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Cytotoxic Effect of Laurocerasus officinalis Extract on Human Cancer Cell Lines

Yıl 2017, Cilt: 21 Sayı: 1, 121 - 126, 20.09.2016
https://doi.org/10.12991/marupj.259889

Öz

Laurocerasus officinalis is a popular black summer fruit and it belongs to Prunus genus and Rosaceae family. L. officinalis has many biological activities, such as anti-inflammatory, antinociceptive, antioxidant, neuroprotective, antidiabetic, among others. The aim of the current study was to determine the antioxidant activity and cytotoxic effects of dimethyl sulfoxide extract of L. officinalis (DEL). Total polyphenolic content and cytotoxic activity of DEL was investigated using Folin-Ciocalteu method and MTT assay, respectively. Total polyphenolic content of DEL was found 33.7±0.13 mg gallic acid equivalent/g fruit powder. DEL exhibited selective cytotoxic effect against four human cancer (lung, colon, liver and cervix) cell lines. The predominant selective cytotoxic effect of DEL was observed against colon cancer cells (IC50=265.2±8.1 µg/mL) compared to normal fibroblast cells. These data demonstrate that L. officinalis extract exhibited selective cytotoxicity towards some cancer cells. To clarify the effective molecules and their mechanisms, further studies are needed to undertaken.

Kaynakça

  • Erdemoglu N, Kupeli E, Yesilada E. Anti-inflammatory and antinociceptive activity assessment of plants used as remedy in Turkish folk medicine. J Ethnopharmacol 2003; 89(1): 123-9.
  • Kolayli S, Kucuk M, Duran C, Candan F, Dincer B. Chemical and antioxidant properties of Laurocerasus officinalis Roem. (Cherry laurel) fruit grown in the Black Sea region. J Agric Food Chem 2003; 51(25): 7489-94.
  • Liyana-Pathirana CM, Shahidi F, Alasalvar C. Antioxidant activity of cherry laurel fruit (Laurocerasus officinalis Roem.) and its concentrated juice. Food Chem 2006; 99(1): 121-8.
  • Celep E, Aydin A, Yesilada E. A comparative study on the in vitro antioxidant potentials of three edible fruits: Cornelian cherry, Japanese persimmon and cherry laurel. Food Chem Toxicol 2012; 50(9): 3329-35.
  • Karabegovic IT, Stojicevic SS, Velickovic DT, Nikolic NC, Lazic ML. Optimization of microwave-assisted extraction of cherry laurel fruit. Separ Sci Technol 2014; 49(3): 416-23.
  • Akbulut S, Bayramoglu MM. Reflections of socio-economic and demographic structure of urban and rural on the use of medicinal and aromatic plants: The sample of Trabzon province. Ethno Med 2014; 8(1): 89-100.
  • Senayli A, Sahin A, Senayli Y, Elmastas M. Evaluation the anti-diabetic activity of cherry laurel (Laurocerasus officinalis). Open Conf Proc J 2012; 3: 8-12.
  • Reddy L, Odhav B, Bhoola KD. Natural products for cancer prevention: a global perspective. Pharmacol Ther 2003; 99(1): 1-13.
  • Huang WY, Cai YZ. Natural phenolic compounds from medicinal herbs and dietary plants: potential use for cancer prevention. Nutr Cancer 2010; 62(1): 1-20.
  • Gottesman MM. Mechanism of cancer drug resistance. Annu Rev Med 2002; 53: 615-27.
  • Demir S, Aliyazicioglu Y, Turan I, Misir S, Mentese A, Ozer Yaman S, et al. Antiproliferative and proapoptotic activity of Turkish propolis on human lung cancer cell line. Nutr Cancer 2016; 68(1): 165-72.
  • Nabende PN, Karanja SM, Mwatha JK, Wachira SW. Anti-proliferative activity of Prunus africana, Warburgia stuhlmannii and Maytenus senegalensis extracts in breast and colon cancer cell lines. European J Med Plants 2015; 5(4): 366-76.
  • Poongodi T, Srikanth R, Lalitha G. Phytochemistry, GC-MS analysis and in vitro cytotoxic activity of Prunus angustifolia leaves against MCF-7 breast cancer cell line. WJPPS 2015; 4(10): 1489-99.
  • Noratto G, Porter W, Byrne D, Cisneros-Zevallos C. Polyphenolics from peach (Prunus persica var. Rich Lady) inhibit tumor growth and metastasis of MDA-MB-435 breast cancer cells in vivo. J Nutr Biochem 2014; 25(7): 796-800.
  • Turan I, Demir S, Misir S, Kılınç K, Mentese A, Aliyazicioglu Y, et al. Cytotoxic effect of Turkish propolis on liver, colon, breast, cervix and prostate cancer cell lines. Trop J Pharm Res 2015; 14(5): 777-82.
  • Alasalvar C, Al-Farsi M, Shahidi F. Compositional characteristics and antioxidant components of cherry laurel varieties and pekmez. J Food Sci 2005; 70(1): 47-52.
  • Karahalil FY, Sahin H. Phenolic composition and antioxidant capacity of Cherry laurel (Laurocerasus officinalis Roem.) sampled from Trabzon region, Turkey. Afr J Biotechnol 2011; 10(72): 16293-9.
  • Slinkard K, Singleton VL. Total phenol analyses: automation and comparison with manual methods. Am J Enol Vitic 1977; 28: 49-55.
  • Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983; 65(1-2): 55-63.
  • Dai J, Mumper RJ. Plant phenolics: Extraction, analysis and their antioxidant and anticancer properties. Molecules 2010; 15(10): 7313-52.
  • Vinson JA, Su X, Zubik L, Bose P. Phenol antioxidant quality in food: fruits. J Agr and Food Chem 2001; 49(11): 5315-21.
  • Orhan IE, Akkol EK. Estimation of neuroprotective effects of Laurocerasus officinalis Roem. (cherry laurel) by in vitro methods. Food Res Int 2011; 44(3): 818-22.
  • Lee BB, Cha MR, Kim SY, Park E, Park HR, Lee SC. Antioxidative and anticancer activity of extracts of cherry (Prunus serrulata var. spontanea) blossoms. Plant Foods Hum Nutr 2007; 62: 79-84.
  • Galati G, O’Brien PJ. Potential toxicity of flavonoids and other dietary phenolics: significance for their chemopreventive and anticancer properties. Free Radic Biol Med 2004; 37: 287-303.
  • Fujii T, Ikami T, Xu JW, Ikeda KJ. Prune extract (Prunus domestica L.) suppresses the proliferation and induces the apoptosis of human colon carcinoma Caco-2. Nutr Sci Vitaminol 2006; 52(5): 389-91.
  • Adachi M, Suzuki Y, Mizuta T, Osawa T, Adachi T, Osaka K, et al. The "Prunus mume Sieb. et Zucc" (Ume) is a rich natural source of novel anti-cancer substance. Int J Food Prop 2007; 10: 375-84.
  • Jeong JT, Moon JH, Park KH, Shin CS. Isolation and characterization of a new compound from Prunus mume fruit that inhibits cancer cells. J Agric Food Chem 2006; 54: 2123-8.
  • Sunaga N, Hiraishi K, Ishizuka T, Kaira K, Iwasaki Y, Jimma F, et al. MK615, a compound extract from the Japanese apricot "Prunus mume" inhibits in vitro cell growth and interleukin-8 expression in non-small cell lung cancer cells. J Cancer Sci Ther 2011; S11: 002.
  • Rice-Evans CA, Miller NJ, Paganga G. Antioxidant properties of phenolic compounds. Trends Plant Sci 1997; 2(4): 152-9.
  • Surh YJ. Cancer chemoprevention with dietary phytochemicals. Nat Rev Cancer 2003; 3(10): 768-80.
  • Han XZ, Shen T, Lou HX. Dietary polyphenols and their biological significance. Int J Mol Sci 2007; 8(9): 950-88.
  • Kampa M, Alexaki VI, Notas G, Nifli AP, Nistikaki A, Hatzoglou A, et al. Antiproliferative and apoptotic effects of selective phenolic acids on T47D human breast cancer cells: potential mechanisms of action. Breast Cancer Res 2003; 6(2): 63-74.
  • Feng R, Lu Y, Bowman YL, Qian Y, Castranova V, Ding M. Inhibition of activator protein-1, NF-κB, and MAPKs and induction of phase 2 detoxifying enzyme activity by chlorogenic acid. J Biol Chem 2005; 290(30): 27888-95.
  • Jaganathan SK, Supriyanto E, Mandal M. Events associated with apoptotic effect of p-coumaric acid in HCT-15 colon cancer cells. World J Gastroenterol 2013; 19(43): 7726-34.

Laurocerasus officinalis Ekstraktının İnsan Kanser Hücre Serileri Üzerindeki Sitotoksik Etkisi

Yıl 2017, Cilt: 21 Sayı: 1, 121 - 126, 20.09.2016
https://doi.org/10.12991/marupj.259889

Öz

Laurocerasus officinalis Roem.
(Prunus laurocerasus L.), yerel  olarak
karayemiş veya taflan olarak da bilinir, Prunus cinsi ve  Rosaceae familyasına mensup, koyu renkli bir
yaz meyvesidir.  L. officinalis,
anti-inflamatuvar, antinosiseptif, antioksidan, 
nöroprotektif ve antidiyabetik gibi çeşitli biyolojik aktiviteler  göstermektedir. Bu çalışmanın amacı, L.
officinalis’in dimetil  sülfoksitli
ekstraktının antioksidan ve sitotoksik etkilerini  belirlemektir. Ekstraktın toplam polifenol
içeriği Folin-  Ciocalteu metodu,
sitotoksik etkinliği ise MTT yöntemiyle  belirlendi.
Ekstraktın toplam polifenol içeriği 33.7±0.13 mg  gallik asit ekivalanı/g örnek olarak bulundu.
Ekstrakt akciğer,  kolon, karaciğer ve
serviks kanser hücre serilerinde seçici  bir
sitotoksik etki gösterdi. Normal fibroblast hücreleri ile  karşılaştırıldığında en etkili seçici
sitotoksik etkinin kolon  kanseri
hücrelerinde olduğu belirlendi (IC50=265.2±8.1 μg/mL).  Sonuçlar L. officinalis ekstraktının bazı
kanser hücre serilerinde  seçici bir
sitotoksik etki gösterebildiğini ortaya koymaktadır.  Ekstrakttaki etken molekülleri ve bunların
etki mekanizmalarını  belirleyebilmek
için daha ileri çalışmalara ihtiyaç vardır.  

Kaynakça

  • Erdemoglu N, Kupeli E, Yesilada E. Anti-inflammatory and antinociceptive activity assessment of plants used as remedy in Turkish folk medicine. J Ethnopharmacol 2003; 89(1): 123-9.
  • Kolayli S, Kucuk M, Duran C, Candan F, Dincer B. Chemical and antioxidant properties of Laurocerasus officinalis Roem. (Cherry laurel) fruit grown in the Black Sea region. J Agric Food Chem 2003; 51(25): 7489-94.
  • Liyana-Pathirana CM, Shahidi F, Alasalvar C. Antioxidant activity of cherry laurel fruit (Laurocerasus officinalis Roem.) and its concentrated juice. Food Chem 2006; 99(1): 121-8.
  • Celep E, Aydin A, Yesilada E. A comparative study on the in vitro antioxidant potentials of three edible fruits: Cornelian cherry, Japanese persimmon and cherry laurel. Food Chem Toxicol 2012; 50(9): 3329-35.
  • Karabegovic IT, Stojicevic SS, Velickovic DT, Nikolic NC, Lazic ML. Optimization of microwave-assisted extraction of cherry laurel fruit. Separ Sci Technol 2014; 49(3): 416-23.
  • Akbulut S, Bayramoglu MM. Reflections of socio-economic and demographic structure of urban and rural on the use of medicinal and aromatic plants: The sample of Trabzon province. Ethno Med 2014; 8(1): 89-100.
  • Senayli A, Sahin A, Senayli Y, Elmastas M. Evaluation the anti-diabetic activity of cherry laurel (Laurocerasus officinalis). Open Conf Proc J 2012; 3: 8-12.
  • Reddy L, Odhav B, Bhoola KD. Natural products for cancer prevention: a global perspective. Pharmacol Ther 2003; 99(1): 1-13.
  • Huang WY, Cai YZ. Natural phenolic compounds from medicinal herbs and dietary plants: potential use for cancer prevention. Nutr Cancer 2010; 62(1): 1-20.
  • Gottesman MM. Mechanism of cancer drug resistance. Annu Rev Med 2002; 53: 615-27.
  • Demir S, Aliyazicioglu Y, Turan I, Misir S, Mentese A, Ozer Yaman S, et al. Antiproliferative and proapoptotic activity of Turkish propolis on human lung cancer cell line. Nutr Cancer 2016; 68(1): 165-72.
  • Nabende PN, Karanja SM, Mwatha JK, Wachira SW. Anti-proliferative activity of Prunus africana, Warburgia stuhlmannii and Maytenus senegalensis extracts in breast and colon cancer cell lines. European J Med Plants 2015; 5(4): 366-76.
  • Poongodi T, Srikanth R, Lalitha G. Phytochemistry, GC-MS analysis and in vitro cytotoxic activity of Prunus angustifolia leaves against MCF-7 breast cancer cell line. WJPPS 2015; 4(10): 1489-99.
  • Noratto G, Porter W, Byrne D, Cisneros-Zevallos C. Polyphenolics from peach (Prunus persica var. Rich Lady) inhibit tumor growth and metastasis of MDA-MB-435 breast cancer cells in vivo. J Nutr Biochem 2014; 25(7): 796-800.
  • Turan I, Demir S, Misir S, Kılınç K, Mentese A, Aliyazicioglu Y, et al. Cytotoxic effect of Turkish propolis on liver, colon, breast, cervix and prostate cancer cell lines. Trop J Pharm Res 2015; 14(5): 777-82.
  • Alasalvar C, Al-Farsi M, Shahidi F. Compositional characteristics and antioxidant components of cherry laurel varieties and pekmez. J Food Sci 2005; 70(1): 47-52.
  • Karahalil FY, Sahin H. Phenolic composition and antioxidant capacity of Cherry laurel (Laurocerasus officinalis Roem.) sampled from Trabzon region, Turkey. Afr J Biotechnol 2011; 10(72): 16293-9.
  • Slinkard K, Singleton VL. Total phenol analyses: automation and comparison with manual methods. Am J Enol Vitic 1977; 28: 49-55.
  • Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983; 65(1-2): 55-63.
  • Dai J, Mumper RJ. Plant phenolics: Extraction, analysis and their antioxidant and anticancer properties. Molecules 2010; 15(10): 7313-52.
  • Vinson JA, Su X, Zubik L, Bose P. Phenol antioxidant quality in food: fruits. J Agr and Food Chem 2001; 49(11): 5315-21.
  • Orhan IE, Akkol EK. Estimation of neuroprotective effects of Laurocerasus officinalis Roem. (cherry laurel) by in vitro methods. Food Res Int 2011; 44(3): 818-22.
  • Lee BB, Cha MR, Kim SY, Park E, Park HR, Lee SC. Antioxidative and anticancer activity of extracts of cherry (Prunus serrulata var. spontanea) blossoms. Plant Foods Hum Nutr 2007; 62: 79-84.
  • Galati G, O’Brien PJ. Potential toxicity of flavonoids and other dietary phenolics: significance for their chemopreventive and anticancer properties. Free Radic Biol Med 2004; 37: 287-303.
  • Fujii T, Ikami T, Xu JW, Ikeda KJ. Prune extract (Prunus domestica L.) suppresses the proliferation and induces the apoptosis of human colon carcinoma Caco-2. Nutr Sci Vitaminol 2006; 52(5): 389-91.
  • Adachi M, Suzuki Y, Mizuta T, Osawa T, Adachi T, Osaka K, et al. The "Prunus mume Sieb. et Zucc" (Ume) is a rich natural source of novel anti-cancer substance. Int J Food Prop 2007; 10: 375-84.
  • Jeong JT, Moon JH, Park KH, Shin CS. Isolation and characterization of a new compound from Prunus mume fruit that inhibits cancer cells. J Agric Food Chem 2006; 54: 2123-8.
  • Sunaga N, Hiraishi K, Ishizuka T, Kaira K, Iwasaki Y, Jimma F, et al. MK615, a compound extract from the Japanese apricot "Prunus mume" inhibits in vitro cell growth and interleukin-8 expression in non-small cell lung cancer cells. J Cancer Sci Ther 2011; S11: 002.
  • Rice-Evans CA, Miller NJ, Paganga G. Antioxidant properties of phenolic compounds. Trends Plant Sci 1997; 2(4): 152-9.
  • Surh YJ. Cancer chemoprevention with dietary phytochemicals. Nat Rev Cancer 2003; 3(10): 768-80.
  • Han XZ, Shen T, Lou HX. Dietary polyphenols and their biological significance. Int J Mol Sci 2007; 8(9): 950-88.
  • Kampa M, Alexaki VI, Notas G, Nifli AP, Nistikaki A, Hatzoglou A, et al. Antiproliferative and apoptotic effects of selective phenolic acids on T47D human breast cancer cells: potential mechanisms of action. Breast Cancer Res 2003; 6(2): 63-74.
  • Feng R, Lu Y, Bowman YL, Qian Y, Castranova V, Ding M. Inhibition of activator protein-1, NF-κB, and MAPKs and induction of phase 2 detoxifying enzyme activity by chlorogenic acid. J Biol Chem 2005; 290(30): 27888-95.
  • Jaganathan SK, Supriyanto E, Mandal M. Events associated with apoptotic effect of p-coumaric acid in HCT-15 colon cancer cells. World J Gastroenterol 2013; 19(43): 7726-34.
Toplam 34 adet kaynakça vardır.

Ayrıntılar

Konular Sağlık Kurumları Yönetimi
Bölüm Makaleler
Yazarlar

Selim Demir

Ibrahim Turan

Feyza Demir Bu kişi benim

Elif Ayazoglu Demir Bu kişi benim

Yuksel Aliyazicioglu Bu kişi benim

Yayımlanma Tarihi 20 Eylül 2016
Yayımlandığı Sayı Yıl 2017 Cilt: 21 Sayı: 1

Kaynak Göster

APA Demir, S., Turan, I., Demir, F., Ayazoglu Demir, E., vd. (2016). Cytotoxic Effect of Laurocerasus officinalis Extract on Human Cancer Cell Lines. Marmara Pharmaceutical Journal, 21(1), 121-126. https://doi.org/10.12991/marupj.259889
AMA Demir S, Turan I, Demir F, Ayazoglu Demir E, Aliyazicioglu Y. Cytotoxic Effect of Laurocerasus officinalis Extract on Human Cancer Cell Lines. mpj. Eylül 2016;21(1):121-126. doi:10.12991/marupj.259889
Chicago Demir, Selim, Ibrahim Turan, Feyza Demir, Elif Ayazoglu Demir, ve Yuksel Aliyazicioglu. “Cytotoxic Effect of Laurocerasus Officinalis Extract on Human Cancer Cell Lines”. Marmara Pharmaceutical Journal 21, sy. 1 (Eylül 2016): 121-26. https://doi.org/10.12991/marupj.259889.
EndNote Demir S, Turan I, Demir F, Ayazoglu Demir E, Aliyazicioglu Y (01 Eylül 2016) Cytotoxic Effect of Laurocerasus officinalis Extract on Human Cancer Cell Lines. Marmara Pharmaceutical Journal 21 1 121–126.
IEEE S. Demir, I. Turan, F. Demir, E. Ayazoglu Demir, ve Y. Aliyazicioglu, “Cytotoxic Effect of Laurocerasus officinalis Extract on Human Cancer Cell Lines”, mpj, c. 21, sy. 1, ss. 121–126, 2016, doi: 10.12991/marupj.259889.
ISNAD Demir, Selim vd. “Cytotoxic Effect of Laurocerasus Officinalis Extract on Human Cancer Cell Lines”. Marmara Pharmaceutical Journal 21/1 (Eylül 2016), 121-126. https://doi.org/10.12991/marupj.259889.
JAMA Demir S, Turan I, Demir F, Ayazoglu Demir E, Aliyazicioglu Y. Cytotoxic Effect of Laurocerasus officinalis Extract on Human Cancer Cell Lines. mpj. 2016;21:121–126.
MLA Demir, Selim vd. “Cytotoxic Effect of Laurocerasus Officinalis Extract on Human Cancer Cell Lines”. Marmara Pharmaceutical Journal, c. 21, sy. 1, 2016, ss. 121-6, doi:10.12991/marupj.259889.
Vancouver Demir S, Turan I, Demir F, Ayazoglu Demir E, Aliyazicioglu Y. Cytotoxic Effect of Laurocerasus officinalis Extract on Human Cancer Cell Lines. mpj. 2016;21(1):121-6.