Oral Süspansiyondaki Sülfametoksazol ve Trimetoprimin Klasik Küçük Kare Kalibrasyon Metodu ile Spektrofotometrik Tayinleri ve Stabilite Çalışmaları
Öz
Oral süspansiyondaki sülfametoksazol ve trimetoprimin kantitatif tayini ve stabilite testi, spektrofotometrik klasik küçük kare kalibrasyon metodu ile çalışıldı. Sözkonusu veteriner ilaçlarının stabilite testleri, şu koşullarda gerçekleştirildi : Bir yıl süresince, oda ısısı, buzdolabı ve etüvde 37 0C bekletilen ilaçların, her üç ayda bir, analizleri yapıldı. Spektrofotometrik analiz için sülfametoksazol ve trimetoprimin, sırasıyla, 2.0-11.0 μg/mL ve 3.0-18.0 μg/mL konsantrasyon aralığındaki standart serileri hazırlandı. Standart serilerin absorbansları, üç ayrı dalga boyunda ölçüldü 237, 257 ve 288 nm . Bir klasik küçük kare kalibrasyonu, üç ayrı dalga boyunda absorbtiviteler ölçülerek elde edildi. Spektral metodun validasyonu, sentetik ikili karışımlar analiz edilerek yapıldı. Ardından da metot, sülfametoksazol ve trimetoprim içeren ticari veteriner formülasyonun eş zamanlı simultane analizi ve stabilite testine başarıyla uygulandı.
Anahtar Kelimeler
Spektral klasik küçük kare Simultane tayin Stabilite testi Sülfametoksazol Trimetoprim Kemometri
Kaynakça
- 1. Barragry, T.B. : “Chapter 12 : “Sulfonamides” in Veterinary Drug Therapy, Lea & Febiger, Philadelphia, Baltimore, Hong Kong, London, Munich, Sydney, Tokyo (1994), sayfa 297-301.
- 2. Budavari, S., O’Neil, M.J., Smith, A., Heckelman, P.E., Kinneary, JF. : The Merck Index. An Encyclopedia of Chemicals, Drugs and Biologicals. 12th ed., Merck Research Laboratories, Division of Merck & Co., Inc. Whitehouse Station, NJ (1996).
- 3. United States Pharmacopoiea USP 23, NF 18. United States Pharmacopoeial Convention Inc., Rockville (1995).
- 4. Bishop, Y.M. : The Veterinary Formulary, Handbook of Veterinary Medicines used in Veterinary Practice, 3rd ed., Bishop, Y.M. (Ed.), Royal Pharmaceutical Society of Great Britain and British Veterinary Association, London (1996), page 113.
- 5. Dinç, E., Baleanu, D., Kadıoğlu, Y., Kadıoğlu, E., Demirkaya, F. : New approach for simultaneous spectral analysis of a complex mixture using the fractional wavelet transform, Communications in Nonlinear Science and Numerical Simulation, 15 (4) : 812- 818 (2010).
- 6. Markopoulou, C.K., Malliou, E.T., Koundourellis, J.E. : Chemometric and derivative methods as flexible spectrophotometric approaches for dissolution and assaying tests in multicomponent tablets, Farmaco, 59 (8) : 627-636 (2004).
- 7. Granero, G., Garnero, C., Longhi, M. : Second derivative spectrophotometric determination of trimethoprime and sulfamethoxazole in the presence of hydroxypropyl-βcyclodextrine (HP-β-CD), J Pharm Biomed Anal, 29 (1-2) : 51-59 (2002).
- 8. Lopez-Martinez, L., Lopez-de-Alba, P.L., Manuel de-Leon-Rodriguez, L., Yepez Murrieta, M.L. : Simultaneous determination of binary mixtures of trimethoprim and sulfametoxasole or sulphamethoxypyridazine by the bivariate calibration spectrophotometric method, J Pharm Biomed Anal 30 (1) : 77-85 (2002).
- 9. Ribone, M.E., Pagani, A.P., Olivieri, A.C. : Determination of the minor component bromhexine in cotrimoxazole-containing tablets by absorbtion spectrophotometry and partial least-square (PLS-1) multivariate calibration, J Pharm Biomed Anal, 23 (2-3) : 591-595 (2000).
- 10. Dinç, E., Bilgili, A., Hanedan, B. : Simultaneous determination determination of trimethoprim and sulphamethoxasole in veterinary formulations by chromatographic multivariate methods, Pharmazie 62 (3) : 179-184 (2007).
- 11. Akay C., Özkan S.A. : Simultaneous LC determination of trimethoprim and sulfamethoxazole in pharmaceutical formulations, J Pharm Biomed Anal, 30 (4) : 1207- 1213 (2002).
- 12. Berzas Nevado, J.J., Castaneda Penalvo, G., Guzman Bernardo, F.J. : Simultaneous determination of sulfamethoxypyridazyne, sulfamethoxazole, sulfadimetoxine and their associated compounds by liquid chromatography, Anal Chim Acta, 442 (2) : 241- 248 (2001).
- 13. Berzas Nevado, J.J., Castaneda Penalvo, G., Guzman Bernardo, F.J. : Determination of sulfamethoxasole, sulfadiazine, and associated compounds in pharmaceutical preparations by capillary zone electrophoresis, J Chromatogr A, 918 (1) : 205-210 (2001).
Spectrophotometric Determination and Stability Studies of Sulfamethoxazole and Trimethoprim in Oral Suspension by Classical Least Square Calibration Method
Öz
Quantitative determination and stability test of sulfamethoxazole and trimethoprim in oral suspension were carried out by spectrophometric classical least square calibration method. Stability test of the related veterinary drugs was performed by using the following conditions: Room temperature, refrigerator and etuve 37 0C for each 3 month during 12 months. For the spectrophotometric analysis, the standard series of sulfamethoxazole and trimethoprim was prepared in the concentration range of 2.0-11.0 μg/mL and 3.0-18.0 μg/mL respectively. The absorbances of standard series were measured at three-wavelength set 237, 257 and 288 nm . A classical least square calibration was obtained by using the absorptivities at three-wavelength set. The validity of the spectral method was done by analyzing the synthetic binary mixtures. Af terwards the method was successfully applied to simultaneous analysis and stability test of the commercial veterinary formulation containing sulfamethoxazole and trimethoprim.
Anahtar Kelimeler
Spectral classical least square Simultaneous determination Stability test Sulfamethoxazole Trimethoprim Chemometry
Kaynakça
- 1. Barragry, T.B. : “Chapter 12 : “Sulfonamides” in Veterinary Drug Therapy, Lea & Febiger, Philadelphia, Baltimore, Hong Kong, London, Munich, Sydney, Tokyo (1994), sayfa 297-301.
- 2. Budavari, S., O’Neil, M.J., Smith, A., Heckelman, P.E., Kinneary, JF. : The Merck Index. An Encyclopedia of Chemicals, Drugs and Biologicals. 12th ed., Merck Research Laboratories, Division of Merck & Co., Inc. Whitehouse Station, NJ (1996).
- 3. United States Pharmacopoiea USP 23, NF 18. United States Pharmacopoeial Convention Inc., Rockville (1995).
- 4. Bishop, Y.M. : The Veterinary Formulary, Handbook of Veterinary Medicines used in Veterinary Practice, 3rd ed., Bishop, Y.M. (Ed.), Royal Pharmaceutical Society of Great Britain and British Veterinary Association, London (1996), page 113.
- 5. Dinç, E., Baleanu, D., Kadıoğlu, Y., Kadıoğlu, E., Demirkaya, F. : New approach for simultaneous spectral analysis of a complex mixture using the fractional wavelet transform, Communications in Nonlinear Science and Numerical Simulation, 15 (4) : 812- 818 (2010).
- 6. Markopoulou, C.K., Malliou, E.T., Koundourellis, J.E. : Chemometric and derivative methods as flexible spectrophotometric approaches for dissolution and assaying tests in multicomponent tablets, Farmaco, 59 (8) : 627-636 (2004).
- 7. Granero, G., Garnero, C., Longhi, M. : Second derivative spectrophotometric determination of trimethoprime and sulfamethoxazole in the presence of hydroxypropyl-βcyclodextrine (HP-β-CD), J Pharm Biomed Anal, 29 (1-2) : 51-59 (2002).
- 8. Lopez-Martinez, L., Lopez-de-Alba, P.L., Manuel de-Leon-Rodriguez, L., Yepez Murrieta, M.L. : Simultaneous determination of binary mixtures of trimethoprim and sulfametoxasole or sulphamethoxypyridazine by the bivariate calibration spectrophotometric method, J Pharm Biomed Anal 30 (1) : 77-85 (2002).
- 9. Ribone, M.E., Pagani, A.P., Olivieri, A.C. : Determination of the minor component bromhexine in cotrimoxazole-containing tablets by absorbtion spectrophotometry and partial least-square (PLS-1) multivariate calibration, J Pharm Biomed Anal, 23 (2-3) : 591-595 (2000).
- 10. Dinç, E., Bilgili, A., Hanedan, B. : Simultaneous determination determination of trimethoprim and sulphamethoxasole in veterinary formulations by chromatographic multivariate methods, Pharmazie 62 (3) : 179-184 (2007).
- 11. Akay C., Özkan S.A. : Simultaneous LC determination of trimethoprim and sulfamethoxazole in pharmaceutical formulations, J Pharm Biomed Anal, 30 (4) : 1207- 1213 (2002).
- 12. Berzas Nevado, J.J., Castaneda Penalvo, G., Guzman Bernardo, F.J. : Simultaneous determination of sulfamethoxypyridazyne, sulfamethoxazole, sulfadimetoxine and their associated compounds by liquid chromatography, Anal Chim Acta, 442 (2) : 241- 248 (2001).
- 13. Berzas Nevado, J.J., Castaneda Penalvo, G., Guzman Bernardo, F.J. : Determination of sulfamethoxasole, sulfadiazine, and associated compounds in pharmaceutical preparations by capillary zone electrophoresis, J Chromatogr A, 918 (1) : 205-210 (2001).
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Bölüm | Research Article |
| Yazarlar | |
| Yayımlanma Tarihi | 1 Haziran 2009 |
| Yayımlandığı Sayı | Yıl 2009 Sayı: 2 |