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Optimization of Culture Conditions for Total Carotenoid Amount Using Response Surface Methodology in Green Microalgae / Ankistrodesmus convolutus

Yıl 2022, Cilt: 37 Sayı: 1, 29 - 37, 01.01.2022

Neslihan Şener Zeliha Demirel Esra İmamoğlu Meltem Dalay

https://doi.org/10.26650/ASE2020785091

Öz

Commercial carotenoids of green microalgae have become significant especially for their applica-tions in the cosmetic, pharmaceutical, food and feed industries. Effects of physical and chemical parameters on carotenoid contents in isolated microalgal species have been investigated. The variables of shaking rate, nitrogen concentration and light intensity affect biomass production and the synthesis of carotenoids in the green microalgae were investigated using the statistical design by Box-Behnken (BBD) employing Response Surface Methodology (RSM). Furthermore, the opti-mized cultivation conditions using BBD for Chlorella vulgaris, Ankistrodesmus convolutus, Dunaliel-la salina, Tetraselmis striata were determined using the spectrophotometric method to enhance carotenoid concentration. A. convolutus within the green algae was detected with the highest ca-rotenoid concentration. The optimum conditions results indicated that the growth of A. convolutus(0.55 mg/L) and production of total carotenoids (25.1138 mg/g biomass) were found at the stirrer rate of 100 rpm under the light intensity of 100 μE/m2s, and in the nutrient component of 8.82 mM NaNO3. These conditions were validated experimentally for total carotenoid yield (24.13 mg/g biomass). After that the production was performed in a flat-plate photobioreactor with a volume of 6L based on the optimized conditions and the carotenoid profile was defined by HPLC-DAD using standards such as violaxanthin, astaxanthin and β-carotene. This study proposes that the RSM ap-proach can be used to define optimal conditions for large-scale production of carotenoids by A. convolutus.

Anahtar Kelimeler

Chlorella vulgaris Ankistrodesmus convolutus Dunaliella salina Tetraselmis striata Carotenoid Optimization Photobioreactor

Destekleyen Kurum

Scientific and Technological Research Council of Turkey-TUBITAK and Ege University Scientific Research Projects-EGE BAP

Proje Numarası

TUBITAK (116Y023) and EGE BAP (17BIL008)

Teşekkür

The authors would like to thank Assistant Professor Tuğba Keskin Gündoğdu for her helping in statistic software.

Kaynakça

  • Ambati, R. R., Gogisetty, D., Aswathanarayana, R. G., Ravi, S., Bikkina, P. N., Bo, L., & Yuepeng, S. (2019). Industrial potential of carotenoid pigments from microalgae: Current trends and future prospects. Critical reviews in food science and nutrition, 59(12), 1880-1902. [CrossRef] google scholar
  • Bajwa, K., Bishnoi, N. R., Kirrolia, A., Gupta, S., & Selvan, S. T. (2019). Response surface methodology as a statistical tool for optimization of physio-biochemical cellular components of microalgae Chlorella pyrenoidosa for biodiesel production. Applied Water Science, 9(5), 128. [CrossRef] google scholar
  • Cezare-Gomes, E. A., del Carmen Mejia-da-Silva, L., Perez-Mora, L. S., Matsudo, M. C., Ferreira-Camargo, L. S., Singh, A. K., & de Carvalho, J. C. M. (2019). Potential of Microalgae Carotenoids for Industrial Application. Applied biochemistry and biotechnology, 188(3), 602634. [CrossRef] google scholar
  • Chen, T., & Wang, Y. (2013). Optimized astaxanthin production in Chlorella zofingiensis under dark condition by response surface methodology. Food Science and Biotechnology, 22(5), 1-8. [CrossRef] google scholar
  • Coelho, D. D. F., Tundisi, L. L., Cerqueira, K. S., Rodrigues, J. R. D. S., Mazzola, P. G., Tambourgi, E. B., & Souza, R. R. D. (2019). Microalgae: Cultivation Aspects and Bioactive Compounds. Brazilian Archives of Biology and Technology, 62. [CrossRef] google scholar
  • Demirel, Z., Imamoglu, E., Deniz, İ., & Dalay, M. C. Optimization of Cryopreservation Process Using Response Surface Methodology for Chlorella saccharophila and Chlorella zofingiensis. Celal Bayar Üniversitesi Fen Bilimleri Dergisi, 14(4), 405-412. [CrossRef] google scholar
  • Demirel, Z., Yilmaz, F. F., Ozdemir, G., & Dalay, M. C. (2018). Influence of Media and Temperature on the Growth and the Biological Activities of Desmodesmus protuberans (FE Fritsch & MF Rich) E. Hegewald. Turkish Journal of Fisheries and Aquatic Sciences, 18(10), 1195-1203. [CrossRef] google scholar
  • Di Lena, G., Casini, I., Lucarini, M., & Lombardi-Boccia, G. (2019). Carotenoid profiling of five microalgae species from large-scale production. Food research international, 120, 810-818. [CrossRef] google scholar
  • Erdoğan, A., Çağır, A., Dalay, M. C., & Eroğlu, A. E. (2015). Composition of carotenoids in Scenedesmus protuberans: Application of chromatographic and spectroscopic methods. Food analytical methods, 8(8), 1970-1978. [CrossRef] google scholar
  • Faraloni, C., & Torzillo, G. (2017). Synthesis of antioxidant carotenoids in microalgae in response to physiological stress. Carotenoids. IntechOpen, 143-157. [CrossRef] google scholar
  • Gonçalves, C. F., Menegol, T., & Rech, R. (2019). Biochemical composition of green microalgae Pseudoneochloris marina grown under different temperature and light conditions. Biocatalysis and agricultural biotechnology, 18, 101032. [CrossRef] google scholar
  • Keskin Gündoğdu, T., Deniz, I., Çalışkan, G., Şahin, E. S., & Azbar, N. (2016). Experimental design methods for bioengineering applications. Critical reviews in biotechnology, 36(2), 368-388. [CrossRef] google scholar
  • Novoveskâ, L., Ross, M. E., Stanley, M. S., Pradelles, R., Wasiolek, V., & Sassi, J. F. (2019). Microalgal carotenoids: A review of production, current markets, regulations, and future direction. Marine drugs, 17(11), 640. [CrossRef] google scholar
  • Saha, S. K., Ermis, H., & Murray, P. (2020). Marine microalgae for potential lutein production. Applied Sciences, 10(18), 6457. [CrossRef] google scholar
  • Senge, M., & Senger, H. (1990). Response of the photosynthetic apparatus during adaptation of Chlorella and Ankistrodesmus to irradiance changes. Journal of plant physiology, 136(6), 675-679. [CrossRef] google scholar
  • Singh, D. P., Khattar, J. S., Rajput, A., Chaudhary, R., & Singh, R. (2019). High production of carotenoids by the green microalga Asterarcys quadricellulare PUMCC 5.1. 1 under optimized culture conditions. PloS one, 14(9), e0221930. [CrossRef] google scholar
  • Sun, X. M., Ren, L. J., Zhao, Q. Y., Ji, X. J., & Huang, H. (2018). Microalgae for the production of lipid and carotenoids: a review with focus on stress regulation and adaptation. Biotechnology for biofuels, 11(1), 272. [CrossRef] google scholar
  • Şenaras, A. E. (2019). Parameter optimization using the surface response technique in automated guided vehicles. In Sustainable Engineering Products and Manufacturing Technologies (pp. 187-197). Academic Press. [CrossRef] google scholar
  • Şahin, S., Nasir, N. T. B. M., Erken, İ., Çakmak, Z. E., & Çakmak, T. (2019). Antioxidant composite films with chitosan and carotenoid extract from Chlorella vulgaris: optimization of ultrasonic-assisted extraction of carotenoids and surface characterization of chitosan films. Materials Research Express, 6(9), 095404. [CrossRef] google scholar
  • Takaichi, S. (2011). Carotenoids in algae: distributions, biosyntheses and functions. Marine drugs, 9(6), 1101-1118. [CrossRef] google scholar
  • Wang, L. J., Fan, Y., Parsons, R., Hu, G. R., Zhang, P. Y., & Li, F. L. (2018). A rapid method for the determination of fucoxanthin in diatom. Marine drugs, 16(1), 33. [CrossRef] google scholar
  • Wang, S., Cao, M., Wang, B., Deng, R., Gao, Y., & Liu, P. (2019). Optimization of growth requirements and scale-up cultivation of freshwater algae Desmodesmus armatus using response surface methodology. Aquaculture Research. [CrossRef] google scholar
  • Wellburn, A. R. (1994). The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. Journal of plant physiology, 144(3), 307-313. [CrossRef] google scholar

Yıl 2022, Cilt: 37 Sayı: 1, 29 - 37, 01.01.2022

Neslihan Şener Zeliha Demirel Esra İmamoğlu Meltem Dalay

https://doi.org/10.26650/ASE2020785091

Öz

Proje Numarası

TUBITAK (116Y023) and EGE BAP (17BIL008)

Kaynakça

  • Ambati, R. R., Gogisetty, D., Aswathanarayana, R. G., Ravi, S., Bikkina, P. N., Bo, L., & Yuepeng, S. (2019). Industrial potential of carotenoid pigments from microalgae: Current trends and future prospects. Critical reviews in food science and nutrition, 59(12), 1880-1902. [CrossRef] google scholar
  • Bajwa, K., Bishnoi, N. R., Kirrolia, A., Gupta, S., & Selvan, S. T. (2019). Response surface methodology as a statistical tool for optimization of physio-biochemical cellular components of microalgae Chlorella pyrenoidosa for biodiesel production. Applied Water Science, 9(5), 128. [CrossRef] google scholar
  • Cezare-Gomes, E. A., del Carmen Mejia-da-Silva, L., Perez-Mora, L. S., Matsudo, M. C., Ferreira-Camargo, L. S., Singh, A. K., & de Carvalho, J. C. M. (2019). Potential of Microalgae Carotenoids for Industrial Application. Applied biochemistry and biotechnology, 188(3), 602634. [CrossRef] google scholar
  • Chen, T., & Wang, Y. (2013). Optimized astaxanthin production in Chlorella zofingiensis under dark condition by response surface methodology. Food Science and Biotechnology, 22(5), 1-8. [CrossRef] google scholar
  • Coelho, D. D. F., Tundisi, L. L., Cerqueira, K. S., Rodrigues, J. R. D. S., Mazzola, P. G., Tambourgi, E. B., & Souza, R. R. D. (2019). Microalgae: Cultivation Aspects and Bioactive Compounds. Brazilian Archives of Biology and Technology, 62. [CrossRef] google scholar
  • Demirel, Z., Imamoglu, E., Deniz, İ., & Dalay, M. C. Optimization of Cryopreservation Process Using Response Surface Methodology for Chlorella saccharophila and Chlorella zofingiensis. Celal Bayar Üniversitesi Fen Bilimleri Dergisi, 14(4), 405-412. [CrossRef] google scholar
  • Demirel, Z., Yilmaz, F. F., Ozdemir, G., & Dalay, M. C. (2018). Influence of Media and Temperature on the Growth and the Biological Activities of Desmodesmus protuberans (FE Fritsch & MF Rich) E. Hegewald. Turkish Journal of Fisheries and Aquatic Sciences, 18(10), 1195-1203. [CrossRef] google scholar
  • Di Lena, G., Casini, I., Lucarini, M., & Lombardi-Boccia, G. (2019). Carotenoid profiling of five microalgae species from large-scale production. Food research international, 120, 810-818. [CrossRef] google scholar
  • Erdoğan, A., Çağır, A., Dalay, M. C., & Eroğlu, A. E. (2015). Composition of carotenoids in Scenedesmus protuberans: Application of chromatographic and spectroscopic methods. Food analytical methods, 8(8), 1970-1978. [CrossRef] google scholar
  • Faraloni, C., & Torzillo, G. (2017). Synthesis of antioxidant carotenoids in microalgae in response to physiological stress. Carotenoids. IntechOpen, 143-157. [CrossRef] google scholar
  • Gonçalves, C. F., Menegol, T., & Rech, R. (2019). Biochemical composition of green microalgae Pseudoneochloris marina grown under different temperature and light conditions. Biocatalysis and agricultural biotechnology, 18, 101032. [CrossRef] google scholar
  • Keskin Gündoğdu, T., Deniz, I., Çalışkan, G., Şahin, E. S., & Azbar, N. (2016). Experimental design methods for bioengineering applications. Critical reviews in biotechnology, 36(2), 368-388. [CrossRef] google scholar
  • Novoveskâ, L., Ross, M. E., Stanley, M. S., Pradelles, R., Wasiolek, V., & Sassi, J. F. (2019). Microalgal carotenoids: A review of production, current markets, regulations, and future direction. Marine drugs, 17(11), 640. [CrossRef] google scholar
  • Saha, S. K., Ermis, H., & Murray, P. (2020). Marine microalgae for potential lutein production. Applied Sciences, 10(18), 6457. [CrossRef] google scholar
  • Senge, M., & Senger, H. (1990). Response of the photosynthetic apparatus during adaptation of Chlorella and Ankistrodesmus to irradiance changes. Journal of plant physiology, 136(6), 675-679. [CrossRef] google scholar
  • Singh, D. P., Khattar, J. S., Rajput, A., Chaudhary, R., & Singh, R. (2019). High production of carotenoids by the green microalga Asterarcys quadricellulare PUMCC 5.1. 1 under optimized culture conditions. PloS one, 14(9), e0221930. [CrossRef] google scholar
  • Sun, X. M., Ren, L. J., Zhao, Q. Y., Ji, X. J., & Huang, H. (2018). Microalgae for the production of lipid and carotenoids: a review with focus on stress regulation and adaptation. Biotechnology for biofuels, 11(1), 272. [CrossRef] google scholar
  • Şenaras, A. E. (2019). Parameter optimization using the surface response technique in automated guided vehicles. In Sustainable Engineering Products and Manufacturing Technologies (pp. 187-197). Academic Press. [CrossRef] google scholar
  • Şahin, S., Nasir, N. T. B. M., Erken, İ., Çakmak, Z. E., & Çakmak, T. (2019). Antioxidant composite films with chitosan and carotenoid extract from Chlorella vulgaris: optimization of ultrasonic-assisted extraction of carotenoids and surface characterization of chitosan films. Materials Research Express, 6(9), 095404. [CrossRef] google scholar
  • Takaichi, S. (2011). Carotenoids in algae: distributions, biosyntheses and functions. Marine drugs, 9(6), 1101-1118. [CrossRef] google scholar
  • Wang, L. J., Fan, Y., Parsons, R., Hu, G. R., Zhang, P. Y., & Li, F. L. (2018). A rapid method for the determination of fucoxanthin in diatom. Marine drugs, 16(1), 33. [CrossRef] google scholar
  • Wang, S., Cao, M., Wang, B., Deng, R., Gao, Y., & Liu, P. (2019). Optimization of growth requirements and scale-up cultivation of freshwater algae Desmodesmus armatus using response surface methodology. Aquaculture Research. [CrossRef] google scholar
  • Wellburn, A. R. (1994). The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. Journal of plant physiology, 144(3), 307-313. [CrossRef] google scholar
Toplam 23 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Hidrobiyoloji
Bölüm Research Articles
Yazarlar

Neslihan Şener Bu kişi benim 0000-0002-4898-9562

Zeliha Demirel 0000-0003-3675-7315

Esra İmamoğlu 0000-0001-8759-7388

Meltem Dalay 0000-0002-1718-7292

Proje Numarası TUBITAK (116Y023) and EGE BAP (17BIL008)
Yayımlanma Tarihi 1 Ocak 2022
Gönderilme Tarihi 25 Ağustos 2020
Yayımlandığı Sayı Yıl 2022 Cilt: 37 Sayı: 1

Kaynak Göster

APA Şener, N., Demirel, Z., İmamoğlu, E., Dalay, M. (2022). Optimization of Culture Conditions for Total Carotenoid Amount Using Response Surface Methodology in Green Microalgae / Ankistrodesmus convolutus. Aquatic Sciences and Engineering, 37(1), 29-37. https://doi.org/10.26650/ASE2020785091
AMA Şener N, Demirel Z, İmamoğlu E, Dalay M. Optimization of Culture Conditions for Total Carotenoid Amount Using Response Surface Methodology in Green Microalgae / Ankistrodesmus convolutus. Aqua Sci Eng. Ocak 2022;37(1):29-37. doi:10.26650/ASE2020785091
Chicago Şener, Neslihan, Zeliha Demirel, Esra İmamoğlu, ve Meltem Dalay. “Optimization of Culture Conditions for Total Carotenoid Amount Using Response Surface Methodology in Green Microalgae / Ankistrodesmus Convolutus”. Aquatic Sciences and Engineering 37, sy. 1 (Ocak 2022): 29-37. https://doi.org/10.26650/ASE2020785091.
EndNote Şener N, Demirel Z, İmamoğlu E, Dalay M (01 Ocak 2022) Optimization of Culture Conditions for Total Carotenoid Amount Using Response Surface Methodology in Green Microalgae / Ankistrodesmus convolutus. Aquatic Sciences and Engineering 37 1 29–37.
IEEE N. Şener, Z. Demirel, E. İmamoğlu, ve M. Dalay, “Optimization of Culture Conditions for Total Carotenoid Amount Using Response Surface Methodology in Green Microalgae / Ankistrodesmus convolutus”, Aqua Sci Eng, c. 37, sy. 1, ss. 29–37, 2022, doi: 10.26650/ASE2020785091.
ISNAD Şener, Neslihan vd. “Optimization of Culture Conditions for Total Carotenoid Amount Using Response Surface Methodology in Green Microalgae / Ankistrodesmus Convolutus”. Aquatic Sciences and Engineering 37/1 (Ocak 2022), 29-37. https://doi.org/10.26650/ASE2020785091.
JAMA Şener N, Demirel Z, İmamoğlu E, Dalay M. Optimization of Culture Conditions for Total Carotenoid Amount Using Response Surface Methodology in Green Microalgae / Ankistrodesmus convolutus. Aqua Sci Eng. 2022;37:29–37.
MLA Şener, Neslihan vd. “Optimization of Culture Conditions for Total Carotenoid Amount Using Response Surface Methodology in Green Microalgae / Ankistrodesmus Convolutus”. Aquatic Sciences and Engineering, c. 37, sy. 1, 2022, ss. 29-37, doi:10.26650/ASE2020785091.
Vancouver Şener N, Demirel Z, İmamoğlu E, Dalay M. Optimization of Culture Conditions for Total Carotenoid Amount Using Response Surface Methodology in Green Microalgae / Ankistrodesmus convolutus. Aqua Sci Eng. 2022;37(1):29-37.
 Kapak Resmi İndir

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