EFFECT OF MELATONIN ON SPINAL CORD INJURY INDUCED LIPID PEROXIDATION IN RATS: PROGNOSTIC VALUE OF MALONDIALDEHYDE
Öz
Melatonin is a potent antioxidant agent produced by the pineal gland. In this study we aimed to investigate the neuro-protective effect of melatonin on ischemic spinal cord in the rats. Aortic clamp was used for the occlusion of the abdominal aorta for 30 minutes. Twenty four Winstar male rats were used for this study and rats were divided randomly into 4 groups: control group (sham group, n: 5), sham+melatonin treated group (receiving 10mg/kg melatonin intraperitoneally, n: 5), ischemia/reperfusion group (undergoing occlusion but receiving no pharmacologic intervention, n: 7), melatonin + ischemia/reperfusion group (undergoing occlusion and receiving 10mg/kg melatonin intraperitoneally 10 minutes after ischemia, n: 7). Spinal cord samples were taken for the malondialdehyde (MDA) analyses. In the aort occlusion group, the MDA levels, indicating the extend of lipid peroxidation, were significantly higher than the other groups (p<0.05). Also in the aort occlusion + melatonin group the levels of MDA was significantly reduced compared to the aort occlusion group (p<0.05). In this study, we showed that injection of melatonin reduces the effects of secondary injury and facilitate the recovery of the damaged spinal cord.
Anahtar Kelimeler
Kaynakça
- Cadotte DW, Singh A, Fehlings MG. The timing of surgical decompression for spinal cord injury. Medicine Reports 2010;2:67-4.
- Fujimoto T, Nakamura T, Ikeda T, Tagaki K. Potent protective effects of melatonin on experimental spinal cord injury. Spine 2000;25(7):769-775.
- Taoka Y, Okajima K. Spinal cord injury in the rat. Prog Neurobiol 1998;55:1-18.
- Anderson DK, Hall ED. Pathophysiology of spinal cord trauma. Ann Emerg Med 1993;22:987-92.
- Fantone JC, Ward PA. Role of oxygen- derived free radicals and metabolites in leukocyte-dependent inflammatory reactions. Am J Pathol 1982;107:395-418.
- Cassone VM, Chesworth MJ, Armstrong SM. Entrainment of rat circadian rhythms by daily injection of melatonin depends upon the hypothalamic suprachiasmatic nuclei. Physiol Behav 1986;36:1111-21.
- Reiter RJ, Tan DX, Poeggeler B,Menedez- Pelaez A, Chen LD, Saarela S. Melatonin as a free radical scavenger:Implications for aging and age-related diseases. Ann N Y Acad Sci 1994;719:1-14.
- De L Lastra CA, Cabeza J, Motilva V, Martin MJ. Melatonin protects against gastric ischemia-reperfusion injury in rats. J Pineal Res 1997;23;47-52.
- Draper H.H., Hadley M. Malondialdehyde determination as index of lipid peroxidation. Methods Enzymol. 1990;186: 421-431.
- Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin 1951;193:265-275.
- Bracken MB, Shepard MJ, Holford TR, Leo-Summers L, Aldrich EF, Fazl M, Fehlings MG, Herr DL, Hitchon FH et al. Methylprednisolone or tirilazad mesylate administration after spinal cord injury:1 year follow up:Results of the third national acute spinal cord injury randomized controlled trial. J Neurosurg 1998;89:699-706.
- Barut S, Canbolat A, Bilge T, Aydin Y, Cokneseli B, Kaya U. Lipid peroxidation in experimental spinal cord injury. Time-level relationship. Neurosurg Rev 1993;16: 53-59.
- Tuzgen S, Kaynar MY, Guner A, Gumustas K, Belce A, Etus V, Ozyurt E. The effect
- peroxidation after experimental spinal cord injury. Spinal Cord 1998;36:654-657.
- Erten SF, Kocak A, Ozdemir I,Aydemir S, Colak A, Reeder BS. Protective effect of melatonin on experimental spinal cord ischemia. Spinal Cord 2003;41:533-538.
- Oyar E.O, Korkmaz A, Kardes O, Omeroglu S. Aortic cross-clamping-induced spinal cord oxidative stress in rabbits: the role of a novel antioxidant adrenomedullin. J our of Surgical Research 2008;147:143-147. 16.
- Pharmacological approaches to repair the injured 2006;23:318.
- Tan DX, Manchester LC, Reiter RJ, Qi WB, Karbownik M, Calvo JR. Significance of melatonin
- system:reactions and products. Biol Signals Recept 2000;9:137-159.
- Marshall KA, Reiter RJ, Poeggeler B, Aruoma OI, Halliwell B. Evaluation of the antioxidant activity of melatonin in vitro. Free Radio Biol Med 1996;21:307-315.
- Korkmaz A, Oyar EO, Kardes O, Omeroglu S. Effects of melatonin on ischemic spinal cord injury caused by aortic cross clamping in rabbits. Curr Neurovasc Res. 2008;5(1):46-51. cooling on lipid Baptiste DC, Fehlings MG. spinal cord. J Neurotrauma in antioxidative defence
RATLARDA LİPİD PEROKSİDASYONUNUN İNDÜKLEDİĞİ SPİNAL KORD HASARINDA MELATONİNİN ETKİSİ: MALONDİALDEHİTİN TANISAL ÖNEMİ
Öz
Melatonin, pineal bez tarafından üretilen güçlü bir antioksidan ajandır. Bu çalışmada biz, ratlarda spinal kord iskemisinde melatoninin nöroprotektif etkisini araştırmayı amaçladık. Abdominal aortun oklüzyonu için 30dk süre ile aortik klemp kullanıldı. Çalışmada 24 Winstar erkek rat kullanıldı ve ratlar randomize olarak 4 gruba ayrıldı: kontrol grubu (sham grup, n:5), sham + melatonin verilen grup (10mg/kg melatonin intraperitoneal olarak verildi, n:5), iskemi/reperfüzyon grubu (oklüzyon yapılan ancak herhangi bir farmakolojik ajan verilmeyen grup, n:5), melatonin + iskemi/reperfüzyon grubu (oklüzyon yapılan ve iskemiden 10 dk sonra intraperitoneal olarak 10mg/kg melatonin verilen grup). Spinal kord örnekleri malondialdehit (MDA) analizi için alındı. Aort oklüzyonu yapılan grupta, lipid peroksidasyonunun olduğunu gösteren MDA düzeyleri diğer gruplardan belirgin olarak daha yüksekti (p<0.05). Aort oklüzyonu+melatonin verilen grupta da aort oklüzyonu yapılan grup ile karşılaştırıldığında MDA düzeylerinin belirgin olarak azaldığı görüldü (p<0.05). Bu çalışmada biz, melatonin enjeksiyonunun sekonder hasarın etkilerini azalttığını ve hasarlı spinal kordun iyileşmesini kolaylaştırdığını gösterdik.
Anahtar Kelimeler
melatonin iskemi/reperfüzyon spinal kord oksidatif stress malondialdehit
Kaynakça
- Cadotte DW, Singh A, Fehlings MG. The timing of surgical decompression for spinal cord injury. Medicine Reports 2010;2:67-4.
- Fujimoto T, Nakamura T, Ikeda T, Tagaki K. Potent protective effects of melatonin on experimental spinal cord injury. Spine 2000;25(7):769-775.
- Taoka Y, Okajima K. Spinal cord injury in the rat. Prog Neurobiol 1998;55:1-18.
- Anderson DK, Hall ED. Pathophysiology of spinal cord trauma. Ann Emerg Med 1993;22:987-92.
- Fantone JC, Ward PA. Role of oxygen- derived free radicals and metabolites in leukocyte-dependent inflammatory reactions. Am J Pathol 1982;107:395-418.
- Cassone VM, Chesworth MJ, Armstrong SM. Entrainment of rat circadian rhythms by daily injection of melatonin depends upon the hypothalamic suprachiasmatic nuclei. Physiol Behav 1986;36:1111-21.
- Reiter RJ, Tan DX, Poeggeler B,Menedez- Pelaez A, Chen LD, Saarela S. Melatonin as a free radical scavenger:Implications for aging and age-related diseases. Ann N Y Acad Sci 1994;719:1-14.
- De L Lastra CA, Cabeza J, Motilva V, Martin MJ. Melatonin protects against gastric ischemia-reperfusion injury in rats. J Pineal Res 1997;23;47-52.
- Draper H.H., Hadley M. Malondialdehyde determination as index of lipid peroxidation. Methods Enzymol. 1990;186: 421-431.
- Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin 1951;193:265-275.
- Bracken MB, Shepard MJ, Holford TR, Leo-Summers L, Aldrich EF, Fazl M, Fehlings MG, Herr DL, Hitchon FH et al. Methylprednisolone or tirilazad mesylate administration after spinal cord injury:1 year follow up:Results of the third national acute spinal cord injury randomized controlled trial. J Neurosurg 1998;89:699-706.
- Barut S, Canbolat A, Bilge T, Aydin Y, Cokneseli B, Kaya U. Lipid peroxidation in experimental spinal cord injury. Time-level relationship. Neurosurg Rev 1993;16: 53-59.
- Tuzgen S, Kaynar MY, Guner A, Gumustas K, Belce A, Etus V, Ozyurt E. The effect
- peroxidation after experimental spinal cord injury. Spinal Cord 1998;36:654-657.
- Erten SF, Kocak A, Ozdemir I,Aydemir S, Colak A, Reeder BS. Protective effect of melatonin on experimental spinal cord ischemia. Spinal Cord 2003;41:533-538.
- Oyar E.O, Korkmaz A, Kardes O, Omeroglu S. Aortic cross-clamping-induced spinal cord oxidative stress in rabbits: the role of a novel antioxidant adrenomedullin. J our of Surgical Research 2008;147:143-147. 16.
- Pharmacological approaches to repair the injured 2006;23:318.
- Tan DX, Manchester LC, Reiter RJ, Qi WB, Karbownik M, Calvo JR. Significance of melatonin
- system:reactions and products. Biol Signals Recept 2000;9:137-159.
- Marshall KA, Reiter RJ, Poeggeler B, Aruoma OI, Halliwell B. Evaluation of the antioxidant activity of melatonin in vitro. Free Radio Biol Med 1996;21:307-315.
- Korkmaz A, Oyar EO, Kardes O, Omeroglu S. Effects of melatonin on ischemic spinal cord injury caused by aortic cross clamping in rabbits. Curr Neurovasc Res. 2008;5(1):46-51. cooling on lipid Baptiste DC, Fehlings MG. spinal cord. J Neurotrauma in antioxidative defence
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Bölüm | Araştırma Makaleleri |
| Yazarlar | |
| Yayımlanma Tarihi | 17 Kasım 2011 |
| Gönderilme Tarihi | 13 Eylül 2011 |
| Yayımlandığı Sayı | Yıl 2011 Cilt: 2 Sayı: 2 |
SDÜ Sağlık Bilimleri Dergisi, makalenin gönderilmesi ve yayınlanması dahil olmak üzere hiçbir aşamada herhangi bir ücret talep etmemektedir. Dergimiz, bilimsel araştırmaları okuyucuya ücretsiz sunmanın bilginin küresel paylaşımını artıracağı ilkesini benimseyerek, içeriğine anında açık erişim sağlamaktadır.