Araştırma Makalesi
Uluabat Gölündeki Tahta (Blicca bjoerkna (L., 1758)) Balığının Mide ve Bağırsak Dokularının Histokimyasal Açıdan Karşılaştırılması
Öz
Yapılan çalışmada tahta (Blicca bjoerkna (L., 1758)) balığının mide ve bağırsak bölümlerinin histolojik ve histokimyasal açıdan karşılaştırılması amaçlanmaktadır. Blicca bjoerkna’nın sindirim sistemi morfolojik olarak; mide, ilk bağırsak, orta bağırsak ve son bağırsak olmak üzere dört bölüme ayrıldı. Çalışılan balıklar ergin olarak Uluabat Gölü’nden temin edildi. Karanfil yağı anestezisi uygulandı. Daha sonra abdominal diseksiyon ile mide ve bağırsaklardan örnek alımı gerçekleştirildi. Alınan örneklerin ışık mikroskobunda morfolojik ve histokimyasal yapısı incelendi. Mukosubstans özellikleri, yoğunlukları ve dağılımlarını göstermek için PAS, PAS/AB pH 2.5, AB pH 2.5, AF, AF/AB pH 2.5 histokimyasal boyamaları uygulandı.
Anahtar Kelimeler
Kaynakça
- Banan-Khojasteh SM, Sheikhzadeh F, Mohammadnejad D, Azami A (2009). Histological, Histochemical and Ultrastructural Study of the Intestine of Rainbow Trout (Oncorhynchus mykiss), World Appl Sci J, 6(11), 1525-1531. Bosi G, Shinn AP, Giari L, Simoni E, Pironi F, Dezfuli BS (2005). Changes in the Neuromodulators of the Diffuse Endocrine System of the Alimentary Canal of Farmed Rainbow Trout. Oncorhynchus mykiss (Walbaum), Naturally Infected with Eubothrium crassum (Cestoda). J Fish Dis, 28, 703-711. Çınar Ş, Küçükkara R, Balık İ, Çubuk H, Ceylan M, Erol K G, Yeğen V, Bulut C (2013). Uluabat (Apolyont) Gölü’ndeki Balık Faunasının Tespiti, Tür Kompozisyonu ve Ticari Avcılığın Türlere Göre Dağılımı. J Fish Sci, 7(4), 309-316. Çınar Ş, Küçükkara R, Ceylan M, Çubuk H, Erol K G, Akçimen U, Savaşer S (2008). Uluabat Gölü’ndeki Kızılkanat (Scardinius erythrophthalmus L.,1758) Populasyonu’nun Büyüme Parametrelerinin Araştırılması. Ege Üniv Su Ürün Derg, 25, (4), 289–293. Desantis S, Acone F, Zizza S, Deflorio M, Fernandez JLP, Sarasquete C, De Metrio G (2009). Glycohistochemical Study of the Toadfish Halobatrachus didactylus (Scheider, 1801) Stomach. Sci Mar, 73(3), 515- 525. Diaz AO, Garcia AM, Devincenti CV, Goldemberg AL (2003). Morphological and Histochemical Characterization of the Mucosa of the Digestive Tract in Engraulis anchoita. Anat Histol Embryol, 32, 341–346. Diaz AO, Garcia AM, Figueroa DE, Goldemberg AL (2007). The Mucosa of Digestive Tract in Micropogonias furnieri: A Light and Electron Microscope Approach, Anat Histol Embryol, 37, 251–256. Gomari (1952). Gomari’s Aldehyde Fuchsin stain. In: Cellular Pathology Technique (C F A Culling, R T Allison and W T Barr, eds). Butterworths, London. Khojasteh SMB, Ghodratiniya S (2013). Gastric and Intestinal Morphohistology of Epinephelus Coioides (Osteichthyes, Serranidae). Int J Aquac Sci, 4 (2), 83-90. Leknes IL (2010). Histochemical Study on the Intestine Goblet Cells in Cichlid and Poecilid Secies (Teleostei). Tissue and Cell, 42, 61-64. Leknes IL (2013). Goblet Cell Types in Intestine of Tiger Barb nd Black Tetra (Cyprinidae, Characidae: Teleostei). Anat Histol Embryol, doi: 10.1111/ahe.12083. Lev R, Spicer SS (1964). Specific staining of sulphate groups with alcian blue at low pH. J Histochem Cytochem, 12, 309 McManus JFA (1948). Histological and Histochemical Uses of Periodic Acid. Stain Technol, 23, 99–108. Mowry RW (1956). Alcian Blue Techniques for the Histochemical Study of Acidic Carbohydrates. J Histochem Cytochem, 4, 407–408. Neuhaus H, Van Der Marel M, Caspari N, Meyeri W, Enss ML, Steinhagen D (2007). Biochemical and Histochemical Study on the Intestinal Mucosa of the Common Carp Cyrprinus carpio L., with Special Consideration of Mucin Glycoproteins. J Fish Biol, 70, 1523-1534. Park JY, Kim IS, Kim SY (2003). Structure and Mucous Histochemistry of the Intestinal Respiratory Tract of the Mud Loach, Misgurnus anguillicaudatus (Cantor), J App Ichthy, 19, 215–219. Raji AR, Norouzi E (2010). Histological and Histochemical Study on the Alimentary Canal in Walking Catfish (Claris Batrachus) and Piranha (Serrasalmus nattereri). Iranian J Res, Shiraz Univ, 11(3), 255-261 Selver MM, Aydoğdu A, Çırak VY (2010). Kocadere Deresi (Bursa)’ndeki Tahta Balıkları (Blicca bjoerkna L. 1758)’nın Helmint Parazitleri. T Parazitol Derg, 34 (2), 118–121.
Comparative Histochemical Studies on the Stomach and Intestine Tissues of the White Bream (Blicca bjoerkna (L., 1758)), in Lake Uluabat
Öz
The present investigation aims to illustrate the histological and
histochemical differences of the stomach and intestine of the white bream (Blicca bjoerkna (L., 1758)). The
digestive system of Blicca bjoerkna
is morphologically divided into four portions: stomach, anterior intestine
middle intestine and posterior intestine portions. Species were provided from adult roach fish located in Uluabat lake. Stomach and intestine
sampling was performed with abdominal dissection after application of
anesthesia clove oil. The
morphological and histochemical structures of all samples were studied with a
light microscope. PAS, PAS/AB pH 2.5, AB pH 2.5, AF, AF/AB pH 2.5 histochemical stainings
were performed for determining mucosubstance features, density and distribution.
Anahtar Kelimeler
Kaynakça
- Banan-Khojasteh SM, Sheikhzadeh F, Mohammadnejad D, Azami A (2009). Histological, Histochemical and Ultrastructural Study of the Intestine of Rainbow Trout (Oncorhynchus mykiss), World Appl Sci J, 6(11), 1525-1531. Bosi G, Shinn AP, Giari L, Simoni E, Pironi F, Dezfuli BS (2005). Changes in the Neuromodulators of the Diffuse Endocrine System of the Alimentary Canal of Farmed Rainbow Trout. Oncorhynchus mykiss (Walbaum), Naturally Infected with Eubothrium crassum (Cestoda). J Fish Dis, 28, 703-711. Çınar Ş, Küçükkara R, Balık İ, Çubuk H, Ceylan M, Erol K G, Yeğen V, Bulut C (2013). Uluabat (Apolyont) Gölü’ndeki Balık Faunasının Tespiti, Tür Kompozisyonu ve Ticari Avcılığın Türlere Göre Dağılımı. J Fish Sci, 7(4), 309-316. Çınar Ş, Küçükkara R, Ceylan M, Çubuk H, Erol K G, Akçimen U, Savaşer S (2008). Uluabat Gölü’ndeki Kızılkanat (Scardinius erythrophthalmus L.,1758) Populasyonu’nun Büyüme Parametrelerinin Araştırılması. Ege Üniv Su Ürün Derg, 25, (4), 289–293. Desantis S, Acone F, Zizza S, Deflorio M, Fernandez JLP, Sarasquete C, De Metrio G (2009). Glycohistochemical Study of the Toadfish Halobatrachus didactylus (Scheider, 1801) Stomach. Sci Mar, 73(3), 515- 525. Diaz AO, Garcia AM, Devincenti CV, Goldemberg AL (2003). Morphological and Histochemical Characterization of the Mucosa of the Digestive Tract in Engraulis anchoita. Anat Histol Embryol, 32, 341–346. Diaz AO, Garcia AM, Figueroa DE, Goldemberg AL (2007). The Mucosa of Digestive Tract in Micropogonias furnieri: A Light and Electron Microscope Approach, Anat Histol Embryol, 37, 251–256. Gomari (1952). Gomari’s Aldehyde Fuchsin stain. In: Cellular Pathology Technique (C F A Culling, R T Allison and W T Barr, eds). Butterworths, London. Khojasteh SMB, Ghodratiniya S (2013). Gastric and Intestinal Morphohistology of Epinephelus Coioides (Osteichthyes, Serranidae). Int J Aquac Sci, 4 (2), 83-90. Leknes IL (2010). Histochemical Study on the Intestine Goblet Cells in Cichlid and Poecilid Secies (Teleostei). Tissue and Cell, 42, 61-64. Leknes IL (2013). Goblet Cell Types in Intestine of Tiger Barb nd Black Tetra (Cyprinidae, Characidae: Teleostei). Anat Histol Embryol, doi: 10.1111/ahe.12083. Lev R, Spicer SS (1964). Specific staining of sulphate groups with alcian blue at low pH. J Histochem Cytochem, 12, 309 McManus JFA (1948). Histological and Histochemical Uses of Periodic Acid. Stain Technol, 23, 99–108. Mowry RW (1956). Alcian Blue Techniques for the Histochemical Study of Acidic Carbohydrates. J Histochem Cytochem, 4, 407–408. Neuhaus H, Van Der Marel M, Caspari N, Meyeri W, Enss ML, Steinhagen D (2007). Biochemical and Histochemical Study on the Intestinal Mucosa of the Common Carp Cyrprinus carpio L., with Special Consideration of Mucin Glycoproteins. J Fish Biol, 70, 1523-1534. Park JY, Kim IS, Kim SY (2003). Structure and Mucous Histochemistry of the Intestinal Respiratory Tract of the Mud Loach, Misgurnus anguillicaudatus (Cantor), J App Ichthy, 19, 215–219. Raji AR, Norouzi E (2010). Histological and Histochemical Study on the Alimentary Canal in Walking Catfish (Claris Batrachus) and Piranha (Serrasalmus nattereri). Iranian J Res, Shiraz Univ, 11(3), 255-261 Selver MM, Aydoğdu A, Çırak VY (2010). Kocadere Deresi (Bursa)’ndeki Tahta Balıkları (Blicca bjoerkna L. 1758)’nın Helmint Parazitleri. T Parazitol Derg, 34 (2), 118–121.
Toplam 1 adet kaynakça vardır.
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Konular | Veteriner Cerrahi |
| Bölüm | Makaleler |
| Yazarlar | |
| Yayımlanma Tarihi | 20 Aralık 2014 |
| Gönderilme Tarihi | 21 Mayıs 2014 |
| Kabul Tarihi | 23 Haziran 2014 |
| Yayımlandığı Sayı | Yıl 2014 Cilt: 25 Sayı: 3 |
Kaynak Göster
Kabul edilen makaleler Creative Commons Atıf-Ticari Olmayan Lisansla Paylaş 4.0 uluslararası lisansı ile lisanslanmıştır.