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Manyetik Alanın Pleurotus ostreatus (Jacq. ex Fr.) P. Kumm. ve Lentinula edodes (Berk.) Pegler 'in Misel Büyüme Hızına Etkisi

Yıl 2022, Cilt: 13 Sayı: 1, 84 - 91, 28.04.2022

Öz

Bu çalışmada, manyetik alan uygulamasının istiridye (Pleurotus ostreatus) ve şitake (Lentinula edodes) mantarları misel büyüme hızına etkileri incelenmiştir. 121°C'de 30 dakika otoklavlanan 30 ml, %2 Malt Extract Agar (MEA), 9 cm çapında sterilize edilmiş petri kaplarına dökülmüştür. Daha sonra, her iki mantarın miselleri steril koşullar altında petri kaplarına ekilmiş ve bir solenoid kullanılarak farklı sürelerde (5, 10, 15 ve 30 dak.) 0.25, 0.5, 1, 5, 10, 50 ve 100 mT olmak üzere yedi manyetik alan kuvvetinden birine maruz bırakılmıştır. Kültürler oda sıcaklığında (yaklaşık 23°C) muhafaza edilmiştir. Her iki günde bir, misellerin büyüme oranları 20 günlük bir süre boyunca ölçülmüştür. Farklı manyetik alan güçleri ve uygulama aralıkları için SPSS18 ile iki yönlü varyans analizi yapılmıştır. Manyetik alan ve uygulama süreleri arasındaki farkı göstermek için LSD testi kullanılmıştır. Yapılan analizler sonucunda şu sonuçlar bulunmuştur: 1) Manyetik alan uygulaması, şitake mantarının misel büyüme hızı üzerinde önemli bir etkiye sahip olmuştur, ancak maruz kalma süresi ve manyetik alan-maruz kalma süresi etkileşiminin büyüme üzerinde herhangi bir etkisi görülmemiştir. Bu çalışmada, şitake mantarı misellerinin gelişimi için 5 mT ve 10 mT manyetik alan maruziyetinin etkili olabileceği sonucuna varılmıştır. 2) İstiridye mantarının misel büyüme hızı, manyetik alan, maruz kalma süresi ve manyetik alan-maruz kalma süresi etkileşiminden önemli ölçüde etkilenmemiştir.

Teşekkür

Bu makale Kastamonu Üniversitesi Fen Bilimleri Enstitüsünde yapılan "Elektromanyetik uygulamaların shiitake ve istiridye mantarının gelişimine etkisi" konulu yüksek lisans tezinden hazırlanmıştır.

Kaynakça

  • Bhatnagar, D. and Deb, A. R. (1977). Some Aspects of Regermination Exposure of Wheat Seeds to Magnetic Field: Germination and Early Growth. Seed Res., 5(2) 129-137.
  • Chittaragi, A., Kumar, A., and Muniraju, K. M. (2018). Evaluation of Various Lignocellulosic Products for the Cultivation of Shiitake Mushroom [Lentinula edodes (Berk.) Pegler]. Int. J. Curr. Microbiol. App. Sci, 7(4) 2199-2203.
  • Debeaujon, I., and Koornneef, M. (2000). Gibberellin Requirement for Arabidopsis Seed Germination Is Determined both by Testa Characteristics and Embryonic Abscisic Acid. Plant Physiology, 122(2) 415-424.
  • Eşitken, A. (2003). Effects of Magnetic Fields on Yield and Growth in Strawberry ‘Camarosa’. The Journal of Horticultural Science and Biotechnology, 78(2) 145-147.
  • Florez, M., Carbonell, M. V., and Martínez, E. (2007). Exposure of Maize Seeds to Stationary Magnetic Fields: Effects On Germination and Early Growth. Environmental and Experimental Botany, 59(1) 68-75.
  • Jamil, Y., ul Haq, Z., Iqbal, M., Perveen, T., and Amin, N. (2012). Enhancement in growth and yield of mushroom using magnetic field treatment. Int Agrophys 26 375–380.
  • Leatham, G. F. (1986). The Ligninolytic Activities of Lentinus edodes and Phanerochaete chrysosporium. Applied Microbiology and Biotechnology, 24(1) 51-58.
  • Marks, N., and Szecówka, P. S. (2010). Impact of Variable Magnetic Field Stimulation on Growth of Aboveground Parts of Potato Plants. International Agrophysics, 24(2) 165-170.
  • Mosa I. A. S., Akkuzu E., Ünal S. and Ugış A. (2018). Effect of Magnetic Field Intensity and Exposure Time on the Mycelial Growth of Pleurotus ostreatus and Pleurotus pulmonarius. International Journal of Science and Research, 7(11) 436-439.
  • Nagy, P., and Fischl, G. (2002). Effect of UV and Visible Light Irradiation on Mycelial Growth and Sclerotium Formation of Sclerotinia sclerotiorum. Acta Phytopathologica et Entomologica Hungarica, 37(1-3) 83-89.
  • Owen, R. D. (1998). MYC mRNA Abundance is Unchanged in Subcultures of HL60 Cells Exposed To Power-Line Frequency Magnetic Fields. Radiation Research, 150(1) 23-30.
  • Öder, N. (1986). Karadeniz Bölgesinde (Sinop-Artvin İlleri arası) Yetişen Önemli Bazı Zehirli Mantarlar Üzerinde Taksonomik Araştırmalar. Selçuk Üniv. Fen-Ed. F. Fen Dergisi, 5 87-104.
  • Peláez, J. C., Torres, C., and Díaz, J. E. (2013). Effect of Two Magnetic Fields on The Mycelial Growth and Sensorial Properties of The Mushroom Pleurotus ostreatus. Revista Colombiana de Ciencias Hortícolas, 7(1) 89-97.
  • Piacentini, M. P., Fraternale, D., Piatti, E., Ricci, D., Vetrano, F., Dachà, M., and Accorsi, A. (2001). Senescence Delay and Change of Antioxidant Enzyme Levels in Cucumis sativus L. Etiolated Seedlings by ELF Magnetic Fields. Plant Science, 161(1) 45-53.
  • Ružič, R., Gogala, N., and Jerman, I. (1997). Sinusoidal Magnetic Fields: Effects on the Growth and Ergosterol Content in Mycorrhizal Fungi. Electro-and Magnetobiology, 16(2) 129-142.
  • Sesli, E., Asan, A. ve Selçuk, F. (edlr.) Abacı Günyar, Ö., Akata, I., Akgül, H., Aktaş, S., Alkan, S., Allı, H., Aydoğdu, H., Berikten,D., Demirel,K., Demirel,R., Doğan,H.H., Erdoğdu,M., Ergül,C.C., Eroğlu,G., Giray,G., Halikî Uztan,A., Kabaktepe,Ş., Kadaifçiler,D., Kalyoncu,F., Karaltı,İ., Kaşık,G., Kaya,A., Keleş,A., Kırbağ,S., Kıvanç,M., Ocak,İ., Ökten,S., Özkale,E., Öztürk,C., Sevindik,M., Şen,B., Şen,İ., Türkekul,İ., Ulukapı,M., Uzun,Ya., Uzun,Yu. and Yoltaş, A. (2020).Türkiye Mantarları Listesi. İstanbul: Ali Nihat Gökyiğit Vakfı Yayını.
  • Shams, G., Ranjbar, M., Abbasi, A. R., Khodarahmpour, Z., Feizi, H., and Zare, R. (2013). Influence of Homogeneous Magnetic Field on the Content of Ten Trace Elements in Stipe and Cap Oyster Mushroom (Pleurotus florida). International Research Journal of Applied and Basic Sciences, 4(5) 1071-1077.
  • Slee, R. W. (1991). The Potential of Small Woodlands in Britain for Edible Mushroom Production. Scottish Forestry, 45(1) 3-12.
  • Tesfaw, A., Tadesse, A., and Kiros, G. (2015). Optimization of Oyster (Pleurotus ostreatus) Mushroom Cultivation Using Locally Available Substrates and Materials in Debre Berhan, Ethiopia. Journal of Applied Biology and Biotechnology, 3(1) 15-20.
  • Yıldız, S., Yıldız, Ü. C., Gezer, E. D., and Temiz, A. (2002). Some Lignocellulosic Wastes Used as Raw Material in Cultivation of the Pleurotus ostreatus Culture Mushroom. Process Biochemistry, 38(3) 301-306.
  • Zembron-Lacny, A., Gajewski, M., Naczk, M., and Siatkowski, I. (2013). Effect of Shiitake (Lentinus edodes) Extract nn Antioxidant and Inflammatory Response to Prolonged Eccentric Exercise. J Physiol Pharmacol, 64(2) 249-254.

Influence of Magnetic Field on the Mycelial Growth Rate of Pleurotus ostreatus (Jacq. ex Fr.) P. Kumm. and Lentinula edodes (Berk.) Pegler

Yıl 2022, Cilt: 13 Sayı: 1, 84 - 91, 28.04.2022

Öz

The effects of magnetic field application on the mycelial growth rate of oyster (Pleurotus ostreatus) and shiitake (Lentinula edodes) mushrooms were studied. 30 ml, %2 Malt Extract Agar (MEA) which autoclaved at 121°C for 30 minutes was placed in 9 cm diameter sterilized Petri dishes. Then, the mycelia of both fungi were planted in the Petri dishes under sterilized conditions, and magnetically exposed to one of seven magnetic field strengths, 0.25, 0.5, 1, 5, 10, 50, and 100 mT for different periods of time (5, 10, 15, and 30 min) using a solenoid., The cultures were conserved at room temperature (approximately 23°C). Once every two days, the growth rates of the mycelia were measured for a period of 20 days. A two-way analysis of variance was performed with SPSS18 for different magnetic field strengths and intervals of application. The LSD test was performed to show the differences. The following results were found: 1) Magnetic field application had a significant effect on the mycelial growth rate of shiitake mushroom, but exposure time and magnetic field-exposure time interaction had not any influence on the growth. In this study, it was concluded that 5 mT and 10 mT magnetic field exposure may be efficient for the development of shiitake mushroom mycelia. 2) Mycelial growth rate of oyster mushroom was not significantly affected by a magnetic field, exposure time, and magnetic field-exposure time interaction.

Kaynakça

  • Bhatnagar, D. and Deb, A. R. (1977). Some Aspects of Regermination Exposure of Wheat Seeds to Magnetic Field: Germination and Early Growth. Seed Res., 5(2) 129-137.
  • Chittaragi, A., Kumar, A., and Muniraju, K. M. (2018). Evaluation of Various Lignocellulosic Products for the Cultivation of Shiitake Mushroom [Lentinula edodes (Berk.) Pegler]. Int. J. Curr. Microbiol. App. Sci, 7(4) 2199-2203.
  • Debeaujon, I., and Koornneef, M. (2000). Gibberellin Requirement for Arabidopsis Seed Germination Is Determined both by Testa Characteristics and Embryonic Abscisic Acid. Plant Physiology, 122(2) 415-424.
  • Eşitken, A. (2003). Effects of Magnetic Fields on Yield and Growth in Strawberry ‘Camarosa’. The Journal of Horticultural Science and Biotechnology, 78(2) 145-147.
  • Florez, M., Carbonell, M. V., and Martínez, E. (2007). Exposure of Maize Seeds to Stationary Magnetic Fields: Effects On Germination and Early Growth. Environmental and Experimental Botany, 59(1) 68-75.
  • Jamil, Y., ul Haq, Z., Iqbal, M., Perveen, T., and Amin, N. (2012). Enhancement in growth and yield of mushroom using magnetic field treatment. Int Agrophys 26 375–380.
  • Leatham, G. F. (1986). The Ligninolytic Activities of Lentinus edodes and Phanerochaete chrysosporium. Applied Microbiology and Biotechnology, 24(1) 51-58.
  • Marks, N., and Szecówka, P. S. (2010). Impact of Variable Magnetic Field Stimulation on Growth of Aboveground Parts of Potato Plants. International Agrophysics, 24(2) 165-170.
  • Mosa I. A. S., Akkuzu E., Ünal S. and Ugış A. (2018). Effect of Magnetic Field Intensity and Exposure Time on the Mycelial Growth of Pleurotus ostreatus and Pleurotus pulmonarius. International Journal of Science and Research, 7(11) 436-439.
  • Nagy, P., and Fischl, G. (2002). Effect of UV and Visible Light Irradiation on Mycelial Growth and Sclerotium Formation of Sclerotinia sclerotiorum. Acta Phytopathologica et Entomologica Hungarica, 37(1-3) 83-89.
  • Owen, R. D. (1998). MYC mRNA Abundance is Unchanged in Subcultures of HL60 Cells Exposed To Power-Line Frequency Magnetic Fields. Radiation Research, 150(1) 23-30.
  • Öder, N. (1986). Karadeniz Bölgesinde (Sinop-Artvin İlleri arası) Yetişen Önemli Bazı Zehirli Mantarlar Üzerinde Taksonomik Araştırmalar. Selçuk Üniv. Fen-Ed. F. Fen Dergisi, 5 87-104.
  • Peláez, J. C., Torres, C., and Díaz, J. E. (2013). Effect of Two Magnetic Fields on The Mycelial Growth and Sensorial Properties of The Mushroom Pleurotus ostreatus. Revista Colombiana de Ciencias Hortícolas, 7(1) 89-97.
  • Piacentini, M. P., Fraternale, D., Piatti, E., Ricci, D., Vetrano, F., Dachà, M., and Accorsi, A. (2001). Senescence Delay and Change of Antioxidant Enzyme Levels in Cucumis sativus L. Etiolated Seedlings by ELF Magnetic Fields. Plant Science, 161(1) 45-53.
  • Ružič, R., Gogala, N., and Jerman, I. (1997). Sinusoidal Magnetic Fields: Effects on the Growth and Ergosterol Content in Mycorrhizal Fungi. Electro-and Magnetobiology, 16(2) 129-142.
  • Sesli, E., Asan, A. ve Selçuk, F. (edlr.) Abacı Günyar, Ö., Akata, I., Akgül, H., Aktaş, S., Alkan, S., Allı, H., Aydoğdu, H., Berikten,D., Demirel,K., Demirel,R., Doğan,H.H., Erdoğdu,M., Ergül,C.C., Eroğlu,G., Giray,G., Halikî Uztan,A., Kabaktepe,Ş., Kadaifçiler,D., Kalyoncu,F., Karaltı,İ., Kaşık,G., Kaya,A., Keleş,A., Kırbağ,S., Kıvanç,M., Ocak,İ., Ökten,S., Özkale,E., Öztürk,C., Sevindik,M., Şen,B., Şen,İ., Türkekul,İ., Ulukapı,M., Uzun,Ya., Uzun,Yu. and Yoltaş, A. (2020).Türkiye Mantarları Listesi. İstanbul: Ali Nihat Gökyiğit Vakfı Yayını.
  • Shams, G., Ranjbar, M., Abbasi, A. R., Khodarahmpour, Z., Feizi, H., and Zare, R. (2013). Influence of Homogeneous Magnetic Field on the Content of Ten Trace Elements in Stipe and Cap Oyster Mushroom (Pleurotus florida). International Research Journal of Applied and Basic Sciences, 4(5) 1071-1077.
  • Slee, R. W. (1991). The Potential of Small Woodlands in Britain for Edible Mushroom Production. Scottish Forestry, 45(1) 3-12.
  • Tesfaw, A., Tadesse, A., and Kiros, G. (2015). Optimization of Oyster (Pleurotus ostreatus) Mushroom Cultivation Using Locally Available Substrates and Materials in Debre Berhan, Ethiopia. Journal of Applied Biology and Biotechnology, 3(1) 15-20.
  • Yıldız, S., Yıldız, Ü. C., Gezer, E. D., and Temiz, A. (2002). Some Lignocellulosic Wastes Used as Raw Material in Cultivation of the Pleurotus ostreatus Culture Mushroom. Process Biochemistry, 38(3) 301-306.
  • Zembron-Lacny, A., Gajewski, M., Naczk, M., and Siatkowski, I. (2013). Effect of Shiitake (Lentinus edodes) Extract nn Antioxidant and Inflammatory Response to Prolonged Eccentric Exercise. J Physiol Pharmacol, 64(2) 249-254.
Toplam 21 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Bölüm ARAŞTIRMA MAKALESİ
Yazarlar

Sabri Ünal 0000-0002-3026-0597

Fatma Sarıtaş 0000-0002-9241-490X

Aybaba Hançerlioğulları 0000-0001-7008-480X

Mertcan Karadeniz 0000-0002-3627-9424

Erol Akkuzu 0000-0003-2533-9759

Yayımlanma Tarihi 28 Nisan 2022
Yayımlandığı Sayı Yıl 2022 Cilt: 13 Sayı: 1

Kaynak Göster

APA Ünal, S., Sarıtaş, F., Hançerlioğulları, A., Karadeniz, M., vd. (2022). Influence of Magnetic Field on the Mycelial Growth Rate of Pleurotus ostreatus (Jacq. ex Fr.) P. Kumm. and Lentinula edodes (Berk.) Pegler. Mantar Dergisi, 13(1), 84-91. https://doi.org/10.30708/mantar.1024385

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