Öz
Amaç: Ağız kanserleri, ağızdaki yapıları veya dokuları etkileyen malign bir neoplazmdır. Oral skuamoz hücreli karsinoma deri, dudaklar ve oral kavitede mukoz membranda en sık görülen neoplazmdır. Haplophyllum buxbaumii, çok yıllık otsu bir bitkidir ve antioksidan özellikleri olduğu bildirilmiştir. Çalışmanın amacı Haplophyllum’un oral skuamoz kanser hücreleri üzerindeki antikanser etkilerini değerlendirmektir.
Gereç ve Yöntem: Haplophyllum buxbaumii bitkisinden elde edilen ekstrenin skuamöz hücreli karsinom hücreleri üzerine etkileri, hücre canlılık testleri, total protein sayımı ve Elisa yöntemiyle caspase-3, Baxve Bcl-2 aktivitesi ile incelenmiştir.
Bulgular: Çalışmanın sonuçlarına göre Haplophyllum ekstresi uygulaması, kontrollerle karşılaştırıldığında skuamöz hücreli karsinom hücrelerinin canlılığını azaltırken apoptotic yolağı da aktive etmiştir.
Sonuç: Haplophyllumbuxbaumii bitki ekstresini skuamöz hücreli karsinom hücreleri üzerinde toksik etki göstermiş olup bu kanserin terapisine yönelik çalışmalara katkı sağlayacaktır. Çalışmamız bu bitkinin antikanser potansiyeli olduğunu göstermektedir.
Anahtar Kelimeler
Kaynakça
- 1. Jane-Salas E, Chimenos-Kustner E, Lopez-Lopez J, Rosello-Llabres X (2003) Importance of diet in the prevention of oral cancer. Med Oral 8 (4):260-268
- 2. Kutler DI, Auerbach AD, Satagopan J, Giampietro PF, Batish SD, Huvos AG, Goberdhan A, Shah JP, Singh B (2003) High incidence of head and neck squamous cell carcinoma in patients with Fanconi anemia. Arch Otolaryngol Head Neck Surg 129 (1):106-112. doi:10.1001/archotol.129.1.106
- 3. Warnakulasuriya S (2009) Global epidemiology of oral and oropharyngeal cancer. Oral Oncol 45 (4-5):309-316. doi:10.1016/j.oraloncology.2008.06.002
- 4. Falaki F, Dalirsani Z, Pakfetrat A, Falaki A, Saghravanian N, Nosratzehi T, Pazouki M (2011) Clinical and histopathological analysis of oral squamous cell carcinoma of young patients in Mashhad, Iran: a retrospective study and review of literature. Med Oral Patol Oral Cir Bucal 16 (4):e473-477. doi:10.4317/medoral.16.e473
- 5. Singh S, Sharma B, Kanwar SS, Kumar A (2016) Lead Phytochemicals for Anticancer Drug Development. Front Plant Sci 7:1667. doi:10.3389/fpls.2016.01667
- 6. Prieto JM, Recio MC, Giner RM, Manez S, Massmanian A, Waterman PG, Rios JL (1996) Topical anti-inflammatory lignans from Haplophyllum hispanicum. Z Naturforsch C J Biosci 51 (9-10):618-622. doi:10.1515/znc-1996-9-1002
- 7. Kaplan HM, Singirik E, Erdogan KE, Doran F (2017) Protective effect of alpha-linolenic acid on gentamicin-induced ototoxicity in mice. Somatosens Mot Res 34 (3):145-150
- 8. Kaplan HM, Izol V, Aridogan IA, Olgan E, Yegani AA, Pazarci P, Singirik E (2016) Protective Effect of Hypericum perforatum Extract on Gentamicin Induced Nephrotoxicity in Mice. Int J Pharmacol 12 (6):663-668
- 9. Izol V, Aridogan IA, Tansug Z, Doran F, Erdogan KE, Kaplan HM, Singirik E, Ertug P, Pazarci P (2019) Hypericum perforatum Extract Attennuates Gentamicin Induced Oxidative Stress, Apoptosis and Oedema in Kidney. Int J Pharmacol 15 (1):66-73
- 10. Choromanska A, Kulbacka J, Harasym J, Dubinska-Magiera M, Saczko J (2017) ANTICANCER ACTIVITY OF OAT beta-GLUCAN IN COMBINATION WITH ELECTROPORATION ON HUMAN CANCER CELLS. Acta Pol Pharm 74 (2):616-623
- 11. Kocyigit A, Koyuncu I, Dikilitas M, Bahadori F, Turkkan B (2016) Cytotoxic, genotoxic and apoptotic effects of naringenin-oxime relative to naringenin on normal and cancer cell lines. Asian Pac J Trop Bio 6 (10):872-880
- 12. Buyel JF (2018) Plants as sources of natural and recombinant anti-cancer agents. Biotechnology Advances 36 (2):506-520
- 13. Jain KK (2007) Cancer biomarkers: current issues and future directions. Curr Opin Mol Ther 9 (6):563-571
- 14. Sun J, Wei Q, Zhou Y, Wang J, Liu Q, Xu H (2017) A systematic analysis of FDA-approved anticancer drugs. BMC Syst Biol 11 (Suppl 5):87. doi:10.1186/s12918-017-0464-7
Öz
Purpose: Oral cancers are malignant neoplasms affecting the structures or tissues in the mouth and oral squamous carcinoma is the most common neoplasm of the skin, lips and oral cavity mucous membrane. It has been suggested that Haplophyllumbuxbaumii, which is a perennial herbaceous plant, has antioxidant activity. The aim of this in vitro study is to investigate the anticancer effects of Haplophyllum on oral squamous cancer cell lines.
Materials and Methods: The effects of Haplophyllumbuxbaumii plant extract on squamous cell carcinoma were evaluated by Cell viability Assay, Total Protein Quantification and by activity of cleaved caspase-3, Bax and Bcl-2 determined by ELISA method.
Results: The results of the tests showed that Haplophyllum extract application reduced the viability of squamous cell carcinoma cells while activating the apoptotic pathway when compared to the controls.
Conclusion: The findings of the study suggest that Haplophyllumbuxbaumii plant extract may have some anticancer potential to contribute to the studies on squamous cell carcinoma therapy.
Anahtar Kelimeler
Kaynakça
- 1. Jane-Salas E, Chimenos-Kustner E, Lopez-Lopez J, Rosello-Llabres X (2003) Importance of diet in the prevention of oral cancer. Med Oral 8 (4):260-268
- 2. Kutler DI, Auerbach AD, Satagopan J, Giampietro PF, Batish SD, Huvos AG, Goberdhan A, Shah JP, Singh B (2003) High incidence of head and neck squamous cell carcinoma in patients with Fanconi anemia. Arch Otolaryngol Head Neck Surg 129 (1):106-112. doi:10.1001/archotol.129.1.106
- 3. Warnakulasuriya S (2009) Global epidemiology of oral and oropharyngeal cancer. Oral Oncol 45 (4-5):309-316. doi:10.1016/j.oraloncology.2008.06.002
- 4. Falaki F, Dalirsani Z, Pakfetrat A, Falaki A, Saghravanian N, Nosratzehi T, Pazouki M (2011) Clinical and histopathological analysis of oral squamous cell carcinoma of young patients in Mashhad, Iran: a retrospective study and review of literature. Med Oral Patol Oral Cir Bucal 16 (4):e473-477. doi:10.4317/medoral.16.e473
- 5. Singh S, Sharma B, Kanwar SS, Kumar A (2016) Lead Phytochemicals for Anticancer Drug Development. Front Plant Sci 7:1667. doi:10.3389/fpls.2016.01667
- 6. Prieto JM, Recio MC, Giner RM, Manez S, Massmanian A, Waterman PG, Rios JL (1996) Topical anti-inflammatory lignans from Haplophyllum hispanicum. Z Naturforsch C J Biosci 51 (9-10):618-622. doi:10.1515/znc-1996-9-1002
- 7. Kaplan HM, Singirik E, Erdogan KE, Doran F (2017) Protective effect of alpha-linolenic acid on gentamicin-induced ototoxicity in mice. Somatosens Mot Res 34 (3):145-150
- 8. Kaplan HM, Izol V, Aridogan IA, Olgan E, Yegani AA, Pazarci P, Singirik E (2016) Protective Effect of Hypericum perforatum Extract on Gentamicin Induced Nephrotoxicity in Mice. Int J Pharmacol 12 (6):663-668
- 9. Izol V, Aridogan IA, Tansug Z, Doran F, Erdogan KE, Kaplan HM, Singirik E, Ertug P, Pazarci P (2019) Hypericum perforatum Extract Attennuates Gentamicin Induced Oxidative Stress, Apoptosis and Oedema in Kidney. Int J Pharmacol 15 (1):66-73
- 10. Choromanska A, Kulbacka J, Harasym J, Dubinska-Magiera M, Saczko J (2017) ANTICANCER ACTIVITY OF OAT beta-GLUCAN IN COMBINATION WITH ELECTROPORATION ON HUMAN CANCER CELLS. Acta Pol Pharm 74 (2):616-623
- 11. Kocyigit A, Koyuncu I, Dikilitas M, Bahadori F, Turkkan B (2016) Cytotoxic, genotoxic and apoptotic effects of naringenin-oxime relative to naringenin on normal and cancer cell lines. Asian Pac J Trop Bio 6 (10):872-880
- 12. Buyel JF (2018) Plants as sources of natural and recombinant anti-cancer agents. Biotechnology Advances 36 (2):506-520
- 13. Jain KK (2007) Cancer biomarkers: current issues and future directions. Curr Opin Mol Ther 9 (6):563-571
- 14. Sun J, Wei Q, Zhou Y, Wang J, Liu Q, Xu H (2017) A systematic analysis of FDA-approved anticancer drugs. BMC Syst Biol 11 (Suppl 5):87. doi:10.1186/s12918-017-0464-7
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Konular | Klinik Tıp Bilimleri (Diğer) |
| Bölüm | Araştırma |
| Yazarlar | |
| Yayımlanma Tarihi | 30 Eylül 2020 |
| Kabul Tarihi | 3 Temmuz 2020 |
| Yayımlandığı Sayı | Yıl 2020 Cilt: 45 Sayı: 3 |
