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Diagnostic value of Cobas Amplicor MTB and Rotorgene Real Time PCR for tuberculous meningitis: A six-year retrospective study

Yıl 2015, Cilt: 5 Sayı: 4, 156 - 161, 06.02.2016
https://doi.org/10.5799/ahinjs.02.2015.04.0197

Öz

Objective: Tuberculous meningitis (TBM) is the most severe and lethal form of tuberculosis (TB).Bacteriologic confirmation of TBM is difficult and slow. Therefore, most patients receive antituberculosis treatment based only on clinical and cerebrospinal fluid (CSF) characteristics. Rapid diagnosis of TBM is important to decrease morbidity and mortality. The aim of the study was to demonstrate that COBAS Amplicor MTB and Rotorgene Real Time (RT) PCR is a rapid method of diagnosing TBM before and after initiating anti-tuberculosis treatment.

Methods: A retrospective study was conducted between December 2002 and January 2009 in 468 patients with suspected TBM. Clinical specimens were collected from different hospitals in Ankara. All specimens were evaluated by smear microscopy and culture methods with Lowenstein-Jensen (LJ) and MGIT culture system.

Results: Using culture results as a gold standard, the sensitivity, specificity, positive predictive values (PPV), and negative predictive values (NPV) were 71.0%, 98.8%, 97.8% and 75.0%, respectively, for COBAS Amplicor MTB and 80%, 98.9%, 99.0% and 80.0%, respectively, for Rotorgene RT PCR. Statistical analysis showed no significant differences between the COBAS Amplicor MTB and Rotorgene RT PCR (p≥0.05). All isolates were susceptible to isoniazid, rifampin, streptomycin, and ethambutol with proportion method in LJ medium. All isolates were defined as LAM7-TUR by spoligotyping.

Conclusion: Retrospective analysis of COBAS Amplicor MTB and Rotorgene RT PCR found that both tests are effective in rapidly diagnosing MTB using CSF. It was concluded that Rotorgene RT PCR test is more sensitive (81.0%) than COBAS Amplicor MTB (71.0%). J Microbiol Infect Dis 2015;5(4): 156-161

Key words: Tuberculous meningitis, COBAS Amplicor MTB, Rotorgene Real Time PCR

Kaynakça

  • 1. Marx GE, Chan ED. Tuberculous meningitis: diagnosis and treatment overview. Tuberc Res Treat 2011; 2011:798764.
  • 2. Rock RB, Olin M, Baker CA, et al. Central nervous system tuberculosis: pathogenesis and clinical aspects. Clin Microbiol Rev 2008;21:243-261.
  • 3. Thwaites G, Chau TTH, Mai NTH, et al. Tuberculosis meningitis. J. Neurol Neurosurg Psychiatry 2000;68:289-299.
  • 4. Takahashi T, Tamura M, Takasu T. The PCR-Based Diagnosis of Central Nervous System Tuberculosis: Up to Date Tuberculosis Research and Treatment 2012; Volume 2012, Article ID 831292, 17 pages http://dx.doi.org/10.1155/2012/831292
  • 5. Swarnlata P, Shoba K and Khublani TK. A review on TB and its advance diagnostic techniques. International Journal of Pharma and Bio Sciences 2011;2:535-545.
  • 6. Young CS, Kim MJ, Suh JT, Lee HJ. Comparison of Diagnostic Performance of Three Real-Time PCR Kits for Detecting Mycobacterium Species. Yonsei Med J 2011;52:301-306.
  • 7. D’amato RF, Wallman AA, Hochstein LH, et al. Rapid diagnosis of pulmonary tuberculosis by using Roche AMPLICOR Mycobacterium tuberculosis PCR test. J Clin Microbiol 1995;33:1832-1834.
  • 8. Ninet B, Rohner P, Metral C, Auckenthaler R. Assessment of use of the COBAS AMPLICOR system with BACTEC 12B cultures for rapid detection of frequently identified mycobacteria. J Clin Microbiol 1999;37:782-784.
  • 9. Kim TK, Mukhopadhyay A, Gough A, Khoo K-L, et al. Role of clinical judgment in the application of a nucleic acid amplification test for the rapid diagnosis of pulmonary tuberculosis. Chest 2003; 124:902-908.
  • 10. Bonington A, Strang JI, Klapper PE, et al. TB PCR in the early diagnosis of tuberculous meningitis: evaluation of the Roche semi-automated COBAS Amplicor MTB test with reference to the manual Amplicor MTB PCR test. 2000;80:191-196.
  • 11. Chedore P, Jamieson FB. Rapid molecular diagnosis of tuberculous meningitis using the Gen-probe Amplified Mycobacterium Tuberculosis direct test in a large Canadian public health Laboratory. Int J Tuberc Lung Dis 2002;6:913-919.
  • 12. Kent PT, Kubica G. Public Health Mycobacteriology. A guide for the level III Laboratory. Atlanta, Ga: Centers for Disease Control, 1985.
  • 13. Canetti G, Fox W, Khomenko A, et al. Advances in techniques of testing mycobacterial drug sensitivity, and the use of sensitivity tests in tuberculosis control programmes. Bull World Health Organisation 1969;41:21-43.
  • 14. Jungkind D, DiRenzo S, Beavis KG, Silverman NS. Evaluation of automated COBAS AMPLICOR PCR system for detection of several infectious agents and its impact on laboratory management. J Clin Microbiol 1996;34:2778-2783.
  • 15. Kısa O, Tarhan,G, Gunal S, et al. The Distribution of Spoligotyping Defined Genotypic Lineages Among Drug-Resistant M. tuberculosis complex Clinical Isolates in Ankara, Turkey. Plos One 2012;7:e30331.
  • 16. Erdem H, Engin DO, Elaldi N, et al. The microbiological diagnosis of tuberculous meningitis : results of Haydarpasa-I study. Clinical Microbiology and Infection 2014;20:O600-8. doi: 10.1111469-0691.
  • 17. Wei CY, Hwang JJ, Chu CH, Lee CP.Detection and identification of Mycobacterium tuberculosis by nested PCR assays in cerebrospinal fluid samples from patients with suspected tuberculous meningitis. Kaohsiung J Med Sci 1999;15:475- 483.
  • 18. Reischl U, Lehn N, Wolf H, Naumann L. Clinical Evaluation of the Automated COBAS AMPLICOR MTB Assay for Testing Respiratory and Nonrespiratory Specimens. J Clin Microbiol 1998;36:2853-2860.
  • 19. Thwaites GE, Caws M, Chau TT, et al. Comparison of conventional bacteriology with nucleic acid amplification (amplified mycobacterium direct test) for diagnosis of tuberculous meningitis before and after inception of antituberculosis chemotherapy. J Clin Microbiol 2004;42:996-1002.
  • 20. Chedore P, Jamieson FB. Rapid molecular diagnosis of tuberculous meningitis using the Gen-probe Amplified Myco- bacterium Tuberculosis direct test in a large Canadian public health laboratory. Int J Tuberc Lung Dis 2002;6:913-919.
  • 21. Pai M, Flores LL, Pai N, et al. Diagnostic accuracy of nucleic acid amplification tests for tuberculous meningitis: a systematic review and meta-analysis. Lancet Infect Dis 2003;3:633-643.
  • 22. Kisa O, Albay A, Baylan O, et al. Genetic diversity of Mycobacterium tuberculosis isolates at the Military Medical Academy in Ankara, Turkey. Res 2007; Microbiol 158:318-323.
  • 23. Otlu B, Durmaz R, Gunal S, et al. Beijing/W and majör spoligotype families of Mycobacterium tuberculosis strains isolated from tuberculosis patients in Eastern Turkey. New Microbiol 2009;32:255-263.
  • 24. Zozio T, Allix C, Gunal S, et al. Genotyping ofMycobacterium tuberculosis clinical isolates in two cities of Turkey: Description of a new family of genotypes that is phylogeographically specific for Asia Minor. BMC Microbiology 2005;5:44.

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Yıl 2015, Cilt: 5 Sayı: 4, 156 - 161, 06.02.2016
https://doi.org/10.5799/ahinjs.02.2015.04.0197

Öz

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Kaynakça

  • 1. Marx GE, Chan ED. Tuberculous meningitis: diagnosis and treatment overview. Tuberc Res Treat 2011; 2011:798764.
  • 2. Rock RB, Olin M, Baker CA, et al. Central nervous system tuberculosis: pathogenesis and clinical aspects. Clin Microbiol Rev 2008;21:243-261.
  • 3. Thwaites G, Chau TTH, Mai NTH, et al. Tuberculosis meningitis. J. Neurol Neurosurg Psychiatry 2000;68:289-299.
  • 4. Takahashi T, Tamura M, Takasu T. The PCR-Based Diagnosis of Central Nervous System Tuberculosis: Up to Date Tuberculosis Research and Treatment 2012; Volume 2012, Article ID 831292, 17 pages http://dx.doi.org/10.1155/2012/831292
  • 5. Swarnlata P, Shoba K and Khublani TK. A review on TB and its advance diagnostic techniques. International Journal of Pharma and Bio Sciences 2011;2:535-545.
  • 6. Young CS, Kim MJ, Suh JT, Lee HJ. Comparison of Diagnostic Performance of Three Real-Time PCR Kits for Detecting Mycobacterium Species. Yonsei Med J 2011;52:301-306.
  • 7. D’amato RF, Wallman AA, Hochstein LH, et al. Rapid diagnosis of pulmonary tuberculosis by using Roche AMPLICOR Mycobacterium tuberculosis PCR test. J Clin Microbiol 1995;33:1832-1834.
  • 8. Ninet B, Rohner P, Metral C, Auckenthaler R. Assessment of use of the COBAS AMPLICOR system with BACTEC 12B cultures for rapid detection of frequently identified mycobacteria. J Clin Microbiol 1999;37:782-784.
  • 9. Kim TK, Mukhopadhyay A, Gough A, Khoo K-L, et al. Role of clinical judgment in the application of a nucleic acid amplification test for the rapid diagnosis of pulmonary tuberculosis. Chest 2003; 124:902-908.
  • 10. Bonington A, Strang JI, Klapper PE, et al. TB PCR in the early diagnosis of tuberculous meningitis: evaluation of the Roche semi-automated COBAS Amplicor MTB test with reference to the manual Amplicor MTB PCR test. 2000;80:191-196.
  • 11. Chedore P, Jamieson FB. Rapid molecular diagnosis of tuberculous meningitis using the Gen-probe Amplified Mycobacterium Tuberculosis direct test in a large Canadian public health Laboratory. Int J Tuberc Lung Dis 2002;6:913-919.
  • 12. Kent PT, Kubica G. Public Health Mycobacteriology. A guide for the level III Laboratory. Atlanta, Ga: Centers for Disease Control, 1985.
  • 13. Canetti G, Fox W, Khomenko A, et al. Advances in techniques of testing mycobacterial drug sensitivity, and the use of sensitivity tests in tuberculosis control programmes. Bull World Health Organisation 1969;41:21-43.
  • 14. Jungkind D, DiRenzo S, Beavis KG, Silverman NS. Evaluation of automated COBAS AMPLICOR PCR system for detection of several infectious agents and its impact on laboratory management. J Clin Microbiol 1996;34:2778-2783.
  • 15. Kısa O, Tarhan,G, Gunal S, et al. The Distribution of Spoligotyping Defined Genotypic Lineages Among Drug-Resistant M. tuberculosis complex Clinical Isolates in Ankara, Turkey. Plos One 2012;7:e30331.
  • 16. Erdem H, Engin DO, Elaldi N, et al. The microbiological diagnosis of tuberculous meningitis : results of Haydarpasa-I study. Clinical Microbiology and Infection 2014;20:O600-8. doi: 10.1111469-0691.
  • 17. Wei CY, Hwang JJ, Chu CH, Lee CP.Detection and identification of Mycobacterium tuberculosis by nested PCR assays in cerebrospinal fluid samples from patients with suspected tuberculous meningitis. Kaohsiung J Med Sci 1999;15:475- 483.
  • 18. Reischl U, Lehn N, Wolf H, Naumann L. Clinical Evaluation of the Automated COBAS AMPLICOR MTB Assay for Testing Respiratory and Nonrespiratory Specimens. J Clin Microbiol 1998;36:2853-2860.
  • 19. Thwaites GE, Caws M, Chau TT, et al. Comparison of conventional bacteriology with nucleic acid amplification (amplified mycobacterium direct test) for diagnosis of tuberculous meningitis before and after inception of antituberculosis chemotherapy. J Clin Microbiol 2004;42:996-1002.
  • 20. Chedore P, Jamieson FB. Rapid molecular diagnosis of tuberculous meningitis using the Gen-probe Amplified Myco- bacterium Tuberculosis direct test in a large Canadian public health laboratory. Int J Tuberc Lung Dis 2002;6:913-919.
  • 21. Pai M, Flores LL, Pai N, et al. Diagnostic accuracy of nucleic acid amplification tests for tuberculous meningitis: a systematic review and meta-analysis. Lancet Infect Dis 2003;3:633-643.
  • 22. Kisa O, Albay A, Baylan O, et al. Genetic diversity of Mycobacterium tuberculosis isolates at the Military Medical Academy in Ankara, Turkey. Res 2007; Microbiol 158:318-323.
  • 23. Otlu B, Durmaz R, Gunal S, et al. Beijing/W and majör spoligotype families of Mycobacterium tuberculosis strains isolated from tuberculosis patients in Eastern Turkey. New Microbiol 2009;32:255-263.
  • 24. Zozio T, Allix C, Gunal S, et al. Genotyping ofMycobacterium tuberculosis clinical isolates in two cities of Turkey: Description of a new family of genotypes that is phylogeographically specific for Asia Minor. BMC Microbiology 2005;5:44.
Toplam 24 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Bölüm Kısa Rapor
Yazarlar

Gülnur Tarhan

Hülya Şimşek Bu kişi benim

Salih Cesur Bu kişi benim

İsmail Ceyhan Bu kişi benim

Yayımlanma Tarihi 6 Şubat 2016
Yayımlandığı Sayı Yıl 2015 Cilt: 5 Sayı: 4

Kaynak Göster

APA Tarhan, G., Şimşek, H., Cesur, S., Ceyhan, İ. (2016). Diagnostic value of Cobas Amplicor MTB and Rotorgene Real Time PCR for tuberculous meningitis: A six-year retrospective study. Journal of Microbiology and Infectious Diseases, 5(4), 156-161. https://doi.org/10.5799/ahinjs.02.2015.04.0197
AMA Tarhan G, Şimşek H, Cesur S, Ceyhan İ. Diagnostic value of Cobas Amplicor MTB and Rotorgene Real Time PCR for tuberculous meningitis: A six-year retrospective study. J Microbil Infect Dis. Şubat 2016;5(4):156-161. doi:10.5799/ahinjs.02.2015.04.0197
Chicago Tarhan, Gülnur, Hülya Şimşek, Salih Cesur, ve İsmail Ceyhan. “Diagnostic Value of Cobas Amplicor MTB and Rotorgene Real Time PCR for Tuberculous Meningitis: A Six-Year Retrospective Study”. Journal of Microbiology and Infectious Diseases 5, sy. 4 (Şubat 2016): 156-61. https://doi.org/10.5799/ahinjs.02.2015.04.0197.
EndNote Tarhan G, Şimşek H, Cesur S, Ceyhan İ (01 Şubat 2016) Diagnostic value of Cobas Amplicor MTB and Rotorgene Real Time PCR for tuberculous meningitis: A six-year retrospective study. Journal of Microbiology and Infectious Diseases 5 4 156–161.
IEEE G. Tarhan, H. Şimşek, S. Cesur, ve İ. Ceyhan, “Diagnostic value of Cobas Amplicor MTB and Rotorgene Real Time PCR for tuberculous meningitis: A six-year retrospective study”, J Microbil Infect Dis, c. 5, sy. 4, ss. 156–161, 2016, doi: 10.5799/ahinjs.02.2015.04.0197.
ISNAD Tarhan, Gülnur vd. “Diagnostic Value of Cobas Amplicor MTB and Rotorgene Real Time PCR for Tuberculous Meningitis: A Six-Year Retrospective Study”. Journal of Microbiology and Infectious Diseases 5/4 (Şubat 2016), 156-161. https://doi.org/10.5799/ahinjs.02.2015.04.0197.
JAMA Tarhan G, Şimşek H, Cesur S, Ceyhan İ. Diagnostic value of Cobas Amplicor MTB and Rotorgene Real Time PCR for tuberculous meningitis: A six-year retrospective study. J Microbil Infect Dis. 2016;5:156–161.
MLA Tarhan, Gülnur vd. “Diagnostic Value of Cobas Amplicor MTB and Rotorgene Real Time PCR for Tuberculous Meningitis: A Six-Year Retrospective Study”. Journal of Microbiology and Infectious Diseases, c. 5, sy. 4, 2016, ss. 156-61, doi:10.5799/ahinjs.02.2015.04.0197.
Vancouver Tarhan G, Şimşek H, Cesur S, Ceyhan İ. Diagnostic value of Cobas Amplicor MTB and Rotorgene Real Time PCR for tuberculous meningitis: A six-year retrospective study. J Microbil Infect Dis. 2016;5(4):156-61.