Nitrotyrosine formation, iNOS and the Na+,K+-ATPase activities in sepsis: The possible effects of CAPE

Leyla Çimen; Behzat Çimen; Aysun Çetin

Araştırma Makalesi

Yıl 2020, Cilt: 1 Sayı: 2, 52 - 59, 29.09.2020

Leyla Çimen Behzat Çimen Aysun Çetin

Öz

Destekleyen Kurum

Erciyes Üniversitesi BAP

Proje Numarası

TDK-2016-6596

Kaynakça

1. Seven I, Türközkan N and Cimen B. The effects of nitric oxide synthesis on the Na+,K+-ATPase activity in guinea pig kidney exposed to lipopolysaccharides. Molecular and Cellular Biochemistry 2005; 271: 107–112
2. Olsson LE, Wheeler MA, Sessa WC, et al. Bladder instillation and intraperitoneal injection of Escherichia coli lipopolysaccharide up-regulate cytokines and iNOS in rat ürinary bladder. J Pharm Exp Ther 1998; 284:1203-1208
3. Schwartz D, Mendonca M, Schwartz I, et al. Inhibition of constitutive nitric oxide synthase (NOS) by nitric oxide generated by inducible NOS after lipopolysaccharide administration provokes renal dysfunction in rats. J Clin Invest 1997; 100:439-448
4. Markewitz BA, Michael JR, Kohan DE. Cytokine-induced expression of a nitric oxide synthase in rat renal tubule cells. J Clin Invest 1993; 91:2138-2143
5. Zhang C, Walker LM, Mayeux PR. Role of nitric oxide in lipopolysaccharide-induced oxidant stres in the rat kidney. Biochem Pharmacol 2000; 59:203-209
6. Fukuyama N, Takebayashi Y, Hida M, et al. Clinical evidence of peroxynitrite formation in chronic renal failure patients with septic shock. Free radic biol med 1997;22:771-774
7. Bian K, Davis K, Kuret J, et al. Nitrotyrosine formation with endotoxin-induced kidney injury detected by immunohistochemistry. Am J Physiol 1999; 277:F33-40
8. Mishra OP, Delivoria-Papadopoulos M, Cahilane G, et al. Lipid peroxidation as the mechanism of modification of the affinity of the Na+,K+-ATPase active sites for ATP, K+,Na+, and strophanthidin in vitro. Neurochem Res 1989; 14:845-851
9. Sato T, Kamata Y, Irifune M, et al. Inhibitory effect of several nitric oxide-generating compounds on purified Na+,K+-ATPase activity from porcine cerebral cortex. J Neurochem 1997; 68:1312-1318
10. Qayyum I, Zubrow AB, Ashraf QM, et al. Nitration as a mechanism of Na+,K+-ATPase modification during hypoxia in the cerebral cortex of the guinea pig fetus. Neurochem Res 2001; 26:1163-1169
11. Türközkan N, Ünlü A, Ertabak A, et al. The effects of peroxynitrite on erythrocytes. Clin Chem Lab Med 2001; 39:1263-1266
12. Zhang C, Imam SZ, Ali SF, et al. Peroxynitrite and the regulation of Na+,K+-ATPase activity by angiotensin II in the rat proximal tubule. Nitric Oxide 2002; 7:30-35
13. Muriel P, Sandoval G. Nitric oxide and peroxynitrite anion modulate liver plasma membrane fluidity and Na+,K+-ATPase activity. Nitric Oxide 2000; 4:333-342
14. Kang DG, Kim JW, Lee J. Effects of nitric oxide synthesis inhibition on the Na,K-ATPase activity in the kidney. Pharm Res 2000; 41:121-125
15. Guzman NJ, Fang MZ, Tang SS, et al. Autocrine inhibition of Na+K+ATPase by nitric oxide in mouse proximal tubule epithelial cells. J Clin Invest 1995; 95:2083-2088 16. Parlakpınar H, Örüm MH, Acet A. Kafeik asit fenetil ester (KAFE) ve miyokardiyal iskemi reperfüzyon (Mİ/R) hasarı. İnönü Üniversitesi Sağlık Bilimleri Dergisi 2012; 1: 10-5
17. Ueki M, Taie S, Chujo K. et al. Urinary trypsin inhibitor reduces inflammatory response in kidney induced by LPS. J Bioscience and Bioengineering. 2007; 104(4): 315-320
18. Akyol S, Akbas A, Butun I, et al. Caffeic acid phenethyl ester as a remedial agent for reproductive functions and oxidative stress-based pathologies of gonads. Journal of Intercultural Ethnopharmacology 2015; 4(2): 187-191
19. Tolba MF, Omar HA, Azab SS, et al. Caffeic acid phenethyl ester: A review of its antioxidant activity, protective effects against ischemia-reperfusion injury and drug adverse reactions. Critical Reviews in Food Science and Nutrition 2016; 56: 2183-2190
20. Yılmaz HR, Sögüt S, Özyurt H, et al. Sıçanlarda sispilatinle oluşturulan nefrotoksisitede metabolik enzim aktivitelerine kafeik asit fenetil ester’in etkisi. Van Tıp Dergisi 2004; 11(1): 1-6
21. Koksel O, Ozdulger A, Tamer L, et al. Effects of caffeic acid phenethyl ester on lipopolysaccharide-induced lung injury in rats. Pulmonary Pharmacology & Therapeutics 2006; 19: 90-95
22. Alici O, Kavakli HS, Koca C, et al. Value of caffeic acid phenethyl ester pretreatment in experimental sepsis model in rats. Mediators of Inflammation 2015; 2015: 1-6
23. Erdoğan O, Tüz M, Yasan H, et al. Deneysel akustik travmada kafeik asit fenetil esterin işitme kaybı üzerine etkisi. Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi 2012; 19(3): 81-86
24. Motawi TK, Darwish HA, Abd El Tawab AM. Effects of caffeic acid phenethyl ester on endotoxin-induced cardiac stress in rats: A Possible mechanism of protection. J Biochem Molecular Toxicology 2011; 25(2): 84-94
25. Çimen B, Türközkan N, Seven I, et al. Impaired Na-K-ATPase activity as a mechanism of reactive nitrogen species induced cytotoxicity in guinea pig liver exposed to lipopolysaccharides. Molecular and Cellular Biochemistry 2004; 259(1-2): 53-57
26. Türközkan N, Seven I, Erdamar H, et al. Effect of Vitamin A pretreatment on Escherichia coli-induced lipid peroxidation and level of 3-Nitrotyrosine in kidney of guinea pig. Molecular and Cellular Biochemistry 2005; 278(1-2): 33-37
27. Unlu A, Türközkan N, Cimen B, et al. The effect of E.coli-derived lipopolysaccharides on plasma levels of malondialdehyde and 3-nitrotyrosine. Clin Chem Lab Med. 2001; 39:491-493 28. Ertabak A, Kutluay T, Unlu A, et al. The effects of desferrioxamine on peroxynitrite-induced oxidative damage in erythrocytes. Cell Biochem Funct 2004; 22: 149–152
29. Serpersu E, Ciliv G. Some properties of (Na+K+)-dependent adenosinetriphosphatase from human erythrocytes. Biochem Med. 1978; 20: 31-39 30. Feelisch M., Noack E.A. Correlation between Nitric Oxide Formation during Degradation of Organic Nitrates and Activation of Guanylate Cyclase, Eur J Pharmacol 1987; 139:19-30
31. Knowles RG, Marrett M, Salter M, et al. Differential induction of brain, lung and liver nitric oxide synthase by endotoxin in the rat. Biochem J 1990; 270:833-836 32. Liu S, Adcock IM, Old RW, et al. Lipopolysaccharide treatment in vivo induces widespread tissue expression of inducible nitric oxide synthase mRNA. Biochem Biophys Res Commun 1993; 196:1208-1213
33. Yang F, Comtois AS, Fang L, et al. Nitric oxide derived-nitrate anion contributes to endotoxic shock and multiple organ injury/dysfunction. Crit Care Med 2002; 30: 650-657
34. Paya D, Stoclet JC. Involvement of bradykinin and nitric oxide in the early hemodynamic effects of lipopolysaccharide in rats. Shock 1995; 3:376-379
35. Morrissey JJ, McCracken R, Kaneto H, et al. Location of an inducible nitric oxide synthase mRNA in the normal kidney. Kidney Int 1994; 45:998-1005
36. Mayeux PR, Garner HR, Gibson JD, et al. Effect of lipopolysaccharide on nitric oxide synthase activity in rat proximal tubules. Biochem Pharm 1995; 49:115-118
37. D’Ambrossio SM, Gibson-D’Ambrossio RE, Brady T, et al. Mechanisms of nitric oxide-induced cytotoxicity in normal human hepatocytes. Environ Mol Mutagen 2001; 37:46-54
38. Song YS, Park EH, Hur GM, et al. Caffeic acid phenethyl ester inhibits nitric oxide synthase gene expression and enzyme activity. Cancer Lett 2002;175(1):53-61
39. Celik S, Erdogan S. Caffeic acid phenethyl ester (CAPE) protects brain against oxidative stress and inflammation induced by diabetes in rats. Mol Cell Biochem 2008; 312(1-2):39-46
40. Kassim M, Mansor M, Kamalden TA, et al. Caffeic acid phenethyl ester (CAPE): scavenger of peroxynitrite in vitro and in sepsis models. Shock 2014; 42(2):154-60
41. Çakır T, Özkan E, Dulundu E, et al. Caffeic acid phenethyl ester (CAPE) prevents methotrexate-induced hepatorenal oxidative injury in rats. J Pharm Pharmacol 2011; 63(12):1566-71

Nitrotyrosine formation, iNOS and the Na+,K+-ATPase activities in sepsis: The possible effects of CAPE

Yıl 2020, Cilt: 1 Sayı: 2, 52 - 59, 29.09.2020

Leyla Çimen Behzat Çimen Aysun Çetin

Öz

Sepsis is a response to infection characterized by the formation of highly reactive oxygen and nitrogen substances. The rat kidney was chosen for this purpose because many important inflammatory mediators, including inducible nitric oxide synthase (iNOS) and nitrotyrosine (nTyr) production, are expressed by kidney cells following either lipopolysaccharide (LPS) or bacterial challenge.
The present study was aimed at investigating the relationship between nTyr formation with iNOS and Na+,K+-ATPase activities. We were also aimed at investigating the possible role of caffeic acid phenethyl ester (CAPE) on endogenous nTyr production, Na+,K+-ATPase and iNOS activities in the kidney.
In this study, kidney Na+,K+-ATPase activity were maximally inhibited 6h after LPS injection and LPS treatment significantly increased iNOS activity of kidney. The regression analysis negative correlation between Na+,K+-ATPase activity and nTyr levels of LPS treated animals. Na+,K+-ATPase activity were also negatively correlated with iNOS activity in LPS-treated rats. These data suggest that nitric oxide (NO.) and peroxynitrite (ONOO-) contribute to the development of oxidant injury. Furthermore, the source of NO. may be iNOS. iNOS are expressed by the kindey, and their activity may increase following LPS administration. In addition, NO. and ONOO- formation inhibited Na+,K+-ATPase activity. This results also have strongly suggested that bacterial LPS disturbs activity of membran Na+,K+-ATPase that may be an important component leading to the pathological consequences such as renal dysfunction in which the production of reactive nitrogen substance (RNS) are increased as in the case of LPS challenge. CAPE treatment was decreased nTyr production and iNOS activites, was increased Na+,K+-ATPase activity. These data suggest that CAPE treatment contribute to the decrease of oxidant injury.

Anahtar Kelimeler

Nitrotyrosine, Na+,K+-ATPase, iNOS, kidney, rat, LPS, CAPE

Proje Numarası

TDK-2016-6596

Kaynakça

1. Seven I, Türközkan N and Cimen B. The effects of nitric oxide synthesis on the Na+,K+-ATPase activity in guinea pig kidney exposed to lipopolysaccharides. Molecular and Cellular Biochemistry 2005; 271: 107–112
2. Olsson LE, Wheeler MA, Sessa WC, et al. Bladder instillation and intraperitoneal injection of Escherichia coli lipopolysaccharide up-regulate cytokines and iNOS in rat ürinary bladder. J Pharm Exp Ther 1998; 284:1203-1208
3. Schwartz D, Mendonca M, Schwartz I, et al. Inhibition of constitutive nitric oxide synthase (NOS) by nitric oxide generated by inducible NOS after lipopolysaccharide administration provokes renal dysfunction in rats. J Clin Invest 1997; 100:439-448
4. Markewitz BA, Michael JR, Kohan DE. Cytokine-induced expression of a nitric oxide synthase in rat renal tubule cells. J Clin Invest 1993; 91:2138-2143
5. Zhang C, Walker LM, Mayeux PR. Role of nitric oxide in lipopolysaccharide-induced oxidant stres in the rat kidney. Biochem Pharmacol 2000; 59:203-209
6. Fukuyama N, Takebayashi Y, Hida M, et al. Clinical evidence of peroxynitrite formation in chronic renal failure patients with septic shock. Free radic biol med 1997;22:771-774
7. Bian K, Davis K, Kuret J, et al. Nitrotyrosine formation with endotoxin-induced kidney injury detected by immunohistochemistry. Am J Physiol 1999; 277:F33-40
8. Mishra OP, Delivoria-Papadopoulos M, Cahilane G, et al. Lipid peroxidation as the mechanism of modification of the affinity of the Na+,K+-ATPase active sites for ATP, K+,Na+, and strophanthidin in vitro. Neurochem Res 1989; 14:845-851
9. Sato T, Kamata Y, Irifune M, et al. Inhibitory effect of several nitric oxide-generating compounds on purified Na+,K+-ATPase activity from porcine cerebral cortex. J Neurochem 1997; 68:1312-1318
10. Qayyum I, Zubrow AB, Ashraf QM, et al. Nitration as a mechanism of Na+,K+-ATPase modification during hypoxia in the cerebral cortex of the guinea pig fetus. Neurochem Res 2001; 26:1163-1169
11. Türközkan N, Ünlü A, Ertabak A, et al. The effects of peroxynitrite on erythrocytes. Clin Chem Lab Med 2001; 39:1263-1266
12. Zhang C, Imam SZ, Ali SF, et al. Peroxynitrite and the regulation of Na+,K+-ATPase activity by angiotensin II in the rat proximal tubule. Nitric Oxide 2002; 7:30-35
13. Muriel P, Sandoval G. Nitric oxide and peroxynitrite anion modulate liver plasma membrane fluidity and Na+,K+-ATPase activity. Nitric Oxide 2000; 4:333-342
14. Kang DG, Kim JW, Lee J. Effects of nitric oxide synthesis inhibition on the Na,K-ATPase activity in the kidney. Pharm Res 2000; 41:121-125
15. Guzman NJ, Fang MZ, Tang SS, et al. Autocrine inhibition of Na+K+ATPase by nitric oxide in mouse proximal tubule epithelial cells. J Clin Invest 1995; 95:2083-2088 16. Parlakpınar H, Örüm MH, Acet A. Kafeik asit fenetil ester (KAFE) ve miyokardiyal iskemi reperfüzyon (Mİ/R) hasarı. İnönü Üniversitesi Sağlık Bilimleri Dergisi 2012; 1: 10-5
17. Ueki M, Taie S, Chujo K. et al. Urinary trypsin inhibitor reduces inflammatory response in kidney induced by LPS. J Bioscience and Bioengineering. 2007; 104(4): 315-320
18. Akyol S, Akbas A, Butun I, et al. Caffeic acid phenethyl ester as a remedial agent for reproductive functions and oxidative stress-based pathologies of gonads. Journal of Intercultural Ethnopharmacology 2015; 4(2): 187-191
19. Tolba MF, Omar HA, Azab SS, et al. Caffeic acid phenethyl ester: A review of its antioxidant activity, protective effects against ischemia-reperfusion injury and drug adverse reactions. Critical Reviews in Food Science and Nutrition 2016; 56: 2183-2190
20. Yılmaz HR, Sögüt S, Özyurt H, et al. Sıçanlarda sispilatinle oluşturulan nefrotoksisitede metabolik enzim aktivitelerine kafeik asit fenetil ester’in etkisi. Van Tıp Dergisi 2004; 11(1): 1-6
21. Koksel O, Ozdulger A, Tamer L, et al. Effects of caffeic acid phenethyl ester on lipopolysaccharide-induced lung injury in rats. Pulmonary Pharmacology & Therapeutics 2006; 19: 90-95
22. Alici O, Kavakli HS, Koca C, et al. Value of caffeic acid phenethyl ester pretreatment in experimental sepsis model in rats. Mediators of Inflammation 2015; 2015: 1-6
23. Erdoğan O, Tüz M, Yasan H, et al. Deneysel akustik travmada kafeik asit fenetil esterin işitme kaybı üzerine etkisi. Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi 2012; 19(3): 81-86
24. Motawi TK, Darwish HA, Abd El Tawab AM. Effects of caffeic acid phenethyl ester on endotoxin-induced cardiac stress in rats: A Possible mechanism of protection. J Biochem Molecular Toxicology 2011; 25(2): 84-94
25. Çimen B, Türközkan N, Seven I, et al. Impaired Na-K-ATPase activity as a mechanism of reactive nitrogen species induced cytotoxicity in guinea pig liver exposed to lipopolysaccharides. Molecular and Cellular Biochemistry 2004; 259(1-2): 53-57
26. Türközkan N, Seven I, Erdamar H, et al. Effect of Vitamin A pretreatment on Escherichia coli-induced lipid peroxidation and level of 3-Nitrotyrosine in kidney of guinea pig. Molecular and Cellular Biochemistry 2005; 278(1-2): 33-37
27. Unlu A, Türközkan N, Cimen B, et al. The effect of E.coli-derived lipopolysaccharides on plasma levels of malondialdehyde and 3-nitrotyrosine. Clin Chem Lab Med. 2001; 39:491-493 28. Ertabak A, Kutluay T, Unlu A, et al. The effects of desferrioxamine on peroxynitrite-induced oxidative damage in erythrocytes. Cell Biochem Funct 2004; 22: 149–152
29. Serpersu E, Ciliv G. Some properties of (Na+K+)-dependent adenosinetriphosphatase from human erythrocytes. Biochem Med. 1978; 20: 31-39 30. Feelisch M., Noack E.A. Correlation between Nitric Oxide Formation during Degradation of Organic Nitrates and Activation of Guanylate Cyclase, Eur J Pharmacol 1987; 139:19-30
31. Knowles RG, Marrett M, Salter M, et al. Differential induction of brain, lung and liver nitric oxide synthase by endotoxin in the rat. Biochem J 1990; 270:833-836 32. Liu S, Adcock IM, Old RW, et al. Lipopolysaccharide treatment in vivo induces widespread tissue expression of inducible nitric oxide synthase mRNA. Biochem Biophys Res Commun 1993; 196:1208-1213
33. Yang F, Comtois AS, Fang L, et al. Nitric oxide derived-nitrate anion contributes to endotoxic shock and multiple organ injury/dysfunction. Crit Care Med 2002; 30: 650-657
34. Paya D, Stoclet JC. Involvement of bradykinin and nitric oxide in the early hemodynamic effects of lipopolysaccharide in rats. Shock 1995; 3:376-379
35. Morrissey JJ, McCracken R, Kaneto H, et al. Location of an inducible nitric oxide synthase mRNA in the normal kidney. Kidney Int 1994; 45:998-1005
36. Mayeux PR, Garner HR, Gibson JD, et al. Effect of lipopolysaccharide on nitric oxide synthase activity in rat proximal tubules. Biochem Pharm 1995; 49:115-118
37. D’Ambrossio SM, Gibson-D’Ambrossio RE, Brady T, et al. Mechanisms of nitric oxide-induced cytotoxicity in normal human hepatocytes. Environ Mol Mutagen 2001; 37:46-54
38. Song YS, Park EH, Hur GM, et al. Caffeic acid phenethyl ester inhibits nitric oxide synthase gene expression and enzyme activity. Cancer Lett 2002;175(1):53-61
39. Celik S, Erdogan S. Caffeic acid phenethyl ester (CAPE) protects brain against oxidative stress and inflammation induced by diabetes in rats. Mol Cell Biochem 2008; 312(1-2):39-46
40. Kassim M, Mansor M, Kamalden TA, et al. Caffeic acid phenethyl ester (CAPE): scavenger of peroxynitrite in vitro and in sepsis models. Shock 2014; 42(2):154-60
41. Çakır T, Özkan E, Dulundu E, et al. Caffeic acid phenethyl ester (CAPE) prevents methotrexate-induced hepatorenal oxidative injury in rats. J Pharm Pharmacol 2011; 63(12):1566-71

Toplam 37 adet kaynakça vardır.

Ayrıntılar

Birincil Dil	İngilizce
Konular	Klinik Tıp Bilimleri
Bölüm	Araştırma Makaleleri
Yazarlar	Leyla Çimen 0000-0002-4730-5595 Behzat Çimen Aysun Çetin
Proje Numarası	TDK-2016-6596
Yayımlanma Tarihi	29 Eylül 2020
Yayımlandığı Sayı	Yıl 2020 Cilt: 1 Sayı: 2

Kaynak Göster

Vancouver	Çimen L, Çimen B, Çetin A. Nitrotyrosine formation, iNOS and the Na+,K+-ATPase activities in sepsis: The possible effects of CAPE. Exp Appl Med Sci. 2020;1(2):52-9.

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