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The Effects of Rod and Round-Like Nanohydroxyapatites on Allium cepa Root Meristem Cells

Yıl 2024, Cilt: 37 Sayı: 1, 16 - 28, 01.03.2024

Merve Güneş Burcin Yalcin Ayşen Yağmur Kurşun Ghada Tagorti Emre Yavuz Esin Akarsu Nuray Kaya Bülent Kaya

https://doi.org/10.35378/gujs.1218829

Öz

Biomaterials are engineered products that are widely used in many areas of medicine fields such as orthopaedic applications, facial and maxillofacial surgery, artificial heart parts, metal parts, and implantable devices. These materials are widely used in medicine because they are biocompatible with the organism, non-allergic, and are resistant to physical and chemical factors. Hydroxyapatites are bioactive calcium phosphate ceramics that are compatible with tissues. Nano-sized hydroxyapatite has been produced to increase their bioactivity. Although there are advantages to the use of nanoparticles in medicine and therapy, the potential toxicity of these compounds on the ecosystem and human health are of concern. One of the key issues to be investigated is whether the different forms of the same nanoparticle will cause differences in genotoxicity. Herein, the potential genotoxic effects of rod and round forms of nano-sized hydroxyapatites (nHAs) were evaluated using the Allium cepa Single Cell Gel Electrophoresis (SCGE) method. Results had shown that the round form of nHA in the A. cepa meristem root tip cells caused statistically significant genotoxicity at 25 µg/mL concentration in terms of tail intensity and tail moment. This study indicated small-sized-nanohydroxyapatite-induced genotoxicity and cell death in A. cepa. This study has shown that the physical properties of nanoparticles affect potential toxicity mechanisms.

Anahtar Kelimeler

Allium cepa DNA damage Genotoxicity Root growth

Destekleyen Kurum

Scientific Research Projects Coordination Unit of Akdeniz University

Proje Numarası

FBA-2018-3285

Teşekkür

This study is supported by the Scientific Research Projects Coordination Unit of Akdeniz University (Project ID: FBA-2018-3285).

Kaynakça

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Yıl 2024, Cilt: 37 Sayı: 1, 16 - 28, 01.03.2024

Merve Güneş Burcin Yalcin Ayşen Yağmur Kurşun Ghada Tagorti Emre Yavuz Esin Akarsu Nuray Kaya Bülent Kaya

https://doi.org/10.35378/gujs.1218829

Öz

Proje Numarası

FBA-2018-3285

Kaynakça

  • [1] Suchanek, W., Yoshimura, M., “Processing and properties of hydroxyapatite-based biomaterials for use as hard tissue replacement implants”, Journal of Materials Research, 13(1): 94-117, (1998). https://doi.org/10.1557/JMR.1998.0015
  • [2] Phan, B.T.N., Nguyen, H.T., Dao, H.Q., Pham, L.V., Quan, T.T.T., Nguyen, D.B., Nguyen, H.T.L., Vu, T.T., “Synthesis and characterization of nano-hydroxyapatite in maltodextrin matrix”, Applied Nanoscience, 7:1–7, (2017). https://doi.org/10.1007/s13204-016-0541-z
  • [3] Ünal, F., Demırtaş Korkmaz, F., Suludere, Z., Erol, Ö., Yüzbaşıoğlu, D., “Genotoxicity of Two Nanoparticles: Titanium Dioxide and Zinc Oxide”, Gazi University Journal of Science, 34(4); 948-958, (2021). https://doi.org/10.35378/gujs.826911
  • [4] Behzadi, S., Serpooshan, V., Tao, W., Hamaly, M.A., Alkawareek, M.Y., Dreaden, E.C., Brown, D., Alkilany, A.M., Farokhzad, O.C., Mahmoudi, M., “Cellular uptake of nanoparticles: journey inside the cell”, Chemical Society Reviews, 46(14): 4218-4244, (2017). https://doi.org/10.1039/C6CS00636A
  • [5] Huang, Y., Cambre, M., Lee, H., “The Toxicity of Nanoparticles Depends on Multiple Molecular and Physicochemical Mechanisms”, International Journal of Molecular Sciences, 18(12): 2702, (2017). https://doi.org/10.3390/ijms18122702
  • [6] Carmona, E.R., Inostroza-Blancheteau, C., Rubıo, L., Marcos, R., “Genotoxicity of copper oxide nanoparticles in Drosophila melanogaster”, Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 791:1-11, (2015). https://10.1016/j.mrgentox.2015.07.006
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  • [8] Jantova, S., Theiszova, M., Letasiova, S., Birosova, L., Palou, T.M., “In vitro effects of fluor-hydroxyapatite, fluorapatite and hydroxyapatite on colony formation, DNA damage and mutagenicity”, Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 652(2), 139-44, (2008). https://doi.org/10.1016/j.mrgentox.2008.01.010
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  • [10] Liu, Z., Tang, S., Ai, Z., “Effects of hydroxyapatite nanoparticles on proliferation and apoptosis of human hepatoma BEL-7402 cells”, World Journal of Gastroenterology, 9(9): 1968-1971, (2003). https://doi.org/10.3748/wjg.v9.i9.1968
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Toplam 66 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Mühendislik
Bölüm Biology
Yazarlar

Merve Güneş 0000-0003-3278-0542

Burcin Yalcin 0000-0002-9694-5839

Ayşen Yağmur Kurşun 0000-0003-1657-6808

Ghada Tagorti 0000-0003-4597-8320

Emre Yavuz Bu kişi benim 0000-0001-9855-7437

Esin Akarsu 0000-0002-1965-5774

Nuray Kaya 0000-0002-3227-6680

Bülent Kaya 0000-0002-0491-9781

Proje Numarası FBA-2018-3285
Erken Görünüm Tarihi 4 Eylül 2023
Yayımlanma Tarihi 1 Mart 2024
Yayımlandığı Sayı Yıl 2024 Cilt: 37 Sayı: 1

Kaynak Göster

APA Güneş, M., Yalcin, B., Kurşun, A. Y., Tagorti, G., vd. (2024). The Effects of Rod and Round-Like Nanohydroxyapatites on Allium cepa Root Meristem Cells. Gazi University Journal of Science, 37(1), 16-28. https://doi.org/10.35378/gujs.1218829
AMA Güneş M, Yalcin B, Kurşun AY, Tagorti G, Yavuz E, Akarsu E, Kaya N, Kaya B. The Effects of Rod and Round-Like Nanohydroxyapatites on Allium cepa Root Meristem Cells. Gazi University Journal of Science. Mart 2024;37(1):16-28. doi:10.35378/gujs.1218829
Chicago Güneş, Merve, Burcin Yalcin, Ayşen Yağmur Kurşun, Ghada Tagorti, Emre Yavuz, Esin Akarsu, Nuray Kaya, ve Bülent Kaya. “The Effects of Rod and Round-Like Nanohydroxyapatites on Allium Cepa Root Meristem Cells”. Gazi University Journal of Science 37, sy. 1 (Mart 2024): 16-28. https://doi.org/10.35378/gujs.1218829.
EndNote Güneş M, Yalcin B, Kurşun AY, Tagorti G, Yavuz E, Akarsu E, Kaya N, Kaya B (01 Mart 2024) The Effects of Rod and Round-Like Nanohydroxyapatites on Allium cepa Root Meristem Cells. Gazi University Journal of Science 37 1 16–28.
IEEE M. Güneş, “The Effects of Rod and Round-Like Nanohydroxyapatites on Allium cepa Root Meristem Cells”, Gazi University Journal of Science, c. 37, sy. 1, ss. 16–28, 2024, doi: 10.35378/gujs.1218829.
ISNAD Güneş, Merve vd. “The Effects of Rod and Round-Like Nanohydroxyapatites on Allium Cepa Root Meristem Cells”. Gazi University Journal of Science 37/1 (Mart 2024), 16-28. https://doi.org/10.35378/gujs.1218829.
JAMA Güneş M, Yalcin B, Kurşun AY, Tagorti G, Yavuz E, Akarsu E, Kaya N, Kaya B. The Effects of Rod and Round-Like Nanohydroxyapatites on Allium cepa Root Meristem Cells. Gazi University Journal of Science. 2024;37:16–28.
MLA Güneş, Merve vd. “The Effects of Rod and Round-Like Nanohydroxyapatites on Allium Cepa Root Meristem Cells”. Gazi University Journal of Science, c. 37, sy. 1, 2024, ss. 16-28, doi:10.35378/gujs.1218829.
Vancouver Güneş M, Yalcin B, Kurşun AY, Tagorti G, Yavuz E, Akarsu E, Kaya N, Kaya B. The Effects of Rod and Round-Like Nanohydroxyapatites on Allium cepa Root Meristem Cells. Gazi University Journal of Science. 2024;37(1):16-28.