Araştırma Makalesi
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Eisenia hortensis Sölomositlerinde Bentazone ve Chloridazon Herbisitlerinin Genotoksisitesinin Değerlendirilmesi

Yıl 2020, Cilt: 20 Sayı: 6, 963 - 967, 31.12.2020
https://doi.org/10.35414/akufemubid.827228

Öz

Bu çalışmada, Bentazone ve Chloridazon herbisitlerinin Eisenia hortensis türleri üzerindeki genotoksik etkileri araştırılmıştır. E. hortensis türleri Afyonkarahisar'daki doğal yaşam alanlarından toplanmıştır. Solucan sölomositlerinde genotoksisiteyi değerlendirmek için Comet ve Mikronükleus testi yapıldı. Bentazone herbisitlerin LD50 değeri Chloridazon için sırasıyla 236 ppm ve 76.6 ppm olarak kaydedildi. Daha sonra LD50/2, LD50 and 2XLD50 konsantrasyonlarında Bentazon ve Chloridazon herbisitleri E. hortensis'e 48 saat süreyle uygulandı. Her iki herbisitte de DNA'da konsantrasyona bağlı artış ve kromozomal hasar gözlendi (P <0.05). En yüksek DNA hasarı ve kromozomal sapmaları, diğer konsantrasyonlara ve kontrol grubuna kıyasla en yüksek dozlarda tespit edildi. Bentazone ve Chloridazon'un E. hortensis’ te DNA hasarı ve kromozomal sapmalara neden olduğu sonucuna varılmıştır.

Destekleyen Kurum

Afyon Kocatepe Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Proje Numarası

15.FEN.BİL.44

Kaynakça

  • Ahmadi F., Jamali N., Jahangard-Yekta S., Jafari S., Nouri S., Najafi F. and Rahimi-Nasrabadi M., 2011. The experimentaland theoretical QM/MM study of interaction of chloridazon herbicide with ds-DNA. Spectrochimica Acta Part A: Molecularand Biomolecular Spectroscopy, 1011, 1004-1012.
  • Aydın H., 2006. Toprak solucanlarının Çevre Toksikolojisi Yönünden Değerlendirilmesi. İstanbul Üniversites Veterinerlik Fakültesi Dergisi, 32, 75-79
  • Brkic D., Szakonyne-Pasics I., Gasic S., Teodorovic I., Raskovic B., Brkic N. and Neskovic N., 2015. Subacute and subchronic toxicity of Avalonregd mixture (bentazone+dicamba) to rats. Environmental Toxicology and Pharmacology, 13,1-30.
  • Castellanos L.R. and Hernandez T.C.S., 2007. Earthworm biomarkers of pesticide contamination: current status and perspectives. Journal of Pesticide Science 32(4),360-371
  • Charles B. K., Wıllıam W. B.., Seth R.K., Bonnıe U., and Thomas F. S., 1995. Stages of Embryonic Development of the Zebrafish. Developmental Dynamıcs,203, 253-310
  • Ciğerci, İ., Ali, M.M., Yüksek, Ş., Liman, R., 2016. Genotoxicity assessment of cobalt chloride in Eisenia hortensis earthworms coelomocytes by comet assay and micronucleus test. Chemosphere, 755, 754-757.
  • Collins A., Hartmann A., Agurell E., Beevers C., Brendler-Schwaab S., Burlinson B. and Clay P., 2003. Recommendations for conducting the in vivo alkaline Comet assay. Mutagenesis, 18(1), 45-51.
  • Gambi N., Pasteris A., Fabbri E., 2007. Acetylcholinesterase activity in the earthworm (Eisenia andrei) at different conditions of carbaryl exposure. Comparative Biochemistry & Physiology, 145 (4), 678–685.
  • Kaya B., Marcos R., Yanikoğlu A. and Creus A., 2004. Evaluation of the genotoxicity of four herbicides in the wing spot test of Drosophila melanogaster using two different strains. Mutation Research, 55, 53-62.
  • Lanno R., Wells J., Conder J., Bradham K., Basta N., 2004. The bioavailability of chemicalsin soil for earthworms. Ecotoxicol. Environ. Saf. 57 (1), 39–47.
  • Lina S., Yarong G.., Jiao N., Shanning Lou., Fujiang H. 2020. Herbicide Applications Increase Greenhouse Gas Emissions of Alfalfa Pasture in the Inland Arid Region of Northwest China. PeerJ. May 25,8, 9231.
  • Lukkari T., Taavitsainen M., Soimasuo M., Oikari A., Haimi J., 2004. Biomarker responses of the earthworm (Aporrectodea tuberculata) to copper and zinc exposure: differences between populations with and without earlier metal exposure. Environ. Pollut, 129, 377–386.
  • Reinecke S.A., Reinecke, A.J., 2004. The comet assay as biomarker of heavy metal genotoxicity in earthworms. Arch. Environ. Contam. Toxicol. 46, 208-215.
  • Reinecke S.A. and Reinecke A.J., 2007. The impact of organophosphate pesticides in orchards on earthworms in the Western Cape, South Africa. Ecotoxicol. Environ. Saf. 66 (2), 244–251.
  • Saglıo P., Olsen K.H., Bretaud S., 2001. Behavioral and olfactory responses to prochloraz, bentazone, and nicosulfuron contaminated flows in golfish. Arch Environ Contam Toxicol, 41(2), 192-200.
  • Venkateswara Rao J., Kavitha P., Jakka N.M., Sridhar V., Usman P.K., 2007. Toxicity of organophosphates on morphology andocomotor behavior in brine shrimp (Artemia salina). Arch. Environ. Contam. Toxicol. 53, 227–232.
  • Xiao H., Zhou Q.X., Liang J.D., 2004. Single and joint effects of acetochlor and urea on earthworm Eisenia foelide populations in phaiozem. Environmental Geochemistry and Health, 26, 277–283.
  • Xiao N.W., Jing B.B., Ge F., Liu, X.H., 2006. The fate of herbicide acetochlor and its toxicity to Eisenia fetida under laboratory conditions. Chemosphere, 62, 1366– 1373.
  • Zhan, Y., 2012. Effects of silver nanoparticles on bacteria and earthworms. Master's thesis , Lincoln University, ABD, 129.

Genotoxic Evaluation of Bentazone and Chloridazon Herbicides in Eisenia hortensis Coelomocytes

Yıl 2020, Cilt: 20 Sayı: 6, 963 - 967, 31.12.2020
https://doi.org/10.35414/akufemubid.827228

Öz

In current study, genotoxic effects of Bentazone and Chloridazon herbicides on Eisenia hortensis species were investigated. The species of E. hortensis were collected from the natural habitats in Afyonkarahisar. Comet assay and Micronucleus test was conducted to evaluate the genotoxicity in earthworm coelomocytes. The LD50 value of Bentazone herbicides was noted as 236 ppm and 76.6 ppm for Chloridazon, respectively. Then LD50/2, LD50 and 2XLD50 concentrations of Bentazone and Chloridazon herbicides were applied to E. hortensis for 48 h. Concentration dependent increase in DNA and chromosomal damage was observed (P < 0.05) by both herbicides. Highest DNA damage and micronucleus formation were noticed at highest doses compared to other concentrations and control group. It was concluded that Bentazone and Chloridazon induced DNA damage and chromosomal aberrations failure in E. hortensis earthworms.

Proje Numarası

15.FEN.BİL.44

Kaynakça

  • Ahmadi F., Jamali N., Jahangard-Yekta S., Jafari S., Nouri S., Najafi F. and Rahimi-Nasrabadi M., 2011. The experimentaland theoretical QM/MM study of interaction of chloridazon herbicide with ds-DNA. Spectrochimica Acta Part A: Molecularand Biomolecular Spectroscopy, 1011, 1004-1012.
  • Aydın H., 2006. Toprak solucanlarının Çevre Toksikolojisi Yönünden Değerlendirilmesi. İstanbul Üniversites Veterinerlik Fakültesi Dergisi, 32, 75-79
  • Brkic D., Szakonyne-Pasics I., Gasic S., Teodorovic I., Raskovic B., Brkic N. and Neskovic N., 2015. Subacute and subchronic toxicity of Avalonregd mixture (bentazone+dicamba) to rats. Environmental Toxicology and Pharmacology, 13,1-30.
  • Castellanos L.R. and Hernandez T.C.S., 2007. Earthworm biomarkers of pesticide contamination: current status and perspectives. Journal of Pesticide Science 32(4),360-371
  • Charles B. K., Wıllıam W. B.., Seth R.K., Bonnıe U., and Thomas F. S., 1995. Stages of Embryonic Development of the Zebrafish. Developmental Dynamıcs,203, 253-310
  • Ciğerci, İ., Ali, M.M., Yüksek, Ş., Liman, R., 2016. Genotoxicity assessment of cobalt chloride in Eisenia hortensis earthworms coelomocytes by comet assay and micronucleus test. Chemosphere, 755, 754-757.
  • Collins A., Hartmann A., Agurell E., Beevers C., Brendler-Schwaab S., Burlinson B. and Clay P., 2003. Recommendations for conducting the in vivo alkaline Comet assay. Mutagenesis, 18(1), 45-51.
  • Gambi N., Pasteris A., Fabbri E., 2007. Acetylcholinesterase activity in the earthworm (Eisenia andrei) at different conditions of carbaryl exposure. Comparative Biochemistry & Physiology, 145 (4), 678–685.
  • Kaya B., Marcos R., Yanikoğlu A. and Creus A., 2004. Evaluation of the genotoxicity of four herbicides in the wing spot test of Drosophila melanogaster using two different strains. Mutation Research, 55, 53-62.
  • Lanno R., Wells J., Conder J., Bradham K., Basta N., 2004. The bioavailability of chemicalsin soil for earthworms. Ecotoxicol. Environ. Saf. 57 (1), 39–47.
  • Lina S., Yarong G.., Jiao N., Shanning Lou., Fujiang H. 2020. Herbicide Applications Increase Greenhouse Gas Emissions of Alfalfa Pasture in the Inland Arid Region of Northwest China. PeerJ. May 25,8, 9231.
  • Lukkari T., Taavitsainen M., Soimasuo M., Oikari A., Haimi J., 2004. Biomarker responses of the earthworm (Aporrectodea tuberculata) to copper and zinc exposure: differences between populations with and without earlier metal exposure. Environ. Pollut, 129, 377–386.
  • Reinecke S.A., Reinecke, A.J., 2004. The comet assay as biomarker of heavy metal genotoxicity in earthworms. Arch. Environ. Contam. Toxicol. 46, 208-215.
  • Reinecke S.A. and Reinecke A.J., 2007. The impact of organophosphate pesticides in orchards on earthworms in the Western Cape, South Africa. Ecotoxicol. Environ. Saf. 66 (2), 244–251.
  • Saglıo P., Olsen K.H., Bretaud S., 2001. Behavioral and olfactory responses to prochloraz, bentazone, and nicosulfuron contaminated flows in golfish. Arch Environ Contam Toxicol, 41(2), 192-200.
  • Venkateswara Rao J., Kavitha P., Jakka N.M., Sridhar V., Usman P.K., 2007. Toxicity of organophosphates on morphology andocomotor behavior in brine shrimp (Artemia salina). Arch. Environ. Contam. Toxicol. 53, 227–232.
  • Xiao H., Zhou Q.X., Liang J.D., 2004. Single and joint effects of acetochlor and urea on earthworm Eisenia foelide populations in phaiozem. Environmental Geochemistry and Health, 26, 277–283.
  • Xiao N.W., Jing B.B., Ge F., Liu, X.H., 2006. The fate of herbicide acetochlor and its toxicity to Eisenia fetida under laboratory conditions. Chemosphere, 62, 1366– 1373.
  • Zhan, Y., 2012. Effects of silver nanoparticles on bacteria and earthworms. Master's thesis , Lincoln University, ABD, 129.
Toplam 19 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Mühendislik
Bölüm Makaleler
Yazarlar

Sevgi Ulukütük Bu kişi benim 0000-0002-5760-8493

İbrahim Hakkı Ciğerci 0000-0002-3626-7730

Proje Numarası 15.FEN.BİL.44
Yayımlanma Tarihi 31 Aralık 2020
Gönderilme Tarihi 17 Kasım 2020
Yayımlandığı Sayı Yıl 2020 Cilt: 20 Sayı: 6

Kaynak Göster

APA Ulukütük, S., & Ciğerci, İ. H. (2020). Genotoxic Evaluation of Bentazone and Chloridazon Herbicides in Eisenia hortensis Coelomocytes. Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi, 20(6), 963-967. https://doi.org/10.35414/akufemubid.827228
AMA Ulukütük S, Ciğerci İH. Genotoxic Evaluation of Bentazone and Chloridazon Herbicides in Eisenia hortensis Coelomocytes. Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi. Aralık 2020;20(6):963-967. doi:10.35414/akufemubid.827228
Chicago Ulukütük, Sevgi, ve İbrahim Hakkı Ciğerci. “Genotoxic Evaluation of Bentazone and Chloridazon Herbicides in Eisenia Hortensis Coelomocytes”. Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi 20, sy. 6 (Aralık 2020): 963-67. https://doi.org/10.35414/akufemubid.827228.
EndNote Ulukütük S, Ciğerci İH (01 Aralık 2020) Genotoxic Evaluation of Bentazone and Chloridazon Herbicides in Eisenia hortensis Coelomocytes. Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi 20 6 963–967.
IEEE S. Ulukütük ve İ. H. Ciğerci, “Genotoxic Evaluation of Bentazone and Chloridazon Herbicides in Eisenia hortensis Coelomocytes”, Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi, c. 20, sy. 6, ss. 963–967, 2020, doi: 10.35414/akufemubid.827228.
ISNAD Ulukütük, Sevgi - Ciğerci, İbrahim Hakkı. “Genotoxic Evaluation of Bentazone and Chloridazon Herbicides in Eisenia Hortensis Coelomocytes”. Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi 20/6 (Aralık 2020), 963-967. https://doi.org/10.35414/akufemubid.827228.
JAMA Ulukütük S, Ciğerci İH. Genotoxic Evaluation of Bentazone and Chloridazon Herbicides in Eisenia hortensis Coelomocytes. Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi. 2020;20:963–967.
MLA Ulukütük, Sevgi ve İbrahim Hakkı Ciğerci. “Genotoxic Evaluation of Bentazone and Chloridazon Herbicides in Eisenia Hortensis Coelomocytes”. Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi, c. 20, sy. 6, 2020, ss. 963-7, doi:10.35414/akufemubid.827228.
Vancouver Ulukütük S, Ciğerci İH. Genotoxic Evaluation of Bentazone and Chloridazon Herbicides in Eisenia hortensis Coelomocytes. Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi. 2020;20(6):963-7.