Investigation of the Clinical Effectiveness of Ozone Therapy on Experimental Acidic Skin Burns in Rats
Öz
This study aims to research the clinical efficacy of ozone therapy on experimentally induced hydrofluoric (HFA) acidic skin burns in rats. The study material consists of 20 healthy male rats weighing 200-250 g. The animals were grouped as experimental and control group, with 10 animals in each. Acidic skin burn was created with 38% HFA under general anaesthesia in all groups. Ozonated liquid Vaseline was applied daily to the wound area in experimental group for 7 days and 0.9% saline was applied daily to the control group for 7 days. In the study, bullae, erythema, ne- crosis, healing, hair growth and damaged area data were clinically evaluated and statistically compared. As a result of the statistical analysis, there was no significant difference determined between the experimental and control group in clinical examination but there was a significant difference found in hair growth (P<0.05). As a result of this study; It was determined that the number of animals which recovered in the group treated with ozone treatment in experimentally induced acidic skin burns was higher and the recovery was statistically significant in the 8thday experimental group. It was concluded that ozone may be more favourable than hydrotherapy in emergency interventions in acidic skin burns, However, more detailed research on the subject is needed.
Anahtar Kelimeler
Kaynakça
- Abazari M, Ghaffari A, Rashidzadeh H, Badeleh SM, Maleki Y. A Systematic review on classification, identification and healing process of burn wound healing. Int J Low Extrem Wounds 2020; 21(1): 18-30.
- Altan S, Oğurtan Z. Dimethyl sulfoxide but not indo- methacin is efficient for healing in hydrofluoric acid eye burns. Burns 2017; 43(1): 232-44.
- Altan S, Sağsöz H, Oğurtan Z. Topical dimethyl sulfoxide inhibits corneal neovacularization and stimulates corneal repair in rabbits following acid burn. Biotec Histochem 2017; 92(8): 619-36.
- Broughton G, Janis JE, Attinger CE. The basic science of wound healing. Plast Reconst Surg 2006; 117(7): 12-34.
- Burgher F, Mathieu L, Lati E, Gasser P, Peno- Mazzarino L, Blornet J, Hall AH, Maibach HI. Experimental 70% hydrofluoric acid burns: histo- logical observations in an established human skin explants ex vivo model. Cutan Ocul Toxicol 2011; 30(2): 100-7.
- Campanati A, De Blasio S, Giuliano A, Ganzetti G, Giuliodori K, Pecora T, Consales V, Minnetti I, Offidani A. Topical ozonated oil versus hyaluronic gel for the treatment of partial to full thickness second degree burns: A prospective, comparative, single-blind, non-randomised, controlled clinical trial. Burns 2013; 39(6): 1178-83.
- Canedo-Dorantes L, Canedo-Ayala M. Skin acute wound healing: A compherensive review. Int J Inflam 2019; 19(1): 1-15.
- Degli Agosti I, Ginelli E, Mazzacane B, Peroni G, Bianco S, Guerriero F, Ricevuti G, Perna S, Ron- danelli M. Effectiveness of a short-term treatment of oxygen-ozone therapy into healing in a posttraumatic wound. Case Rep Med 2016; 16(1): 1-6.
- Fitzpatrick E, Holland OJ, Vanderlelie JJ. Ozone therapy for the treatment of chronic wounds: A systematic review. Int Wound J 2018; 15(4): 633-44.
- Gantwerker EA, Hom DB. Skin: Histology and physio- logy of wound healing. Clin Plast Surg 2012; 39 (1): 85-97.
- Han HH, Kwon BY, Jung SN, Moon SH. Importance of initial management and surgical treatment after hydrofluoric acid burn of the finger. Burns 2017; 43(1): 1-6.
- Hoffmann S, Parikh P, Bohnenberger K. Dermal hydrofluoric acid toxicity case review: Looks can be deceiving. J Emerg Nurs 2021; 47(1); 28-32.
- Kodik MS. Deneysel hidroflorik asit deri yanıklarında acil uygulanan tedavi metodlarının iyileşme üzeri- ne etkilerinin karşılaştırılması, Uzmanlık tezi, Ege Üniv. Tıp Fak. Acil Tıp ABD, İzmir 2011; ss.1-72.
- McKee D, Thoma A, Bailey K, Fish J. A review of hydrofluoric acid burn management. Can J Plast Surg 2014; 22(2): 95-8.
- Mohammed Al-Dalain S, Martinez G, Candelario-Jalil E, Menendez S, Re L, Giuliani A, Sonia Leon O. Ozone treatment reduces markers of oxidative and endothelial damage in an experimental diabetes model in rats. Pharmacol Res 2001; 44(5): 391-6.
- Özcan M, Allahbeickaraghi A, Dündar M. Possible hazardous effects of hydrofluoric acid and recommendations for treatment approach: A review. Clin Oral Investig 2012; 16(1): 15-23.
- Pivotto AP, Banhuk FW, Staffen IV, Daga MA, Ayala TS, Menolli RA. Clinical uses and molecular aspects of ozone therapy: A review. Online J Biol Sci 2020; 20(1): 37-49.
- Rippa AL, Kalabusheva EP, Vorotelyak EA. Regene- ration of dermis: Scarring and cells involved. Cells 2019; 8(6): 607.
- Robinson EP, Chhabra AB. Hand chemical burns. J Hand Surg 2015; 40(3); 605-12.
- Roblin I, Urban M, Flicoteau D, Martin C, Pradeu D. Topical treatment of experimental hydrofluoric acid skin burns by 2.5% calcium gluconate. J Burn Care Res 2006; 27(6): 889-94.
- Roosterman D, Goerge T, Schneider SW, Bunnett NW, Steinhoff M. Neuronal control of skin function: The skin as a neuroimmunoendocrine organ. Physiol Rev 2006; 86(4): 1309-79.
- Rowan MP, Cancio LC, Elster EA, Burmeister DM, Rose LF, Natesan S, Chan RK, Christy RJ, Chung KK. Burn wound healing and treatment: Review and advancements. Crit Care 2015; 19 (1); 1-12.
- Sciorsci RL, Lillo E, Occhiogrosso L, Rizzo A. Ozone therapy in veterinary medicine: A review. Res Vet Sci 2020; 130(1): 240-6.
- Travagli V, Zanardi I, Valacchi G, Bocci V. Ozone and ozonated oils in skin diseases: A review. Mediators Inflam 2010; 10(1): 1-10.
- Valacchi G, Fortino V, Bocci V. The dual action of ozone on the skin. Br J Dermatol Suppl 2005; 153 (6): 1096-100.
- Williams FN, Lee JO. Chemical burns. In: Total Burn Care. New York: Elsevier Inc, 2018; pp. 408-13.
- Wu ML, Deng JF, Fan JS. Survival after hypocalcemia, hypomagnesemia, hypokalemia and cardiac arrest following mild hydrofluoric acid burn. Clin Toxicol 2010; 48(9): 953-5.
- Xingang W, Yuanhai Z, Liangfang N, Chuangang Y, Chunjiang Y, Ruiming J, Liping L, Jia L, Chunmao H. A review of treatment strategies for hydrofluoric acid burns: Current status and future prospects. Burns 2014; 40(8): 1447-57.
- Yamanel L, Kaldirim U, Oztas Y, Coskun O, Poyrazoglu Y, Durusu M, Cayci T, Ozturk A, Demirbas S,Yasar M, Cinar O, Tuncer SK, Eyi YE, Uysal B, Topal T, Oter S, Korkmaz A. Ozone therapy and hyperbaric oxygen treatment in lung injury in septic rats. Int J Med Sci 2011; 8(1): 48-55.
- Yaşar Z. Deneysel haşlanma yanık modelinde hiper- barik oksijen tedavisi ve medikal ozon tedavisinin yara iyileşmesinde etkilerinin karşılaştırılması. Uzmanlık tezi, Dicle Üniv Tıp Fak, Diyarbakır 2011; s.1-112.
- Zeng J, Lu J. Mechanisms of action involved in ozone -therapy in skin diseases. Int Immunopharmacol 2018; 56(1): 235-41.
Ratlarda Deneysel Olarak Oluşturulan Asidik Deri Yanıklarında Uygulanan Ozon Tedavisinin Klinik Etkinliğinin Araştırılması
Öz
Bu çalışmada ratlarda hidroflorik asit (HFA) ile deneysel olarak oluşturulan asidik deri yanıklarında ozon tedavisinin klinik etkinliğinin araştırılması amaçlanmıştır. Çalışma materyalini 20 adet, sağlıklı erkek, 200-250 gr ağırlığındaki ratlar oluşturdu. Çalışmaya dahil edilen hayvanlar her bir grupta 10 adet hayvan olacak şekilde deney ve kontrol grubu olarak gruplandırıldı. Grupları oluşturan tüm hayvanlarda genel anestezi altında %38’lik HFA ile asidik deri yanığı oluşturuldu. Deri yanığı oluşturulan çalışma grubundaki tüm hayvanların yara bölgesine 7 gün boyunca günde bir kere ozonlanmış sıvı vazelin, kontrol grubundaki tüm hayvanlara ise %0.9’luk serum fizyolojik 7 gün süre ile günde bir kere uygulandı. Çalışmada klinik olarak değerlendirmeye alınan bül, eritem, nekroz, iyileşme, tüylenme ve oluşan hasarlı alan verileri- nin istatistiksel analizleri karşılaştırıldı. İstatistiksel analiz sonucunda deney ve kontrol grupları arasında bül, eritem, nekroz, iyileşme ve oluşan hasarlı alan bakımından anlamlı farklılık olmadığı, ancak tüylenmede 5. günden itibaren anlamlı farklılık olduğu tespit edildi (P<0.05). Bu çalışma sonucunda; deneysel olarak oluşturulan asidik deri yanıkların- da ozon tedavisi yapılan grupta iyileşen hayvan sayısının daha fazla olduğu ve iyileşmenin 8. gün deney grubunda istatistiksel olarak anlamlılık seviyesinde olduğu tespit edildi. Ozonun asidik deri yanıklarında acil müdahale olarak uygulanan hidroterapiye göre daha iyi olabileceği fakat konuyla ilgili daha detaylı araştırmaların yapılması gerektiği kanısına varıldı.
Anahtar Kelimeler
Deri hidroflorik asit ozon yanık yara Burn hydrofluoric acid ozone skin wound
Kaynakça
- Abazari M, Ghaffari A, Rashidzadeh H, Badeleh SM, Maleki Y. A Systematic review on classification, identification and healing process of burn wound healing. Int J Low Extrem Wounds 2020; 21(1): 18-30.
- Altan S, Oğurtan Z. Dimethyl sulfoxide but not indo- methacin is efficient for healing in hydrofluoric acid eye burns. Burns 2017; 43(1): 232-44.
- Altan S, Sağsöz H, Oğurtan Z. Topical dimethyl sulfoxide inhibits corneal neovacularization and stimulates corneal repair in rabbits following acid burn. Biotec Histochem 2017; 92(8): 619-36.
- Broughton G, Janis JE, Attinger CE. The basic science of wound healing. Plast Reconst Surg 2006; 117(7): 12-34.
- Burgher F, Mathieu L, Lati E, Gasser P, Peno- Mazzarino L, Blornet J, Hall AH, Maibach HI. Experimental 70% hydrofluoric acid burns: histo- logical observations in an established human skin explants ex vivo model. Cutan Ocul Toxicol 2011; 30(2): 100-7.
- Campanati A, De Blasio S, Giuliano A, Ganzetti G, Giuliodori K, Pecora T, Consales V, Minnetti I, Offidani A. Topical ozonated oil versus hyaluronic gel for the treatment of partial to full thickness second degree burns: A prospective, comparative, single-blind, non-randomised, controlled clinical trial. Burns 2013; 39(6): 1178-83.
- Canedo-Dorantes L, Canedo-Ayala M. Skin acute wound healing: A compherensive review. Int J Inflam 2019; 19(1): 1-15.
- Degli Agosti I, Ginelli E, Mazzacane B, Peroni G, Bianco S, Guerriero F, Ricevuti G, Perna S, Ron- danelli M. Effectiveness of a short-term treatment of oxygen-ozone therapy into healing in a posttraumatic wound. Case Rep Med 2016; 16(1): 1-6.
- Fitzpatrick E, Holland OJ, Vanderlelie JJ. Ozone therapy for the treatment of chronic wounds: A systematic review. Int Wound J 2018; 15(4): 633-44.
- Gantwerker EA, Hom DB. Skin: Histology and physio- logy of wound healing. Clin Plast Surg 2012; 39 (1): 85-97.
- Han HH, Kwon BY, Jung SN, Moon SH. Importance of initial management and surgical treatment after hydrofluoric acid burn of the finger. Burns 2017; 43(1): 1-6.
- Hoffmann S, Parikh P, Bohnenberger K. Dermal hydrofluoric acid toxicity case review: Looks can be deceiving. J Emerg Nurs 2021; 47(1); 28-32.
- Kodik MS. Deneysel hidroflorik asit deri yanıklarında acil uygulanan tedavi metodlarının iyileşme üzeri- ne etkilerinin karşılaştırılması, Uzmanlık tezi, Ege Üniv. Tıp Fak. Acil Tıp ABD, İzmir 2011; ss.1-72.
- McKee D, Thoma A, Bailey K, Fish J. A review of hydrofluoric acid burn management. Can J Plast Surg 2014; 22(2): 95-8.
- Mohammed Al-Dalain S, Martinez G, Candelario-Jalil E, Menendez S, Re L, Giuliani A, Sonia Leon O. Ozone treatment reduces markers of oxidative and endothelial damage in an experimental diabetes model in rats. Pharmacol Res 2001; 44(5): 391-6.
- Özcan M, Allahbeickaraghi A, Dündar M. Possible hazardous effects of hydrofluoric acid and recommendations for treatment approach: A review. Clin Oral Investig 2012; 16(1): 15-23.
- Pivotto AP, Banhuk FW, Staffen IV, Daga MA, Ayala TS, Menolli RA. Clinical uses and molecular aspects of ozone therapy: A review. Online J Biol Sci 2020; 20(1): 37-49.
- Rippa AL, Kalabusheva EP, Vorotelyak EA. Regene- ration of dermis: Scarring and cells involved. Cells 2019; 8(6): 607.
- Robinson EP, Chhabra AB. Hand chemical burns. J Hand Surg 2015; 40(3); 605-12.
- Roblin I, Urban M, Flicoteau D, Martin C, Pradeu D. Topical treatment of experimental hydrofluoric acid skin burns by 2.5% calcium gluconate. J Burn Care Res 2006; 27(6): 889-94.
- Roosterman D, Goerge T, Schneider SW, Bunnett NW, Steinhoff M. Neuronal control of skin function: The skin as a neuroimmunoendocrine organ. Physiol Rev 2006; 86(4): 1309-79.
- Rowan MP, Cancio LC, Elster EA, Burmeister DM, Rose LF, Natesan S, Chan RK, Christy RJ, Chung KK. Burn wound healing and treatment: Review and advancements. Crit Care 2015; 19 (1); 1-12.
- Sciorsci RL, Lillo E, Occhiogrosso L, Rizzo A. Ozone therapy in veterinary medicine: A review. Res Vet Sci 2020; 130(1): 240-6.
- Travagli V, Zanardi I, Valacchi G, Bocci V. Ozone and ozonated oils in skin diseases: A review. Mediators Inflam 2010; 10(1): 1-10.
- Valacchi G, Fortino V, Bocci V. The dual action of ozone on the skin. Br J Dermatol Suppl 2005; 153 (6): 1096-100.
- Williams FN, Lee JO. Chemical burns. In: Total Burn Care. New York: Elsevier Inc, 2018; pp. 408-13.
- Wu ML, Deng JF, Fan JS. Survival after hypocalcemia, hypomagnesemia, hypokalemia and cardiac arrest following mild hydrofluoric acid burn. Clin Toxicol 2010; 48(9): 953-5.
- Xingang W, Yuanhai Z, Liangfang N, Chuangang Y, Chunjiang Y, Ruiming J, Liping L, Jia L, Chunmao H. A review of treatment strategies for hydrofluoric acid burns: Current status and future prospects. Burns 2014; 40(8): 1447-57.
- Yamanel L, Kaldirim U, Oztas Y, Coskun O, Poyrazoglu Y, Durusu M, Cayci T, Ozturk A, Demirbas S,Yasar M, Cinar O, Tuncer SK, Eyi YE, Uysal B, Topal T, Oter S, Korkmaz A. Ozone therapy and hyperbaric oxygen treatment in lung injury in septic rats. Int J Med Sci 2011; 8(1): 48-55.
- Yaşar Z. Deneysel haşlanma yanık modelinde hiper- barik oksijen tedavisi ve medikal ozon tedavisinin yara iyileşmesinde etkilerinin karşılaştırılması. Uzmanlık tezi, Dicle Üniv Tıp Fak, Diyarbakır 2011; s.1-112.
- Zeng J, Lu J. Mechanisms of action involved in ozone -therapy in skin diseases. Int Immunopharmacol 2018; 56(1): 235-41.
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Bölüm | Araştırma Makalesi |
| Yazarlar | |
| Yayımlanma Tarihi | 1 Aralık 2022 |
| Gönderilme Tarihi | 20 Mayıs 2022 |
| Kabul Tarihi | 15 Ağustos 2022 |
| Yayımlandığı Sayı | Yıl 2022 Cilt: 19 Sayı: 3 |
Kaynak Göster
https://dergipark.org.tr/tr/download/journal-file/20610
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