DMBA ile Meme Kanseri Oluşturulmuş Sıçanlarda Artan Uterus Doku Hasarı Çinko ve Melatonin Desteğiyle Önlenir
Öz
Amaç: Bu çalışmanın amacı dişi sıçanlarda DMBA ile oluşturulmuş meme kanserinde çinko ile melatonin uygulamasının uterus dokusundaki lipit peroksidasyonu üzerine olan etkilerinin araştırılmasıdır.Gereç ve Yöntem: Wistar cinsi sütten yeni kesilmiş toplam 42 adet dişi sıçan kullanılan araştırmada hayvanlar 5 gruba ayrıldı: Grup 1 Kontrol, Grup 2 DMBA Kontrol, Grup 3 DMBA+Çinko, Grup 4 DMBA+Melatonin, Grup 5 DMBA+Melatonin ve Çinko. Grup 1 dışındaki hayvanlara meme kanseri oluşturmak için kolza yağı kanola içinde 80 mg/kg dozunda DMBA gavaj yoluyla verildi. Çinko ve melatonin uygulanan gruplara 5 mg/kg/gün dozunda ip çinko, melatonin ve çinko+melatonin 4 hafta boyunca verildi. Hayvanlardan alınan uterus doku örneklerinde MDA malondialdehid ve GSH glutatyon düzeylerispektrofotometrik yöntemle tayin edildi.Bulgular: En yüksek uterus MDA seviyeleri DMBA ile meme kanseri oluşturulmuş kontrol G2 grubunda elde edildi p
Anahtar Kelimeler
Kaynakça
- Sofi MS, Sateesh MK, Bashir M, Ganie MA, Nabi S. Che- mopreventive and anti-breast cancer activity of compoun- ds isolated from leaves of Abrus precatorius L. 3 Biotech 2018;8(8):371.
- Misra R, Acharya S, Sahoo SK. Cancer nanotechnology: application of nanotechnology in cancer therapy. Drug Dis- cov Today 2010;15(19-20):842-50.
- Samy RP, Gopalakrishnakone P, Ignacimuthu S. Anti-tu- mor promoting potential of luteolin against 7,12-dimet- hylbenz(a)anthracene-induced mammary tumors in rats. Chem Biol Interact 2006;164(1-2):1-14.
- Russo J, Russo IH. Experimentally induced mammary tu- mors in rats. Breast Cancer Res Treat. 1996;39(1):7-20.
- Izzotti A, Camoirano A, Cartiglia C, Grubbs CJ, Lubet RA, Kelloff GJ, Flora SD. Patterns of DNA adduct formation in liver and mammary epithelial cells of rats treated with 7, 12-dimethylbenz(a)anthracene, and selective effects of che- mopreventive agents. Can Res 1999;59:4285–90.
- Kumar R, Razab S, Prabhu K, Ray S, Prakash B. Serum but- yrylcholinesterase and zinc in breast cancer. J Cancer Res Ther 2017;13(2):367-70.
- Cui N, Hu M, Khalil RA. Biochemical and Biological Attri- butes of Matrix Metalloproteinases. Prog Mol Biol Transl Sci 2017;147:1-73.
- da Cruz RS, Andrade FO, Carioni VMO, Rosim MP, Miran- da MLP, Fontelles CC, de Oliveira PV, Barbisan LF, Castro IA, Ong TP. Dietary zinc deficiency or supplementation during gestation increases breast cancer susceptibility in adult female mice offspring following a J-shaped pattern and through distinct mechanisms. Food Chem Toxicol 2019;134:110813.
- Ferreira LC, Orso F, Dettori D, Lacerda JZ, Borin TF, Ta- verna D, Zuccari DAPC.The role of melatonin on miRNAs modulation in triple-negative breast cancer cells. PLoS One 2020;15(2):e0228062.
- Kubatka P, Zubor P, Busselberg D, Kwon TK, Adamek M, Petrovic D, Opatrilova R, Gazdikova K, Caprnda M, Rodri- go L, Danko J, Kruzliak P. Melatonin and breast cancer: Evi- dences from preclinical and human studies. Crit Rev Oncol Hematol 2018;122:133-43.
- Akhtar A, Ratra A, Puckett Y, Sheikh AB, Ronaghan CA. Synchronous Uterine Metastases from Breast Cancer: Case Study and Literature Review. Cureus 2017;9(11):e1840.
- Çift T, Aslan B, Bulut B, İlvan Ş. Unusual uterine metastasis of invasive ductal carcinoma: A case report. Turk J Obstet Gynecol 2016;13(3):164-6.
- Mihara M, Uchiyama M. Determination of malonaldehyde precursor in tissues by thiobarbituric acid test.Anal Bioc- hem 1978;86(1):271-8.
- Sedlak J, Lindsay RH. Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent.Anal Biochem 1968;25(1):192-205.
- Gan H, Zhang Y, Zhou Q, Zheng L, Xie X, Veeraraghavan VP, Mohan SK. Zingerone induced caspase-dependent apoptosis in MCF-7 cells and prevents 7,12-dimethylben- z(a) anthracene-induced mammary carcinogenesis in expe- rimental rats. J Biochem Mol Toxicol 2019 ;33(10):e22387.
- Rajendran J, Pachaiappan P, Subramaniyan S. Dose-de- pendent chemopreventive effects of citronellol in DM- BA-induced breast cancer among rats. Drug Dev Res 2019;80(6):867-76.
- Zeweil MM, Sadek KM, Taha NM, El-Sayed Y, Menshawy S. Graviola attenuates DMBA-induced breast cancer possibly through augmenting apoptosis and antioxidant pathway and downregulating estrogen receptors. Environ Sci Pollut Res Int 2019;26(15):15209-17.
- Hecht F, Pessoa CF, Gentile LB, Rosenthal D, Carvalho DP, Fortunato RS. The role of oxidative stress on breast cancer development and therapy. Tumour Biol 2016;37(4):4281- 91.
- Gurer-Orhan H, Ince E, Konyar D, Saso L, Suzen S. The Role of Oxidative Stress Modulators in Breast Cancer. Curr Med Chem 2018;25(33):4084-101.
- Borges de Araşjo CG, Oliveira do Nascimento Holanda A, de Souza Rocha CV, Soares do Nascimento AP, Simplício Revoredo CM, Borges da Silva B, do Nascimento Nogue- ira N, do Nascimento Marreiro D. Relationship between zincemia, superoxide dismutase activity and marker of oxidatıve stress in women with breast cancer. Nutr Hosp 2015;32(2):785-91.
- Famurewa AC, Ekeleme-Egedigwe CA, David EE, Eleazu CO, Folawiyo AM, Obasi NA. Zinc abrogates anticancer drug tamoxifen-induced hepatotoxicity by suppressing redox imbalance, NO/iNOS/NF-ĸB signaling, and cas- pase-3-dependent apoptosis in female rats. Toxicol Mech Methods 2020;30(2):115-23.
- Amin AH1, El-Missiry MA, Othman AI, Ali DA, Gouida MS, Ismail AH. Ameliorative effects of melatonin against solid Ehrlich carcinoma progression in female mice. J Pine- al Res 2019;67(2):e12585.
- Baltaci SB, Mogulkoc R, Baltaci AK, Emsen A, Artac H. The effect of zinc and melatonin supplementation on immunity parameters in breast cancer induced by DMBA in rats. Arch Physiol Biochem 2018;124(3):247-52.
- Luo M, Shang L, Brooks MD, Jiagge E, Zhu Y, Buschhaus JM, Conley S, Fath MA, Davis A, Gheordunescu E, Wang Y, Harouaka R, Lozier A, Triner D, McDermott S, Meraj- ver SD, Luker GD, Spitz DR, Wicha MS. Targeting Breast Cancer Stem Cell State Equilibrium through Modulation of Redox Signaling. Cell Metab 2018;28(1):69-86.e6.
- Didžiapetrienė J, Kazbarienė B, Tikuišis R, Dulskas A, Dab- kevičienė D, Lukosevičienė V, Kontrimavičiūtė E, Sužiedėlis K, Ostapenko V. Oxidant/Antioxidant Status of Breast Can- cer Patients in Pre- and Post-Operative Periods. Medicina (Kaunas) 2020;56(2). pii: E70.
- Gumulec J, Masarik M, Krizkova S, Adam V, Hubalek J, Hrabeta J, Eckschlager T, Stiborova M, Kizek R. Insight to physiology and pathology of zinc(II) ions and their ac- tions in breast and prostate carcinoma. Curr Med Chem 2011;18(33):5041-51.
- Al-Saran N, Subash-Babu P, Al-Nouri DM, Alfawaz HA, Alshatwi AA. Zinc enhances CDKN2A, pRb1 expression and regulates functional apoptosis via upregulation of p53 and p21 expression in human breast cancer MCF-7 cell. En- viron Toxicol Pharmacol 2016;47:19-27.
- Jarosz M, Olbert M, Wyszogrodzka G, Młyniec K, Librows- ki T. Antioxidant and anti-inflammatory effects of zinc. Zinc-dependent NF-κB signaling. Inflammopharmacology 2017;25(1):11-24.
- Li Y, Li S, Zhou Y, Meng X, Zhang JJ, Xu DP, Li HB. Melato- nin for the prevention and treatment of cancer. Oncotarget 2017;8(24):39896-921.
- Hill SM, Belancio VP, Dauchy RT, Xiang S, Brimer S, Mao L, Hauch A, Lundberg PW, Summers W, Yuan L, Frasch T, Blask DE. Melatonin: an inhibitor of breast cancer. Endocr Relat Cancer 2015;22(3):R183-204.
Zinc and melatonin supplementation ameliorates uterus tissue damage in dmba-indu- ced breast cancer in rats
Öz
Objective: This study aims to investigate the effects of zinc and melatonin supplementation on lipid peroxidation in the uterus in DMBA-induced breast cancer in female rats.Material and Methods: A total of 42 recently weaned Wistar rats were divided into 5 groups as follows: Control Group 1 , DMBA Control Group 2 , DMBA + Zinc Group 3 , DMBA + Melatonin Group 4 , DMBA + Melatonin & Zinc Group 5 . 80 mg/kg of DMBA was given in canola oil through gavage to animals except for group 1 to induce breast cancer. 5 mg/kg of zinc and/or melatonin were given daily for 4 weeks intraperitoneally to related groups. Malondialdehyde MDA and glutathione GSH levels in uterus tissue samples were determined via spectrophotometric methods.Results: The highest uterus tissue MDA levels were in the DMBA-treated group Group 2 p
Anahtar Kelimeler
Kaynakça
- Sofi MS, Sateesh MK, Bashir M, Ganie MA, Nabi S. Che- mopreventive and anti-breast cancer activity of compoun- ds isolated from leaves of Abrus precatorius L. 3 Biotech 2018;8(8):371.
- Misra R, Acharya S, Sahoo SK. Cancer nanotechnology: application of nanotechnology in cancer therapy. Drug Dis- cov Today 2010;15(19-20):842-50.
- Samy RP, Gopalakrishnakone P, Ignacimuthu S. Anti-tu- mor promoting potential of luteolin against 7,12-dimet- hylbenz(a)anthracene-induced mammary tumors in rats. Chem Biol Interact 2006;164(1-2):1-14.
- Russo J, Russo IH. Experimentally induced mammary tu- mors in rats. Breast Cancer Res Treat. 1996;39(1):7-20.
- Izzotti A, Camoirano A, Cartiglia C, Grubbs CJ, Lubet RA, Kelloff GJ, Flora SD. Patterns of DNA adduct formation in liver and mammary epithelial cells of rats treated with 7, 12-dimethylbenz(a)anthracene, and selective effects of che- mopreventive agents. Can Res 1999;59:4285–90.
- Kumar R, Razab S, Prabhu K, Ray S, Prakash B. Serum but- yrylcholinesterase and zinc in breast cancer. J Cancer Res Ther 2017;13(2):367-70.
- Cui N, Hu M, Khalil RA. Biochemical and Biological Attri- butes of Matrix Metalloproteinases. Prog Mol Biol Transl Sci 2017;147:1-73.
- da Cruz RS, Andrade FO, Carioni VMO, Rosim MP, Miran- da MLP, Fontelles CC, de Oliveira PV, Barbisan LF, Castro IA, Ong TP. Dietary zinc deficiency or supplementation during gestation increases breast cancer susceptibility in adult female mice offspring following a J-shaped pattern and through distinct mechanisms. Food Chem Toxicol 2019;134:110813.
- Ferreira LC, Orso F, Dettori D, Lacerda JZ, Borin TF, Ta- verna D, Zuccari DAPC.The role of melatonin on miRNAs modulation in triple-negative breast cancer cells. PLoS One 2020;15(2):e0228062.
- Kubatka P, Zubor P, Busselberg D, Kwon TK, Adamek M, Petrovic D, Opatrilova R, Gazdikova K, Caprnda M, Rodri- go L, Danko J, Kruzliak P. Melatonin and breast cancer: Evi- dences from preclinical and human studies. Crit Rev Oncol Hematol 2018;122:133-43.
- Akhtar A, Ratra A, Puckett Y, Sheikh AB, Ronaghan CA. Synchronous Uterine Metastases from Breast Cancer: Case Study and Literature Review. Cureus 2017;9(11):e1840.
- Çift T, Aslan B, Bulut B, İlvan Ş. Unusual uterine metastasis of invasive ductal carcinoma: A case report. Turk J Obstet Gynecol 2016;13(3):164-6.
- Mihara M, Uchiyama M. Determination of malonaldehyde precursor in tissues by thiobarbituric acid test.Anal Bioc- hem 1978;86(1):271-8.
- Sedlak J, Lindsay RH. Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent.Anal Biochem 1968;25(1):192-205.
- Gan H, Zhang Y, Zhou Q, Zheng L, Xie X, Veeraraghavan VP, Mohan SK. Zingerone induced caspase-dependent apoptosis in MCF-7 cells and prevents 7,12-dimethylben- z(a) anthracene-induced mammary carcinogenesis in expe- rimental rats. J Biochem Mol Toxicol 2019 ;33(10):e22387.
- Rajendran J, Pachaiappan P, Subramaniyan S. Dose-de- pendent chemopreventive effects of citronellol in DM- BA-induced breast cancer among rats. Drug Dev Res 2019;80(6):867-76.
- Zeweil MM, Sadek KM, Taha NM, El-Sayed Y, Menshawy S. Graviola attenuates DMBA-induced breast cancer possibly through augmenting apoptosis and antioxidant pathway and downregulating estrogen receptors. Environ Sci Pollut Res Int 2019;26(15):15209-17.
- Hecht F, Pessoa CF, Gentile LB, Rosenthal D, Carvalho DP, Fortunato RS. The role of oxidative stress on breast cancer development and therapy. Tumour Biol 2016;37(4):4281- 91.
- Gurer-Orhan H, Ince E, Konyar D, Saso L, Suzen S. The Role of Oxidative Stress Modulators in Breast Cancer. Curr Med Chem 2018;25(33):4084-101.
- Borges de Araşjo CG, Oliveira do Nascimento Holanda A, de Souza Rocha CV, Soares do Nascimento AP, Simplício Revoredo CM, Borges da Silva B, do Nascimento Nogue- ira N, do Nascimento Marreiro D. Relationship between zincemia, superoxide dismutase activity and marker of oxidatıve stress in women with breast cancer. Nutr Hosp 2015;32(2):785-91.
- Famurewa AC, Ekeleme-Egedigwe CA, David EE, Eleazu CO, Folawiyo AM, Obasi NA. Zinc abrogates anticancer drug tamoxifen-induced hepatotoxicity by suppressing redox imbalance, NO/iNOS/NF-ĸB signaling, and cas- pase-3-dependent apoptosis in female rats. Toxicol Mech Methods 2020;30(2):115-23.
- Amin AH1, El-Missiry MA, Othman AI, Ali DA, Gouida MS, Ismail AH. Ameliorative effects of melatonin against solid Ehrlich carcinoma progression in female mice. J Pine- al Res 2019;67(2):e12585.
- Baltaci SB, Mogulkoc R, Baltaci AK, Emsen A, Artac H. The effect of zinc and melatonin supplementation on immunity parameters in breast cancer induced by DMBA in rats. Arch Physiol Biochem 2018;124(3):247-52.
- Luo M, Shang L, Brooks MD, Jiagge E, Zhu Y, Buschhaus JM, Conley S, Fath MA, Davis A, Gheordunescu E, Wang Y, Harouaka R, Lozier A, Triner D, McDermott S, Meraj- ver SD, Luker GD, Spitz DR, Wicha MS. Targeting Breast Cancer Stem Cell State Equilibrium through Modulation of Redox Signaling. Cell Metab 2018;28(1):69-86.e6.
- Didžiapetrienė J, Kazbarienė B, Tikuišis R, Dulskas A, Dab- kevičienė D, Lukosevičienė V, Kontrimavičiūtė E, Sužiedėlis K, Ostapenko V. Oxidant/Antioxidant Status of Breast Can- cer Patients in Pre- and Post-Operative Periods. Medicina (Kaunas) 2020;56(2). pii: E70.
- Gumulec J, Masarik M, Krizkova S, Adam V, Hubalek J, Hrabeta J, Eckschlager T, Stiborova M, Kizek R. Insight to physiology and pathology of zinc(II) ions and their ac- tions in breast and prostate carcinoma. Curr Med Chem 2011;18(33):5041-51.
- Al-Saran N, Subash-Babu P, Al-Nouri DM, Alfawaz HA, Alshatwi AA. Zinc enhances CDKN2A, pRb1 expression and regulates functional apoptosis via upregulation of p53 and p21 expression in human breast cancer MCF-7 cell. En- viron Toxicol Pharmacol 2016;47:19-27.
- Jarosz M, Olbert M, Wyszogrodzka G, Młyniec K, Librows- ki T. Antioxidant and anti-inflammatory effects of zinc. Zinc-dependent NF-κB signaling. Inflammopharmacology 2017;25(1):11-24.
- Li Y, Li S, Zhou Y, Meng X, Zhang JJ, Xu DP, Li HB. Melato- nin for the prevention and treatment of cancer. Oncotarget 2017;8(24):39896-921.
- Hill SM, Belancio VP, Dauchy RT, Xiang S, Brimer S, Mao L, Hauch A, Lundberg PW, Summers W, Yuan L, Frasch T, Blask DE. Melatonin: an inhibitor of breast cancer. Endocr Relat Cancer 2015;22(3):R183-204.
Toplam 30 adet kaynakça vardır.
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Bölüm | Original Article |
| Yazarlar | |
| Yayımlanma Tarihi | 1 Eylül 2020 |
| Yayımlandığı Sayı | Yıl 2020 Cilt: 30 Sayı: 3 |
Genel Tıp Dergisi Creative Commons Atıf-GayriTicari 4.0 Uluslararası Lisansı (CC BY NC) ile lisanslanmıştır.