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Propagation of GF 677 (P. amygdalus x P. persica) Clone Rootstock in Tissue Culture by Shoot-tip Techique

Year 2017, Volume: 54 Issue: 4, 447 - 451, 27.12.2017

Serra Hepaksoy

https://doi.org/10.20289/zfdergi.386559
Cited By: 3

Abstract





 



Modified Murashige and Skoog (MS) nutrient medium was
used to propagate the GF 677 clone rootstock by shoot tip technique.  Survival ratio of shoot tips was differed
between 38% and 60% and increased by BAP concentration. The best multiplication
was obtained on a MS medium supplemented with 2.0 mg/l BAP, 0.1 mg/l NAA and
0.1 mg/l GA3. In general multiplication ration was
increased when GA3 added to medium. Rooting of in vitro
plantlets was better with NAA than IBA while there was no significant
difference between auxins. The highest rooting was obtained on half or full
strength MS medium containing 1.0 and 1.5 mg/l NAA or IBA. Half or full
strength MS medium were showed similar  affect on rooting.

Keywords

GF 677 in vitro MS medium

References

  • Aghaye, M.N.R. and A. Yadollahi, 2012. Micropropagation of GF 677 rootstock. Journal of Agricultural Science, 4 (5): 131-138.
  • Ahmad, T., H.U. Rahman, C.H. Ahmad and M.H. Laghari, 2003. Effect of culture media and growth regulators on micropropagation of peach rootstock GF 677. Pakistan Journal of Botany, 35(3): 331–338.
  • Al-Tamimi M.O. and M.M. QrunBeh, 1996. Propagation of GF677 peach rootstock by stem cuttings. HortScience, 31(4).
  • Anonymous, 2017. Rahan Meristem (1998) Ltd, Plant Propagation & Biotechnology. http://www.rahan.co.il/?page_id=146&lang= en. Erişim: Şubat, 2017.
  • Antonopoulou, C., K. Dimassi, I. Therios, C. Chatzıssavidis and V. Tsirakoglou, 2005. Inhibitory effects of riboflavin (vitamin B2) on the ın vitro rooting and nutrient concentration of explants of peach rootstock GF 677 (P. amygdalus x P. persica). Biologia Plantarum, 48(4): 549-553.
  • Arıcı, Ş.E. 2008. Bazı sert çekirdekli meyve anaçlarının doku kültürü ile çoğaltılması. Süleyman Demirel Üniversitesi Ziraat Fakültesi Dergisi, 3 (1): 19-23.
  • Dakah, A., W. Mohsen and S. Zaid, 2014. Effect of nutrient media on in vitro micropropagation of GF-677 rootstock. Jordan Journal of Agricultural Sciences. 10 (3): 590-600.
  • Dimassi Theriou, K., 1995. In vitro rooting of rootstock GF-677 (P. amygdalus x P. persica) as influenced by mineral concentration of the nutrient medium and type of culture-tube sealing material. Journal of Horticultural Science, 70 (1): 105-108.
  • Dobránszki, J. and J.A. Teixeira da Silva, 2010. Micropropagation of apple – A review. Biotechnology Advances, 28, 462-488.
  • Malavasi, F.F.F. and R. Ranieri, 1987. Preliminary investigation on in vivo rooting of microcuttings of GF-677 peach rootstock. Acta Horticulturae 212: :281-288.
  • Hepaksoy, S. and U. Aksoy, 2006. Propagation of Ficus carica L. clones by in vitro culture. Biologia Plantarum, 50 (3): 433-436.
  • Hepaksoy, S. ve Ş. Kavaklı, 2016. GF 677 (Prunus amygdalus × P. persica) anacının odun çelikleriyle çoğaltılması. Bahçe 45 Özel sayı, Cilt 1: 985-990.
  • Jiangang, H. and G. Jisan, 1994. A study on tissue culture of Ficus carica L. Journal of Nanjing Forestry University, 18 (3): 73-76.
  • Kamali, K., E. Majidi and R. Zarghami, 2001. Micropropagation of GF- 677 roots tocks (Prunus amygdalus x P. persica). XI GREMPA Seminar on Pistachios and Almonds. CIHEAM, p. 175-177 (Cahiers Options Méditerranéennes. n. 56).
  • Küden, A.B., 2000. Şeftali Yetiştiriciliği. TÜBİTAK-TARP Yayınları, 20 s
  • Murashige, T. and F. Skoog, 1962. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plant. 15: 473-497.
  • Pevalek-Kozlina, B. and S. Jelaska, 1987. Microclonal propagation of Prunus avium L. Acta Horticulturae, 212: 599-602.
  • Rogalski, M., L.K.A.D. Moraes, C. Feslibino, L. Crestani, M.P. Guerra and A.L.D. Silva, 2003. In vitro rooting of prunus rootstocks. Brazil, Rev. Bras. Frutic., Jaboticabal - SP, 25 (2): 293-296.
  • Sutter, E.G., 1996. General laboratory requirements, media and sterilization methods. In Plant Tissue Culture Concepts and Laboratory Exercises. (Eds: R.N. Trigiano and D.J., Gray), CRC Press, New York, pp 11-25.
  • Stylinanides, D.C., Gr.C. Tsipouridis and Z.S. Michailidis, 1989. Resistance to iron deficiency of five peach rootstocks. Acta Horticulturae, 254:185-188.
  • Tsipouridis, C., T. Thomidis and S. Bladenopoulou, 2006. Seasonal variation in sproutig of GF 677 peach x almond (Prunus persica x Prunus amygdalus) hybrid root cutting. New Zealand Journal of Crop and Horticultural Science, 34: 45-50.

GF 677 (P. amygdalus x P. persica) Klon Anacının Doku Kültüründe Sürgünucu Tekniği ile Çoğaltılması

Year 2017, Volume: 54 Issue: 4, 447 - 451, 27.12.2017

Serra Hepaksoy

https://doi.org/10.20289/zfdergi.386559
Cited By: 3

Abstract





 



GF  677
klon anacının sürgün ucu tekniğiyle çoğaltılması amacıyla modifiye Murashige
Skoog (MS) besin ortamı kullanılmıştır. Sürgün uçlarının canlılık oranı %38 ile
% 60 arasında değişmiş ve BAP konsantrasyonu arttıkça canlılık oranı da
artmıştır. En iyi çoğalma 2.0 mg/l BAP + 0.1 mg/l NAA + 0.1 mg/l GA3
içeren MS ortamından elde edilmiş, genel olarak besin ortamında GA3
bulunmasının kardeşlenme sayısını arttırdığı tespit edilmiştir. In vitro bitkiciklerin köklenmesinde
NAA, IBA’e göre daha iyi sonuç vermekle birlikte, istatistiksel olarak önemli
farklılıklar bulunmamıştır. Besin ortamına 1.0 ve 1.5 mg/l NAA veya IBA ilave
edilmesi en yüksek köklenme oranlarını vermiştir. Tam veya yarı kuvvette MS
besin ortamının kullanılması köklenme üzerine benzer etki göstermiştir.

Keywords

GF 677 in vitro MS ortamı

References

  • Aghaye, M.N.R. and A. Yadollahi, 2012. Micropropagation of GF 677 rootstock. Journal of Agricultural Science, 4 (5): 131-138.
  • Ahmad, T., H.U. Rahman, C.H. Ahmad and M.H. Laghari, 2003. Effect of culture media and growth regulators on micropropagation of peach rootstock GF 677. Pakistan Journal of Botany, 35(3): 331–338.
  • Al-Tamimi M.O. and M.M. QrunBeh, 1996. Propagation of GF677 peach rootstock by stem cuttings. HortScience, 31(4).
  • Anonymous, 2017. Rahan Meristem (1998) Ltd, Plant Propagation & Biotechnology. http://www.rahan.co.il/?page_id=146&lang= en. Erişim: Şubat, 2017.
  • Antonopoulou, C., K. Dimassi, I. Therios, C. Chatzıssavidis and V. Tsirakoglou, 2005. Inhibitory effects of riboflavin (vitamin B2) on the ın vitro rooting and nutrient concentration of explants of peach rootstock GF 677 (P. amygdalus x P. persica). Biologia Plantarum, 48(4): 549-553.
  • Arıcı, Ş.E. 2008. Bazı sert çekirdekli meyve anaçlarının doku kültürü ile çoğaltılması. Süleyman Demirel Üniversitesi Ziraat Fakültesi Dergisi, 3 (1): 19-23.
  • Dakah, A., W. Mohsen and S. Zaid, 2014. Effect of nutrient media on in vitro micropropagation of GF-677 rootstock. Jordan Journal of Agricultural Sciences. 10 (3): 590-600.
  • Dimassi Theriou, K., 1995. In vitro rooting of rootstock GF-677 (P. amygdalus x P. persica) as influenced by mineral concentration of the nutrient medium and type of culture-tube sealing material. Journal of Horticultural Science, 70 (1): 105-108.
  • Dobránszki, J. and J.A. Teixeira da Silva, 2010. Micropropagation of apple – A review. Biotechnology Advances, 28, 462-488.
  • Malavasi, F.F.F. and R. Ranieri, 1987. Preliminary investigation on in vivo rooting of microcuttings of GF-677 peach rootstock. Acta Horticulturae 212: :281-288.
  • Hepaksoy, S. and U. Aksoy, 2006. Propagation of Ficus carica L. clones by in vitro culture. Biologia Plantarum, 50 (3): 433-436.
  • Hepaksoy, S. ve Ş. Kavaklı, 2016. GF 677 (Prunus amygdalus × P. persica) anacının odun çelikleriyle çoğaltılması. Bahçe 45 Özel sayı, Cilt 1: 985-990.
  • Jiangang, H. and G. Jisan, 1994. A study on tissue culture of Ficus carica L. Journal of Nanjing Forestry University, 18 (3): 73-76.
  • Kamali, K., E. Majidi and R. Zarghami, 2001. Micropropagation of GF- 677 roots tocks (Prunus amygdalus x P. persica). XI GREMPA Seminar on Pistachios and Almonds. CIHEAM, p. 175-177 (Cahiers Options Méditerranéennes. n. 56).
  • Küden, A.B., 2000. Şeftali Yetiştiriciliği. TÜBİTAK-TARP Yayınları, 20 s
  • Murashige, T. and F. Skoog, 1962. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plant. 15: 473-497.
  • Pevalek-Kozlina, B. and S. Jelaska, 1987. Microclonal propagation of Prunus avium L. Acta Horticulturae, 212: 599-602.
  • Rogalski, M., L.K.A.D. Moraes, C. Feslibino, L. Crestani, M.P. Guerra and A.L.D. Silva, 2003. In vitro rooting of prunus rootstocks. Brazil, Rev. Bras. Frutic., Jaboticabal - SP, 25 (2): 293-296.
  • Sutter, E.G., 1996. General laboratory requirements, media and sterilization methods. In Plant Tissue Culture Concepts and Laboratory Exercises. (Eds: R.N. Trigiano and D.J., Gray), CRC Press, New York, pp 11-25.
  • Stylinanides, D.C., Gr.C. Tsipouridis and Z.S. Michailidis, 1989. Resistance to iron deficiency of five peach rootstocks. Acta Horticulturae, 254:185-188.
  • Tsipouridis, C., T. Thomidis and S. Bladenopoulou, 2006. Seasonal variation in sproutig of GF 677 peach x almond (Prunus persica x Prunus amygdalus) hybrid root cutting. New Zealand Journal of Crop and Horticultural Science, 34: 45-50.
There are 21 citations in total.

Details

Subjects Engineering
Journal Section Articles
Authors

Serra Hepaksoy

Publication Date December 27, 2017
Submission Date March 6, 2017
Acceptance Date May 15, 2017
Published in Issue Year 2017 Volume: 54 Issue: 4

Cite

APA Hepaksoy, S. (2017). GF 677 (P. amygdalus x P. persica) Klon Anacının Doku Kültüründe Sürgünucu Tekniği ile Çoğaltılması. Ege Üniversitesi Ziraat Fakültesi Dergisi, 54(4), 447-451. https://doi.org/10.20289/zfdergi.386559

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https://doi.org/10.1007/s10341-023-00908-6
Effects of charcoal and physical state of medium on micropropagation of black mulbery (Morus nigra L.)
Ege Üniversitesi Ziraat Fakültesi Dergisi
https://doi.org/10.20289/zfdergi.1198529
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