Glifosat Bazlı Herbisitin Erkek Nil Tilapiyası (Oreochromis niloticus) Üreme Dokuları ve Sperm Hücreleri Üzerine Etkileri
Öz
Glifosat bazlı formülasyonlar, dünyada en yaygın kullanılan herbisit tarım ilaçlarındandır. Bu çalışmada, su kaynaklı glifosatın erkek Nil Tilapyası (Oreochromis niloticus) testis, sperm kanalı, testiküler ve sağım yolu ile elde edilmiş spermatozoa örnekleri üzerine etkileri belirlenmiştir. Bu amaçla, balıklar 14 gün boyunca 0, 5, 10, 20, 30 ve 40 mg/L glifosat içeren deneme akvaryumlarında tutulmuşlardır. >10 mg/L glifosatın, sağım yoluyla ve testislerden alınmış olan spermatozoa örneklerinin motilite, canlılık süresi ve vitalite değerlerini azaltıcı etkisi gözlemlenmiştir. 40 mg/L glifosat grubunda hem testiküler hem de sağım yoluyla elde edilmiş spermatozoa örneklerinde en düşük vitalite değerleri saptanmıştır. >10 mg/L üzerindeki glifosat maruziyetinin, spermatozoa hücrelerinin oksidatif dengesinde değişimlere yol açtığı belirlenmiştir. Ayrıca, testis ve sperm kanalı dokularında glifosat etkisi ile oluşan lipid peroksidasyon seviyelerindeki azalmaların, kontrol grubuna alınan testis örneği haricinde istatistiki açıdan önemli farklar göstermediği saptanmıştır. Katalaz aktivitesi ise kontrol grubuna kıyasla >5mg/L glifosat gruplarında özellikle testis dokularında artış göstermektedir. Sperm kanalı dokusundaki oksidatif cevap, testis dokusundan farklı olduğu belirlenmiştir. Sonuç olarak, bu çalışma ile glifosat bazlı yaygın olarak kullanılan ticari bir herbisitin, erkek Nil Tilapyası balığı üreme sistemi üzerine etkileri gösterilmiştir. Ortamda bulunan özellikle 5 mg/L’den fazla olan glifosat konsantrasyonun spermatozoa parametrelerine olumsuz yansıdığı saptanmıştır. Bununla birlikte daha yüksek konsantrasyonların bu herbisitin üreme sistemi dokularındaki oksidatif stres koşullarını etkileyebildiği gösterilmiştir.
Anahtar Kelimeler
Glifosat Oreochromis niloticus testis sperm kanalı spermatozoa
Destekleyen Kurum
Bu çalışma Çanakkale Onsekiz Mart Üniversitesi Bilimsel Araştırmalar Proje Birimi
Proje Numarası
FBA-2019-2867
Teşekkür
Bu çalışma Çanakkale Onsekiz Mart Üniversitesi Bilimsel Araştırmalar Proje Birimi tarafından des-teklenmiştir. Proje No: FBA-2019-2867.
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Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Konular | Ziraat, Veterinerlik ve Gıda Bilimleri |
| Bölüm | Araştırma Makaleleri |
| Yazarlar | |
| Proje Numarası | FBA-2019-2867 |
| Yayımlanma Tarihi | 18 Ekim 2022 |
| Gönderilme Tarihi | 25 Mayıs 2022 |
| Yayımlandığı Sayı | Yıl 2022 Cilt: 9 Sayı: 4 |
