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Influence of Vitamin E and Organic Selenium Supplementation on Antioxidant Enzymes Activities in Blood and Egg Samples of Laying Hens

Yıl 2020, Cilt: 10 Sayı: 1, 694 - 701, 01.03.2020
https://doi.org/10.21597/jist.544969

Öz

This experiment was conducted to evaluate the effects of dietary vitamin E (α-tocopherol acetate) and selenium (selenomethionine) and a combination of the two, on the activities of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and catalase (CAT) and malondialdehyde (MDA) levels in serum, egg yolk and egg white of laying hens. Ninety-six white Lohman laying hens aged of 24 weeks were randomly divided into 4 groups. The groups were fed with the diets that consisted of basal diet (2770 kcal/kg metabolic energy and 17 % crude protein) (Control), basal diet + 250 mg / kg Vit-E (Trial-1), basal diet + 0.9 mg/ kg Se (Trial-2) and basal diet + 250 mg / kg Vit-E + 0.9 mg/ kg Se (Trial-3) respectively for 12 weeks. It was found out that when the treatment and control groups were compared in terms of enzymes in serum and egg samples while SOD, CAT and GSH-Px activities increased (p<0.05) and the levels of MDA decreased in the samples of treatment groups (p<0.05). The results of the present study, is recommended that organic selenium and vitamin E supplemental of laying hen diets, alone or together, increased activites of antioxidan enzymes and decreased MDA concentrations in serum and egg.

Kaynakça

  • Ahmad H, Tian J, Wang J, Khan M A, Wang Y, Zhang L, Wang T. 2012. Effects of Dietary Sodium Selenite and Selenium Yeast on Antioxidant Enzyme Activities and Oxidative Stability of Chicken Breast Meat. Journal of Agricultural Food Chemistry 60: 7111−7120.
  • Beutler E. 1975. Red Cell Metabolism. in a Manual of Biochemical Methods. Grune Strottan, New York 67-69.
  • Boostani A, Sadeghi A, Mousavi S, Chamani M, Kashan N. 2015. Effects of Organic, İnorganic, and Nano-Se on Growth Performance, Antioxidant Capacity, Cellular and Humoral İmmune Responses İn Broiler Chickens Exposed To Oxidative Stress. Livestock Science, 178:330–33
  • Chen G, Wu J, Li C. 2014. Effect of Different Selenium Sources on Production Performance and Biochemical Parameters of Broilers. Journal of Animal Physiology and Animal Nutrition, 2014;98(4):747–54.
  • Czech A, Ognik K, Grela E R. 2012. Efficacy of a Mixture of Synthetic Antioxidant and Protein-Xanthophyll Alfalfa Concentrate In Turkey Hens Feeding. Archiv fur Geflugelkunde 76: 105-112.
  • Dalia AM, Loh TC, Sazili, AQ, Jahromi MF, Samsudin AA. 2017. The Effect of Dietary Bacterial Organic Selenium on Growth Performance, Antioxidant Capacity, and Selenoproteins Gene Expression İn Broiler Chickens. Dalia et al. BMC Veterinary Research. 13:254-265
  • Gajcevic Z, Kralik G, Has-Schon E, Pavic V. 2009. Effects of Organic Selenium Supplemented to Layer Diet on Table Egg Freshness and Selenium Content. Italian Journal of Animal Science, 8: 189-199.
  • Gaweł S, Wardas M, Niedworok E, Wardas P. 2004. Malondialdehyde (MDA) As a Lipid Peroxidation Marker.Wiadomosci lekarskie (Warsaw, Poland: 1960). 2004;57(9–10):453–5.
  • Goth L A. 1991. Simple Method for Determination of Serum Catalase Activity and Revision of Reference Range. Clinica Chimica Acta 196: 143-152.
  • Harsini HG, Habiviyon M, Moeini, MM, Abdolmohammad AR. 2012. Effects of Dietary Selenium, Vitamin E, and Their Combination on Growth, Serum Metabolites, and Antioxidant Defense System in Skeletal Muscle of Broilers Under Heat Stress. Trace Elements Research, 148: 322-330.
  • Huang B, Zhang J, Hou J, Chen C. 2003. Free Radical Scavenging Efficiency of Nano-Se in Vitro. Free Radical Biology and Medicine, 35: 805-813.
  • Jiang Z, Lin Y, Zhou G, Luo L, Jiang S, Chen F. 2009. Effects of Dietary Selenomethionine Supplementation on Growth Performance, Meat Quality and Antioxidant Property in Yellow Broilers. Journal of Agricultural and Food Chemistry, 57(20):9769–72.
  • Kaya H, Kaya A, Çelebi Ş, Macit M. 2013. Effects of Dietary Supplementation of Essential Oils and Vitamin E on Performance, Egg Quality and Escherichia Coli Count in Excreta.Indian Journal Of Animal Research, 47: 515-520
  • Khan RU. 2011. Antioxidants and Poultry Semen Quality. Worlds Poultry Science Journal 67: 297-308.
  • Leeson S, Namkung H, Caston L, Durosoy S, Schlegel P. 2008. Comparison of Selenium Levels and Sources and Dietary Fat Quality in Diets for Broiler Breeders and Layer Hens. Poultry Science 87: 2605-2612.
  • Leng LJ, Bobcek R, Kuricova S, Boldizarova K, Grešakova L, Ševickova Z, Revajova V, Levkutova M, Levkut M. 2003. Comparative Metabolic and İmmune Response of Chickens Diets Containing İnorganic Selenium and Sel- Plextm Organic Selenium. In: T.P. Nottingham Univer-sity Press, Nottingham, UK, pp 131-145pp. 131-145
  • Li KX, Wang JS, Yuan D, Zhao RX, Wang YX, Zhan XA. 2018. Effects of Different Selenium Sources and Levels on Antioxidant Status in Broiler Breeders. Asian Australasian Journal of Animal Sciences 31: 1939-1945
  • Mohapatra P, Swain RK, Mishra SK, Behera T, Swain P, Mishra SS, Behura NC, Sabat SC, Sethy K, Dhama K, Jayasankar P. 2014. Effects of Dietary Nano-Selenium on Tissue Selenium Deposition, Antioxidant Status and İmmune Functions in Layer Chicks. International Journal of Pharmacology, 10: 160-167
  • Nadia L, Radwan TA, Salah Eldin AA. EL-Zaiat and Mona AS, Mostafa A. 2015. Effect of Dietary Nano-Selenium Supplementation on Selenium Content and Oxidative Stability in Table Eggs and Productive Performance of Laying Hens. International Journal of Poultry Science, 14 (3): 161-176.
  • Ohkawa H, Ohishi N, Yagi K. 1979. Assay for Lipid Peroxides in Animal Tissues by Thiobarbituric Acid Reaction. Analytical Biochemistry, 95: 351-358.
  • Pan CL, Huang KH, Zhao YC, et al. 2008. Effects of Different Selenium Sources on Serum Selenium Content and Antioxidant Capacity of Laying Hens. Journal of Nanjing Agricultural University, 31:91-6.
  • Pappas AC, Zoidis E, Surai PF, Zervas G. 2008. Selenoproteins and Maternal Nutrition. Comparatıve Bıochemıstry and Physıology B-Bıochemıstry & Molecular Bıology. 151: 361- 372.
  • Payne R, Southern L. 2005. Comparison of İnorganic and Organic Selenium Sources for Broilers. Poultry Science, 84(6):898–902.
  • Placha I, Takacova J, Ryzner M, et a 2014. Effect of Thyme Essential Oil and Selenium on İntestine İntegrity and Antioxidant Status of Broilers. British Poultry Science, (55):105-14
  • Petrovič V, Boldižárová K, Faix, Š.2006. Antioxidant and Selenium Status of Laying Hens Fed with Diets Supplemented with Selenite or Se-Yeast. Journal of Animal and Feed Sciences, 15:435-44.
  • Rayman MP. 2004. The use of High-Selenium Yeast to Raise Selenium Status: How does it Measure up? British Journal of Nutrition, 92: 557-573.
  • Rengaraj D, Hong Y. 2015. Effects of Dietary Vitamin E on Fertility Functions in Poultry Species. International Journal of Molecular Sciences,16: 9910–9921.
  • Sayiner S, Karagul H. 2017. Blood Selenium and Vitamin E Levels in Heifers: Regional and Seasonal Differences in Northern Cyprus. Pakistan Journal of Zoology, 49: 669-676.
  • Soydan M, Utlu N. 2018. Effect of Addition of Organic Selenium and Vitamin E on Some Bioelement Levels in Blood and Egg Samples of Laying Hens. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, 13(2): 174-181.
  • Surai PF. 2000a. Organic Selenium: Benefits to Animals and Humans, a Biochemist’s View. In: T.P. Lyons And K.A Jacques Biotechnology İn The Feed İndustry. Nottingham University Press, Nottingham, UK, pp 205-260.
  • Surai PF. 2000b. Selenium in Poultry Nutrition. 2. Reproduction, Egg and Meat Quality and Practical Applications. World's Poultry Science Journal, 58:431-450.
  • Surai PF, Fisinin VI, Karadas F. 2016. Antioxidant Systems in Chick Embryo Development. Part 1. Vitamin E, Carotenoids and Selenium. Animal Nutrition 2: 1-11.
  • Ting S, Yeh HS, Lien TF. 2011. Effect of Supplemental Levels of Hesperetin and Naringenin on Egg Quality, Serum Traits and Antioxidant Activity of Laying Hens. Animal Feed Science and Technology,163: 59-66.
  • Wakebe M. 1999. Organic Selenium and Egg Freshness. Japanese Patent Office, Application Heisei 8-179629. Poster Presentation 15th Annual Symp. on Biotechnology in the Feed Industry,Lexington, KI, USA.
  • Wang, Z.G., X.J. Pan, W.Q. Zhang, Z.Q. Peng, R.Q. Zhao and G.H. Zhou, 2010. Methionine and Selenium Yeast Supplementation of the Maternal Diets Affects Antioxidant Activity of Breeding Eggs. Poultry Science, 89:31-937.
  • Yasin M, Asghar A, Anjum FM, Butt MS, Khan MI, Arshad MS, Shahid M, El-Ghorab AH, Shibamoto T. 2012. Oxidative Stability Enhancement of Broiler Bird Meats With Α-Lipoic Acid and Α-Tocopherol Acetate Supplemented Feed. Food Chemistry, 131: 768−773.
  • Zhang JS, Wang XF, Xu TW. 2008. Elemental Selenium at Nano Size (Nano-Se) As a Potential Chemopreventive Agent with Reduced Risk of Selenium Toxicity: Comparison with Semethylselenocysteine in Mice. Toxicological Sciences , 101:22-31.
  • Zhang L, Wang Y, Zhou Y, Zheng L, Zhan X, Pu Q. 2014. Different Sources of Maternal Selenium Affect Selenium Retention, Antioxidant Status, and Meat Quality of 56-Day-Old Offspring of Broiler Breeders. Poultry Science, 93(9):2210–9.
  • Zduńczyk Z, Drażbo A, Jankowski J, Juśkiewicz J, Czech A, Antoszkiewicz Z. 2013. The Effect of Different Dietary Levels of Vitamin E and Selenium on Antioxidant Status and Immunological Markers in Serum of Laying Hens. Polish Journal of Veterinary Sciences, 2: 333–339.
  • Zhou X, Wang Y. 2011. Influence of Dietary Nano Elemental Selenium on Growth Performance, Tissue Selenium Distribution, Meat Quality and Glutathione Peroxidase Activity in Guangxi Yellow Chicken. Poultry Science, 90: 680-686.

Influence of Vitamin E and Organic Selenium Supplementation on Antioxidant Enzymes Activities in Blood and Egg Samples of Laying Hens

Yıl 2020, Cilt: 10 Sayı: 1, 694 - 701, 01.03.2020
https://doi.org/10.21597/jist.544969

Öz

This experiment was conducted to evaluate the effects of dietary vitamin E (α-tocopherol acetate) and selenium (selenomethionine) and a combination of the two, on the activities of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and catalase (CAT) and malondialdehyde (MDA) levels in serum, egg yolk and egg white of laying hens. Ninety-six white Lohman laying hens aged of 24 weeks were randomly divided into 4 groups. The groups were fed with the diets that consisted of basal diet (2770 kcal/kg metabolic energy and 17 % crude protein) (Control), basal diet + 250 mg / kg Vit-E (Trial-1), basal diet + 0.9 mg/ kg Se (Trial-2) and basal diet + 250 mg / kg Vit-E + 0.9 mg/ kg Se (Trial-3) respectively for 12 weeks. It was found out that when the treatment and control groups were compared in terms of enzymes in serum and egg samples while SOD, CAT and GSH-Px activities increased (p<0.05) and the levels of MDA decreased in the samples of treatment groups (p<0.05). The results of the present study, is recommended that organic selenium and vitamin E supplemental of laying hen diets, alone or together, increased activites of antioxidan enzymes and decreased MDA concentrations in serum and egg.

Kaynakça

  • Ahmad H, Tian J, Wang J, Khan M A, Wang Y, Zhang L, Wang T. 2012. Effects of Dietary Sodium Selenite and Selenium Yeast on Antioxidant Enzyme Activities and Oxidative Stability of Chicken Breast Meat. Journal of Agricultural Food Chemistry 60: 7111−7120.
  • Beutler E. 1975. Red Cell Metabolism. in a Manual of Biochemical Methods. Grune Strottan, New York 67-69.
  • Boostani A, Sadeghi A, Mousavi S, Chamani M, Kashan N. 2015. Effects of Organic, İnorganic, and Nano-Se on Growth Performance, Antioxidant Capacity, Cellular and Humoral İmmune Responses İn Broiler Chickens Exposed To Oxidative Stress. Livestock Science, 178:330–33
  • Chen G, Wu J, Li C. 2014. Effect of Different Selenium Sources on Production Performance and Biochemical Parameters of Broilers. Journal of Animal Physiology and Animal Nutrition, 2014;98(4):747–54.
  • Czech A, Ognik K, Grela E R. 2012. Efficacy of a Mixture of Synthetic Antioxidant and Protein-Xanthophyll Alfalfa Concentrate In Turkey Hens Feeding. Archiv fur Geflugelkunde 76: 105-112.
  • Dalia AM, Loh TC, Sazili, AQ, Jahromi MF, Samsudin AA. 2017. The Effect of Dietary Bacterial Organic Selenium on Growth Performance, Antioxidant Capacity, and Selenoproteins Gene Expression İn Broiler Chickens. Dalia et al. BMC Veterinary Research. 13:254-265
  • Gajcevic Z, Kralik G, Has-Schon E, Pavic V. 2009. Effects of Organic Selenium Supplemented to Layer Diet on Table Egg Freshness and Selenium Content. Italian Journal of Animal Science, 8: 189-199.
  • Gaweł S, Wardas M, Niedworok E, Wardas P. 2004. Malondialdehyde (MDA) As a Lipid Peroxidation Marker.Wiadomosci lekarskie (Warsaw, Poland: 1960). 2004;57(9–10):453–5.
  • Goth L A. 1991. Simple Method for Determination of Serum Catalase Activity and Revision of Reference Range. Clinica Chimica Acta 196: 143-152.
  • Harsini HG, Habiviyon M, Moeini, MM, Abdolmohammad AR. 2012. Effects of Dietary Selenium, Vitamin E, and Their Combination on Growth, Serum Metabolites, and Antioxidant Defense System in Skeletal Muscle of Broilers Under Heat Stress. Trace Elements Research, 148: 322-330.
  • Huang B, Zhang J, Hou J, Chen C. 2003. Free Radical Scavenging Efficiency of Nano-Se in Vitro. Free Radical Biology and Medicine, 35: 805-813.
  • Jiang Z, Lin Y, Zhou G, Luo L, Jiang S, Chen F. 2009. Effects of Dietary Selenomethionine Supplementation on Growth Performance, Meat Quality and Antioxidant Property in Yellow Broilers. Journal of Agricultural and Food Chemistry, 57(20):9769–72.
  • Kaya H, Kaya A, Çelebi Ş, Macit M. 2013. Effects of Dietary Supplementation of Essential Oils and Vitamin E on Performance, Egg Quality and Escherichia Coli Count in Excreta.Indian Journal Of Animal Research, 47: 515-520
  • Khan RU. 2011. Antioxidants and Poultry Semen Quality. Worlds Poultry Science Journal 67: 297-308.
  • Leeson S, Namkung H, Caston L, Durosoy S, Schlegel P. 2008. Comparison of Selenium Levels and Sources and Dietary Fat Quality in Diets for Broiler Breeders and Layer Hens. Poultry Science 87: 2605-2612.
  • Leng LJ, Bobcek R, Kuricova S, Boldizarova K, Grešakova L, Ševickova Z, Revajova V, Levkutova M, Levkut M. 2003. Comparative Metabolic and İmmune Response of Chickens Diets Containing İnorganic Selenium and Sel- Plextm Organic Selenium. In: T.P. Nottingham Univer-sity Press, Nottingham, UK, pp 131-145pp. 131-145
  • Li KX, Wang JS, Yuan D, Zhao RX, Wang YX, Zhan XA. 2018. Effects of Different Selenium Sources and Levels on Antioxidant Status in Broiler Breeders. Asian Australasian Journal of Animal Sciences 31: 1939-1945
  • Mohapatra P, Swain RK, Mishra SK, Behera T, Swain P, Mishra SS, Behura NC, Sabat SC, Sethy K, Dhama K, Jayasankar P. 2014. Effects of Dietary Nano-Selenium on Tissue Selenium Deposition, Antioxidant Status and İmmune Functions in Layer Chicks. International Journal of Pharmacology, 10: 160-167
  • Nadia L, Radwan TA, Salah Eldin AA. EL-Zaiat and Mona AS, Mostafa A. 2015. Effect of Dietary Nano-Selenium Supplementation on Selenium Content and Oxidative Stability in Table Eggs and Productive Performance of Laying Hens. International Journal of Poultry Science, 14 (3): 161-176.
  • Ohkawa H, Ohishi N, Yagi K. 1979. Assay for Lipid Peroxides in Animal Tissues by Thiobarbituric Acid Reaction. Analytical Biochemistry, 95: 351-358.
  • Pan CL, Huang KH, Zhao YC, et al. 2008. Effects of Different Selenium Sources on Serum Selenium Content and Antioxidant Capacity of Laying Hens. Journal of Nanjing Agricultural University, 31:91-6.
  • Pappas AC, Zoidis E, Surai PF, Zervas G. 2008. Selenoproteins and Maternal Nutrition. Comparatıve Bıochemıstry and Physıology B-Bıochemıstry & Molecular Bıology. 151: 361- 372.
  • Payne R, Southern L. 2005. Comparison of İnorganic and Organic Selenium Sources for Broilers. Poultry Science, 84(6):898–902.
  • Placha I, Takacova J, Ryzner M, et a 2014. Effect of Thyme Essential Oil and Selenium on İntestine İntegrity and Antioxidant Status of Broilers. British Poultry Science, (55):105-14
  • Petrovič V, Boldižárová K, Faix, Š.2006. Antioxidant and Selenium Status of Laying Hens Fed with Diets Supplemented with Selenite or Se-Yeast. Journal of Animal and Feed Sciences, 15:435-44.
  • Rayman MP. 2004. The use of High-Selenium Yeast to Raise Selenium Status: How does it Measure up? British Journal of Nutrition, 92: 557-573.
  • Rengaraj D, Hong Y. 2015. Effects of Dietary Vitamin E on Fertility Functions in Poultry Species. International Journal of Molecular Sciences,16: 9910–9921.
  • Sayiner S, Karagul H. 2017. Blood Selenium and Vitamin E Levels in Heifers: Regional and Seasonal Differences in Northern Cyprus. Pakistan Journal of Zoology, 49: 669-676.
  • Soydan M, Utlu N. 2018. Effect of Addition of Organic Selenium and Vitamin E on Some Bioelement Levels in Blood and Egg Samples of Laying Hens. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, 13(2): 174-181.
  • Surai PF. 2000a. Organic Selenium: Benefits to Animals and Humans, a Biochemist’s View. In: T.P. Lyons And K.A Jacques Biotechnology İn The Feed İndustry. Nottingham University Press, Nottingham, UK, pp 205-260.
  • Surai PF. 2000b. Selenium in Poultry Nutrition. 2. Reproduction, Egg and Meat Quality and Practical Applications. World's Poultry Science Journal, 58:431-450.
  • Surai PF, Fisinin VI, Karadas F. 2016. Antioxidant Systems in Chick Embryo Development. Part 1. Vitamin E, Carotenoids and Selenium. Animal Nutrition 2: 1-11.
  • Ting S, Yeh HS, Lien TF. 2011. Effect of Supplemental Levels of Hesperetin and Naringenin on Egg Quality, Serum Traits and Antioxidant Activity of Laying Hens. Animal Feed Science and Technology,163: 59-66.
  • Wakebe M. 1999. Organic Selenium and Egg Freshness. Japanese Patent Office, Application Heisei 8-179629. Poster Presentation 15th Annual Symp. on Biotechnology in the Feed Industry,Lexington, KI, USA.
  • Wang, Z.G., X.J. Pan, W.Q. Zhang, Z.Q. Peng, R.Q. Zhao and G.H. Zhou, 2010. Methionine and Selenium Yeast Supplementation of the Maternal Diets Affects Antioxidant Activity of Breeding Eggs. Poultry Science, 89:31-937.
  • Yasin M, Asghar A, Anjum FM, Butt MS, Khan MI, Arshad MS, Shahid M, El-Ghorab AH, Shibamoto T. 2012. Oxidative Stability Enhancement of Broiler Bird Meats With Α-Lipoic Acid and Α-Tocopherol Acetate Supplemented Feed. Food Chemistry, 131: 768−773.
  • Zhang JS, Wang XF, Xu TW. 2008. Elemental Selenium at Nano Size (Nano-Se) As a Potential Chemopreventive Agent with Reduced Risk of Selenium Toxicity: Comparison with Semethylselenocysteine in Mice. Toxicological Sciences , 101:22-31.
  • Zhang L, Wang Y, Zhou Y, Zheng L, Zhan X, Pu Q. 2014. Different Sources of Maternal Selenium Affect Selenium Retention, Antioxidant Status, and Meat Quality of 56-Day-Old Offspring of Broiler Breeders. Poultry Science, 93(9):2210–9.
  • Zduńczyk Z, Drażbo A, Jankowski J, Juśkiewicz J, Czech A, Antoszkiewicz Z. 2013. The Effect of Different Dietary Levels of Vitamin E and Selenium on Antioxidant Status and Immunological Markers in Serum of Laying Hens. Polish Journal of Veterinary Sciences, 2: 333–339.
  • Zhou X, Wang Y. 2011. Influence of Dietary Nano Elemental Selenium on Growth Performance, Tissue Selenium Distribution, Meat Quality and Glutathione Peroxidase Activity in Guangxi Yellow Chicken. Poultry Science, 90: 680-686.
Toplam 40 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Hayvansal Üretim (Diğer)
Bölüm Zootekni / Animal Science
Yazarlar

Cihangir Timur Bu kişi benim 0000-0002-0606-9362

Necati Utlu 0000-0001-6354-6922

Yayımlanma Tarihi 1 Mart 2020
Gönderilme Tarihi 26 Mart 2019
Kabul Tarihi 23 Ekim 2019
Yayımlandığı Sayı Yıl 2020 Cilt: 10 Sayı: 1

Kaynak Göster

APA Timur, C., & Utlu, N. (2020). Influence of Vitamin E and Organic Selenium Supplementation on Antioxidant Enzymes Activities in Blood and Egg Samples of Laying Hens. Journal of the Institute of Science and Technology, 10(1), 694-701. https://doi.org/10.21597/jist.544969
AMA Timur C, Utlu N. Influence of Vitamin E and Organic Selenium Supplementation on Antioxidant Enzymes Activities in Blood and Egg Samples of Laying Hens. Iğdır Üniv. Fen Bil Enst. Der. Mart 2020;10(1):694-701. doi:10.21597/jist.544969
Chicago Timur, Cihangir, ve Necati Utlu. “Influence of Vitamin E and Organic Selenium Supplementation on Antioxidant Enzymes Activities in Blood and Egg Samples of Laying Hens”. Journal of the Institute of Science and Technology 10, sy. 1 (Mart 2020): 694-701. https://doi.org/10.21597/jist.544969.
EndNote Timur C, Utlu N (01 Mart 2020) Influence of Vitamin E and Organic Selenium Supplementation on Antioxidant Enzymes Activities in Blood and Egg Samples of Laying Hens. Journal of the Institute of Science and Technology 10 1 694–701.
IEEE C. Timur ve N. Utlu, “Influence of Vitamin E and Organic Selenium Supplementation on Antioxidant Enzymes Activities in Blood and Egg Samples of Laying Hens”, Iğdır Üniv. Fen Bil Enst. Der., c. 10, sy. 1, ss. 694–701, 2020, doi: 10.21597/jist.544969.
ISNAD Timur, Cihangir - Utlu, Necati. “Influence of Vitamin E and Organic Selenium Supplementation on Antioxidant Enzymes Activities in Blood and Egg Samples of Laying Hens”. Journal of the Institute of Science and Technology 10/1 (Mart 2020), 694-701. https://doi.org/10.21597/jist.544969.
JAMA Timur C, Utlu N. Influence of Vitamin E and Organic Selenium Supplementation on Antioxidant Enzymes Activities in Blood and Egg Samples of Laying Hens. Iğdır Üniv. Fen Bil Enst. Der. 2020;10:694–701.
MLA Timur, Cihangir ve Necati Utlu. “Influence of Vitamin E and Organic Selenium Supplementation on Antioxidant Enzymes Activities in Blood and Egg Samples of Laying Hens”. Journal of the Institute of Science and Technology, c. 10, sy. 1, 2020, ss. 694-01, doi:10.21597/jist.544969.
Vancouver Timur C, Utlu N. Influence of Vitamin E and Organic Selenium Supplementation on Antioxidant Enzymes Activities in Blood and Egg Samples of Laying Hens. Iğdır Üniv. Fen Bil Enst. Der. 2020;10(1):694-701.