Araştırma Makalesi
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Yıl 2021, Cilt: 38 Sayı: 3, 272 - 276, 01.05.2021

Öz

Kaynakça

  • Chen, W., Hu, J., Zhang, Z., Chen, L., Xie, H., Dong, N., Chen Y., Zuguo Liu, Z., 2012. Localization and expression of Zonula Occludins-1 in the rabbit corneal epithelium following exposure to benzal konium chloride. PLoS ONE. 7(7): e40893.
  • Chiquoine, A.D.,1964. Observations on the early events of cadmium necrosis of the testis. AnatRec. 149:23–35.
  • Garg, V., Gard, S.P., 2011. Role of nitric oxide in male infertility. J Indian Acad. Forensic Med. 33(1). 65-68.
  • Gao, Y., Mruk, D., Chen, H., Lui, W.Y., Lee, W.M., Cheng, C.Y., 2017.Regulation of the blood-testis barrier by a local axis in the testis: role of laminin α2 in the basement membrane. FASEB J.31(2):584-597.
  • Hikim, A. P., Lue, Y., Yamamoto, C. M., Vera, Y., Rodriguez, S., Yen, P. H., Soeng, K., Wang, C., Swerdloff, R.S., 2003. Key apoptotic pathways for heat-induced programmed germ cell death in the testis. Endocrinology. 144, 3167–3175.
  • Jiang, X.H.,Bukhari, I., Zheng, W., Yin, S., Wang, Z., Cooke H.J., Shi, Q.H., 2014.Blood-testis barrier and spermatogenesis: lessons from genetically-modified mice.Asian J. Androl.16(4):572-80.
  • Kang Z., Qiao N., Liu G., Chen H., Tang Z., Ying Li Y., 2017. Copper-induced apoptosis and autophagy through oxidative stress-mediated mitochondrial dysfunction in male germ cells. Toxicol In Vitro. 61:104639.
  • Kumar, N., Singh, A.K., 2015. Trends of male factor infertility, an important cause of infertility: A review of literature. J. Hum.Reprod Sci. 8(4):191-6.
  • Lee, N.P., Cheng, C.Y., 2008. Nitric oxide and cyclic nucleotides: Their roles ınjunction dynamics and spermatogenesis. Adv. Exp. Med. Biol.636:172-85.
  • Lee, N.P.Y., Cheng, C.Y., 2003. Regulation of sertoli cell tight junction in the rat testis viathe nitric oxide synthase/soluble guanylate cyclase/cGMP/Protein Kinase G signaling pathway: an in vitro study. Endocrinology.144:3114-3129.
  • Nieschlag, E., Behre, H., Nieschlag, S., Van, Ahlen H. 2011. Male reproductive health and dysfunction. Internistische Praxis. Andrology. 51: 751.
  • Rumbold, A.R., Sevoyan, A., Oswald, T.K., Fernandez R.C., Davies, M.J., Moore, V.M., 2019. Impact of male factor infertility on offspring health and development. FertilSteril. 111(6):1047-1053.
  • Sapanidou, V., Taitzoglou, I., Tsakmakidis, I.,Kourtzelis, I., Fletouris, D., Theodoridis, A., Zervos, I., Tsantarliotou, M., 2015. Antioxidant effect of crocin on bovine sperm quality and in vitro fertilization. Theriogenology. 84(8):1273-82.
  • Skakkebaek, N.E., Rajpert De Meyts, E., Main, K.M., 2001. Testicular dysgenesis syndrome: an increasingly common developmental disorder with environmental aspects. Hum.Reprod 16: 972−8.
  • Sun,H.,Yang, B., Zhu, C., Liu, R., Wang, H., Li, W., 2013. Presence of metastasis-associated protein 1 in Sertoli cells is required for propercontact between sertoli cells and adjacent germ cells.Urology.81(1):66-73.
  • Vlajkovic, S., Cukuranovic, R., Bjelakovic, M.R., Stefanovic. V. 2012. Possible Therapeutic Use of Spermatogonial Stem Cells in the Treatment of Male Infertility: A Brief Overview. ScientificWorldJournal. 8.
  • Yan Cheng,C.,Dolores, D.M, 2012.The Blood-Testis Barrier and Its Implications for Male Contraception. PharmacolRev. 64(1): 16–64.
  • WHO.,2010. World Health organization laboratory manual for the examination and the processing of human semen. 5 th ed;pp161.

Ultrastructural examination of testicular tissues and evaluation of ZO-1 protein levels obtained from azoospermic cases

Yıl 2021, Cilt: 38 Sayı: 3, 272 - 276, 01.05.2021

Öz

Spermatogenesis is a process that involves cellular and biochemical processes. The purpose of this study is to understand the role of tight junctions in spermatogenesis by evaluating Zonula occludens-1 (ZO-1) protein levels of cells from the testicular sperm extraction (TESE) biopsies in patients with azoospermia
The study included 40 infertile men with azoospermia. They were divided into two groups, as spermatozoa positive (n=20) and spermatozoa negative (n=20). Testis tissues were examined morphologically, immunohistochemically and ultrastructurally by using transmission electron microscopy (TEM). The structure of seminiferous tubules were deteriorated, degenerated and hyalinized in spermatozoa negative group. ZO-1 expression level was normal in blood-testis barrier of spermatozoa positive group in comparing to lateral surfaces of sertoli cells spermatozoa negative group. The ultrastructural results were also supported with light microscopy findings. These findings suggest that decrease of the ZO-1 protein expression level in Sertoli cells might be one of the factors involved in the impairment of spermatogenesis in spermatozoa negative group. Therefore, we suggest that tight junctions may have crucial roles in spermatogenesis.

Kaynakça

  • Chen, W., Hu, J., Zhang, Z., Chen, L., Xie, H., Dong, N., Chen Y., Zuguo Liu, Z., 2012. Localization and expression of Zonula Occludins-1 in the rabbit corneal epithelium following exposure to benzal konium chloride. PLoS ONE. 7(7): e40893.
  • Chiquoine, A.D.,1964. Observations on the early events of cadmium necrosis of the testis. AnatRec. 149:23–35.
  • Garg, V., Gard, S.P., 2011. Role of nitric oxide in male infertility. J Indian Acad. Forensic Med. 33(1). 65-68.
  • Gao, Y., Mruk, D., Chen, H., Lui, W.Y., Lee, W.M., Cheng, C.Y., 2017.Regulation of the blood-testis barrier by a local axis in the testis: role of laminin α2 in the basement membrane. FASEB J.31(2):584-597.
  • Hikim, A. P., Lue, Y., Yamamoto, C. M., Vera, Y., Rodriguez, S., Yen, P. H., Soeng, K., Wang, C., Swerdloff, R.S., 2003. Key apoptotic pathways for heat-induced programmed germ cell death in the testis. Endocrinology. 144, 3167–3175.
  • Jiang, X.H.,Bukhari, I., Zheng, W., Yin, S., Wang, Z., Cooke H.J., Shi, Q.H., 2014.Blood-testis barrier and spermatogenesis: lessons from genetically-modified mice.Asian J. Androl.16(4):572-80.
  • Kang Z., Qiao N., Liu G., Chen H., Tang Z., Ying Li Y., 2017. Copper-induced apoptosis and autophagy through oxidative stress-mediated mitochondrial dysfunction in male germ cells. Toxicol In Vitro. 61:104639.
  • Kumar, N., Singh, A.K., 2015. Trends of male factor infertility, an important cause of infertility: A review of literature. J. Hum.Reprod Sci. 8(4):191-6.
  • Lee, N.P., Cheng, C.Y., 2008. Nitric oxide and cyclic nucleotides: Their roles ınjunction dynamics and spermatogenesis. Adv. Exp. Med. Biol.636:172-85.
  • Lee, N.P.Y., Cheng, C.Y., 2003. Regulation of sertoli cell tight junction in the rat testis viathe nitric oxide synthase/soluble guanylate cyclase/cGMP/Protein Kinase G signaling pathway: an in vitro study. Endocrinology.144:3114-3129.
  • Nieschlag, E., Behre, H., Nieschlag, S., Van, Ahlen H. 2011. Male reproductive health and dysfunction. Internistische Praxis. Andrology. 51: 751.
  • Rumbold, A.R., Sevoyan, A., Oswald, T.K., Fernandez R.C., Davies, M.J., Moore, V.M., 2019. Impact of male factor infertility on offspring health and development. FertilSteril. 111(6):1047-1053.
  • Sapanidou, V., Taitzoglou, I., Tsakmakidis, I.,Kourtzelis, I., Fletouris, D., Theodoridis, A., Zervos, I., Tsantarliotou, M., 2015. Antioxidant effect of crocin on bovine sperm quality and in vitro fertilization. Theriogenology. 84(8):1273-82.
  • Skakkebaek, N.E., Rajpert De Meyts, E., Main, K.M., 2001. Testicular dysgenesis syndrome: an increasingly common developmental disorder with environmental aspects. Hum.Reprod 16: 972−8.
  • Sun,H.,Yang, B., Zhu, C., Liu, R., Wang, H., Li, W., 2013. Presence of metastasis-associated protein 1 in Sertoli cells is required for propercontact between sertoli cells and adjacent germ cells.Urology.81(1):66-73.
  • Vlajkovic, S., Cukuranovic, R., Bjelakovic, M.R., Stefanovic. V. 2012. Possible Therapeutic Use of Spermatogonial Stem Cells in the Treatment of Male Infertility: A Brief Overview. ScientificWorldJournal. 8.
  • Yan Cheng,C.,Dolores, D.M, 2012.The Blood-Testis Barrier and Its Implications for Male Contraception. PharmacolRev. 64(1): 16–64.
  • WHO.,2010. World Health organization laboratory manual for the examination and the processing of human semen. 5 th ed;pp161.
Toplam 18 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Sağlık Kurumları Yönetimi
Bölüm Clinical Research
Yazarlar

Ayşe Altun 0000-0002-2765-5766

Yasemin Ersoy Canıllıoğlu 0000-0002-3839-6931

Evrim Unsal 0000-0002-3965-7347

Seda Karabulut 0000-0003-3302-5004

Canan Hurdag 0000-0003-3336-0830

Yayımlanma Tarihi 1 Mayıs 2021
Gönderilme Tarihi 20 Kasım 2020
Kabul Tarihi 7 Şubat 2021
Yayımlandığı Sayı Yıl 2021 Cilt: 38 Sayı: 3

Kaynak Göster

APA Altun, A., Ersoy Canıllıoğlu, Y., Unsal, E., Karabulut, S., vd. (2021). Ultrastructural examination of testicular tissues and evaluation of ZO-1 protein levels obtained from azoospermic cases. Journal of Experimental and Clinical Medicine, 38(3), 272-276.
AMA Altun A, Ersoy Canıllıoğlu Y, Unsal E, Karabulut S, Hurdag C. Ultrastructural examination of testicular tissues and evaluation of ZO-1 protein levels obtained from azoospermic cases. J. Exp. Clin. Med. Mayıs 2021;38(3):272-276.
Chicago Altun, Ayşe, Yasemin Ersoy Canıllıoğlu, Evrim Unsal, Seda Karabulut, ve Canan Hurdag. “Ultrastructural Examination of Testicular Tissues and Evaluation of ZO-1 Protein Levels Obtained from Azoospermic Cases”. Journal of Experimental and Clinical Medicine 38, sy. 3 (Mayıs 2021): 272-76.
EndNote Altun A, Ersoy Canıllıoğlu Y, Unsal E, Karabulut S, Hurdag C (01 Mayıs 2021) Ultrastructural examination of testicular tissues and evaluation of ZO-1 protein levels obtained from azoospermic cases. Journal of Experimental and Clinical Medicine 38 3 272–276.
IEEE A. Altun, Y. Ersoy Canıllıoğlu, E. Unsal, S. Karabulut, ve C. Hurdag, “Ultrastructural examination of testicular tissues and evaluation of ZO-1 protein levels obtained from azoospermic cases”, J. Exp. Clin. Med., c. 38, sy. 3, ss. 272–276, 2021.
ISNAD Altun, Ayşe vd. “Ultrastructural Examination of Testicular Tissues and Evaluation of ZO-1 Protein Levels Obtained from Azoospermic Cases”. Journal of Experimental and Clinical Medicine 38/3 (Mayıs 2021), 272-276.
JAMA Altun A, Ersoy Canıllıoğlu Y, Unsal E, Karabulut S, Hurdag C. Ultrastructural examination of testicular tissues and evaluation of ZO-1 protein levels obtained from azoospermic cases. J. Exp. Clin. Med. 2021;38:272–276.
MLA Altun, Ayşe vd. “Ultrastructural Examination of Testicular Tissues and Evaluation of ZO-1 Protein Levels Obtained from Azoospermic Cases”. Journal of Experimental and Clinical Medicine, c. 38, sy. 3, 2021, ss. 272-6.
Vancouver Altun A, Ersoy Canıllıoğlu Y, Unsal E, Karabulut S, Hurdag C. Ultrastructural examination of testicular tissues and evaluation of ZO-1 protein levels obtained from azoospermic cases. J. Exp. Clin. Med. 2021;38(3):272-6.