Öz
Özet
Amaç: PRP nin nazal mukozada yara iyileşmesi üzerine olan etkilerini incelemek
Gereç ve yöntem: Çalışmamızda ağırlıkları 2.8-3.5 kg (ortalama 3.2 kg) ağırlığında 32 adet erkek Yeni Zelanda beyaz tavşanı kullanıldı. Sağ nazal kavitelerine girilerek interdental fırça yardımı ile (brushing technique) septum, konka ve lateral nazal duvarlarında mukozal hasar oluşturuldu. Hayvanlar birinci hafta ve üçüncü hafta olmak üzere iki gruba ve bu iki grup da kontrol ve PRP olmak üzere iki alt gruba ayrıldı.
Bulgular: Histopatolojik skorlama sonuçlarına göre birinci hafta grubunda inflamasyonun şiddeti parametresi açısından istatistiksel olarak anlamlı fark saptandı (p=0,009).Diğer parametreler açısından birinci hafta ve üçüncü hafta gruplarında istatistiksel olarak anlamlı fark saptanmadı.
Sonuçlar: Sonuçlarımız PRP nin yara iyileşmesinin erken aşamalarında inflamasyonu azalttığını göstermektedir. PRP nin nazal mukozal yara iyileşmesi üzerindeki etkilerinin anlaşılabilmesi için ileri çalışmalara ihtiyaç olduğu düşünülmektedir.
Anahtar Kelimeler
Kaynakça
- 1. Arnoczky SP, Sheibani-Rad S. The basic science of platelet-rich plasma (PRP): what clinicians need to know. Sports Med Arthrosc. 2013;21(4):180-5.
- 2. Fennis JP, Stoelinga PJ, Jansen JA. Mandibular reconstruction: a histological and histomorphometric study on the use of autogenous scaffolds, particulate cortico-cancellous bone grafts and platelet rich plasma in goats. Int J Oral Maxillofac Surg. 2004;33(1):48-55.
- 3. Hom DB. Growth factors and wound healing in otolaryngology. Otolaryngol Head Neck Surg. 1994;110(6):560-4.
- 4. Marx RE, Carlson ER, Eichstaedt RM, Schimmele SR, Strauss JE, Georgeff KR. Platelet-rich plasma: Growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1998;85(6):638-46.
- 5. Wroblewski AP, Mejia HA, Wright VJ. Application of platelet-rich plasma to enhance tissue repair. Operative Techniques in Orthopaedics. 2010;20(2):98-5.
- 6. Erkilet E, Koyuncu M, Atmaca S, Yarim M. Platelet-rich plasma improves healing of tympanic membrane perforations: experimental study. J Laryngol Otol. 2009;123(5):482-7.
- 7. Khalmuratova R, Jeon SY, Kim DW, Kim JP, Ahn SK, Park JJ, et al. Wound healing of nasal mucosa in a rat. Am J Rhinol Allergy. 2009;23(6):e33-7.
- 8. Salaheldin AH, Hussein A. Effect of platelet-rich plasma on nasal mucociliary clearance after submucous diathermy of inferior turbinate. Egyptian Journal of Ear, Nose, Throat and Allied Sciences.2012;13(2):71-5.
- 9. Nolte D, Zeintl H, Steinbauer M, Pickelmann S, Messmer K. Functional capillary density: an indicator of tissue perfusion? Int J Microcirc Clin Exp. 1995;15(5):244-9
- 10. Lindeboom JA, Mathura KR, Aartman IH, Kroon FH, Milstein DM, Ince C. Influence of the application of platelet-enriched plasma in oral mucosal wound healing. Clin Oral Implants Res. 2007;18(1):133-9.
- 11. Kakudo N, Kushida S, Minakata T, Suzuki K, Kusumoto K. Platelet-rich plasma promotes epithelialization and angiogenesis in a splitthickness skin graft donor site. Med Mol Morphol. 2011;44(4):233-6.
Öz
Abstract
Objective: Study
the effects of platelet-rich plasma (PRP) on wound healing
in nasal mucosa.
Materials and
Methods: Thirty two adult New Zealand white rabbit of
male sex were used for this study each weighing between 2800 and 3500
grams.Mucosal damage was created by interdental brush (brushing technique). The
animals were randomly distributed into two groups as first week group and third
week group. This two groups were subdivided into two subgroups as control and
PRP.
Results:
According to the histopathologic scoring results, in first week group
statistically significant difference was observed in term of inflammation
severity between PRP and control subgroups (p =0.009). No
statistically significant difference was observed between the other groups in
terms of mucosal edema severity, loss of cilia degree between ciliary
cells,fibrosis severity, goblet cell hyperplasia, inflammation severity, congestion
and squamous metaplasia.
Conclusion: Our results show that PRP reduces inflammation in early stages of
wound healing. We believe further more studies are needed to understand the effects
of PRP on nasal mucosal wound healing.
Anahtar Kelimeler
Kaynakça
- 1. Arnoczky SP, Sheibani-Rad S. The basic science of platelet-rich plasma (PRP): what clinicians need to know. Sports Med Arthrosc. 2013;21(4):180-5.
- 2. Fennis JP, Stoelinga PJ, Jansen JA. Mandibular reconstruction: a histological and histomorphometric study on the use of autogenous scaffolds, particulate cortico-cancellous bone grafts and platelet rich plasma in goats. Int J Oral Maxillofac Surg. 2004;33(1):48-55.
- 3. Hom DB. Growth factors and wound healing in otolaryngology. Otolaryngol Head Neck Surg. 1994;110(6):560-4.
- 4. Marx RE, Carlson ER, Eichstaedt RM, Schimmele SR, Strauss JE, Georgeff KR. Platelet-rich plasma: Growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1998;85(6):638-46.
- 5. Wroblewski AP, Mejia HA, Wright VJ. Application of platelet-rich plasma to enhance tissue repair. Operative Techniques in Orthopaedics. 2010;20(2):98-5.
- 6. Erkilet E, Koyuncu M, Atmaca S, Yarim M. Platelet-rich plasma improves healing of tympanic membrane perforations: experimental study. J Laryngol Otol. 2009;123(5):482-7.
- 7. Khalmuratova R, Jeon SY, Kim DW, Kim JP, Ahn SK, Park JJ, et al. Wound healing of nasal mucosa in a rat. Am J Rhinol Allergy. 2009;23(6):e33-7.
- 8. Salaheldin AH, Hussein A. Effect of platelet-rich plasma on nasal mucociliary clearance after submucous diathermy of inferior turbinate. Egyptian Journal of Ear, Nose, Throat and Allied Sciences.2012;13(2):71-5.
- 9. Nolte D, Zeintl H, Steinbauer M, Pickelmann S, Messmer K. Functional capillary density: an indicator of tissue perfusion? Int J Microcirc Clin Exp. 1995;15(5):244-9
- 10. Lindeboom JA, Mathura KR, Aartman IH, Kroon FH, Milstein DM, Ince C. Influence of the application of platelet-enriched plasma in oral mucosal wound healing. Clin Oral Implants Res. 2007;18(1):133-9.
- 11. Kakudo N, Kushida S, Minakata T, Suzuki K, Kusumoto K. Platelet-rich plasma promotes epithelialization and angiogenesis in a splitthickness skin graft donor site. Med Mol Morphol. 2011;44(4):233-6.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Cerrahi |
| Bölüm | Araştırma Makaleleri |
| Yazarlar | |
| Yayımlanma Tarihi | 1 Aralık 2019 |
| Gönderilme Tarihi | 13 Kasım 2018 |
| Kabul Tarihi | 13 Aralık 2018 |
| Yayımlandığı Sayı | Yıl 2019 Cilt: 26 Sayı: 4 |
Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi/Medical Journal of Süleyman Demirel University is licensed under Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International.