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Usage of Lactobacillus rhamnosus as a Probiotic in Sea Bass (Dicentrarchus labrax)

Yıl 2020, Cilt: 5 Sayı: 1, 93 - 99, 31.03.2020

Fatma Öztürk Ömer Memduh Esendal

https://doi.org/10.35229/jaes.687057

Öz

Bu çalışmada, levrek balıklarında (Dicentrarchus labrax) probiyotik olarak kullanılan Lactobacillus rhamnosus (ATCC 53103)’un deformasyon oranı, yaşama oranı, ağırlık artışı ve bağırsak mikroflorası üzerine etkisi araştırılmıştır. Bu amaçla, probiyotik etken son konsantrasyonu yetiştirme suyunda 106 kob/ml ve Artemia nauplii kültüründe ise 108 kob/ml olacak şekilde ilave edilmiştir. Bu şekilde probiyotik ilavesi 50. güne kadar devam etmiştir. Bu sürenin sonunda her iki grubun toz yemine 109 kob/g düzeyinde probiyotik ilavesi yapılmıştır. Deneme sonunda larvalardaki deformasyon oranları; yetiştirme suyuna probiyotik ilave edilen grupta %5, Artemia kültürüne ilave edilen grupta %2 ve kontrol grubunda %7 olarak tespit edilmiştir (P<0.01). Deneme gruplarının yaşama oranı kontrol grubuna kıyasla önemli düzeyde yüksek bulunmuştur (P<0.01). Probiyotik ilavesinin balıkların ağırlık artışları üzerinde önemli bir fark oluşturmadığı tespit edilmiştir (P>0.01). Ayrıca, Artemia kültürüne probiyotik ilavesinin Vibrio spp. sayısında önemli düzeyde azalmaya neden olduğu belirlenmiştir (P<0.01).

Anahtar Kelimeler

Deformation Lactobacillus rhamnosus microflora sea bass

Destekleyen Kurum

TUBİTAK

Proje Numarası

Project No: TOVAG 104-O-496

Kaynakça

  • Aubin, J., Gatesoupe, F.J., Labbé, L. & Lebrun, L. (2005). Trial of probiotics to prevent the vertebral column compression syndrome in rainbow trout (Oncorhynchus mykiss Walbaum). Aquaculture Research, 36(8), 758-767.
  • Byun, J.W., Park, S. & Benno, Y. (1997). Probiotic effect of Lactobacillus sp. DS–12 in flounder (Paralichthys olivaceus). Journal of General and Applied Microbiology, 43, 305-308.
  • Carnevali, O., de Vivo, L., Sulpizio, R., Gioacchini, G., Olivotto, I., Silvi, S. & Cresci, A. (2006). Growth improvement by probiotic in European sea bass juveniles (Dicentrarchus labrax, L.), with particular attention to IGF-1, myostatin and cortisol gene expression. Aquaculture, 258(1-4), 430-438.
  • Franke, A., Roth, O., De Schryver, P., Bayer, T., Garcia-Gonzalez, L., Künzel, S. & Clemmesen, C. (2017). Poly-β-hydroxybutyrate administration during early life: effects on performance, immunity and microbial community of European sea bass yolk-sac larvae. Scientific Reports, 7(1), 15022. Doi: 0.1038/s41598-017-14785-z
  • Gatesoupe, F.J. (1991). The effect of three strains of lactic bacteria on the production rate of rotifers, Brachionus plicatilis, and their dietary value for larval turbot, Scophthalmus maximus. Aquaculture, 96(3-4), 335-342.
  • Gil‐Martens, L. (2010). Inflammation as a potential risk factor for spinal deformities in farmed Atlantic salmon (Salmo salar L.). Journal of Applied Ichthyology, 26(2), 350-354.
  • Gobi, N., Vaseeharan, B., Chen, J.C., Rekha, R., Vijayakumar, S., Anjugam, M. & Iswarya, A. (2018). Dietary supplementation of probiotic Bacillus licheniformis Dahb1 improves growth performance, mucus and serum immune parameters, antioxidant enzyme activity as well as resistance against Aeromonas hydrophila in tilapia Oreochromis mossambicus. Fish & Shellfish Immunology, 74, 501-508. Doi: 10.1016/j.fsi.2017.12.066
  • Goda, A.M., Omar, E.A., Srour, T.M., Kotiet, A.M., El-Haroun, E. & Davies, S..J. (2018). Effect of diets supplemented with feed additives on growth, feed utilization, survival, body composition and intestinal bacterial load of early weaning European seabass, Dicentrarchus labrax post-larvae. Aquaculture International, 26(1), 169-183. Doi: 10.1007/s10499-017-0200-8d
  • Hameed, A.S. & Balasubramanian, G. (2000). Antibiotic resistance in bacteria isolated from Artemia nauplii and efficacy of formaldehyde to control bacterial load. Aquaculture, 183(3-4), 195-205.
  • Hamza, A., Fdhila, K., Zouiten, D. & Masmoudi, A.S. (2016). Virgibacillus proomii and Bacillus mojavensis as probiotics in sea bass (Dicentrarchus labrax) larvae: effects on growth performance and digestive enzyme activities. Fish Physiology and Biochemistry, 42(2), 495-507. Doi: 10.1007/s10695-015-0154-6
  • Harrigan, W.F (1998). Laboratory Methods in Food Microbiology. 3rd ed. San Diego, CA, USA: Academic Press.
  • Harzevili, A.R., Duffel, H.V., Dhert, P., Swings, J., and Sargeloos, P. (1998). Use of potential probiotic Lactococcus lactis AR21 strain for the enhancement of growth in the rotifer Brachionus plicatilis. Aquaculture Research, 29, 411-417. Doi: 0.1046/j.1365-2109.1998.00217.x
  • Jöborn, A., Olsson, J.C., Westerdahl, A., Conway, P.L. & Kjelleberg, S. (1997). Colonization in the fish intestinal tract and production of inhibitory substances in intestinal mucus and faecal extracts by Carnobacterium sp. strain K1. Journal of Fish Disease, 20, 383-392. Doi: 10.1046/j.1365-2761.1997.00316.x
  • Lamari, F., Castex, M., Larcher, T., Ledevin, M., Mazurais, D., Bakhrouf, A. & Gatesoupe, F.J. (2013). Comparison of the effects of the dietary addition of two lactic acid bacteria on the development and conformation of sea bass larvae, Dicentrarchus labrax, and the influence on associated microbiota. Aquaculture, 376, 137-145. Doi: 10.1016/j.aquaculture.2012.11.016
  • Lin, H.L., Shiu, Y.L., Chiu, C.S., Huang, S.L. & Liu, C.H. (2017). Screening probiotic candidates for a mixture of probiotics to enhance the growth performance, immunity, and disease resistance of Asian seabass, Lates calcarifer (Bloch), against Aeromonas hydrophila. Fish & Shellfish Immunology, 60, 474-482. Doi: 10.1016/j.fsi.2016.11.026.
  • Ljubobratovic, U., Kosanovic, D., Vukotic, G., Molnar, Z., Stanisavljevi, N.S., Ristovic, T. & Jeney, G. (2017). Supplementation of lactobacilli improves growth, regulates microbiota composition and suppresses skeletal anomalies in juvenile pike-perch (Sander lucioperca) reared in recirculating aquaculture system (RAS): a pilot study. Research in Veterinary Science, 115, 451-462. Doi: 10.1016/j.rvsc.2017.07.018
  • Muroga, K., Higashi, M. & Keitoku, H. (1987). The isolation of intestinal microflora of farmed red sea bream (Pagrus major) and black sea bream (Acanthopagrus schlegeli) at larval and juvenile stages. Aquaculture, 65, 79-88.
  • Nguyen, T.L., Park, C.I. & Kim, D.H. (2017). Improved growth rate and disease resistance in olive flounder, Paralichthys olivaceus, by probiotic Lactococcus lactis WFLU12 isolated from wild marine fish. Aquaculture, 471, 113-120. Doi: 10.1016/j.aquaculture.2017.01.008
  • Nikoskelainen, S., Ouwehand, A.C., Salminen, S. & Bylund, G. (2001). Protection of rainbow trout (Oncorhynchus mykiss) from furunculosis by Lactobacillus rhamnosus. Aquaculture, 198, 229-236.
  • Olsen, A.I., Olsen, Y., Attramadal, M.Y., Chiristie, K., Birkbeck, T.H., Skjermo, J. & Vadstein, O. (2000). Effect of short term feeding of microalgae on the bacterial flora associated with juvenile Artemia franciscana. Aquaculture, 190, 11-25.
  • Piccolo, G., Bovera, F., Lombardi, P., Mastellone, V., Nizza, S., Meo, C.D. & Marono, S. (2015). Effect of Lactobacillus plantarum on growth performance and hematological traits of European sea bass (Dicentrarchus labrax). Aquaculture International, 23(4), 1025-1032. Doi: 10.1007/s10499-014-9861-8
  • Reitan, K.I., Rainuzzo, J.R. & Olsen, Y. (1993). Nutritional effects of algal addition in first feeding tanks of turbot (S. maximus) larvae. Aquaculture, 118, 257-275.
  • Rollo, A., Sulpizio, R., Nardi, M., Silvi, S., Orpianesi, C., Caggiano, M. & Carnevali, O. (2006). Live microbial feed supplement in aquaculture for improvement of stress tolerance. Fish Physiology and Biochemistry, 32(2), 167-177. Doi: 10.1007/s10695-006-0009-2
  • Silvi, S., Nardi, M., Sulpizio, R., Orpianesi, C., Caggiano, M., Carnevali, O. & Cresci, A. (2008). Effect of the addition of Lactobacillus delbrueckii subsp. delbrueckii on the gut microbiota composition and contribution to the well-being of European sea bass. Microbiol Ecology in Health and Disease, 20(1), 53-59. Doi: 10.1080/08910600701876020
  • Sutthi, N., Thaimuangphol, W., Rodmongkoldee, M., Leelapatra, W. & Panase, P. (2018). Growth performances, survival rate, and biochemical parameters of Nile tilapia (Oreochromis niloticus) reared in water treated with probiotic. Comparative Clinical Pathology, 27(3), 597-603. Doi: 10.1007/s00580-017-2633-x
  • Tovar-Ramirez, D., Infante, Z.J., Cahu, C., Gatesoupe, F.J., Vazquez-Juarez, R. (2004). Influence of dietary live yeast on European sea bass larval development. Aquaculture, 234, 415-427. Doi:10.1016/j.aquaculture.2004.01.028
  • Van Doan, H., Hoseinifar, S. H., Khanongnuch, C., Kanpiengjai, A., Unban, K. & Srichaiyo, S. (2018). Host-associated probiotics boosted mucosal and serum immunity, disease resistance and growth performance of Nile tilapia (Oreochromis niloticus). Aquaculture, 491, 94-100. Doi: 10.1016/j.aquaculture.2018.03.019
  • Varsamos, S., Connes, R., Diaz, J. P., Barnabé, G. & Charmantier, G. (2001). Ontogeny of osmoregulation in the European sea bass Dicentrarchus labrax L. Marine Biology, 138(5), 909-915.
  • Vendrell, D., Balcázar, J.L., de Blas, I., Ruiz-Zarzuela, I., Gironés, O. & Múzquiz, J.L. ( 2008). Protection of rainbow trout from lactococcosis by probiotic bacteria. Comparative Immunology, Microbiology & Infectious Diseases, 31, 337-345. Doi:10.1016/j.cimid.2007.04.002
  • Villamil, L, Figueras, A., Planas, M. & Novoa, B. (2010). Pediococcus acidilactici in the culture of turbot larvae: administration pathways. Aquaculture, 307(1-2), 83-88. Doi:10.1016/j.aquaculture.2010.07.004

Yıl 2020, Cilt: 5 Sayı: 1, 93 - 99, 31.03.2020

Fatma Öztürk Ömer Memduh Esendal

https://doi.org/10.35229/jaes.687057

Öz

Proje Numarası

Project No: TOVAG 104-O-496

Kaynakça

  • Aubin, J., Gatesoupe, F.J., Labbé, L. & Lebrun, L. (2005). Trial of probiotics to prevent the vertebral column compression syndrome in rainbow trout (Oncorhynchus mykiss Walbaum). Aquaculture Research, 36(8), 758-767.
  • Byun, J.W., Park, S. & Benno, Y. (1997). Probiotic effect of Lactobacillus sp. DS–12 in flounder (Paralichthys olivaceus). Journal of General and Applied Microbiology, 43, 305-308.
  • Carnevali, O., de Vivo, L., Sulpizio, R., Gioacchini, G., Olivotto, I., Silvi, S. & Cresci, A. (2006). Growth improvement by probiotic in European sea bass juveniles (Dicentrarchus labrax, L.), with particular attention to IGF-1, myostatin and cortisol gene expression. Aquaculture, 258(1-4), 430-438.
  • Franke, A., Roth, O., De Schryver, P., Bayer, T., Garcia-Gonzalez, L., Künzel, S. & Clemmesen, C. (2017). Poly-β-hydroxybutyrate administration during early life: effects on performance, immunity and microbial community of European sea bass yolk-sac larvae. Scientific Reports, 7(1), 15022. Doi: 0.1038/s41598-017-14785-z
  • Gatesoupe, F.J. (1991). The effect of three strains of lactic bacteria on the production rate of rotifers, Brachionus plicatilis, and their dietary value for larval turbot, Scophthalmus maximus. Aquaculture, 96(3-4), 335-342.
  • Gil‐Martens, L. (2010). Inflammation as a potential risk factor for spinal deformities in farmed Atlantic salmon (Salmo salar L.). Journal of Applied Ichthyology, 26(2), 350-354.
  • Gobi, N., Vaseeharan, B., Chen, J.C., Rekha, R., Vijayakumar, S., Anjugam, M. & Iswarya, A. (2018). Dietary supplementation of probiotic Bacillus licheniformis Dahb1 improves growth performance, mucus and serum immune parameters, antioxidant enzyme activity as well as resistance against Aeromonas hydrophila in tilapia Oreochromis mossambicus. Fish & Shellfish Immunology, 74, 501-508. Doi: 10.1016/j.fsi.2017.12.066
  • Goda, A.M., Omar, E.A., Srour, T.M., Kotiet, A.M., El-Haroun, E. & Davies, S..J. (2018). Effect of diets supplemented with feed additives on growth, feed utilization, survival, body composition and intestinal bacterial load of early weaning European seabass, Dicentrarchus labrax post-larvae. Aquaculture International, 26(1), 169-183. Doi: 10.1007/s10499-017-0200-8d
  • Hameed, A.S. & Balasubramanian, G. (2000). Antibiotic resistance in bacteria isolated from Artemia nauplii and efficacy of formaldehyde to control bacterial load. Aquaculture, 183(3-4), 195-205.
  • Hamza, A., Fdhila, K., Zouiten, D. & Masmoudi, A.S. (2016). Virgibacillus proomii and Bacillus mojavensis as probiotics in sea bass (Dicentrarchus labrax) larvae: effects on growth performance and digestive enzyme activities. Fish Physiology and Biochemistry, 42(2), 495-507. Doi: 10.1007/s10695-015-0154-6
  • Harrigan, W.F (1998). Laboratory Methods in Food Microbiology. 3rd ed. San Diego, CA, USA: Academic Press.
  • Harzevili, A.R., Duffel, H.V., Dhert, P., Swings, J., and Sargeloos, P. (1998). Use of potential probiotic Lactococcus lactis AR21 strain for the enhancement of growth in the rotifer Brachionus plicatilis. Aquaculture Research, 29, 411-417. Doi: 0.1046/j.1365-2109.1998.00217.x
  • Jöborn, A., Olsson, J.C., Westerdahl, A., Conway, P.L. & Kjelleberg, S. (1997). Colonization in the fish intestinal tract and production of inhibitory substances in intestinal mucus and faecal extracts by Carnobacterium sp. strain K1. Journal of Fish Disease, 20, 383-392. Doi: 10.1046/j.1365-2761.1997.00316.x
  • Lamari, F., Castex, M., Larcher, T., Ledevin, M., Mazurais, D., Bakhrouf, A. & Gatesoupe, F.J. (2013). Comparison of the effects of the dietary addition of two lactic acid bacteria on the development and conformation of sea bass larvae, Dicentrarchus labrax, and the influence on associated microbiota. Aquaculture, 376, 137-145. Doi: 10.1016/j.aquaculture.2012.11.016
  • Lin, H.L., Shiu, Y.L., Chiu, C.S., Huang, S.L. & Liu, C.H. (2017). Screening probiotic candidates for a mixture of probiotics to enhance the growth performance, immunity, and disease resistance of Asian seabass, Lates calcarifer (Bloch), against Aeromonas hydrophila. Fish & Shellfish Immunology, 60, 474-482. Doi: 10.1016/j.fsi.2016.11.026.
  • Ljubobratovic, U., Kosanovic, D., Vukotic, G., Molnar, Z., Stanisavljevi, N.S., Ristovic, T. & Jeney, G. (2017). Supplementation of lactobacilli improves growth, regulates microbiota composition and suppresses skeletal anomalies in juvenile pike-perch (Sander lucioperca) reared in recirculating aquaculture system (RAS): a pilot study. Research in Veterinary Science, 115, 451-462. Doi: 10.1016/j.rvsc.2017.07.018
  • Muroga, K., Higashi, M. & Keitoku, H. (1987). The isolation of intestinal microflora of farmed red sea bream (Pagrus major) and black sea bream (Acanthopagrus schlegeli) at larval and juvenile stages. Aquaculture, 65, 79-88.
  • Nguyen, T.L., Park, C.I. & Kim, D.H. (2017). Improved growth rate and disease resistance in olive flounder, Paralichthys olivaceus, by probiotic Lactococcus lactis WFLU12 isolated from wild marine fish. Aquaculture, 471, 113-120. Doi: 10.1016/j.aquaculture.2017.01.008
  • Nikoskelainen, S., Ouwehand, A.C., Salminen, S. & Bylund, G. (2001). Protection of rainbow trout (Oncorhynchus mykiss) from furunculosis by Lactobacillus rhamnosus. Aquaculture, 198, 229-236.
  • Olsen, A.I., Olsen, Y., Attramadal, M.Y., Chiristie, K., Birkbeck, T.H., Skjermo, J. & Vadstein, O. (2000). Effect of short term feeding of microalgae on the bacterial flora associated with juvenile Artemia franciscana. Aquaculture, 190, 11-25.
  • Piccolo, G., Bovera, F., Lombardi, P., Mastellone, V., Nizza, S., Meo, C.D. & Marono, S. (2015). Effect of Lactobacillus plantarum on growth performance and hematological traits of European sea bass (Dicentrarchus labrax). Aquaculture International, 23(4), 1025-1032. Doi: 10.1007/s10499-014-9861-8
  • Reitan, K.I., Rainuzzo, J.R. & Olsen, Y. (1993). Nutritional effects of algal addition in first feeding tanks of turbot (S. maximus) larvae. Aquaculture, 118, 257-275.
  • Rollo, A., Sulpizio, R., Nardi, M., Silvi, S., Orpianesi, C., Caggiano, M. & Carnevali, O. (2006). Live microbial feed supplement in aquaculture for improvement of stress tolerance. Fish Physiology and Biochemistry, 32(2), 167-177. Doi: 10.1007/s10695-006-0009-2
  • Silvi, S., Nardi, M., Sulpizio, R., Orpianesi, C., Caggiano, M., Carnevali, O. & Cresci, A. (2008). Effect of the addition of Lactobacillus delbrueckii subsp. delbrueckii on the gut microbiota composition and contribution to the well-being of European sea bass. Microbiol Ecology in Health and Disease, 20(1), 53-59. Doi: 10.1080/08910600701876020
  • Sutthi, N., Thaimuangphol, W., Rodmongkoldee, M., Leelapatra, W. & Panase, P. (2018). Growth performances, survival rate, and biochemical parameters of Nile tilapia (Oreochromis niloticus) reared in water treated with probiotic. Comparative Clinical Pathology, 27(3), 597-603. Doi: 10.1007/s00580-017-2633-x
  • Tovar-Ramirez, D., Infante, Z.J., Cahu, C., Gatesoupe, F.J., Vazquez-Juarez, R. (2004). Influence of dietary live yeast on European sea bass larval development. Aquaculture, 234, 415-427. Doi:10.1016/j.aquaculture.2004.01.028
  • Van Doan, H., Hoseinifar, S. H., Khanongnuch, C., Kanpiengjai, A., Unban, K. & Srichaiyo, S. (2018). Host-associated probiotics boosted mucosal and serum immunity, disease resistance and growth performance of Nile tilapia (Oreochromis niloticus). Aquaculture, 491, 94-100. Doi: 10.1016/j.aquaculture.2018.03.019
  • Varsamos, S., Connes, R., Diaz, J. P., Barnabé, G. & Charmantier, G. (2001). Ontogeny of osmoregulation in the European sea bass Dicentrarchus labrax L. Marine Biology, 138(5), 909-915.
  • Vendrell, D., Balcázar, J.L., de Blas, I., Ruiz-Zarzuela, I., Gironés, O. & Múzquiz, J.L. ( 2008). Protection of rainbow trout from lactococcosis by probiotic bacteria. Comparative Immunology, Microbiology & Infectious Diseases, 31, 337-345. Doi:10.1016/j.cimid.2007.04.002
  • Villamil, L, Figueras, A., Planas, M. & Novoa, B. (2010). Pediococcus acidilactici in the culture of turbot larvae: administration pathways. Aquaculture, 307(1-2), 83-88. Doi:10.1016/j.aquaculture.2010.07.004
Toplam 30 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Bölüm Makaleler
Yazarlar

Fatma Öztürk 0000-0003-4763-3801

Ömer Memduh Esendal 0000-0002-2700-2634

Proje Numarası Project No: TOVAG 104-O-496
Yayımlanma Tarihi 31 Mart 2020
Gönderilme Tarihi 10 Şubat 2020
Kabul Tarihi 13 Mart 2020
Yayımlandığı Sayı Yıl 2020 Cilt: 5 Sayı: 1

Kaynak Göster

APA Öztürk, F., & Esendal, Ö. M. (2020). Usage of Lactobacillus rhamnosus as a Probiotic in Sea Bass (Dicentrarchus labrax). Journal of Anatolian Environmental and Animal Sciences, 5(1), 93-99. https://doi.org/10.35229/jaes.687057
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