Protective Effect of Silymarin and Boric Acid Against Isoproterenol-Induced Myocardial Infarction in Mice
Öz
This study aimed to compare the protective or therapeutic effects of silymarin and/or boric acid against myocardial infarction induced by isoproterenol. Distilled water was given orally by gavage to the GI and GII groups for fourteen days. The GIII group was received silymarin (100mg/kg), while the GIV group was given boric acid (100mg/kg) for 14 days. In the last group, both silymarin and boric acid were given orally (gavage) for fourteen days. On the thirteenth and fourteenth days of the study, while isotonic water was applied to the GI group, isoproterenol (85mg/kg) was applied to the other groups subcutaneously. Plasma ALT, AST, Ca, CK, CHO, TP, Ing P, IL-6, IL-12, PAL-1, and sPLA2 parameters were evaluated. Histopathological examination was performed on the heart, lung, liver, kidney, spleen, cerebrum, and cerebellum tissues taken from mice. The CK level was found to be significantly lower in the GIII and GV groups compared to the GII group (P<0.05). As a result, the increases shown in the plasma AST and CK activities caused due to the myocardial damage induced by isoproterenol were decreased in the treatment groups. Therefore it was thought that silymarin and boric acid may contribute to preventive treatment against myocardial infarction.
Anahtar Kelimeler
Boric acid Creatinine kinase Isoproteranol Myocardial infarction Silymarin
Kaynakça
- Abbas AM. (2016) Cardioprotective effect of resveratrol analogue isorhapontigenin versus omega-3 fatty acids in isoproterenol-induced myocardial infarction in rats. J Physiol Biochem. 72, 469-484.
- Chambers CS, Holečková V, Petrásková L, Biedermann D, Valentová K, Buchta M, Křen V. (2017) The silymarin composition… and why does it matter???. Food Res Int. 100(3), 339-353.
- De Sánchez CV, Yañez-Maldonado L, Vidrio-Gómez S et al. Role of nitric oxide in isoproterenol-induced myocardial infarction. In: Fiuza M (Ed.). Cardiotoxicity of Oncologic Treatments, IntechOpen, 2012, DOI: 10.5772/34505.
- Devirian TA, Volpe SL. (2003) The physiological effects of dietary boron. Crit Rev Food Sci Nutr. 43(2), 219-231.
- Devrim AK, Sozmen M, Devrim T, Sudagidan M, Cinar M, Kabak YB. (2014) Periostin normalizes levels of cardiac markers in rats with experimental isoproterenol cardiotoxicity. Bratisl Lek Listy. 2017; 118(11): 705-709.
- Ekor M. The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety. Front Pharmacol. 4, 177.
- Karimkhani H, Özkoç M, Shojaolsadati P, Uzuner K, Donmez DB, Kanbak G. (2021) Protective Effect of Boric Acid and Omega-3 on Myocardial Infarction in an Experimental Rat Model. Biol Trace Elem Res. 199(7), 2612-2620.
- Karthick M, Stanely Mainzen Prince P. (2006) Preventive effect of rutin, a bioflavonoid, on lipid peroxides and antioxidants in isoproterenol-induced myocardial infarction in rats. J Pharm Pharmacol. 58(5), 701-707.
- Lalitha G, Poornima P, Archanah A, Padma VV. (2013) Protective effect of neferine against isoproterenol-induced cardiac toxicity. Cardiovasc Toxicol. 13(2), 168-179.
- Liu C, Huang Y. (2016) Chinese Herbal Medicine on Cardiovascular Diseases and the Mechanisms of Action. Front Pharmacol. 7, 469.
- Lu L, Liu M, Sun R, Zheng Y, Zhang P. (2015) Myocardial Infarction: Symptoms and Treatments. Cell Biochem Biophys. 72(3), 865-867.
- Luna LG. Manual of histologic staining methods of the armed forces ınstitute of pathology. 3rd Ed., McGraw-Hill, New York, USA. 1968.
- MacDonald-Ramos K, Michán L, Martínez-Ibarra A, Cerbón M. (2021) Silymarin is an ally against insulin resistance: A review. Ann Hepatol. 23, 100255.
- Mathew S, Menon PV, Kurup PA. (1985) Effect of administration of vitamin A, ascorbic acid and nicotinamide adenine dinucleotide + flavin adenine dinucleotide on severity of myocardial infarction induced by isoproterenol in rats. Indian J Exp Biol. 23(9), 500-504.
- McCullough ML, Peterson JJ, Patel R, Jacques PF, Shah R, Dwyer JT. (2012) Flavonoid intake and cardiovascular disease mortality in a prospective cohort of US adults. Am J Clin Nutr. 95(2), 454-464.
- Mert H, Yılmaz H, Irak K, Yıldırım S, Mert N. (2018) Investigation of the Protective Effect of Kefir against Isoproterenol Induced Myocardial Infarction in Rats. Korean J Food Sci Anim Resour. 38(2), 259-272.
- Mukherjee D, Roy SG, Bandyopadhyay A, Chattopadhyay A, Basu A, Mitra E, Ghosh AK, Reiter RJ, Bandyopadhyay D. (2010) Melatonin protects against isoproterenol-induced myocardial injury in the rat: antioxidative mechanisms. J Pineal Res. 48(3), 251-262.
- Murray FJ. (1998) A comparative review of the pharmacokinetics of boric acid in rodents and humans. Biol Trace Elem Res. 66(1-3), 331-41.
- Neri M, Fineschi V, Di Paolo M, Pomara C, Riezzo I, Turillazzi E, Cerretani D. (2015) Cardiac oxidative stress and inflammatory cytokines response after myocardial infarction. Curr Vasc Pharmacol. 13(1), 26-36.
- Nivethetha M, Jayasri J, Brindha P. (2009) Effects of Muntingia calabura L. on isoproterenol-induced myocardial infarction. Singapore Med J. 50(3), 300-302.
- Papackova Z, Heczkova M, Dankova H, Sticova E, Lodererova A, Bartonova L, Poruba M, Cahova M. (2018) Silymarin prevents acetaminophen-induced hepatotoxicity in mice. PLoS One. 13(1), e0191353.
- Pérez-Cao A, Gil-Loyzaga P, Merchàn-Pérez A, Tamargo J. (1994) Effects of oxodipine and nitrendipine on the size of experimental myocardial infarct in the rat. Pharmacol Toxicol. 74(6), 321-329.
- Pollard TJ. (2000) The acute myocardial infarction. Prim Care. 27(3), 631-649.
- Priscilla DH, Prince PS. (2009) Cardioprotective effect of gallic acid on cardiac troponin-T, cardiac marker enzymes, lipid peroxidation products and antioxidants in experimentally induced myocardial infarction in Wistar rats. Chem Biol Interact. 179(2-3), 118-124.
- Rona G. (1985) Catecholamine cardiotoxicity. J Mol Cell Cardiol. 17(4), 291-306.
- Saleh M, Ambrose JA. (2018) Understanding myocardial infarction. F1000Res. 7:F1000 Faculty Rev-1378.
- Shaito A, Thuan DTB, Phu HT, Nguyen THD, Hasan H, Halabi S, Abdelhady S, Nasrallah GK, Eid AH, Pintus G. (2020) Herbal Medicine for Cardiovascular Diseases: Efficacy, Mechanisms, and Safety. Front Pharmacol. 11, 422.
- Sugamura K, Keaney JF. (2011) Reactive oxygen species in cardiovascular disease. Free Radic. Biol. Med. 51(5), 978–992.
- Tiwari R, Mohan M, Kasture S, Maxia A, Ballero M. (2009) Cardioprotective potential of myricetin in isoproterenol-induced myocardial infarction in Wistar rats. Phytother Res. 23(10), 1361-1366.
- Uluisik I, Karakaya HC, Koc A. (2018) The importance of boron in biological systems. J Trace Elem Med Biol. 45, 156-162.
- Vahabzadeh M, Amiri N, Karimi G. (2018) Effects of silymarin on metabolic syndrome: a review. J Sci Food Agric. 98(13), 4816-4823.
- Zhou FT, Ma K. (2020) Fasudil protects against isoproterenol-induced myocardial infarction in mice via inhibiting Rho/ROCK signaling pathway. Eur Rev Med Pharmacol Sci. 24(10), 5659-5667.
Farelerde İzoproterenol ile İndüklenmiş Miyokard İnfarktüsüne Karşı Silimarin ve Borik Asidin Koruyucu Etkisi
Öz
Bu çalışmada izoproterenol ile oluşturulan miyokard infarktüsüne karşı silimarin ve/veya borik asidin koruyucu veya tedavi edici etkilerinin karşılaştırılması amaçlanmıştır. GI ve GII gruplarına on dört gün boyunca gavaj ile oral yolla distile su verildi. On dört gün boyunca; GIII grubuna silimarin (100mg/kg) verilirken GIV grubuna borik asit (100mg/kg) verildi. Son gruba ise hem silimarin hem de borik asit on dört gün boyunca oral yolla (gavaj) verildi. Çalışmanın on üçüncü ve on dördüncü günü intraperitoneal yolla; GI grubuna izotonik uygulanırken, diğer gruplara subkutan izoproteranol (85mg/kg) uygulandı. Plazmadan; ALT, AST, Ca, CK, CHO, TP, Ing P, IL-6, IL-12, PAL-1 ve sPLA2 parametreleri değerlendirildi. Farelerden alınan kalp, akciğer, karaciğer, böbrek, dalak, cerebrum ve cerebellum dokularında histopatolojik inceleme yapıldı. CK düzeyi GII grubuna göre GIII grubunda anlamlı düzeyde azaldığı bulundu (P<0,05). Sonuç olarak isoproterenolun sebep olduğu miyokardiyal hasar sonucunda AST ve CK düzeylerinde artışlarına karşı tedavi gruplarında azalmalar görüldü. Bu özelliklerinden dolayı, silymarin ve boric asitin; miyokard enfarktüs durumlarına karşı koruyucu tedaviye katkı sağlayabileceği düşünülmektedir.
Anahtar Kelimeler
Borik asit İzoproteranol Kreatin kinaz Miyokard enfraktüsü Silimarin
Kaynakça
- Abbas AM. (2016) Cardioprotective effect of resveratrol analogue isorhapontigenin versus omega-3 fatty acids in isoproterenol-induced myocardial infarction in rats. J Physiol Biochem. 72, 469-484.
- Chambers CS, Holečková V, Petrásková L, Biedermann D, Valentová K, Buchta M, Křen V. (2017) The silymarin composition… and why does it matter???. Food Res Int. 100(3), 339-353.
- De Sánchez CV, Yañez-Maldonado L, Vidrio-Gómez S et al. Role of nitric oxide in isoproterenol-induced myocardial infarction. In: Fiuza M (Ed.). Cardiotoxicity of Oncologic Treatments, IntechOpen, 2012, DOI: 10.5772/34505.
- Devirian TA, Volpe SL. (2003) The physiological effects of dietary boron. Crit Rev Food Sci Nutr. 43(2), 219-231.
- Devrim AK, Sozmen M, Devrim T, Sudagidan M, Cinar M, Kabak YB. (2014) Periostin normalizes levels of cardiac markers in rats with experimental isoproterenol cardiotoxicity. Bratisl Lek Listy. 2017; 118(11): 705-709.
- Ekor M. The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety. Front Pharmacol. 4, 177.
- Karimkhani H, Özkoç M, Shojaolsadati P, Uzuner K, Donmez DB, Kanbak G. (2021) Protective Effect of Boric Acid and Omega-3 on Myocardial Infarction in an Experimental Rat Model. Biol Trace Elem Res. 199(7), 2612-2620.
- Karthick M, Stanely Mainzen Prince P. (2006) Preventive effect of rutin, a bioflavonoid, on lipid peroxides and antioxidants in isoproterenol-induced myocardial infarction in rats. J Pharm Pharmacol. 58(5), 701-707.
- Lalitha G, Poornima P, Archanah A, Padma VV. (2013) Protective effect of neferine against isoproterenol-induced cardiac toxicity. Cardiovasc Toxicol. 13(2), 168-179.
- Liu C, Huang Y. (2016) Chinese Herbal Medicine on Cardiovascular Diseases and the Mechanisms of Action. Front Pharmacol. 7, 469.
- Lu L, Liu M, Sun R, Zheng Y, Zhang P. (2015) Myocardial Infarction: Symptoms and Treatments. Cell Biochem Biophys. 72(3), 865-867.
- Luna LG. Manual of histologic staining methods of the armed forces ınstitute of pathology. 3rd Ed., McGraw-Hill, New York, USA. 1968.
- MacDonald-Ramos K, Michán L, Martínez-Ibarra A, Cerbón M. (2021) Silymarin is an ally against insulin resistance: A review. Ann Hepatol. 23, 100255.
- Mathew S, Menon PV, Kurup PA. (1985) Effect of administration of vitamin A, ascorbic acid and nicotinamide adenine dinucleotide + flavin adenine dinucleotide on severity of myocardial infarction induced by isoproterenol in rats. Indian J Exp Biol. 23(9), 500-504.
- McCullough ML, Peterson JJ, Patel R, Jacques PF, Shah R, Dwyer JT. (2012) Flavonoid intake and cardiovascular disease mortality in a prospective cohort of US adults. Am J Clin Nutr. 95(2), 454-464.
- Mert H, Yılmaz H, Irak K, Yıldırım S, Mert N. (2018) Investigation of the Protective Effect of Kefir against Isoproterenol Induced Myocardial Infarction in Rats. Korean J Food Sci Anim Resour. 38(2), 259-272.
- Mukherjee D, Roy SG, Bandyopadhyay A, Chattopadhyay A, Basu A, Mitra E, Ghosh AK, Reiter RJ, Bandyopadhyay D. (2010) Melatonin protects against isoproterenol-induced myocardial injury in the rat: antioxidative mechanisms. J Pineal Res. 48(3), 251-262.
- Murray FJ. (1998) A comparative review of the pharmacokinetics of boric acid in rodents and humans. Biol Trace Elem Res. 66(1-3), 331-41.
- Neri M, Fineschi V, Di Paolo M, Pomara C, Riezzo I, Turillazzi E, Cerretani D. (2015) Cardiac oxidative stress and inflammatory cytokines response after myocardial infarction. Curr Vasc Pharmacol. 13(1), 26-36.
- Nivethetha M, Jayasri J, Brindha P. (2009) Effects of Muntingia calabura L. on isoproterenol-induced myocardial infarction. Singapore Med J. 50(3), 300-302.
- Papackova Z, Heczkova M, Dankova H, Sticova E, Lodererova A, Bartonova L, Poruba M, Cahova M. (2018) Silymarin prevents acetaminophen-induced hepatotoxicity in mice. PLoS One. 13(1), e0191353.
- Pérez-Cao A, Gil-Loyzaga P, Merchàn-Pérez A, Tamargo J. (1994) Effects of oxodipine and nitrendipine on the size of experimental myocardial infarct in the rat. Pharmacol Toxicol. 74(6), 321-329.
- Pollard TJ. (2000) The acute myocardial infarction. Prim Care. 27(3), 631-649.
- Priscilla DH, Prince PS. (2009) Cardioprotective effect of gallic acid on cardiac troponin-T, cardiac marker enzymes, lipid peroxidation products and antioxidants in experimentally induced myocardial infarction in Wistar rats. Chem Biol Interact. 179(2-3), 118-124.
- Rona G. (1985) Catecholamine cardiotoxicity. J Mol Cell Cardiol. 17(4), 291-306.
- Saleh M, Ambrose JA. (2018) Understanding myocardial infarction. F1000Res. 7:F1000 Faculty Rev-1378.
- Shaito A, Thuan DTB, Phu HT, Nguyen THD, Hasan H, Halabi S, Abdelhady S, Nasrallah GK, Eid AH, Pintus G. (2020) Herbal Medicine for Cardiovascular Diseases: Efficacy, Mechanisms, and Safety. Front Pharmacol. 11, 422.
- Sugamura K, Keaney JF. (2011) Reactive oxygen species in cardiovascular disease. Free Radic. Biol. Med. 51(5), 978–992.
- Tiwari R, Mohan M, Kasture S, Maxia A, Ballero M. (2009) Cardioprotective potential of myricetin in isoproterenol-induced myocardial infarction in Wistar rats. Phytother Res. 23(10), 1361-1366.
- Uluisik I, Karakaya HC, Koc A. (2018) The importance of boron in biological systems. J Trace Elem Med Biol. 45, 156-162.
- Vahabzadeh M, Amiri N, Karimi G. (2018) Effects of silymarin on metabolic syndrome: a review. J Sci Food Agric. 98(13), 4816-4823.
- Zhou FT, Ma K. (2020) Fasudil protects against isoproterenol-induced myocardial infarction in mice via inhibiting Rho/ROCK signaling pathway. Eur Rev Med Pharmacol Sci. 24(10), 5659-5667.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Veteriner Bilimleri |
| Bölüm | Araştırma Makaleleri |
| Yazarlar | |
| Erken Görünüm Tarihi | 24 Haziran 2022 |
| Yayımlanma Tarihi | 15 Haziran 2022 |
| Gönderilme Tarihi | 25 Mart 2022 |
| Yayımlandığı Sayı | Yıl 2022 Cilt: 33 Sayı: 1 |
