Araştırma Makalesi
Kısa Süreli Saklanan Koç Spermasına İlave Edilen Sığır Serum Albumininin (BSA) Farklı Dozlarının Sperma Kalitesi ve DNA Hasarı Üzerine Etkisi
Öz
Bu çalışmanın amacı kısa süreli (5 °C)’de saklanan Merinos koç spermasında 0, 24, 48 ve 72. saatlerde sığır serum albümininin (BSA) sperma kalitesi ve DNA hasarı üzerindeki etkilerini belirlemektir. Ejekülatlar beş baş Merinos ırkı koçtan suni vajen ile toplandı ve değerlendirmeler 37 °C'de yapıldı. Tüm çalışma altı kez tekrar edildi. Toplanan koç sperması örnekleri dörde bölündü ve Tris temelli sulandırıcı ile sulandırılan spermaya (0, 2, 4 ve 6 mg/ml) dozlarında BSA eklendi ve motilite, anormal spermatozoon oranı, plazma membran bütünlüğü ve canlılık, akrozom bütünlüğü ve DNA hasarı (COMET testi) belirlendi. Çalışma gruplarından 4 mg/ml BSA içeren grup, 72 saatlik depolamaya kadar, kontrol ile karşılaştırıldığında daha yüksek motilite yüzdeleri, plazma membran bütünlüğü ve canlılık gösterdi (P<0.05). Araştırmamızın 24 ve 48. saatlerinde DNA hasarı BSA’nın tüm dozlarında kontrol grubuna göre daha düşük bulundu. Bununla beraber anormal spermatozoon oranları yönünden BSA’nın tüm dozlarında 24, 48 ve 72 saatler sonunda kontrol grubuna göre daha düşük değerler olduğu görüldü (P<0.05). Sonuç olarak, kısa süreli saklamada koç sperma parametreleri için özellikle 4 mg/ml BSA eklemenin yararlı olduğu görülmüştür.
Anahtar Kelimeler
Destekleyen Kurum
Afyon Kocatepe Üniversitesi Bilimsel Araştırmalar Proje Koordinasyon Birimi
Proje Numarası
17.KARİYER.68
Kaynakça
- 1. Salamon S., Maxwell WMC., 2000. Storage of ram semen. Anim Reprod Sci, 62, 77-111. 2. Johnson LA., Weitze KF., Fiser P., Maxwell WMC., 2000. Storage of boar semen. Anim Reprod Sci, 62, 143-172. 3. Huo LJ., Yue KZ., Yang ZM., 2002. Characterization of viability, mitochondrial activity, acrosomal integrity and capacitation status in boar sperm during in vitro storage at different ambient temperatures. Reprod Fert Develop, 14, 509-514. 4. Akhter S., Rakha BA., Iqbal R., Ansari MS., 2014. Effect of bovine serum albumin on motility, plasmalemma, viability and chromatin integrity of buffalo bull spermatozoa and chromatin integrity of buffalo bull spermatozoa. Pakistan J Zool, 46, 115-120. 5. Fu J., Li Y., Wang L., Zhen L., Yang Q., Li P., Li X., 2017. Bovine serum albuminand skim-milk improve boar sperm motility by enhancing energy metabolism and protein modifications during liquid storage at 17 oC. Theriogenology, 102, 87-97. 6. Sarıözkan S., Türk G., Cantürk F., Yay A., Eken A., Akçay A., 2013. Theeffect of bovine serum albümin and fetal calf serum on sperm quality, DNA fragmentation and lipid peroxidation of the liquid stored rabbit semen. Cryobiology, 67, 1-6. 7. Uysal O., Bucak MN., 2007. Effect of oxidized serum albumin, cysteine and lycopene on the quality of frozen thawed ram semen. Acta Vet Brno, 76, 383-390. 8. Fardin A., Abbas F., Abbas K., 2010. Effects of cholesterol-loaded cyclodextrin during freezing step of cryopreservation with TCGY extender containing bovine serum albumin on quality of goat spermatozoa. Cryobiology, 61, 94-99. 9. Hughes CM., Lewis SEM., Mckelvey-Martin VJ., Thompson W., 1997. Reproducibility of human sperm DNA measurements using the alkaline single cell gel electrophoresis assay. Mutat Res, 374, 261-268. 10. Yeni D., Avdatek F., 2017. Kısa süreli saklanan epidydimal Anadolu Mandası spermasına ilave edilen karnosik asitin etkisi. Kocatepe Vet J, 10, 187-195. 11. Tamuli MK., Watson PF., 1994. Use of a simple staining technique to distinguish acrosomal changes in the live sperm sub-population. Anim Reprod Sci, 35, 247-254. 12. Avdatek F., Yeni D., Gündoğan M., 2018. Merinos koçlarda spermaya katılan antioksidanların kısa süreli saklama sırasında spermatolojik parametreler ve DNA hasarı üzerine etkileri. Kocatepe Vet J, 11, 126-133. 13. Yeni D., İnanç ME., Avdatek F., Tuncer PB., Çil B., Türkmen R., Taşdemir U., 2018. Supplementation of rosmarinic acid has reduced oxidative stress on bull spermatozoa following the freze thawing process. CryoLetters, 39, 156-165. 14. Maxwell WMC., Salamon S., 1993. Liquid storage of ram semen. Reprod Fertil Dev, 5, 613-638. 15. Sarıözkan S., Tuncer PB., Bucak MN., Ulutaş PA., 2009. Influence of various antioxidants on microscopic oxidative stress indicators and fertilizing ability of frozen-thawed bull semen. Acta Vet Brno, 78, 463-469. 16. Alçay S., Gökce E., Toker MB., Onder NT., Ustuner B., Uzabacı E., Gul Z., Cavus S., 2016. Freeze-dried egg yolk based extenders containing various antioxidants improve post-thawing quality and incubation resilience of goat spermatozoa. Cryobiology, 72, 269-273. 17. Alçay S., Toker MB., Gökçe E., Önder NT., Üstüner B., Nur Z., 2019. Long term incubation resilience of post-thaw ram semen diluted with lecithin based extener supplemented with bovine serum albumin. Kafkas Univ Vet Fak Derg, 25, 291-297. 18. Uysal O., Bucak MN., Yavaş İ., Varışlı Ö., 2007. Effect of various antioxidants on the quality of frozen-thawed bull semen. J Anim Vet Adv, 6, 1362-1366. 19. El-Kon I., 2011. Testing usability of bovine serum albumin (BSA) for preservation of Egyptian buffalo semen. American-Eurasian J Agric Environ Sci, 11, 495-502. 20. Gökçe E., Alçay S., Gül Z., 2017. Positive effect of BSA supplemented soybean lecithin based extender on liquid storage of ram semen at 5 °C. Ankara Üniv Vet Fak Derg, 64, 313-320. 21. Çoyan K., Başpınar N., Bucak MN., Akalın PP., Ataman MB., Ömür AD., Güngör S., Küçükgünay S., Özkalp B., Sarıözkan S., 2010. Influence of methionine and dithioerythritol on sperm motility, lipid peroxidation and antioxidant capacities during liquid storage of ram semen. Res Vet Sci, 89, 426-431. 22. Gündoğan M., Yeni D., Avdatek F., Fidan AF., 2010. Influence of sperm concentration on themotility, morphology, membrane and DNA integrity along with oxidative stress parameters of ram sperm during liquid storage. Anim Reprod Sci, 122, 200-207. 23. Sarıözkan S., Türk G., Cantürk F., Yay A., Eken A., Akçay A., 2013. The effect of bovine serum albümin and fetal calf serum on sperm quality, DNA fragmentation and lipid peroxidation of the liquid stored rabbit semen. Cryobiology, 67, 1-6. 24. Davis BK., Byrne R., Hun B., 1979. Studies on the mechanism of capacitation. II. Evidence for lipid transfer between plasma membrane of rat sperm and serum albümin during capacitation in vitro. Biochim Biophys Acta, 558, 257-266. 25. Alçay S., Toker MB., Gökçe E., Üstüner B., Tekin Önder N., Sağırkaya H., Nur Z., Soylu M., 2015. Successful ram semen cryopreservation with lyophilized egg yolk-based extender. Cryobiology, 71, 329-333. 26. Bouayed J., Bohn T., 2010. Exogenous antioxidants--Double-edged swords in cellular redox state: Health beneficial effects at physiologic doses versus deleterious effects at high doses. Oxid Med Cell Longev, 3, 228-237. 27. İnanç ME., Çil B., Yeni D., Avdatek F., Orakçı D., Tuncer PB., Türkmen R., Taşdemir U., 2019. The effect of green tea extract supplementation in bull bemen cryopreservation. Kafkas Univ Vet Fak Derg, 25, 703-708. 28. Avdatek F., Yeni D., İnanç ME.,Çil B., Tuncer PB., Türkmen R., Taşdemir U., 2018. Supplementation of quercetin for advanced DNA integrity in bull semen cryopreservation. Andrologia, 50, 1-7.
The Effect of Different Concentrations of Bovine Serum Albumin on Sperm Quality and DNA Damage in Short Term Stored Ram Semen
Öz
In the present research the aim was emphasized to find out the effects of bovine serum albumin (BSA) on quality of sperm and DNA damage at 0, 24 th, 48 th and 72 th hours in Merino ram semen at short term preservation (5°C). Ejaculates were obtained from five Merino Rams which were collected by artificial vagina and all the samples were pooled at 37°C for detection. All the procedures were applied six times concurrently. Different concentrations (0, 2, 4 and 6 mg/ml) of BSA with Tris-based extender was used for dilution on semen samples. Sperm motility, abnormality, plasma membrane integrity, acrosome integrity and % DNA in tail (COMET assay) content were measured and analyzed. The extender with 4 mg/ml of BSA had both higher motility percentages and plasma membrane integrity, when compare with the control, up to 72 hours (P<0.05). At the 24th and 48th hours of the study, DNA damage was lower in all doses of BSA than in the control group. In addition, in terms of abnormal spermatozoon rates, all doses of BSA were lower than the control group at 24, 48 and 72 hours (P<0.05). In conclusion, it was found to be especially useful to add 4 mg / ml BSA for the ram semen parameters in short term storage.
Anahtar Kelimeler
Proje Numarası
17.KARİYER.68
Kaynakça
- 1. Salamon S., Maxwell WMC., 2000. Storage of ram semen. Anim Reprod Sci, 62, 77-111. 2. Johnson LA., Weitze KF., Fiser P., Maxwell WMC., 2000. Storage of boar semen. Anim Reprod Sci, 62, 143-172. 3. Huo LJ., Yue KZ., Yang ZM., 2002. Characterization of viability, mitochondrial activity, acrosomal integrity and capacitation status in boar sperm during in vitro storage at different ambient temperatures. Reprod Fert Develop, 14, 509-514. 4. Akhter S., Rakha BA., Iqbal R., Ansari MS., 2014. Effect of bovine serum albumin on motility, plasmalemma, viability and chromatin integrity of buffalo bull spermatozoa and chromatin integrity of buffalo bull spermatozoa. Pakistan J Zool, 46, 115-120. 5. Fu J., Li Y., Wang L., Zhen L., Yang Q., Li P., Li X., 2017. Bovine serum albuminand skim-milk improve boar sperm motility by enhancing energy metabolism and protein modifications during liquid storage at 17 oC. Theriogenology, 102, 87-97. 6. Sarıözkan S., Türk G., Cantürk F., Yay A., Eken A., Akçay A., 2013. Theeffect of bovine serum albümin and fetal calf serum on sperm quality, DNA fragmentation and lipid peroxidation of the liquid stored rabbit semen. Cryobiology, 67, 1-6. 7. Uysal O., Bucak MN., 2007. Effect of oxidized serum albumin, cysteine and lycopene on the quality of frozen thawed ram semen. Acta Vet Brno, 76, 383-390. 8. Fardin A., Abbas F., Abbas K., 2010. Effects of cholesterol-loaded cyclodextrin during freezing step of cryopreservation with TCGY extender containing bovine serum albumin on quality of goat spermatozoa. Cryobiology, 61, 94-99. 9. Hughes CM., Lewis SEM., Mckelvey-Martin VJ., Thompson W., 1997. Reproducibility of human sperm DNA measurements using the alkaline single cell gel electrophoresis assay. Mutat Res, 374, 261-268. 10. Yeni D., Avdatek F., 2017. Kısa süreli saklanan epidydimal Anadolu Mandası spermasına ilave edilen karnosik asitin etkisi. Kocatepe Vet J, 10, 187-195. 11. Tamuli MK., Watson PF., 1994. Use of a simple staining technique to distinguish acrosomal changes in the live sperm sub-population. Anim Reprod Sci, 35, 247-254. 12. Avdatek F., Yeni D., Gündoğan M., 2018. Merinos koçlarda spermaya katılan antioksidanların kısa süreli saklama sırasında spermatolojik parametreler ve DNA hasarı üzerine etkileri. Kocatepe Vet J, 11, 126-133. 13. Yeni D., İnanç ME., Avdatek F., Tuncer PB., Çil B., Türkmen R., Taşdemir U., 2018. Supplementation of rosmarinic acid has reduced oxidative stress on bull spermatozoa following the freze thawing process. CryoLetters, 39, 156-165. 14. Maxwell WMC., Salamon S., 1993. Liquid storage of ram semen. Reprod Fertil Dev, 5, 613-638. 15. Sarıözkan S., Tuncer PB., Bucak MN., Ulutaş PA., 2009. Influence of various antioxidants on microscopic oxidative stress indicators and fertilizing ability of frozen-thawed bull semen. Acta Vet Brno, 78, 463-469. 16. Alçay S., Gökce E., Toker MB., Onder NT., Ustuner B., Uzabacı E., Gul Z., Cavus S., 2016. Freeze-dried egg yolk based extenders containing various antioxidants improve post-thawing quality and incubation resilience of goat spermatozoa. Cryobiology, 72, 269-273. 17. Alçay S., Toker MB., Gökçe E., Önder NT., Üstüner B., Nur Z., 2019. Long term incubation resilience of post-thaw ram semen diluted with lecithin based extener supplemented with bovine serum albumin. Kafkas Univ Vet Fak Derg, 25, 291-297. 18. Uysal O., Bucak MN., Yavaş İ., Varışlı Ö., 2007. Effect of various antioxidants on the quality of frozen-thawed bull semen. J Anim Vet Adv, 6, 1362-1366. 19. El-Kon I., 2011. Testing usability of bovine serum albumin (BSA) for preservation of Egyptian buffalo semen. American-Eurasian J Agric Environ Sci, 11, 495-502. 20. Gökçe E., Alçay S., Gül Z., 2017. Positive effect of BSA supplemented soybean lecithin based extender on liquid storage of ram semen at 5 °C. Ankara Üniv Vet Fak Derg, 64, 313-320. 21. Çoyan K., Başpınar N., Bucak MN., Akalın PP., Ataman MB., Ömür AD., Güngör S., Küçükgünay S., Özkalp B., Sarıözkan S., 2010. Influence of methionine and dithioerythritol on sperm motility, lipid peroxidation and antioxidant capacities during liquid storage of ram semen. Res Vet Sci, 89, 426-431. 22. Gündoğan M., Yeni D., Avdatek F., Fidan AF., 2010. Influence of sperm concentration on themotility, morphology, membrane and DNA integrity along with oxidative stress parameters of ram sperm during liquid storage. Anim Reprod Sci, 122, 200-207. 23. Sarıözkan S., Türk G., Cantürk F., Yay A., Eken A., Akçay A., 2013. The effect of bovine serum albümin and fetal calf serum on sperm quality, DNA fragmentation and lipid peroxidation of the liquid stored rabbit semen. Cryobiology, 67, 1-6. 24. Davis BK., Byrne R., Hun B., 1979. Studies on the mechanism of capacitation. II. Evidence for lipid transfer between plasma membrane of rat sperm and serum albümin during capacitation in vitro. Biochim Biophys Acta, 558, 257-266. 25. Alçay S., Toker MB., Gökçe E., Üstüner B., Tekin Önder N., Sağırkaya H., Nur Z., Soylu M., 2015. Successful ram semen cryopreservation with lyophilized egg yolk-based extender. Cryobiology, 71, 329-333. 26. Bouayed J., Bohn T., 2010. Exogenous antioxidants--Double-edged swords in cellular redox state: Health beneficial effects at physiologic doses versus deleterious effects at high doses. Oxid Med Cell Longev, 3, 228-237. 27. İnanç ME., Çil B., Yeni D., Avdatek F., Orakçı D., Tuncer PB., Türkmen R., Taşdemir U., 2019. The effect of green tea extract supplementation in bull bemen cryopreservation. Kafkas Univ Vet Fak Derg, 25, 703-708. 28. Avdatek F., Yeni D., İnanç ME.,Çil B., Tuncer PB., Türkmen R., Taşdemir U., 2018. Supplementation of quercetin for advanced DNA integrity in bull semen cryopreservation. Andrologia, 50, 1-7.
Toplam 1 adet kaynakça vardır.
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Konular | Sağlık Kurumları Yönetimi |
| Bölüm | Araştırma Makaleleri |
| Yazarlar | |
| Proje Numarası | 17.KARİYER.68 |
| Yayımlanma Tarihi | 25 Aralık 2019 |
| Yayımlandığı Sayı | Yıl 2019 Cilt: 14 Sayı: 3 |