Investigation of Serotonin 2A Receptor Gene 5-HTR2A rs6311 Polymorphism in Trigeminal Neuralgia Disease
Öz
Objectives: Trigeminal neuralgia (TN) is a disease that occurs in the distribution region of the trigeminal nerve and is frequently recurrent pain. Serotonin (5-HT) acts on neurotransmitters in the central nervous system. The study aims to examine the relationship between sensitivity and pain intensity of the serotonin receptor gene (5-HTR2A) and rs6311 polymorphism in patients by examining TN genetically.
Materials and Methods: The study consisted of 10 TN patients and 10 healthy individuals. Genotyping of 5-HTR2A rs6311 polymorphism using DNA isolated from blood, Real-Time PCR in StepOnePlus device, and Taqman SNP Genotyping Tests were performed by manufacturers' protocols.
Results: In 5HTR2A rs6311 analysis, 3 of 10 TN patients were found to have CC, 5 had CT and 2 had TT genotype. In the control group, CC genotype was detected in 4 individuals, CT genotype in 4 individuals, and TT genotype in 2 individuals.
Conclusion: It was found that the TN genotype and allelic frequency differences between the patient and control groups were not significant. However, the CT genotype was found to be more common.
Anahtar Kelimeler
Trigeminal neuralgia polymorphism serotonin serotonin receptor
Destekleyen Kurum
This work has been supported by Marmara University Scientific Research Projects Coordination Unit.
Proje Numarası
Grant number TYL-2022-10427
Kaynakça
- Berger M, Gray JA, Roth BL. The expanded biology of serotonin. Annual review of medicine, 2019;60:355-366.
- Cui W, Yu X, Zhang H. The serotonin transporter gene polymorphism is associated with the susceptibility and the pain severity in idiopathic trigeminal neuralgia patients. J. Headache. Pain. 2014;15(1):42.
- Di Stefano G, Yuan JH, Cruccu G, Waxman SG, Dib-Hajj SD, Truini A. Familial trigeminal neuralgia–a systematic clinical study with a genomic screen of the neuronal electrogenisome. Cephalalgia. 2020;40(8):767-777.
- Ebdrup BH, Rasmussen H, Arnt J, Glenthoj B. Serotonin 2A receptor antagonists for treatment of schizophrenia. Expert. Opin. Investig. 2011;20(9):1211-1223.
- Golimbet VE, Lavrushina OM, Kaleda VG, Abramova LI, Lezheiko TV. Supportive evidence for the association between the T102C 5-HTR2A gene polymorphism and schizophrenia: a large-scale case–control and family-based study. Eur. Psychiatry. 2007;22(3):167-170.
- Kazanci D, Polat T, Doğan CS, Aslan BT, Oktay Ş, Bilici MF, Kaynar Ö, Eken BF, Ulucan K. The determination of IL-6 rs1800795 polymorphism distribution in Turkish national cross-country skiing athletes sub-groups created referring to the 1km CCSTAs. Clin. Exp. Health Sci. 2021;11(4):782-786.
- Knezevic NN, Tverdohleb T, Knezevic I, Candido KD. The role of genetic polymorphisms in chronic pain patients. Int. J. Mol. Sci. 2018;19(6):1707.
- Küçükkurt S, Tükel HC, Özle M. Trigeminal nevralji. J. Dent. Fac. Ataturk. Univ. 2019;29(3): 501-511.
- Lee JY, Jeon BS, Kim HJ, Park SS. Genetic variant of HTR2A associates with risk of impulse control and repetitive behaviors in Parkinson’s disease. Park. Relat. Disord. 2012;18(1):76-78.
- Maarbjerg S, Di Stefano G, Cruccu G. Trigeminal neuralgia–diagnosis and treatment. Cephalalgia. 2017;37(7):648-657.
- McMillan R. Trigeminal Neuralgia—a debilitating facial pain. Rev. Pain. 2011; 5(1):26-34.
- Naito Y, Ishii M, Nagamine A, Imagawa A, Shida K, Takahashi J, Kiuchi Y. Association of the A-1438G polymorphism in serotonin 2A receptor in migraine with aura among Japanese patients. Biol. Pharm. Bull. 2010;33(10):1751-1753.
- Paredes S, Cantillo S, Candido KD, Knezevic NN. An association of serotonin with pain disorders and its modulation by estrogens. Int. J. Mol. Sci. 2019;20(22):5729.
- Regina ML, Airey DC, Manier DH, Shelton RC, Sanders-Bush E. Polymorphisms in the regulatory region of the human serotonin 5-HT2A receptor gene (HTR2A) influence gene expression. Biol. Psychiatry. 2007;61(2):167-173.
- Siqueira SRDT, Alves B, Malpartida HMG, Teixeira MJ, Siqueira JTT. Abnormal expression of voltage-gated sodium channels Nav1. 7, Nav1. 3 and Nav1. 8 in trigeminal neuralgia. Neurosci. 2009;164(2):573-577.
- Thome J, Retz W, Baader M, Pesold B, Hu M, Cowen M, Rösler M. Association analysis of HTR6 and HTR2A polymorphisms in sporadic Alzheimer's disease. J. Neural. Transm. 2001;108(10):1175-1180.
- Yücel Y, Coşkun S, Cengiz B, Özdemir HH, Uzar E, Çim A, Aluclu MU. Association of polymorphisms within the serotonin receptor genes 5-HTR1A, 5-HTR1B, 5-HTR2A and 5-HTR2C and migraine susceptibility in a Turkish population. Clin. Psychopharmacol. Neurosci. 2016;14(3):250.
Trigeminal Nevralji Hastalığında Serotonin 2A Reseptör Geni 5-HTR2A rs6311 Polimorfizminin Araştırılması
Öz
Amaç: Trigeminal nevralji (TN), trigeminal sinirin dağılım bölgesinde ortaya çıkan ve sıklıkla tekrarlayan ağrılarla karakterize bir hastalıktır. Serotonin (5-HT), merkezi sinir sistemindeki nörotransmitterlere etki eder. Çalışmada, TN'yi genetik olarak inceleyerek hastalarda serotonin reseptör geni (5-HTR2A) duyarlılığı ve ağrı şiddeti ile rs6311 polimorfizmi arasındaki ilişkiyi araştırmak amaçlanmıştır.
Gereç ve Yöntem: Çalışmada 10 TN hastası ve 10 sağlıklı birey yer almıştır. Kanlardan izole edilen DNA, StepOnePlus cihazında Real-Time PCR ve Taqman SNP Genotipleme Testleri kullanılarak 5-HTR2A rs6311 polimorfizminin genotiplenmesi üretici protokollerine göre yapıldı.
Bulgular: 5HTR2A rs6311 analizinde, 10 TN hastasından 3'ünde CC, 5'inde CT ve 2'sinde TT genotipinin varlığı tespit edildi. Kontrol grubunda ise 4 kişide CC genotipi, 4 kişide CT genotipi ve 2 kişide TT genotipi tespit edildi.
Sonuç: Hasta ve kontrol grupları arasındaki TN genotipi frekans farklılıklarının anlamlı olmadığı görüldü. Ancak CT genotipinin diğerlerine göre daha yaygın olduğu görüldü.
Anahtar Kelimeler
Trigeminal nevralji polimorfizm serotonin serotonin reseptörü
Proje Numarası
Grant number TYL-2022-10427
Kaynakça
- Berger M, Gray JA, Roth BL. The expanded biology of serotonin. Annual review of medicine, 2019;60:355-366.
- Cui W, Yu X, Zhang H. The serotonin transporter gene polymorphism is associated with the susceptibility and the pain severity in idiopathic trigeminal neuralgia patients. J. Headache. Pain. 2014;15(1):42.
- Di Stefano G, Yuan JH, Cruccu G, Waxman SG, Dib-Hajj SD, Truini A. Familial trigeminal neuralgia–a systematic clinical study with a genomic screen of the neuronal electrogenisome. Cephalalgia. 2020;40(8):767-777.
- Ebdrup BH, Rasmussen H, Arnt J, Glenthoj B. Serotonin 2A receptor antagonists for treatment of schizophrenia. Expert. Opin. Investig. 2011;20(9):1211-1223.
- Golimbet VE, Lavrushina OM, Kaleda VG, Abramova LI, Lezheiko TV. Supportive evidence for the association between the T102C 5-HTR2A gene polymorphism and schizophrenia: a large-scale case–control and family-based study. Eur. Psychiatry. 2007;22(3):167-170.
- Kazanci D, Polat T, Doğan CS, Aslan BT, Oktay Ş, Bilici MF, Kaynar Ö, Eken BF, Ulucan K. The determination of IL-6 rs1800795 polymorphism distribution in Turkish national cross-country skiing athletes sub-groups created referring to the 1km CCSTAs. Clin. Exp. Health Sci. 2021;11(4):782-786.
- Knezevic NN, Tverdohleb T, Knezevic I, Candido KD. The role of genetic polymorphisms in chronic pain patients. Int. J. Mol. Sci. 2018;19(6):1707.
- Küçükkurt S, Tükel HC, Özle M. Trigeminal nevralji. J. Dent. Fac. Ataturk. Univ. 2019;29(3): 501-511.
- Lee JY, Jeon BS, Kim HJ, Park SS. Genetic variant of HTR2A associates with risk of impulse control and repetitive behaviors in Parkinson’s disease. Park. Relat. Disord. 2012;18(1):76-78.
- Maarbjerg S, Di Stefano G, Cruccu G. Trigeminal neuralgia–diagnosis and treatment. Cephalalgia. 2017;37(7):648-657.
- McMillan R. Trigeminal Neuralgia—a debilitating facial pain. Rev. Pain. 2011; 5(1):26-34.
- Naito Y, Ishii M, Nagamine A, Imagawa A, Shida K, Takahashi J, Kiuchi Y. Association of the A-1438G polymorphism in serotonin 2A receptor in migraine with aura among Japanese patients. Biol. Pharm. Bull. 2010;33(10):1751-1753.
- Paredes S, Cantillo S, Candido KD, Knezevic NN. An association of serotonin with pain disorders and its modulation by estrogens. Int. J. Mol. Sci. 2019;20(22):5729.
- Regina ML, Airey DC, Manier DH, Shelton RC, Sanders-Bush E. Polymorphisms in the regulatory region of the human serotonin 5-HT2A receptor gene (HTR2A) influence gene expression. Biol. Psychiatry. 2007;61(2):167-173.
- Siqueira SRDT, Alves B, Malpartida HMG, Teixeira MJ, Siqueira JTT. Abnormal expression of voltage-gated sodium channels Nav1. 7, Nav1. 3 and Nav1. 8 in trigeminal neuralgia. Neurosci. 2009;164(2):573-577.
- Thome J, Retz W, Baader M, Pesold B, Hu M, Cowen M, Rösler M. Association analysis of HTR6 and HTR2A polymorphisms in sporadic Alzheimer's disease. J. Neural. Transm. 2001;108(10):1175-1180.
- Yücel Y, Coşkun S, Cengiz B, Özdemir HH, Uzar E, Çim A, Aluclu MU. Association of polymorphisms within the serotonin receptor genes 5-HTR1A, 5-HTR1B, 5-HTR2A and 5-HTR2C and migraine susceptibility in a Turkish population. Clin. Psychopharmacol. Neurosci. 2016;14(3):250.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Diş Hekimliği (Diğer) |
| Bölüm | Özgün Araştırmalar |
| Yazarlar | |
| Proje Numarası | Grant number TYL-2022-10427 |
| Erken Görünüm Tarihi | 29 Nisan 2024 |
| Yayımlanma Tarihi | 30 Nisan 2024 |
| Gönderilme Tarihi | 17 Mart 2024 |
| Kabul Tarihi | 19 Nisan 2024 |
| Yayımlandığı Sayı | Yıl 2024 Cilt: 8 Sayı: 1 |
