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In Vitro Antidiabetic, Antiinflammatory, Cytotoxic, Antioxidant and Antimicrobial Activities of Pomegranate (Punica granatum L.) Peel

Yıl 2019, Cilt: 17 Sayı: 1, 61 - 71, 26.03.2019
https://doi.org/10.24323/akademik-gida.544647

Öz

In this study, antioxidant, antimicrobial,
antidiabetic, antiinflammatory and cytotoxic properties of the pomegranate (Punica granatum L.) peel were
investigated. The composition of the phenolic compounds of the pomegranate peel
extract (33%, 78°C, 113 min) was determined and it was found to contain high amounts
of punigalagin, caffeic acid and epicatechin. At the same time, the extract was
found to have high antioxidant capacity.  It showed antimicrobial activity against
selected microorganisms
(Staphylococcus aureus, Enterococcus
faecalis
,
Escherichia
coli
, Aspergillus flavus and Aspergillus niger) and its
highest microbial resistance was found to be against S. aureus (21 mm; 1.87
mg extract
/mL
). It inhibited α-amylase and α-glucosidase enzymes and showed the antidiabetic
property. At the same time, the extract was able to inhibit xanthine oxidase
and lipoxygenase enzymes that were responsible for the inflammation and its cytotoxic
activity on breast and bone cancer cells were also observed. It was concluded
that pomegranate peel extracts could be used in food and non-food applications due
to its biological activities.

Kaynakça

  • [1] Bayram, E., Kırcı, S., Tansı, S., Yılmaz, G., Arabacı, O., Kızıl, S., Telci, İ. (2010). Tıbbi ve aromatik bitkiler üretiminin arttırılması olanakları, Türkiye Ziraat Mühendisliği VII. Teknik Kongresi, Ankara.
  • [2] Jaiswal, V., DerMarderosian, A., Porter, J.R. (2010). Anthocyanins and polyphenol oxidase from dried arils of pomegranate (Punica granatum L.). Food Chemistry, 118(1), 11-16.
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  • [5] Yusof, H., Radzı, N.A.S.M., Rıchard, R.L. (2018). Qualitative phytochemical analysis and antimicrobial activity of piper sarmentosum leaves extract against selected pathogens. Malaysian Journal of Health Sciences, 17.1.
  • [6] Wanasundara, U.N., Shahidi, F. (1998). Antioxidant and prooxidant activity of green tea extracts in marine oils. Food Chemistry, 63(3), 335-342.
  • [7] Naczk, M., Shahidi, F. (2006). Phenolics in cereals, fruits and vegetables: Occurrence, extraction and analysis. Journal of Pharmaceutical and Biomedical Analysis, 41(5), 1523-1542.
  • [8] Friedman, M. (2007). Overview of antibacterial, antitoxin, antiviral, and antifungal activities of tea flavonoids and teas. Molecular Nutrition and Food Research, 51(1): 116-134.
  • [9] Sharmila, G., Bhat, F.A., Arunkumar, R., Elumalai, P., Singh, P.R., Senthilkumar, K., Arunakaran, J. (2014). Chemopreventive effect of quercetin, a natural dietary flavonoid on prostate cancer in in vivo model. Clinical Nutrition 33(4), 718-726.
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  • [11] Mai, T.T., Thu N. N., Tien P.G., Van Chuyen, N. (2007). Alpha-glucosidase inhibitory and antioxidant activities of Vietnamase edible plants and their relationships with polyphenol contents. Journal of Nutritional Science and Vitaminology, 53(3), 267-276.
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Nar (Punica granatum L.) Kabuğunun İn Vitro Antidiyabetik, Antienflamatuar, Sitotoksik, Antioksidan ve Antimikrobiyal Aktivitesi

Yıl 2019, Cilt: 17 Sayı: 1, 61 - 71, 26.03.2019
https://doi.org/10.24323/akademik-gida.544647

Öz

Bu çalışmada nar (Punica granatum L.) kabuğunun sağlık
açısından önemli antioksidan, antimikrobiyal,
antidiyabetik, antienflamatuar ve
sitotoksik özellikleri araştırılmıştır. Nar kabukları (%33 etanol
konsantrasyonu, 78°C, 113 dakika) ekstrakte edilerek
fenolik
bileşiklerinin kompozisyonu belirlenmiş, yüksek oranda punigalajin,
kafeik asit ve epikateşin içerdiği ve
yüksek antioksidan kapasiteye sahip olduğu tespit edilmiştir. Elde edilen
ekstrakt aynı zamanda, seçilen mikroorganizmalara (
Staphylococcus aureus, Enterococcus
faecalis
,
Escherichia
coli
, Aspergillus flavus ve Aspergillus niger) karşı antimikrobiyal
etki göstermiş ve en fazla mikrobiyal direnci S.
aureus’a
(21 mm; 1.87
mg ekstrakt/mL) karşı olduğu bulunmuştur. Ekstrakt  α-amilaz ve α-glukozidaz
enzimlerini inhibe etmiş ve antidiyabetik özellik göstermiştir. Aynı zamanda
ekstraktın enflamasyondan sorumlu ksantin oksidaz ve lipoksigenaz enzimlerini
de inhibe edebildiği ve
meme ve kemik kanseri
hücreleri üzerinde sitotoksik aktiviteye sahip olduğu da gözlenmiştir. Nar
kabuğu ekstraktının belirlenen biyolojik aktiviteleri ile gıda ve gıda dışı
uygulamalarda kullanılabileceği sonucuna varılmıştır.

Kaynakça

  • [1] Bayram, E., Kırcı, S., Tansı, S., Yılmaz, G., Arabacı, O., Kızıl, S., Telci, İ. (2010). Tıbbi ve aromatik bitkiler üretiminin arttırılması olanakları, Türkiye Ziraat Mühendisliği VII. Teknik Kongresi, Ankara.
  • [2] Jaiswal, V., DerMarderosian, A., Porter, J.R. (2010). Anthocyanins and polyphenol oxidase from dried arils of pomegranate (Punica granatum L.). Food Chemistry, 118(1), 11-16.
  • [3] T.C. Başbakanlık Türkiye İstatistik Kurumu https://biruni.tuik.gov.tr/bitkiselapp/bitkisel.zul
  • [4] Samaranayaka, A.G.P., Li-Chan, E.C.Y. (2011). Food-derived peptidic antioxidants: A review of their production, assessment, and potential applications. Journal of Functional Food, 3(4), 229-254.
  • [5] Yusof, H., Radzı, N.A.S.M., Rıchard, R.L. (2018). Qualitative phytochemical analysis and antimicrobial activity of piper sarmentosum leaves extract against selected pathogens. Malaysian Journal of Health Sciences, 17.1.
  • [6] Wanasundara, U.N., Shahidi, F. (1998). Antioxidant and prooxidant activity of green tea extracts in marine oils. Food Chemistry, 63(3), 335-342.
  • [7] Naczk, M., Shahidi, F. (2006). Phenolics in cereals, fruits and vegetables: Occurrence, extraction and analysis. Journal of Pharmaceutical and Biomedical Analysis, 41(5), 1523-1542.
  • [8] Friedman, M. (2007). Overview of antibacterial, antitoxin, antiviral, and antifungal activities of tea flavonoids and teas. Molecular Nutrition and Food Research, 51(1): 116-134.
  • [9] Sharmila, G., Bhat, F.A., Arunkumar, R., Elumalai, P., Singh, P.R., Senthilkumar, K., Arunakaran, J. (2014). Chemopreventive effect of quercetin, a natural dietary flavonoid on prostate cancer in in vivo model. Clinical Nutrition 33(4), 718-726.
  • [10] Skowyra, M., Falguera, V., Gallego, G., Peiro, S., Almajano, M.P. (2014). Antioxidant properties of aqueous and ethanolic extracts of tara (Caesalpinia spinosa) pods in vitro and in model food emulsions. Journal of the Science of Food and Agriculture, 94(5), 911-918.
  • [11] Mai, T.T., Thu N. N., Tien P.G., Van Chuyen, N. (2007). Alpha-glucosidase inhibitory and antioxidant activities of Vietnamase edible plants and their relationships with polyphenol contents. Journal of Nutritional Science and Vitaminology, 53(3), 267-276.
  • [12] Tomy M. J., Sharanya C. S., Dileep K. V., Prasanth S., Sabu A., Sadasivan C., Haridas M. (2014). Derivatives form better lipoxygenase inhibitors than piperine: in vitro and in silico study. Chemical Biology and Drug Design, 85(6), 715-721.
  • [13] Kulkarni, S.G., Vijayanand, P. (2010). Effect of extraction conditions on the quality characteristics of pectin from passion fruit peel (Passiflora edulis f. flavicarpa L.). Food Science and Technology, 43(7), 1026-1031.
  • [14] Wang, R., Ding, Y., Liu, R., Xiang, L., Du, L. (2010). Pomegranate: Constituents, bioactivities and pharmacokinetics. Fruit, Vegetable and Cereal Science and Biotechnology, 4(2), 77-87.
  • [15] Prakash, C.V.S., Prakash, I. (2011). Bioactive chemical constituents from pomegranate (Punica granatum) juice, seed and peel-a review. International Journal of Research in Chemistry and Environment, 1(1), 1-18.
  • [16] Amyrgialaki, E., Makris, D.P., Mauromoustakos, A., Kefalas, P. (2014). Optimisation of the extraction of pomegranate (Punica granatum) husk phenolics using water/ethanol solvent systems and response surface methodology. Industrial Crops and Products, 59, 216-222.
  • [17] Nahar, P.P., Driscoll, M.V., Li, L., Slitt, A.L., Seeram, N.P. (2014). Phenolic mediated anti-inflammatory properties of a maple syrup extract in RAW 264.7 murine macrophages. Journal of Functional Foods, 6, 126-136.
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  • [52] Ibrahium, M.I. (2010). Efficiency of pomegranate peel extract as antimicrobial, antioxidant and protective agents. World Journal of Agricultural Sciences, 6 (4), 338-344.
  • [53] Gil, M.I., Tomas-Barberan, F.A., Hess-Pierce, B., Holcroft, D.M., Kader, A.A, (2000). Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing. Journal of Agricultural and Food Chemistry, 48(10), 4581-4589.
  • [54] MacDonald-Wicks, L.K., Wood, L.G., Garg, M.L. (2006). Methodology for the determination of biological antioxidant capacity in vitro: A review. Journal of the Science of Food and Agriculture, 86(13), 2046-2056.
  • [55] Mushtaq, M., Sultana, B., Anwar, F., Adnan, A., Rizvi, S.S. (2015). Enzyme-assisted supercritical fluid extraction of phenolic antioxidants from pomegranate peel. The Journal of Supercritical Fluids, 104, 122-131.
  • [56] Fawole, O.A., Makunga, N.P., Opara, U.L. (2012). Antibacterial, antioxidant and tyrosinase-inhibition activities of pomegranate fruit peel methanolic extract. BMC Complementary and Alternative Medicine, 12(1), 200.
  • [57] Farag, M.A., Al-Mahdy, D.A., Salah El Dine, R., Fahmy, S., Yassin, A., Porzel, A., Brandt, W. (2015). Structure activity relationships of antimicrobial gallic acid derivatives from pomegranate and acacia fruit extracts against potato bacterial wilt pathogen. Chemistry & Biodiversity, 12(6), 955-962.
  • [58] Gullon, B., Pintado, M.E., Pérez-Álvarez, J.A., Viuda-Martos, M. (2016). Assessment of polyphenolic profile and antibacterial activity of pomegranate peel (Punica granatum) flour obtained from co-product of juice extraction. Food Control, 59, 94-98.
  • [59] Dik, B. (2013). Metabolik sendromun tedavisi. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, 8(3), 259-269.
  • [60] Jung, M., Park, M., Lee, H.C., Kang, Y.H., Kang, E.S., Kim, S.K. (2006). Antidiabetic agents from medicinal plants. Current Medicinal Chemistry, 13 (10), 1203-1218.
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  • [65] Hasenah, P., Houghton, J., Soumyanath, A. (2006). α-Amylase inhibitory activity of some Malaysian plants used to treat diabetes; with particular reference to Phyllanthus amarus. Journal of Ethnopharmacology 10(3), 449-455.
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  • [69] Çam, M., İçyer, N.C., Erdoğan, F. (2014). Pomegranate peel phenolics: Microencapsulation, storage stability and potential ingredient for functional food development. LWT-Food Science and Technology, 55(1), 117-123.
  • [70] Akhtar, S., Ismail, T., Sestili, P., Riaz, M., Ismail, A., Labbe, R.G. (2016). Antioxidant, antimicrobial and urease inhibitory activities of phenolics-rich pomegranate peel hydro-alcoholic extracts. Journal of Food Biochemistry, 40(4), 550-558.
  • [71] Tew, K.D., Gate, L. (2001). Glutathione S-transferases as emerging therapeutic targets. Expert Opinion on Therapeutic Targets, 5(4), 477.
  • [72] Çağlar, H.O., Süslüer, S.Y., Kavaklı, Ş., Gündüz, C., Ertürk, B., Özkınay, F., Haydaroğlu, A. (2017). Meme kanseri kök hücrelerinde elajik asit ile indüklenmiş mRNA’ların ifadesi ve elajik asidin apoptoz üzerine etkisi. Ege Tıp Dergisi, 56(4), 183-192.
  • [73] Hwang, H.J., Park, H.J., Chung, H.J., Min, H.Y., Park, E.J., Hong, J.Y., Lee, S.K. (2005). Inhibitory effects of caffeic acid phenethyl ester on cancer cell metastasis mediated by the down-regulation of matrix metalloproteinase expression in human HT1080 fibrosarcoma cells. Journal of Nutritional Biochemistry, 17(5), 356-362.
  • [74] Yang, J.H.H., Hsia, T.C., Kuo, H.M., Chao, P.D., Chou, C.C., Wei, Y.H., Hchung, J.G. (2006). Inhibition of lung cancer cell growth by quercetin glucuronides via G2/M arrest and induction of apoptosis. Drug Metabolism and Disposition: The Biological Fate of Chemicals, 34(1), 296-304.
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  • [77] Moradzadeh, M., Roustazadeh, A., Tabarraei, A., Erfanian, S., Sahebkar, A. (2017). Epigallocatechin‐3‐gallate enhances differentiation of acute promyelocytic leukemia cells via inhibition of PML‐RARα and HDAC1. Phytotherapy Research, 3, 1-9.
Toplam 77 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Gıda Mühendisliği
Bölüm Araştırma Makaleleri
Yazarlar

Tuğba Demir 0000-0002-5195-9372

Özlem Akpınar 0000-0001-6593-8495

Haki Kara 0000-0002-0295-0582

Hüseyin Güngör Bu kişi benim 0000-0003-0309-4383

Yayımlanma Tarihi 26 Mart 2019
Gönderilme Tarihi 5 Mart 2019
Yayımlandığı Sayı Yıl 2019 Cilt: 17 Sayı: 1

Kaynak Göster

APA Demir, T., Akpınar, Ö., Kara, H., Güngör, H. (2019). Nar (Punica granatum L.) Kabuğunun İn Vitro Antidiyabetik, Antienflamatuar, Sitotoksik, Antioksidan ve Antimikrobiyal Aktivitesi. Akademik Gıda, 17(1), 61-71. https://doi.org/10.24323/akademik-gida.544647
AMA Demir T, Akpınar Ö, Kara H, Güngör H. Nar (Punica granatum L.) Kabuğunun İn Vitro Antidiyabetik, Antienflamatuar, Sitotoksik, Antioksidan ve Antimikrobiyal Aktivitesi. Akademik Gıda. Mart 2019;17(1):61-71. doi:10.24323/akademik-gida.544647
Chicago Demir, Tuğba, Özlem Akpınar, Haki Kara, ve Hüseyin Güngör. “Nar (Punica Granatum L.) Kabuğunun İn Vitro Antidiyabetik, Antienflamatuar, Sitotoksik, Antioksidan Ve Antimikrobiyal Aktivitesi”. Akademik Gıda 17, sy. 1 (Mart 2019): 61-71. https://doi.org/10.24323/akademik-gida.544647.
EndNote Demir T, Akpınar Ö, Kara H, Güngör H (01 Mart 2019) Nar (Punica granatum L.) Kabuğunun İn Vitro Antidiyabetik, Antienflamatuar, Sitotoksik, Antioksidan ve Antimikrobiyal Aktivitesi. Akademik Gıda 17 1 61–71.
IEEE T. Demir, Ö. Akpınar, H. Kara, ve H. Güngör, “Nar (Punica granatum L.) Kabuğunun İn Vitro Antidiyabetik, Antienflamatuar, Sitotoksik, Antioksidan ve Antimikrobiyal Aktivitesi”, Akademik Gıda, c. 17, sy. 1, ss. 61–71, 2019, doi: 10.24323/akademik-gida.544647.
ISNAD Demir, Tuğba vd. “Nar (Punica Granatum L.) Kabuğunun İn Vitro Antidiyabetik, Antienflamatuar, Sitotoksik, Antioksidan Ve Antimikrobiyal Aktivitesi”. Akademik Gıda 17/1 (Mart 2019), 61-71. https://doi.org/10.24323/akademik-gida.544647.
JAMA Demir T, Akpınar Ö, Kara H, Güngör H. Nar (Punica granatum L.) Kabuğunun İn Vitro Antidiyabetik, Antienflamatuar, Sitotoksik, Antioksidan ve Antimikrobiyal Aktivitesi. Akademik Gıda. 2019;17:61–71.
MLA Demir, Tuğba vd. “Nar (Punica Granatum L.) Kabuğunun İn Vitro Antidiyabetik, Antienflamatuar, Sitotoksik, Antioksidan Ve Antimikrobiyal Aktivitesi”. Akademik Gıda, c. 17, sy. 1, 2019, ss. 61-71, doi:10.24323/akademik-gida.544647.
Vancouver Demir T, Akpınar Ö, Kara H, Güngör H. Nar (Punica granatum L.) Kabuğunun İn Vitro Antidiyabetik, Antienflamatuar, Sitotoksik, Antioksidan ve Antimikrobiyal Aktivitesi. Akademik Gıda. 2019;17(1):61-7.

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