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Relationship between Natriuretic Peptide Levels with Heart and Kidney Parameters in Rats with Chronic Renal Failure

Yıl 2018, Cilt: 29 Sayı: 3, 135 - 141, 27.12.2018

Öz

In
the present study, the relationship between heart and kidney parameters
compared to natriuretic peptide levels in rats having experimentally induced
chronic renal failure were aimed to investigate. For this purpose, 60 Wistar
albino rats were used as animal material. These rats were divided into two
groups as control (10 male and 10 female) and study group (20 male and 20
female). Adenin (1gr/L) were added to the drinking water of the study group
during 28 days. On the last day of the experiment, the animals were sacrificed
under anaesthesia and blood, kidney, heart and liver samples were collected
from all animals. When biochemical parameters examined; Ca++ (p<0.01) and P
(p<0.01) values increased in the study group compared to control group, but
AST, ALT, LDH, CK, CK-MB and cTn I levels did not change significantly in both
groups. Creatinin, urea and BUN, which all are diagnostic markers of kidney,
levels increased in the study group as 1.55±0.20 mg/dL, 239.34 ± 22.00 mg/dL
and 123.05 ± 15.02 mg/dL respectively. The same values in the control group
were 0.34 ± 0.24 mg/dL, 41.07 ±1.39 mg/dL and 19.18 ± 0.6 mg/dL respectively.
When natriuretic peptide parameters evaluated; BNP and NT-ProBNP levels
increased significantly in the study group and the values were as 79.10 ± 12.64
pg/ml and 84.45 ±17.31 pg/ml respectively and the same values in the control
group were 37.93 ± 3.68 pg/ml and 40.01 ± 5.85 pg/ml respectively. In the
necropsy, enlargement in kidneys, and fading in their colours were observed in
macroscopic examination. But, macroscopic changes in liver and heart were not
observed in both groups. In the microscopic examination; tubular dilatation,
crystallisation in the intertubular space and giant cell formation,
degenerations in the tubuloseptills and tubulointerstitial nephritis were
observed in the study group. In contrast, in the control group no changes
observed both macroscopically and microscopically. As a result; it was
concluded that diagnostic parameters used in the chronic renal failure may have
a linear relationship between natriuretic peptides (especially BNP and
NT-proBNP).

Kaynakça

  • Akcan AB, Oygür N (2010). Beyin Natriüretik Peptid ve Pediatride Kullanım Alanları. Güncel Pediatri; 8, 67-71Asano Y, Susami M, Honda K, Serikawa T (1998) Haematological and serum biochemical values in spontaneously epileptic malerats and related rat strains.Laboratory Animals. 32, 214-218Ataka K, Maruyama H, Neichi T, Miyazaki JI, Gejyo F (2003). Effects of erythropoietin-gene electrotransfer in rats with adenine-ınduced renal failure. American journal of nephrology. 23, 5, 315-323.Babuin L and Jaffe AS (2005). Review, Troponin: The Biomarker of choice for the detection of cardiac injury. CMAJ 173, 10, 1191-1202Badreldin H Ali, Suhail Al-Salam, Isehaq Al Husseni, Rana R Kayed, Noura Al-Masroori, Thuriya Al-Harthi, Mohamed Al Zaabi and Abderrahim N (2010). Effects of Gum Arabic in rats with adenine-induced chronic renal failure Society for Experimental Biology and Medicine. 235, 373-382.Benjamin MM (1978). Outline of Veterinary Clinical Pathology. Third Ed. Colorado State University. USABuncharoen W, Saenphet S, Chomdej S ve Saenphet K (2012). Evaluation of biochemical, hematological and histopathological parameters of albino rats treated with Stemona aphylla Craib. Extract. Journal of Medicinal Plants Research 6, 27, 4429-4435Connolly DJ, Magalhaes SJR, Syme MH, Boswood A, Fuentes LV, Chu L, Metcalf M (2008) Circulating Natriuretic Peptides in Cat with Heart Disease. J. Vet Intern Med. 22, 96-105Çakır Z, Sarıtaş A, Aslan Ş (2006). Brain Natriüretik peptid acil serviste kulanı alanı ve önemi.The Eurasian journal of medicine, 38, 130-133.Çam N, Vural M (2003). Kalp Yetersizliği Tanı, Tedavi ve Prognozunun Belirlenmesinde B-tipi Natriüretik Peptidin Yeri. Türk kardiol Der. Arş. 31, 8, 458-465.Damment S, Secker R, Shen V, Lorenzo V. and Rodriguez M.(2010). Long-term treatment with lanthanum carbonate reduces mineral and bone abnormalities in rats with chronic renal failure. Nephrol Dial Transplant. 26, 1803-1812.Duygu H, Türk U, Zoghi M, Nalbantgil S (2005). Plazma B-Tipi Natriüretik Peptid Düzeylerinin Kardiyovasküler Hastalıklardaki Yeri ve Önemi. Anadolu Kardiyal Derg. 5, 305-311Elmalı E, Karaeren Z, Özgül Ç ve Akan ÖA (2005). Akut Koroner Sendrom Şüpheli Hastalarda Kardiak Troponin T ve Troponin I’nın Karşılaştırılması. Türk Biyokimya Dergisi 30, 3, 212-215Greco DS, Biller B, Liew CH (2003). Measurement of Plasma Atrial Natriuretic Peptideas an Indicator of Prognosis in Dogs with Cardiac Disease. Can Vet J. 44, 293–297.Hori Y, Kikuchi M, Nakagawa A, Yonezawa T, Muira H, Ohnami Y (2009). Plasma Atrial Natriuretic peptid in Healthy Calves and Calves with Congenital Heart Disease. J. Vet Intern Med. 23, 653-656Hür E, Bozkurt D, Timur Ö, Bıçak S, Narlı Z, Erden A, Patır P, Şen S, Akçiçek F, Duman S (2010). Kronik böbrek yetmezliği oluşturma protokolünde pratik bir model. Türkiye Klinikleri Nefroloji özel dergisi. 3, 3, 30-33.Ienaga K, Mikami H ve Yokozawa T (2009). First indications demonstrating the preventive effects of NZ-419, a novel intrinsic antioxidant, on the initiation and/or progression of chronic renal failure in rats. Biol Pharm Bull., 32, 7, 1204-8İde T (2003). Laboratuvar Hayvanları Biliminin Temel İlkeleri. Ankara: Medipres Mat.Kaneko JJ, Harvey JW and Bruss ML (1997). Clinical Biochemistry of Domestic Animals. 50. Ed., Academic Pres., California, USAKlinger JR, Warburton RR, Pietras L ve Hill NS (1998) Brain natriüretic peptide inhibits hypoxic pulmonary hypertension in rats. J Appl Physiol 84, 1646-1652Koç M, Yeğen BÇ (2010) Kronik böbrek yetmezliği modelleri. Turkiye Klinikleri J Nephrol-Special Topics, 3, 3, 23-9.Lacour, B., Lucas, A., Auchère, D., Ruellan, N., Patey, N. M. D. S., & Drüeke, T. B. (2005). Chronic renal failure is associated with increased tissue deposition of lanthanum after 28-day oral administration. Kidney international, 67(3), 1062-1069.Marttila M, Vuolteenaho O, Ganten D, Nakao K, Ruskoaho H (1996). Synthesis and Secretion of Natriuretic Peptides in the Hypertensive TGR(mREN-2)27 Transgenic Rat. Hypertension, ahajournals. 28, 995-1004Matsui I, Hamano T, Mikami S, Fujii N, Takabatake Y, Nagasawa Y, Kawada N, Ito T, Rakugi H, Imai E, Isaka Y. (2009). Fully phosphorylated fetuin-A forms a mineral complex in the serum of rats with adenine-induced renal failure. Kidney Int.;75, 9; 915-28Mueller C, Breidthardt T, Laule-Kilian K, Christ M, Perruchoud AP (2007). The integration of BNP and NT-proBNP into clinical medicine. Swiss Med Wkly. 13;137, 1, 2, 4-12.Mueller C, Laule-Kilian K, Scholer A, Nusbaumer C, Zeller T, Staub D and Perruchoud AP (2005). B-Type Natriuretic Peptide for Acute Dyspnea in Patients with Kidney Disease: Insights from a Randomized Comparison. Kidney International. 67, 278-284.Oyama MA (2009). Neurohormonal Activation in Canine Degenerative Mitral Valve Disease: Implications on Pathophysiology and Treatment. Journal of Small Animal Practice. 50, 3-11.Saad SY, Najjar TA ve Arafah MM (2006b). Cardioprotective effects of subcutaneous ebselen against daunorubicin-induced cardioomyopathy in rats. Basic&Clincal Pharmacology&Toxicology. 99, 412-417San A, Selçuk NY (1993). Atrial Natriüretik peptid. Türk Nefroloji Diyaliz ve Transplantasyon Dergisi, 1, 1-6.Selamet U (2005). Üremik hastalarda Görsel Uyarılmış Potansiyeller ve Elektroretinogram Değişiklikleri. Uzmanlık Tezi. Haydarpaşa numune eğitim ve araştırma hastanesi 2. Dahiliye Kliniği, İstanbulTanrıverdi MH, Karadağ A, ve Hatipoğlu EŞ (2010). Kronik böbrek yetmezliği. (Derleme) Konuralp Tıp Dergisi. 2, 2, 27-32.Turgut K (2000). Veteriner Klinik Laboratuvar Teşhis. Bahçıvanlar Basım Sanayi KonyaYalçın AU, Akpolat T (2013) Kronik bobrek yetmezliği, www. tsn.org.tr/folders/file/kronik_bobrek_yetmezligi.pdf (Erişim 19.01.2013) Zhang ZP, Tian YH, Li R, Cheng XQ, Guo SM, Zhang JX, Wang JJ and Hu L(2004). The comparison of the normal blood biochemical values of Wistar rats with different age and sex. Asian Journal of Drug Metabolism and Pharmacokinetics 4,3; 215-218Zhao, Y. Y., Liu, J., Cheng, X. L., Bai, X., & Lin, R. C. (2012). Urinary metabonomics study on biochemical changes in an experimental model of chronic renal failure by adenine based on UPLC Q-TOF/MS. Clinica chimica acta, 413(5), 642-649.

Kronik Böbrek Hasarı Oluşturulan Ratlarda Kalp ve Böbrek Parametreleri İle Natriüretik Peptid Düzeyleri Arasındaki İlişki

Yıl 2018, Cilt: 29 Sayı: 3, 135 - 141, 27.12.2018

Öz

Bu çalışmada kronik böbrek yetmezliği oluşturulan ratlarda kalp ve böbrek parametreleri ile natriüretik peptid düzeyleri arasındaki ilişkinin ortaya konulması hedeflendi. Bu amaçla 60 adet Wistar albino rat materyal olarak kullanıldı. Ratlar, kontrol (10 dişi-10 erkek) ve çalışma (20 dişi-20 erkek) olmak üzere iki gruba ayrıldı. Çalışma grubunun içme sularına 28 gün boyunca 1gr/L adenin katıldı. Çalışmanın son gününde hayvanlar anestezi altında sakrifiye edilerek, kan, böbrek, kalp ve karaciğer numuneleri alındı. Biyokimyasal parametreler irdelendiğinde; Ca+2 (p<0.01) ve P (p<0.01) düzeylerinde istatistiki olarak kontrol grubuna göre çalışma grubunda artış belirlenmesine rağmen, AST, ALT, LDH, CK, CK-MB ve cTn I değerlerinde kontrol ve çalışma grupları arasında istatistiki olarak önemli bir fark belirlenmedi. Böbrek diagnostik belirteci olan kreatinin, üre ve BUN düzeyleri çalışma grubunda artış göstererek sırasıyla 1.55±0.20 mg/dL, 239.34±22.00 mg/dL, 123.05±15.02 mg/dL düzeyine gelmesine karşın kontrol grubunda ise bu değerler sırasıyla 0.34±0.24 mg/dL, 41.07±1.39, 19.18±0.6 mg/dL seviyesinde kaldığı belirlendi. Natriüretik peptid parametreleri incelendiğinde BNP ve NT-proBNP değerleri çalışma grubunda artış göstererek 79.10±12.64 pg/ml- 84.45±17.31 pg/mldüzeyine geldiği tespit edilmesine karşın kontrol grubunda ise bu değerler 37.93±3.68 pg/ml- 40.01±5.85 pg/mlseviyesinde kaldığı belirlendi. Deneklerin nekropsisinde çalışma grubunun makroskopik bakısında böbreklerde büyüme ve renklerinde açılma gözlenirken, kalp ve karaciğerde herhangi bir değişikliğe rastlanmadı. Mikroskobik bakıda ise böbreklerde tubuler dilatasyon, intertubuler aralıkta kristalizasyon ve dev hücre oluşumu, tubulus epitellerinde dejenerasyon ve tubulointersitisiyel nefritise rastlandı. Buna karşın kontrol grubunun gerek makroskopik gerekse mikroskobik bakısında herhangi bir değişikliğe rastlanmadı. Sonuç olarak kronik böbrek yetmezliğinde kullanılan diagnostik parametrelerle natriüretik peptitler (özellikle BNP ve NT-proBNP)arasında doğrusal bir ilişki olduğu kanısına varıldı.

Kaynakça

  • Akcan AB, Oygür N (2010). Beyin Natriüretik Peptid ve Pediatride Kullanım Alanları. Güncel Pediatri; 8, 67-71Asano Y, Susami M, Honda K, Serikawa T (1998) Haematological and serum biochemical values in spontaneously epileptic malerats and related rat strains.Laboratory Animals. 32, 214-218Ataka K, Maruyama H, Neichi T, Miyazaki JI, Gejyo F (2003). Effects of erythropoietin-gene electrotransfer in rats with adenine-ınduced renal failure. American journal of nephrology. 23, 5, 315-323.Babuin L and Jaffe AS (2005). Review, Troponin: The Biomarker of choice for the detection of cardiac injury. CMAJ 173, 10, 1191-1202Badreldin H Ali, Suhail Al-Salam, Isehaq Al Husseni, Rana R Kayed, Noura Al-Masroori, Thuriya Al-Harthi, Mohamed Al Zaabi and Abderrahim N (2010). Effects of Gum Arabic in rats with adenine-induced chronic renal failure Society for Experimental Biology and Medicine. 235, 373-382.Benjamin MM (1978). Outline of Veterinary Clinical Pathology. Third Ed. Colorado State University. USABuncharoen W, Saenphet S, Chomdej S ve Saenphet K (2012). Evaluation of biochemical, hematological and histopathological parameters of albino rats treated with Stemona aphylla Craib. Extract. Journal of Medicinal Plants Research 6, 27, 4429-4435Connolly DJ, Magalhaes SJR, Syme MH, Boswood A, Fuentes LV, Chu L, Metcalf M (2008) Circulating Natriuretic Peptides in Cat with Heart Disease. J. Vet Intern Med. 22, 96-105Çakır Z, Sarıtaş A, Aslan Ş (2006). Brain Natriüretik peptid acil serviste kulanı alanı ve önemi.The Eurasian journal of medicine, 38, 130-133.Çam N, Vural M (2003). Kalp Yetersizliği Tanı, Tedavi ve Prognozunun Belirlenmesinde B-tipi Natriüretik Peptidin Yeri. Türk kardiol Der. Arş. 31, 8, 458-465.Damment S, Secker R, Shen V, Lorenzo V. and Rodriguez M.(2010). Long-term treatment with lanthanum carbonate reduces mineral and bone abnormalities in rats with chronic renal failure. Nephrol Dial Transplant. 26, 1803-1812.Duygu H, Türk U, Zoghi M, Nalbantgil S (2005). Plazma B-Tipi Natriüretik Peptid Düzeylerinin Kardiyovasküler Hastalıklardaki Yeri ve Önemi. Anadolu Kardiyal Derg. 5, 305-311Elmalı E, Karaeren Z, Özgül Ç ve Akan ÖA (2005). Akut Koroner Sendrom Şüpheli Hastalarda Kardiak Troponin T ve Troponin I’nın Karşılaştırılması. Türk Biyokimya Dergisi 30, 3, 212-215Greco DS, Biller B, Liew CH (2003). Measurement of Plasma Atrial Natriuretic Peptideas an Indicator of Prognosis in Dogs with Cardiac Disease. Can Vet J. 44, 293–297.Hori Y, Kikuchi M, Nakagawa A, Yonezawa T, Muira H, Ohnami Y (2009). Plasma Atrial Natriuretic peptid in Healthy Calves and Calves with Congenital Heart Disease. J. Vet Intern Med. 23, 653-656Hür E, Bozkurt D, Timur Ö, Bıçak S, Narlı Z, Erden A, Patır P, Şen S, Akçiçek F, Duman S (2010). Kronik böbrek yetmezliği oluşturma protokolünde pratik bir model. Türkiye Klinikleri Nefroloji özel dergisi. 3, 3, 30-33.Ienaga K, Mikami H ve Yokozawa T (2009). First indications demonstrating the preventive effects of NZ-419, a novel intrinsic antioxidant, on the initiation and/or progression of chronic renal failure in rats. Biol Pharm Bull., 32, 7, 1204-8İde T (2003). Laboratuvar Hayvanları Biliminin Temel İlkeleri. Ankara: Medipres Mat.Kaneko JJ, Harvey JW and Bruss ML (1997). Clinical Biochemistry of Domestic Animals. 50. Ed., Academic Pres., California, USAKlinger JR, Warburton RR, Pietras L ve Hill NS (1998) Brain natriüretic peptide inhibits hypoxic pulmonary hypertension in rats. J Appl Physiol 84, 1646-1652Koç M, Yeğen BÇ (2010) Kronik böbrek yetmezliği modelleri. Turkiye Klinikleri J Nephrol-Special Topics, 3, 3, 23-9.Lacour, B., Lucas, A., Auchère, D., Ruellan, N., Patey, N. M. D. S., & Drüeke, T. B. (2005). Chronic renal failure is associated with increased tissue deposition of lanthanum after 28-day oral administration. Kidney international, 67(3), 1062-1069.Marttila M, Vuolteenaho O, Ganten D, Nakao K, Ruskoaho H (1996). Synthesis and Secretion of Natriuretic Peptides in the Hypertensive TGR(mREN-2)27 Transgenic Rat. Hypertension, ahajournals. 28, 995-1004Matsui I, Hamano T, Mikami S, Fujii N, Takabatake Y, Nagasawa Y, Kawada N, Ito T, Rakugi H, Imai E, Isaka Y. (2009). Fully phosphorylated fetuin-A forms a mineral complex in the serum of rats with adenine-induced renal failure. Kidney Int.;75, 9; 915-28Mueller C, Breidthardt T, Laule-Kilian K, Christ M, Perruchoud AP (2007). The integration of BNP and NT-proBNP into clinical medicine. Swiss Med Wkly. 13;137, 1, 2, 4-12.Mueller C, Laule-Kilian K, Scholer A, Nusbaumer C, Zeller T, Staub D and Perruchoud AP (2005). B-Type Natriuretic Peptide for Acute Dyspnea in Patients with Kidney Disease: Insights from a Randomized Comparison. Kidney International. 67, 278-284.Oyama MA (2009). Neurohormonal Activation in Canine Degenerative Mitral Valve Disease: Implications on Pathophysiology and Treatment. Journal of Small Animal Practice. 50, 3-11.Saad SY, Najjar TA ve Arafah MM (2006b). Cardioprotective effects of subcutaneous ebselen against daunorubicin-induced cardioomyopathy in rats. Basic&Clincal Pharmacology&Toxicology. 99, 412-417San A, Selçuk NY (1993). Atrial Natriüretik peptid. Türk Nefroloji Diyaliz ve Transplantasyon Dergisi, 1, 1-6.Selamet U (2005). Üremik hastalarda Görsel Uyarılmış Potansiyeller ve Elektroretinogram Değişiklikleri. Uzmanlık Tezi. Haydarpaşa numune eğitim ve araştırma hastanesi 2. Dahiliye Kliniği, İstanbulTanrıverdi MH, Karadağ A, ve Hatipoğlu EŞ (2010). Kronik böbrek yetmezliği. (Derleme) Konuralp Tıp Dergisi. 2, 2, 27-32.Turgut K (2000). Veteriner Klinik Laboratuvar Teşhis. Bahçıvanlar Basım Sanayi KonyaYalçın AU, Akpolat T (2013) Kronik bobrek yetmezliği, www. tsn.org.tr/folders/file/kronik_bobrek_yetmezligi.pdf (Erişim 19.01.2013) Zhang ZP, Tian YH, Li R, Cheng XQ, Guo SM, Zhang JX, Wang JJ and Hu L(2004). The comparison of the normal blood biochemical values of Wistar rats with different age and sex. Asian Journal of Drug Metabolism and Pharmacokinetics 4,3; 215-218Zhao, Y. Y., Liu, J., Cheng, X. L., Bai, X., & Lin, R. C. (2012). Urinary metabonomics study on biochemical changes in an experimental model of chronic renal failure by adenine based on UPLC Q-TOF/MS. Clinica chimica acta, 413(5), 642-649.
Toplam 1 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Veteriner Cerrahi
Bölüm Makaleler
Yazarlar

Yıldıray Basbugan

Zahid Tevfik Ağaoğlu

Yayımlanma Tarihi 27 Aralık 2018
Gönderilme Tarihi 30 Mart 2018
Kabul Tarihi 12 Ekim 2018
Yayımlandığı Sayı Yıl 2018 Cilt: 29 Sayı: 3

Kaynak Göster

APA Basbugan, Y., & Ağaoğlu, Z. T. (2018). Relationship between Natriuretic Peptide Levels with Heart and Kidney Parameters in Rats with Chronic Renal Failure. Van Veterinary Journal, 29(3), 135-141.
AMA Basbugan Y, Ağaoğlu ZT. Relationship between Natriuretic Peptide Levels with Heart and Kidney Parameters in Rats with Chronic Renal Failure. Van Vet J. Aralık 2018;29(3):135-141.
Chicago Basbugan, Yıldıray, ve Zahid Tevfik Ağaoğlu. “Relationship Between Natriuretic Peptide Levels With Heart and Kidney Parameters in Rats With Chronic Renal Failure”. Van Veterinary Journal 29, sy. 3 (Aralık 2018): 135-41.
EndNote Basbugan Y, Ağaoğlu ZT (01 Aralık 2018) Relationship between Natriuretic Peptide Levels with Heart and Kidney Parameters in Rats with Chronic Renal Failure. Van Veterinary Journal 29 3 135–141.
IEEE Y. Basbugan ve Z. T. Ağaoğlu, “Relationship between Natriuretic Peptide Levels with Heart and Kidney Parameters in Rats with Chronic Renal Failure”, Van Vet J, c. 29, sy. 3, ss. 135–141, 2018.
ISNAD Basbugan, Yıldıray - Ağaoğlu, Zahid Tevfik. “Relationship Between Natriuretic Peptide Levels With Heart and Kidney Parameters in Rats With Chronic Renal Failure”. Van Veterinary Journal 29/3 (Aralık 2018), 135-141.
JAMA Basbugan Y, Ağaoğlu ZT. Relationship between Natriuretic Peptide Levels with Heart and Kidney Parameters in Rats with Chronic Renal Failure. Van Vet J. 2018;29:135–141.
MLA Basbugan, Yıldıray ve Zahid Tevfik Ağaoğlu. “Relationship Between Natriuretic Peptide Levels With Heart and Kidney Parameters in Rats With Chronic Renal Failure”. Van Veterinary Journal, c. 29, sy. 3, 2018, ss. 135-41.
Vancouver Basbugan Y, Ağaoğlu ZT. Relationship between Natriuretic Peptide Levels with Heart and Kidney Parameters in Rats with Chronic Renal Failure. Van Vet J. 2018;29(3):135-41.

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