Öz
Amaç: Bu çalışmada prokalsitonin test performansının ayrı kit ve cihazlarda karşılaştırması incelenmiştir. Prokalsitonin (PCT), Kalsitoninin 116 amino asitten oluşan prohormonudur. Serumda PCT seviyelerindeki yükselmeler ciddi sepsis ve viral enfeksiyonlar ile ilişkilidir. Serumdaki PCT seviyesi genellikle bakteriyel enfeksiyonu olan hastalarda görülür, bu nedenle bakteriyel ve viral bulaşıcı hastalıkları ayırt etmek için güvenli bir biyobelirteç olarak kullanılmaktadır. PCT' nin kantitatif ölçümünün sepsisin prognozunu değerlendirmeye ve antibiyotik başlatma ve sonlandırmaya rehberlik etmeye yardımcı olabileceği bilinmektedir. Serum PCT' nin kantitatif ölçümü için yöntemler esas olarak floresan, kemilüminesan ve elektrokemilüminesan immünoanaliz gibi yöntemleri içermektedir.
Gereç ve Yöntemler: Hastaneden alınan 40 hasta örneği Prokalsitonin ölçümü için toplandı ve etik kurul onayı alındı.
Prokalsitonin ölçümleri biyokimya laboratuvarında ARCHITECT ci4000 system (CMIA, Abbott Diagnostic) ile Autolumo A2000 Plus (CLIA, Autobio Diagnostic) sistem cihazlarda ardışık olarak yapıldı. Yöntem karşılaştırma çalışmaları Clinical Laboratory Standards Institue tarafından yayımlanan National Committee for Clinical Laboratory Standards (NCCLS)’in EP15-A2 protokolüne göre gerçekleştirildi.
Bulgular: Çalışmamızda hasta örneklerinin prokalsitonin konsantrasyonları 0,01-53,1 ng/ml arasındaydı. Hasta sonuçlarının her iki cihaz için ortalama ve standart sapma değerleri X=7,71±1,22 ve Y=8,4±1,32 idi. Her iki yöntem ile ölçülen hasta sonuçları karşılaştırıldığında istatistiksel olarak anlamlı bir fark elde edilmedi ( p>0,05).
Sonuç: Prokalsitonin için her iki cihazda da ölçüm sonuçlarının korelasyonunu inceledik ve sonuçların birbiriyle korale olduğunu tespit ettik.
Anahtar Kelimeler
prokalsitonin kemilüminesan immunassay CLIA analitik performans
Destekleyen Kurum
Bulunmamaktadır
Proje Numarası
-
Teşekkür
Bu çalışmaya destek olan Istanbul Üniversitesi Medicine Hastanesi Tıbbi Laboratuvar çalışanlarına teşekkür ederiz.
Kaynakça
- Becker KL, Snider R., Nylen, ES. Procalcitonin in sepsis and systemic inflammation: a harmful biomarker and a therapeutic target. Br J Pharmacol, 2010; 159(2), 253-264. https://doi.org/10.1111/j.1476-5381.2009.00433.x
- Meisner M. Procalcitonin - Biochemistry and Clinical Diagnosis. 1st ed. Bremen: UNI-MED; 2010. ISBN 978- 3-8374-1241-3
- Mahmoud, S. A., El-Rayyes, A., Ahmed, S. S., & Attia, M. S. (2023). Procalcitonin assessment by using optical sensor terbium hydrochlorothiazide complex for early diagnosis of lung cancer. Microchemical Journal, 193. https://doi.org/ARTN 10905110.1016/j.microc.2023.109051
- Sincan, S., Sincan, G., Karakose, M. N., Aksoz, Z., & Erdem, F. (2023). The Importance of Serum Procalcitonin and C-reactive Protein Levels in Patients with Lymphoma. Meandros Medical and Dental Journal, 24(2), 168-174. https://doi.org/10.4274/meandros.galenos.2023.74317
- Mancini, N. (2015). Sepsis : diagnostic methods and protocols. Humana Press : Springer.
- Galli, F., Bindo, F., Motos, A., Fernandez-Barat, L., Barbeta, E., Gabarrus, A., Ceccato, A., Bermejo-Martin, J. F., Ferrer, R., Riera, J., Penuelas, O., Lorente, J. A., de Gonzalo-Calvo, D., Menendez, R., Gonzalez, J., Misuraca, S., Palomeque, A., Amaya-Villar, R., Anon, J. M., . . . Inves, C. P. (2023). Procalcitonin and C-reactive protein to rule out early bacterial coinfection in COVID-19 critically ill patients. Intensive Care Medicine. https://doi.org/10.1007/s00134-023-07161-1
- Dipalo, M., Gnocchi, C., Avanzini, P., Musa, R., Di Pietro, M., & Aloe, R. (2019). Comparison of Procalcitonin Assays on KRYPTOR and LIAISON((R)) XL Analyzers. Diagnostics (Basel), 9(3). https://doi.org/10.3390/diagnostics9030094
- Eidizadeh, A., Asif, A. R., von Ahsen, N., Binder, L., & Schnelle, M. (2019). Differences in procalcitonin measurements between three BRAHMS-partnered immunoassays (Liaison, Elecsys and Architect). Clin Chem Lab Med, 57(9), e207-e210. https://doi.org/10.1515/cclm-2018-0916
- Eidizadeh, A., Wiederhold, M., Schnelle, M., & Binder, L. (2022). Comparison of a novel automated DiaSys procalcitonin immunoassay with four different BRAHMS-partnered immunoassays. Pract Lab Med, 30, e00274. https://doi.org/10.1016/j.plabm.2022.e00274
- Liao, M. J., Zheng, J., Xu, Y., Qiu, Y. L., Xia, C., Zhong, Z. H., Liu, L. H., Liu, H. R., Liu, R. S., & Liang, S. Y. (2021). Development of magnetic particle-based chemiluminescence immunoassay for measurement of human procalcitonin in serum. Journal of Immunological Methods, 488. https://doi.org/ARTN 11291310.1016/j.jim.2020.112913
- Essmann, L., Wirz, Y., Gregoriano, C., & Schuetz, P. (2023). One biomarker does not fit all: tailoring anti-infective therapy through utilization of procalcitonin and other specific biomarkers. Expert Review of Molecular Diagnostics. https://doi.org/10.1080/14737159.2023.2242782
- Liu, Y., Chen, F., Bao, L., & Hai, W. (2022). Construction of a non-enzymatic electrochemical sensor based on graphitic carbon nitride nanosheets for sensitive detection of procalcitonin. RSC Adv, 12(35), 22518-22525. https://doi.org/10.1039/d2ra03650a
- Dipalo M, Guidoa L, Miccab G, Pittalisc S, Locatellid M, Mottad A, et al. Multicenter comparison of automated procalcitonin immunoassays. Practical Lab Med 2015;2:22-8.
- Farzami and Aliasgharpour Journal of Diabetes & Metabolic Disorders (2017) Chemiluminescence systems; do all lead to same results in prolactin analysis? 16:24 DOI 10.1186/s40200-017-0305-7
- Fortunato, A. A new sensitive automated assay for procalcitonin detection: LIAISONs BRAHMS PCTs II GEN. Pract. Lab. Med. 2016, 6, 1–7. [CrossRef] [PubMed]
- B.R.A.H.M.S PCT-Q Instruction manual. http://www. procalcitonin.com/pct-assays/pct-q-rapid.html (Accessed 5 January 2023)
- PCT CLIA Microparticles AutoLumo A2000 Plus Manual textbook. https://en.autobio.com.cn/Product/productDetail/fid/63/cid/1/id/143.html Accessed 5 January 2023
- Lippi, G.; Cervellin, G.F. Procalcitonin for diagnosing and monitoring bacterial infections: For or against? Clin. Chem. Lab. Med. 2018, 56, 1193–1195. [CrossRef] [PubMed]
Öz
Aim: In this study, the comparison of procalcitonin test performance in different kits and devices was examined. Procalcitonin (PCT) is the prohormone of Calcitonin, consisting of 116 amino acids. Elevations in PCT levels in serum are associated with severe sepsis and viral infections. PCT level in serum is often observed in patients with bacterial infection, so it is used as a safe biomarker to distinguish bacterial and viral infectious diseases. It is known that quantitative measurement of PCT can help assess the prognosis of sepsis and guide antibiotic initiation and discontinuation. Methods for quantitative measurement of serum PCT mainly include methods such as fluorescent, chemiluminescent and electrochemiluminescent immunoassay.
Material and Methods: 40 patient samples collected from the patients were collected for procalcitonin measurement and ethics committee approval was obtained.
Procalcitonin measurements were made consecutively in the biochemistry laboratory on ARCHITECT ci4000 system (CMIA, Abbott Diagnostic) and Autolumo A2000 Plus (CLIA, Autobio Diagnostic) system devices. Method comparison studies were carried out according to the EP15-A2 protocol of the National Committee for Clinical Laboratory Standards (NCCLS) published by the Clinical Laboratory Standards Institute.
Findings: In our study, procalcitonin concentrations of patient samples were between 0.01-53.1 ng/ml. The mean and standard deviation values of patient results for both devices were X=7.71±1.22 and Y=8.4±1.32. When patient results measured by both methods were compared, no statistically significant difference was obtained (p>0.05).
Results: We examined the correlation of measurement results for procalcitonin in both devices and found that the results were correlated with each other.
Anahtar Kelimeler
procalcitonin chemiluminescent immunoassay CLIA analytical performance
Proje Numarası
-
Kaynakça
- Becker KL, Snider R., Nylen, ES. Procalcitonin in sepsis and systemic inflammation: a harmful biomarker and a therapeutic target. Br J Pharmacol, 2010; 159(2), 253-264. https://doi.org/10.1111/j.1476-5381.2009.00433.x
- Meisner M. Procalcitonin - Biochemistry and Clinical Diagnosis. 1st ed. Bremen: UNI-MED; 2010. ISBN 978- 3-8374-1241-3
- Mahmoud, S. A., El-Rayyes, A., Ahmed, S. S., & Attia, M. S. (2023). Procalcitonin assessment by using optical sensor terbium hydrochlorothiazide complex for early diagnosis of lung cancer. Microchemical Journal, 193. https://doi.org/ARTN 10905110.1016/j.microc.2023.109051
- Sincan, S., Sincan, G., Karakose, M. N., Aksoz, Z., & Erdem, F. (2023). The Importance of Serum Procalcitonin and C-reactive Protein Levels in Patients with Lymphoma. Meandros Medical and Dental Journal, 24(2), 168-174. https://doi.org/10.4274/meandros.galenos.2023.74317
- Mancini, N. (2015). Sepsis : diagnostic methods and protocols. Humana Press : Springer.
- Galli, F., Bindo, F., Motos, A., Fernandez-Barat, L., Barbeta, E., Gabarrus, A., Ceccato, A., Bermejo-Martin, J. F., Ferrer, R., Riera, J., Penuelas, O., Lorente, J. A., de Gonzalo-Calvo, D., Menendez, R., Gonzalez, J., Misuraca, S., Palomeque, A., Amaya-Villar, R., Anon, J. M., . . . Inves, C. P. (2023). Procalcitonin and C-reactive protein to rule out early bacterial coinfection in COVID-19 critically ill patients. Intensive Care Medicine. https://doi.org/10.1007/s00134-023-07161-1
- Dipalo, M., Gnocchi, C., Avanzini, P., Musa, R., Di Pietro, M., & Aloe, R. (2019). Comparison of Procalcitonin Assays on KRYPTOR and LIAISON((R)) XL Analyzers. Diagnostics (Basel), 9(3). https://doi.org/10.3390/diagnostics9030094
- Eidizadeh, A., Asif, A. R., von Ahsen, N., Binder, L., & Schnelle, M. (2019). Differences in procalcitonin measurements between three BRAHMS-partnered immunoassays (Liaison, Elecsys and Architect). Clin Chem Lab Med, 57(9), e207-e210. https://doi.org/10.1515/cclm-2018-0916
- Eidizadeh, A., Wiederhold, M., Schnelle, M., & Binder, L. (2022). Comparison of a novel automated DiaSys procalcitonin immunoassay with four different BRAHMS-partnered immunoassays. Pract Lab Med, 30, e00274. https://doi.org/10.1016/j.plabm.2022.e00274
- Liao, M. J., Zheng, J., Xu, Y., Qiu, Y. L., Xia, C., Zhong, Z. H., Liu, L. H., Liu, H. R., Liu, R. S., & Liang, S. Y. (2021). Development of magnetic particle-based chemiluminescence immunoassay for measurement of human procalcitonin in serum. Journal of Immunological Methods, 488. https://doi.org/ARTN 11291310.1016/j.jim.2020.112913
- Essmann, L., Wirz, Y., Gregoriano, C., & Schuetz, P. (2023). One biomarker does not fit all: tailoring anti-infective therapy through utilization of procalcitonin and other specific biomarkers. Expert Review of Molecular Diagnostics. https://doi.org/10.1080/14737159.2023.2242782
- Liu, Y., Chen, F., Bao, L., & Hai, W. (2022). Construction of a non-enzymatic electrochemical sensor based on graphitic carbon nitride nanosheets for sensitive detection of procalcitonin. RSC Adv, 12(35), 22518-22525. https://doi.org/10.1039/d2ra03650a
- Dipalo M, Guidoa L, Miccab G, Pittalisc S, Locatellid M, Mottad A, et al. Multicenter comparison of automated procalcitonin immunoassays. Practical Lab Med 2015;2:22-8.
- Farzami and Aliasgharpour Journal of Diabetes & Metabolic Disorders (2017) Chemiluminescence systems; do all lead to same results in prolactin analysis? 16:24 DOI 10.1186/s40200-017-0305-7
- Fortunato, A. A new sensitive automated assay for procalcitonin detection: LIAISONs BRAHMS PCTs II GEN. Pract. Lab. Med. 2016, 6, 1–7. [CrossRef] [PubMed]
- B.R.A.H.M.S PCT-Q Instruction manual. http://www. procalcitonin.com/pct-assays/pct-q-rapid.html (Accessed 5 January 2023)
- PCT CLIA Microparticles AutoLumo A2000 Plus Manual textbook. https://en.autobio.com.cn/Product/productDetail/fid/63/cid/1/id/143.html Accessed 5 January 2023
- Lippi, G.; Cervellin, G.F. Procalcitonin for diagnosing and monitoring bacterial infections: For or against? Clin. Chem. Lab. Med. 2018, 56, 1193–1195. [CrossRef] [PubMed]
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Konular | İmmünoloji (Diğer), Klinik Mikrobiyoloji |
| Bölüm | Özgün Makale |
| Yazarlar | |
| Proje Numarası | - |
| Yayımlanma Tarihi | 30 Aralık 2023 |
| Yayımlandığı Sayı | Yıl 2023 Cilt: 14 Sayı: 4 |
Kaynak Göster
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