.08.2018
https://doi.org/10.31797/vetbio.396372

Öz

In the propagation of rainbow
trout, the available water supply used for incubation of fertilized egg is
generally used for also spermatozoa being activated to reach eggs. The aim of
the present study was to assess comparisons of the effects of two lab-made
activating solutions and hatchery water on progressive sperm motility
percentage (%), duration of progressive sperm motility (s), and fertilization
success in artificial insemination of rainbow trout. For this purpose, an
activating solution (A1) containing 60 mM NaHCO3, 50 mM Tris pH=9.0 and
another activating solution (A2) containing 20 mM Tris, 30 mM glycine, 125 mM
NaCl, pH=9.0, and also  hatchery water
(HW) were used for activation of spermatozoa and fertilization. The average
motility percentages of samples activated by HW, A1 and A2 were observed
>90% with no significant differences, while the durations of progressive
motility were found to be significantly different as 22.5±0.7 s, 30.0±1.4 s and
30.5±0.7 s respectively. The lowest average fertilization rate (64.6±1.4 %) was
obtained using HW, while those values were 89.4±5.1 and 91.3±0.6 % using A1 and
A2 respectively. Consequently, both motility durations and fertilization rates
obtained by using A1 and A2 were significantly higher than those values of
obtained by HW.

Kaynakça

  • Abascal, F.J., Cosson, J., Fauvel, C. (2007). Characterization of sperm motility in sea bass: the effect of heavy metals and physicochemical variables on sperm motility. Journal of Fish Biology, 70(2), 509–522.
  • Alavi, S.M.H., Cosson, J., (2005). Sperm motility in fishes. I. Effects of temperature and pH: a review. Cell Biology International, 29: 101–110.
  • Alavi, S.M.H., Cosson, J. (2006). Sperm motility in fishes. (II) Effects of ions and osmolality: A review. Cell Biology International, 30: 1–14.
  • Billard, R. (1977). A new technique of artificial insemination for salmonids using a sperm diluent. Fisheries, 1: 24–25.
  • Billard, R. (1986). Spermatogenesis and spermatology of some teleost fish species. Reprod Nutr Develop, 26(4): 877–920.
  • Billard, R. (1988). Artificial insemination and gamete management in fish. Marine Behaviour and Physiology, 14(1): 3–21.
  • Billard, R. (1992). Reproduction in rainbow trout: sex differentiation, dynamics of gametogenesis, biology and preservation of gametes. Aquaculture, 100: 263–298.
  • Billard, R., Cosson. J., Perchec, G., Linhart, O. (1995). Biology of sperm and artificial reproduction in carp. Aquaculture, 129: 95–112.
  • Billard, R., Jensen, J.O.T. (1996). Gamete removal, fertilization and incubation. In: Pennell W, Barton BA (ed’s), Principles of Salmonid Culture, Elsevier Science, Amsterdam, the Netherlands, 291-363. 1039 p.
  • Borges, A., Siqueira, S.R., Jurinitz, D.F., Zanini, R., Amaral, F., Grillo, M.L., Oberst, E.R., Wassermann, G.F. (2005). Biochemical composition of seminal plasma and annual variations in semen characteristics of jun-dia Rhamdia quelen (Quoy and Gaimard, Pimelodidae). Fish Physiol Biochem, 31: 45–53.
  • Cejko, B.I., Glogowski, J., Kowalski, R.K., Kucharczyk, D., Targoñska, K. (2008). Description of pikeperch, Sander lucioperca (l.), semen obtained from males held under different rearing conditions. Archives of Polish Fisheries, 16(1): 93–100.
  • Cejko, B.I., Sarosiek, B., Kowalski, R.K. (2013). Application of Computer-assisted Sperm Analysis in Selecting the Suitable Solution for Common Carp, Cyprinus carpio L., Sperm Motility. Journal of the World Aquaculture Society, 44(3): 466–472.
  • Cosson, J. (2004). The ionic and osmotic factors controlling motility of fish spermatozoa. Aquaculture International, 12: 69–85.
  • Cosson, M.P., Cosson, J. Andre, F., Billard, R., (1995). cAMP/ATP relationship in the activation of trout sperm motility: their interaction in membrane-derived models and in live spermatozoa. Cell Motility and the Cytoskeleton, 31:159–176.
  • Gallegoa, V., Peñaranda, D.S., Marco-Jiménez, F., Mazzeo, I., Pérez, L., Asturianoa, J.F. (2012). Comparison of two techniques for the morphometry study on gilthead seabream (Sparus aurata) spermatozoa and evaluation of changes induced by cryopreservation. Theriogenology, 77: 1078–1087.
  • Geffen, A.J., Evans, J.P. (2000). Sperm traits and fertilization success of male and sex-reversed female rainbow trout (Oncorhynchus mykiss). Aquaculture, 182. 61–72.
  • Gwo, J.C. (1994). Cryopreservatıon of yellowfın seabream (Acanthopaqrus latus) spermatozoa (Teleost, Perciformes, Sparidae). Theriogenology, 41: 989-1004.
  • Horváth, A., Miskolczi, E., Urbanyi, B., (2003). Cryopreservation of common carp sperm. Aquat Living Resour, 16: 457–460.
  • Ingermann, R.L., Schultz, C.L.F., Kanuga, M.K., Wilson-Leedy, J.G. (2011). Metabolism of motile zebrafish sperm. Comparative Biochemistry and Physiology Part A, 158: 461-467.
  • Jarmolowicz, S., Demska-Zakes, K., Kowalski, R., Cejko, B.I., Glogowski, J., Zakes, Z. (2010). Impact of dibutyl phthalate and benzyl butyl phthalate on motility parameters of sperm from the European pikeperch Sander lucioperca (L.). Arch Pol Fish, 18: 149–156.
  • Kime, D.E., Van Look, K.J.W., McAllister, B.G., Huyskens, G., Rurangwab, E., Ollevier, F. (2001). Computer-assisted sperm analysis CASA/ as a tool for monitoring sperm quality in fish. Comparative Biochemistry and Physiology Part C, 130: 425–433.
  • Kucharczyk, D.K., Targonska, P., Gomułka, H.P., Kwiatkowski, M., Perkowski, J. (2008). Reproductive parameters of common carp (Cyprinus carpio L.) spawners during natural season and out-of-season spawning. Reproductive Biology, 8(3): 285–289.
  • Lahnsteiner, F. (2000). Semen cryopreservation in the Salmonidae and in the Northern pike. Aqua Res, 31, 245–258.
  • Lahnsteiner, F., Berger, B., Weismann, T. (1999). Sperm Metabolism of the Teleost Fishes Chalcalburnus chalcoides and Oncorhynchus mykis and Its Relation to Motility and Viability. Journal of Experimental Zoology, 284: 454–465.
  • Lahnsteiner, F., Berger, B., Weismann, T., Patzner, R.A. (1996). Motility of spermatozoa of Alburnus alburnus (Cyprinidae) and its relationship to seminal plasma composition and sperm metabolism. Fish Physiology and Biochemistry, 15:167–179.
  • Lahnsteiner, F., Patzner, R.A. (1998). Sperm motility of the marine teleosts Boops boops, Diplodus sargus, Mullus barbatus, and Trachurus mediterraneus. Journal of Fish Biology, 52: 726–742.
  • Li, P., Hulak, M., Li, ZH., Sulc, M., Psenicka, M., Rodina, M., Gela, D., Linhart, O. (2013). Cryopreservation of common carp (Cyprinus carpio L.) sperm induces protein phosphorylation in tyrosine and threonine residues. Theriogenology, 80(2): 84–89.
  • Linhart, O., Rodina, M., Flajshans, M., Gela, D., Kocour, M. (2005). Cryopreservation of European catfish Silurus glanis sperm: Sperm motility, viability, and hatching success of embryos. Cryobiology, 51: 250–261.
  • Perchec, G., Cosson, M.P., Cosson, J., Jeulin, C., Billard, R. (1996). Morphological and kinetic changes of carp (Cyprinus carpio) spermatozoa after initiation of motility in distilled water. Cell Motility and the Cytoskeleton, 35: 113–120.
  • Robles, V., Cabrita, E., Cunado, S., Herraez, M.P. (2003). Sperm cryopreservation of sex-reversed rainbow trout (Oncorhynchus mykiss): parameters that affect its ability for freezing. Aquaculture, 224 203–212
  • Scott, A.P., Bayne, S.M. (1980). A review of the biology, handling and storage of salmonid spermatozoa. J Fish Biol, 17: 707–739.
  • Ubilla, A., Valdebenito, I. (2011). Use of antioxidants on rainbow trout Oncorhynchus mykiss (Walbaum, 1792) sperm diluent: effects on motility and fertilizing capability. Lat Am J Aquat Res, 39(2): 338–343.
  • Vuthiphandchai, V., Thadsri, I., Nimrat, S. (2009). Chilled storage of walking catfish (Clarias macrocephalus) semen. Aquaculture, 296: 58–64.

Gökkuşağı Alabalığı (Oncorhynchus mykiss) Suni Döllemesinde Aktivasyon Solüsyonları ve Kuluçka Suyunun Karşılaştırılması

Yıl 2018, Cilt: 3 Sayı: 2, 8 - 15, 31.08.2018
https://doi.org/10.31797/vetbio.396372

Öz

Gökkuşağı alabalığı üretimin,
genellikle döllenmiş yumurtaların inkübasyonu için kullanılan mevcut su kaynağı
aynı zamanda, suni döllemede sperm hücrelerinin aktivasyonu içinde kullanılır. Bu
çalışma laboratuvarda hazırlanmış aktivasyon solüsyonlarının ve kuluçka suyunun
doğrusal motilite yüzdesi (%), doğrusal motilite süresi (s) ve döllenme
oranlarına etkilerinin değerlendirilmesini amaçlamıştır. Bu amaçla, biri 60 mM
NaHCO3, 50 mM Tris pH=9,0 içeriğine sahip (A1), diğeri 20 mM Tris,
30 mM glycine, 125 mM NaCl, pH=9.0 içeriğine sahip (A2) iki farklı aktivasyon
solüsyonu ve kuluçka suyu (HW) spermatozoa aktivasyonu ve suni dölleme de
kullanılmıştır. HW, A1 ve A2 ile aktive edilmiş örneklerin ortalama motilite
yüzdeleri, istatistiki fark olmaksızın > %90 olarak gözlenmişken, doğrusal
motilite süreleri, sırasıyla 22.5±0.7 s, 30.0±1.4 s ve 30.5±0.7 s olarak
kaydedilmiş ve önemli derecede fark olduğu bulunmuştur. En düşük ortalama
döllenme oranı (% 64.6±1.4) HW kullanılarak elde edilmişken, bu oranların A1 ve
A2 kullanıldığında %89.4±5.1 ve 91.3±0.6 olarak kaydedilmiştir. Sonuç olarak,
A1 ve A2 kullanılarak elde edilen hem motilite süreleri hem de döllenme
oranları, HW kullanılarak elde edilenlerden önemli derecede yüksek olduğu
belirlenmiştir. 

Kaynakça

  • Abascal, F.J., Cosson, J., Fauvel, C. (2007). Characterization of sperm motility in sea bass: the effect of heavy metals and physicochemical variables on sperm motility. Journal of Fish Biology, 70(2), 509–522.
  • Alavi, S.M.H., Cosson, J., (2005). Sperm motility in fishes. I. Effects of temperature and pH: a review. Cell Biology International, 29: 101–110.
  • Alavi, S.M.H., Cosson, J. (2006). Sperm motility in fishes. (II) Effects of ions and osmolality: A review. Cell Biology International, 30: 1–14.
  • Billard, R. (1977). A new technique of artificial insemination for salmonids using a sperm diluent. Fisheries, 1: 24–25.
  • Billard, R. (1986). Spermatogenesis and spermatology of some teleost fish species. Reprod Nutr Develop, 26(4): 877–920.
  • Billard, R. (1988). Artificial insemination and gamete management in fish. Marine Behaviour and Physiology, 14(1): 3–21.
  • Billard, R. (1992). Reproduction in rainbow trout: sex differentiation, dynamics of gametogenesis, biology and preservation of gametes. Aquaculture, 100: 263–298.
  • Billard, R., Cosson. J., Perchec, G., Linhart, O. (1995). Biology of sperm and artificial reproduction in carp. Aquaculture, 129: 95–112.
  • Billard, R., Jensen, J.O.T. (1996). Gamete removal, fertilization and incubation. In: Pennell W, Barton BA (ed’s), Principles of Salmonid Culture, Elsevier Science, Amsterdam, the Netherlands, 291-363. 1039 p.
  • Borges, A., Siqueira, S.R., Jurinitz, D.F., Zanini, R., Amaral, F., Grillo, M.L., Oberst, E.R., Wassermann, G.F. (2005). Biochemical composition of seminal plasma and annual variations in semen characteristics of jun-dia Rhamdia quelen (Quoy and Gaimard, Pimelodidae). Fish Physiol Biochem, 31: 45–53.
  • Cejko, B.I., Glogowski, J., Kowalski, R.K., Kucharczyk, D., Targoñska, K. (2008). Description of pikeperch, Sander lucioperca (l.), semen obtained from males held under different rearing conditions. Archives of Polish Fisheries, 16(1): 93–100.
  • Cejko, B.I., Sarosiek, B., Kowalski, R.K. (2013). Application of Computer-assisted Sperm Analysis in Selecting the Suitable Solution for Common Carp, Cyprinus carpio L., Sperm Motility. Journal of the World Aquaculture Society, 44(3): 466–472.
  • Cosson, J. (2004). The ionic and osmotic factors controlling motility of fish spermatozoa. Aquaculture International, 12: 69–85.
  • Cosson, M.P., Cosson, J. Andre, F., Billard, R., (1995). cAMP/ATP relationship in the activation of trout sperm motility: their interaction in membrane-derived models and in live spermatozoa. Cell Motility and the Cytoskeleton, 31:159–176.
  • Gallegoa, V., Peñaranda, D.S., Marco-Jiménez, F., Mazzeo, I., Pérez, L., Asturianoa, J.F. (2012). Comparison of two techniques for the morphometry study on gilthead seabream (Sparus aurata) spermatozoa and evaluation of changes induced by cryopreservation. Theriogenology, 77: 1078–1087.
  • Geffen, A.J., Evans, J.P. (2000). Sperm traits and fertilization success of male and sex-reversed female rainbow trout (Oncorhynchus mykiss). Aquaculture, 182. 61–72.
  • Gwo, J.C. (1994). Cryopreservatıon of yellowfın seabream (Acanthopaqrus latus) spermatozoa (Teleost, Perciformes, Sparidae). Theriogenology, 41: 989-1004.
  • Horváth, A., Miskolczi, E., Urbanyi, B., (2003). Cryopreservation of common carp sperm. Aquat Living Resour, 16: 457–460.
  • Ingermann, R.L., Schultz, C.L.F., Kanuga, M.K., Wilson-Leedy, J.G. (2011). Metabolism of motile zebrafish sperm. Comparative Biochemistry and Physiology Part A, 158: 461-467.
  • Jarmolowicz, S., Demska-Zakes, K., Kowalski, R., Cejko, B.I., Glogowski, J., Zakes, Z. (2010). Impact of dibutyl phthalate and benzyl butyl phthalate on motility parameters of sperm from the European pikeperch Sander lucioperca (L.). Arch Pol Fish, 18: 149–156.
  • Kime, D.E., Van Look, K.J.W., McAllister, B.G., Huyskens, G., Rurangwab, E., Ollevier, F. (2001). Computer-assisted sperm analysis CASA/ as a tool for monitoring sperm quality in fish. Comparative Biochemistry and Physiology Part C, 130: 425–433.
  • Kucharczyk, D.K., Targonska, P., Gomułka, H.P., Kwiatkowski, M., Perkowski, J. (2008). Reproductive parameters of common carp (Cyprinus carpio L.) spawners during natural season and out-of-season spawning. Reproductive Biology, 8(3): 285–289.
  • Lahnsteiner, F. (2000). Semen cryopreservation in the Salmonidae and in the Northern pike. Aqua Res, 31, 245–258.
  • Lahnsteiner, F., Berger, B., Weismann, T. (1999). Sperm Metabolism of the Teleost Fishes Chalcalburnus chalcoides and Oncorhynchus mykis and Its Relation to Motility and Viability. Journal of Experimental Zoology, 284: 454–465.
  • Lahnsteiner, F., Berger, B., Weismann, T., Patzner, R.A. (1996). Motility of spermatozoa of Alburnus alburnus (Cyprinidae) and its relationship to seminal plasma composition and sperm metabolism. Fish Physiology and Biochemistry, 15:167–179.
  • Lahnsteiner, F., Patzner, R.A. (1998). Sperm motility of the marine teleosts Boops boops, Diplodus sargus, Mullus barbatus, and Trachurus mediterraneus. Journal of Fish Biology, 52: 726–742.
  • Li, P., Hulak, M., Li, ZH., Sulc, M., Psenicka, M., Rodina, M., Gela, D., Linhart, O. (2013). Cryopreservation of common carp (Cyprinus carpio L.) sperm induces protein phosphorylation in tyrosine and threonine residues. Theriogenology, 80(2): 84–89.
  • Linhart, O., Rodina, M., Flajshans, M., Gela, D., Kocour, M. (2005). Cryopreservation of European catfish Silurus glanis sperm: Sperm motility, viability, and hatching success of embryos. Cryobiology, 51: 250–261.
  • Perchec, G., Cosson, M.P., Cosson, J., Jeulin, C., Billard, R. (1996). Morphological and kinetic changes of carp (Cyprinus carpio) spermatozoa after initiation of motility in distilled water. Cell Motility and the Cytoskeleton, 35: 113–120.
  • Robles, V., Cabrita, E., Cunado, S., Herraez, M.P. (2003). Sperm cryopreservation of sex-reversed rainbow trout (Oncorhynchus mykiss): parameters that affect its ability for freezing. Aquaculture, 224 203–212
  • Scott, A.P., Bayne, S.M. (1980). A review of the biology, handling and storage of salmonid spermatozoa. J Fish Biol, 17: 707–739.
  • Ubilla, A., Valdebenito, I. (2011). Use of antioxidants on rainbow trout Oncorhynchus mykiss (Walbaum, 1792) sperm diluent: effects on motility and fertilizing capability. Lat Am J Aquat Res, 39(2): 338–343.
  • Vuthiphandchai, V., Thadsri, I., Nimrat, S. (2009). Chilled storage of walking catfish (Clarias macrocephalus) semen. Aquaculture, 296: 58–64.
Toplam 33 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Hidrobiyoloji
Bölüm Araştırma Makaleleri
Yazarlar

Burak Evren İnanan 0000-0002-2888-8457

Ümit Acar 0000-0003-2515-5490

Hüseyin Urçuk Bu kişi benim 0000-0002-5850-6018

Ersin Çelik Bu kişi benim 0000-0003-2547-8660

Yayımlanma Tarihi 31 Ağustos 2018
Gönderilme Tarihi 18 Şubat 2018
Kabul Tarihi 30 Temmuz 2018
Yayımlandığı Sayı Yıl 2018 Cilt: 3 Sayı: 2

Kaynak Göster

APA İnanan, B. E., Acar, Ü., Urçuk, H., Çelik, E. (2018). A comparison of Activating Solutions with Hatchery Water in Artificial Insemination of Rainbow Trout (Oncorhynchus mykiss). Journal of Advances in VetBio Science and Techniques, 3(2), 8-15. https://doi.org/10.31797/vetbio.396372

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