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Micrococcus luteus’un Bazı Gram pozitif Balık Patojenlerine Karşı Etkisinin Araştırılması

Yıl 2016, Cilt: 9 Sayı: 2, 74 - 79, 01.06.2016

Tülay Akaylı Çiğdem Ürkü Özgür Çanak Ece Sönmez M.hakan Erk

Öz

Micrococcus luteus, balık sağlığında bakteriyel hastalıklardan korunmak için probiyotik olarak kullanılan Gram pozitif bir bakteridir. Bu çalışma ile M. luteus’ un kültürü yapılan gökkuşağı alabalıklarında (Oncorhynchus mykiss) hastalığa neden olan Gram pozitif bakterilerin kontrolünde kullanılabilirliğinin in vitro koşullarda araştırılması amaçlanmıştır. Patojen mikroorganizma olarak yurdumuzdaki hasta gökkuşağı alabalıklarından izole edilen Staphylococcus cohnii subsp. cohnii, Staphylococcus epidermidis, Staphylococcus aureus, Lactococcus garvieae ve Enterococcus faecalis gibi Gram pozitif bakteriler kullanılmıştır. Gökkuşağı alabalıklarında patojenite testi yapılan ve kültürü yapılan sinarit balıklarından (Dentex dentex) izole edilen M. luteus’ un bu bakterilere karşı antagonistik etkisi disk difüzyon yöntemi ile test edilmiş ve bu bakterinin S. aureus, S.epidermidis, ve S. cohnii subsp. cohnii’ye karşı geniş inhibisyon zonları oluşturduğu gözlenirken L. garvieae ve E. faecalis’e karşı daha az etki gösterdiği dikkati çekmiştir. Bu çalışma ile ilk kez M. luteus’ un Gram–pozitif bakterilerden olan Staphylococcus türlerine karşı antagonistik etki gösterdiği tespit edilmiştir.

Anahtar Kelimeler

Antagonistik Etki Enterococcus Faecalis Lactococcus Garvieae Micrococcus Luteus Probiyotik Gökkuşağı Alabalığı Staphylococcus Spp.

Kaynakça

  • Abd El-Rhman AM, Khattab YA, Shalaby AM. Micrococcus luteus and Pseudomonas species as probiotics for promoting the growth performance and health of Nile tilapia Oreochromis niloticus. Fish & Shellfish Immun. 2009; 27:175–180.
  • Akaylı T, Ürkü Ç, Başaran B. Kültür balıklarında Staphylococcus cohnii subsp. cohnii enfeksiyonu. İ.Ü. Su Ürün Derg.2011; 26: 1-12.
  • Aoki T, Watanabe T. Studies of drug-resistant bacteria isolated from eel-pond water and intestinal tracts of the eel Anguilla japonica and Anguilla anguilla. B Jpn Soc Scı Fish. 1973; 39: 121–130.
  • Arda M, Seçer S, Sarıeyyüpoğlu M. Balık Hastalıkları. Medisan Yayınları, Ankara.2005.
  • Arığ N, Süzer C, Gökvardar A, Başaran F, Çoban D, Yıldırım Ş, Kamacı HO, Fırat K, Saka Ş. Effects of probiotic (Bacillus sp.) supplementation during larval development of gilthead sea bream (Sparus aurata, L.). Turk J Fish Aquat Sc. 2013; 13:407-414.
  • Austin B, Austin DA. Bacterial FishPathogens: Disease of farmed and wildfish, 5th edition. Springer, New York, 2012.
  • Austin B, Stobie M. Recovery of Micrococcus luteus and presumptive Planococcus sp. from moribund fish during an outbreak of rainbow trout, Oncorhynchus mykiss (Walbaum), fry syndrome in England. J Fish Dis. 1992; 15: 203-206.
  • Austin B. Novel pharmaceutical compounds from marine bacteria. J Appl Bacteriol. 1989; 67: 461-470.
  • Bahadır Koca S, Didinen BI, Ekici S, Dulluç A. Su ürünleri yetiştiriciliğinde probiyotik uygulamaları. Journal of FisheriesSciences.com. 2011; 5: 326-335.
  • Bernan VS, Greenstein M, Maisese WM. Marine microorganisms as a source of new natural products. Adv Appl Microbiol.1997; 43: 57-90.
  • Bhunia AK, Johnson MC, Ray B. Purification, characterization and antimicrobial spectrum of a bacteriocin produced by Pediococcus acidolactici. J Appl Bacteriol.1998; 65: 261–268.
  • Bruun MS, Schmidt AS, Madsen L, Dalsgaard I. Antimicrobial resistance patterns in Danish isolates of Flavobacterium psychrophilum. Aquaculture. 2000; 187: 201–212.
  • Buller NB. Bacteria from fishand other aquatic animals: A practical identification manual. CABI Publishing, UK. 2004.
  • Chantharasophon K, Warong T, Mapatsa P, Leelavatcharamas V. High potential probiotic Bacillus species from gastro-intestinal tract of Nile tilapia (Oreochromis niloticus). Biotechnology . 2011; 6: 498-505.
  • Çağırgan H. Biotyping of Lactococcus garvieae isolated from Turkey. Ege Üniversitesi Journal of Fisheries &Aquatic Science. 2004; 3: 267-269.
  • Çapkın E, Altınok I. Effects of dietary probiotic supplementations on prevention ⁄ treatment of yersiniosis disease. J Appl Microbiol. 2009; 106: 1147-1153.
  • Didinen BI, Metin S, Çayli Ö, Ersoy AT. Screening for candidate probiotic bacteria for the control of Vibrio anguillarum in rainbow trout (Oncorhynchus mykiss, Walbaum). Journal of FisheriesSciences.com. 2013; 4: 317-322.
  • Diler O, Altun S, Adiloglu A, Kubilay A, Isıklı B. First occurance of streptococcosis affecting farmed rainbow trout in Turkey, B Eur Assoc Fish Pat. 2002; 1: 21-26.
  • Eldar A, Bejerano Y, Livoff A, Horovitz A, Bercovier H. Experimental streptococcal meningoencephalitis in cultured fish. Vet Microbiol. 1995; 43: 33- 40.
  • Gauthier MJ, Shewan JM, Gibson DM, Lee JV. Taxonomic position and seasonal variations in marine neritic environment of some Gram-negative antibiotic producing bacteria. J Gen Microbiol. 1995; 87: 211-218.
  • Gil P, Vivas J, Gallardo CS, Rodriguez LA. First isolation of Staphylococcus warneri, from diseased rainbow trout, Oncorhynchus mykiss (Walbaum), in Northwest Spain. J Fish Dis. 2000; 4: 295–298.
  • Gomez RG, Balcazar JL, Shen MA. Probiotics as control agents in aquaculture. Oceanic and Coastal Sea Research. 2007; 1: 76-79.
  • Irianto A, Austin B. Probiotics in aquaculture: Review. J Fish Dis. 2002b; 25: 633–642.
  • Irianto A, Austin B. Use of probiotics to control furunculosis in rainbow trout,Oncorhynchus mykiss (Walbaum). J Fish Diseases. 2002a; 25: 333–34.
  • Korkut AY, Hoşsu B, Ferhatoğlu M. Probiyotikler ve su ürünlerinde kullanımı. Ege Üniversitesi Su Ürün Derg. 2003; 20: 551 – 556.
  • Miranda CD, Zemelman R. Bacterial resistance to oxytetracycline in Chilean salmon farming. Aquaculture. 2002; 212: 31–47.
  • Mousavi SS, Khara H, Saeidi AA, Ghiasi M, Zahedi A. Determination of staphylococcosis and micrococcosis outbreak on selected rainbow trout farms in Mazandaran Province. J Fish. 2010; 1: 109-114.
  • OIE (Office International des Epizooties). Antimicrobial use in aquaculture and antimicrobial resistance. report of a joint FAO/OIE/WHO expert consultation. Seoul, Republic of Korea, 13- 16 June, WHO Document Production Services, Geneva, Switzerland. 2006.
  • Özer S, Bulduklu S, Dönmez E. Streptococcocis occurence at rainbow trout (Oncorhynchus mykiss, Walbaum) cultivated in province Mersin-Turkey, Journal of Fisheries Sciences.com. 2008; 3: 272-283.
  • Savaşan S, Kaya O, Kırkan Ş, Çiftçi A. Balık kökenli Enterococcus faecalis suşlarının antibiyotik dirençlilikleri. A.Ü. Vet Fak Derg. 2008; 55: 107-110.
  • Süzer C, Çoban D, Kamacı OH, Saka S, Fırat K, Otgucuoglu O, Küçüksarı H. Lactobacillus spp. bacteria as probiotics in gilthead sea bream (Sparus aurata, L.) larvae: effects on growth performance and digestive enzyme activities. Aquaculture . 2008; 280: 140-145.
  • Timur G, Akayli T. First study of staphylococcosis in farmed rainbow trout fry (Oncorhyncus mykiss W.1792) in Turkey. International Symposium of Fisheries and Zoology, October 23 -26, İstanbul, 2003, pp 67-80.
  • Timur G, Yardımcı RE, Ürkü Ç, Çanak Ö. Marmara bölgesi kültür alabalıklarında (Oncorhynchus mykiss, W.) lactococcosis’ in bakteriyolojik ve histopatolojik metodlarla teşhisi. İ.Ü. Su Ürün Derg. 2011; 26: 63-81.

The investigation of Micrococcus luteus Against Some Gram positive Fish Bacteria

Yıl 2016, Cilt: 9 Sayı: 2, 74 - 79, 01.06.2016

Tülay Akaylı Çiğdem Ürkü Özgür Çanak Ece Sönmez M.hakan Erk

Öz

Micrococcus luteus is a Gram positive bacterium that is used as probiotic for the prevention of bacterial diseases in fish health. The aim of this study is the determination of probiotic suitability of M. luteus in the control of Gram positive bacteria which cause diseases in the cultured rainbow trout (Oncorhynchus mykiss) under in vitro conditions. Gram positive bacteria namely Staphylococcus cohnii subsp. cohnii, Staphylococcus epidermidis, Staphylococcus aureus, Lactococcus garvieae ve Enterococcus faecalis which were isolated from rainbow trout, were used as pathogen microorganisms. The antagonistic effect of M. luteus isolated from cultured dentex (Dentex dentex) and made pathogenity test for cultured rainbow trout against these bacteria was tested by using disc diffusion method and while M. luteus produced wide inhibition zones against S. aureus, S.epidermidis and S.cohnii subsp. cohnii, less antagonistic effect was determined against L. garvieae and E. faecalis. With this study, antagonistic effect of M. luteus against members of the Gram positive bacterial genus Staphylococcus was determined for the first time.

Anahtar Kelimeler

Antagonistic Effect Enterococcus Faecalis Lactococcus Garvieae Micrococcus Luteus Probiotic Rainbow Trout Staphylococcus Spp.

Kaynakça

  • Abd El-Rhman AM, Khattab YA, Shalaby AM. Micrococcus luteus and Pseudomonas species as probiotics for promoting the growth performance and health of Nile tilapia Oreochromis niloticus. Fish & Shellfish Immun. 2009; 27:175–180.
  • Akaylı T, Ürkü Ç, Başaran B. Kültür balıklarında Staphylococcus cohnii subsp. cohnii enfeksiyonu. İ.Ü. Su Ürün Derg.2011; 26: 1-12.
  • Aoki T, Watanabe T. Studies of drug-resistant bacteria isolated from eel-pond water and intestinal tracts of the eel Anguilla japonica and Anguilla anguilla. B Jpn Soc Scı Fish. 1973; 39: 121–130.
  • Arda M, Seçer S, Sarıeyyüpoğlu M. Balık Hastalıkları. Medisan Yayınları, Ankara.2005.
  • Arığ N, Süzer C, Gökvardar A, Başaran F, Çoban D, Yıldırım Ş, Kamacı HO, Fırat K, Saka Ş. Effects of probiotic (Bacillus sp.) supplementation during larval development of gilthead sea bream (Sparus aurata, L.). Turk J Fish Aquat Sc. 2013; 13:407-414.
  • Austin B, Austin DA. Bacterial FishPathogens: Disease of farmed and wildfish, 5th edition. Springer, New York, 2012.
  • Austin B, Stobie M. Recovery of Micrococcus luteus and presumptive Planococcus sp. from moribund fish during an outbreak of rainbow trout, Oncorhynchus mykiss (Walbaum), fry syndrome in England. J Fish Dis. 1992; 15: 203-206.
  • Austin B. Novel pharmaceutical compounds from marine bacteria. J Appl Bacteriol. 1989; 67: 461-470.
  • Bahadır Koca S, Didinen BI, Ekici S, Dulluç A. Su ürünleri yetiştiriciliğinde probiyotik uygulamaları. Journal of FisheriesSciences.com. 2011; 5: 326-335.
  • Bernan VS, Greenstein M, Maisese WM. Marine microorganisms as a source of new natural products. Adv Appl Microbiol.1997; 43: 57-90.
  • Bhunia AK, Johnson MC, Ray B. Purification, characterization and antimicrobial spectrum of a bacteriocin produced by Pediococcus acidolactici. J Appl Bacteriol.1998; 65: 261–268.
  • Bruun MS, Schmidt AS, Madsen L, Dalsgaard I. Antimicrobial resistance patterns in Danish isolates of Flavobacterium psychrophilum. Aquaculture. 2000; 187: 201–212.
  • Buller NB. Bacteria from fishand other aquatic animals: A practical identification manual. CABI Publishing, UK. 2004.
  • Chantharasophon K, Warong T, Mapatsa P, Leelavatcharamas V. High potential probiotic Bacillus species from gastro-intestinal tract of Nile tilapia (Oreochromis niloticus). Biotechnology . 2011; 6: 498-505.
  • Çağırgan H. Biotyping of Lactococcus garvieae isolated from Turkey. Ege Üniversitesi Journal of Fisheries &Aquatic Science. 2004; 3: 267-269.
  • Çapkın E, Altınok I. Effects of dietary probiotic supplementations on prevention ⁄ treatment of yersiniosis disease. J Appl Microbiol. 2009; 106: 1147-1153.
  • Didinen BI, Metin S, Çayli Ö, Ersoy AT. Screening for candidate probiotic bacteria for the control of Vibrio anguillarum in rainbow trout (Oncorhynchus mykiss, Walbaum). Journal of FisheriesSciences.com. 2013; 4: 317-322.
  • Diler O, Altun S, Adiloglu A, Kubilay A, Isıklı B. First occurance of streptococcosis affecting farmed rainbow trout in Turkey, B Eur Assoc Fish Pat. 2002; 1: 21-26.
  • Eldar A, Bejerano Y, Livoff A, Horovitz A, Bercovier H. Experimental streptococcal meningoencephalitis in cultured fish. Vet Microbiol. 1995; 43: 33- 40.
  • Gauthier MJ, Shewan JM, Gibson DM, Lee JV. Taxonomic position and seasonal variations in marine neritic environment of some Gram-negative antibiotic producing bacteria. J Gen Microbiol. 1995; 87: 211-218.
  • Gil P, Vivas J, Gallardo CS, Rodriguez LA. First isolation of Staphylococcus warneri, from diseased rainbow trout, Oncorhynchus mykiss (Walbaum), in Northwest Spain. J Fish Dis. 2000; 4: 295–298.
  • Gomez RG, Balcazar JL, Shen MA. Probiotics as control agents in aquaculture. Oceanic and Coastal Sea Research. 2007; 1: 76-79.
  • Irianto A, Austin B. Probiotics in aquaculture: Review. J Fish Dis. 2002b; 25: 633–642.
  • Irianto A, Austin B. Use of probiotics to control furunculosis in rainbow trout,Oncorhynchus mykiss (Walbaum). J Fish Diseases. 2002a; 25: 333–34.
  • Korkut AY, Hoşsu B, Ferhatoğlu M. Probiyotikler ve su ürünlerinde kullanımı. Ege Üniversitesi Su Ürün Derg. 2003; 20: 551 – 556.
  • Miranda CD, Zemelman R. Bacterial resistance to oxytetracycline in Chilean salmon farming. Aquaculture. 2002; 212: 31–47.
  • Mousavi SS, Khara H, Saeidi AA, Ghiasi M, Zahedi A. Determination of staphylococcosis and micrococcosis outbreak on selected rainbow trout farms in Mazandaran Province. J Fish. 2010; 1: 109-114.
  • OIE (Office International des Epizooties). Antimicrobial use in aquaculture and antimicrobial resistance. report of a joint FAO/OIE/WHO expert consultation. Seoul, Republic of Korea, 13- 16 June, WHO Document Production Services, Geneva, Switzerland. 2006.
  • Özer S, Bulduklu S, Dönmez E. Streptococcocis occurence at rainbow trout (Oncorhynchus mykiss, Walbaum) cultivated in province Mersin-Turkey, Journal of Fisheries Sciences.com. 2008; 3: 272-283.
  • Savaşan S, Kaya O, Kırkan Ş, Çiftçi A. Balık kökenli Enterococcus faecalis suşlarının antibiyotik dirençlilikleri. A.Ü. Vet Fak Derg. 2008; 55: 107-110.
  • Süzer C, Çoban D, Kamacı OH, Saka S, Fırat K, Otgucuoglu O, Küçüksarı H. Lactobacillus spp. bacteria as probiotics in gilthead sea bream (Sparus aurata, L.) larvae: effects on growth performance and digestive enzyme activities. Aquaculture . 2008; 280: 140-145.
  • Timur G, Akayli T. First study of staphylococcosis in farmed rainbow trout fry (Oncorhyncus mykiss W.1792) in Turkey. International Symposium of Fisheries and Zoology, October 23 -26, İstanbul, 2003, pp 67-80.
  • Timur G, Yardımcı RE, Ürkü Ç, Çanak Ö. Marmara bölgesi kültür alabalıklarında (Oncorhynchus mykiss, W.) lactococcosis’ in bakteriyolojik ve histopatolojik metodlarla teşhisi. İ.Ü. Su Ürün Derg. 2011; 26: 63-81.
Toplam 33 adet kaynakça vardır.

Ayrıntılar

Bölüm ARAŞTIRMA MAKALESİ
Yazarlar

Tülay Akaylı

Çiğdem Ürkü Bu kişi benim

Özgür Çanak Bu kişi benim

Ece Sönmez Bu kişi benim

M.hakan Erk Bu kişi benim

Yayımlanma Tarihi 1 Haziran 2016
Kabul Tarihi 19 Şubat 2016
Yayımlandığı Sayı Yıl 2016 Cilt: 9 Sayı: 2

Kaynak Göster

APA Akaylı, T., Ürkü, Ç., Çanak, Ö., Sönmez, E., vd. (2016). The investigation of Micrococcus luteus Against Some Gram positive Fish Bacteria. Kocatepe Veterinary Journal, 9(2), 74-79.
AMA Akaylı T, Ürkü Ç, Çanak Ö, Sönmez E, Erk M. The investigation of Micrococcus luteus Against Some Gram positive Fish Bacteria. kvj. Haziran 2016;9(2):74-79.
Chicago Akaylı, Tülay, Çiğdem Ürkü, Özgür Çanak, Ece Sönmez, ve M.hakan Erk. “The Investigation of Micrococcus Luteus Against Some Gram Positive Fish Bacteria”. Kocatepe Veterinary Journal 9, sy. 2 (Haziran 2016): 74-79.
EndNote Akaylı T, Ürkü Ç, Çanak Ö, Sönmez E, Erk M (01 Haziran 2016) The investigation of Micrococcus luteus Against Some Gram positive Fish Bacteria. Kocatepe Veterinary Journal 9 2 74–79.
IEEE T. Akaylı, Ç. Ürkü, Ö. Çanak, E. Sönmez, ve M. Erk, “The investigation of Micrococcus luteus Against Some Gram positive Fish Bacteria”, kvj, c. 9, sy. 2, ss. 74–79, 2016.
ISNAD Akaylı, Tülay vd. “The Investigation of Micrococcus Luteus Against Some Gram Positive Fish Bacteria”. Kocatepe Veterinary Journal 9/2 (Haziran 2016), 74-79.
JAMA Akaylı T, Ürkü Ç, Çanak Ö, Sönmez E, Erk M. The investigation of Micrococcus luteus Against Some Gram positive Fish Bacteria. kvj. 2016;9:74–79.
MLA Akaylı, Tülay vd. “The Investigation of Micrococcus Luteus Against Some Gram Positive Fish Bacteria”. Kocatepe Veterinary Journal, c. 9, sy. 2, 2016, ss. 74-79.
Vancouver Akaylı T, Ürkü Ç, Çanak Ö, Sönmez E, Erk M. The investigation of Micrococcus luteus Against Some Gram positive Fish Bacteria. kvj. 2016;9(2):74-9.
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