BibTex RIS Cite

GENOTOXICITY OF IRON OXIDE NANOPARTICLES IN HUMAN LYMPHOCYTES BY SISTER CHROMATID EXCHANGES

Year 2017, Volume: Volume 2 Issue: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology, 186 - 186, 16.02.2017

Abstract

In recent years,
iron oxide nanoparticles (Fe2O3 NPs) have been widely
used in biomedical applications, for both diagnosis and therapy, due to their
unique magnetic properties. Although the wide spread using, the impact of Fe2O3
NPs on the environment and on biological species is not well understood. In
this study, genotoxic effects of Fe2O3 NPs have been
determined in human peripheral lymphocytes in vitro by using sister chromatid
exchange (SCEs) assay. Peripheral lymphocytes were incubated with four different
concentrations (39,062; 78,125; 156,25 and 312,5 μg/mL) of Fe2O3
NPs (Sigma-Aldrich, <50 nm, spherical) for 24 and 48h. The results showed
that the frequency of SCEs increased at all the treatment times dose
dependently (r=0,85 for 24h; r=0,86 for 48 h), however, this increase was
significant only at the highest concentration (312,500 μg/mL) at 24 h, and at
the two highest concentrations (156,250 and 312,500 μg/mL) at 48h. The
formation of SCEs has been correlated with recombinational repair and the induction
of point mutations, gene amplification and cytotoxicity. This effect may occur
from oxidative stress that has been produced by reactive oxygen species (ROS).
Long term exposure to these nanoparticles could potentially result in particle
accumulation and subsequently induce acute or chronic toxicity. This may
provoke a range of long term effect involving mutagenic, carcinogenic or
teratogenic influence on the organism.

References

  • Yasemin SAYGILI, Fatma ÜNAL, Deniz YÜZBAŞIOĞLU
  • Genetic Toxicology Laboratory, Department of Biology, Faculty of Science, Gazi University, Ankara-Turkey
Year 2017, Volume: Volume 2 Issue: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology, 186 - 186, 16.02.2017

Abstract

References

  • Yasemin SAYGILI, Fatma ÜNAL, Deniz YÜZBAŞIOĞLU
  • Genetic Toxicology Laboratory, Department of Biology, Faculty of Science, Gazi University, Ankara-Turkey
There are 2 citations in total.

Details

Journal Section Articles
Authors

Yasemin Saygılı This is me

Fatma Ünal

Deniz Yüzbaşıoğlu This is me

Publication Date February 16, 2017
Published in Issue Year 2017 Volume: Volume 2 Issue: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology

Cite

APA Saygılı, Y., Ünal, F., & Yüzbaşıoğlu, D. (2017). GENOTOXICITY OF IRON OXIDE NANOPARTICLES IN HUMAN LYMPHOCYTES BY SISTER CHROMATID EXCHANGES. The Turkish Journal Of Occupational / Environmental Medicine and Safety, Volume 2(İssue 1 (1), 186-186.
AMA Saygılı Y, Ünal F, Yüzbaşıoğlu D. GENOTOXICITY OF IRON OXIDE NANOPARTICLES IN HUMAN LYMPHOCYTES BY SISTER CHROMATID EXCHANGES. turjoem. February 2017;Volume 2(İssue 1 (1):186-186.
Chicago Saygılı, Yasemin, Fatma Ünal, and Deniz Yüzbaşıoğlu. “GENOTOXICITY OF IRON OXIDE NANOPARTICLES IN HUMAN LYMPHOCYTES BY SISTER CHROMATID EXCHANGES”. The Turkish Journal Of Occupational / Environmental Medicine and Safety Volume 2, no. İssue 1 (1) (February 2017): 186-86.
EndNote Saygılı Y, Ünal F, Yüzbaşıoğlu D (February 1, 2017) GENOTOXICITY OF IRON OXIDE NANOPARTICLES IN HUMAN LYMPHOCYTES BY SISTER CHROMATID EXCHANGES. The Turkish Journal Of Occupational / Environmental Medicine and Safety Volume 2 İssue 1 (1) 186–186.
IEEE Y. Saygılı, F. Ünal, and D. Yüzbaşıoğlu, “GENOTOXICITY OF IRON OXIDE NANOPARTICLES IN HUMAN LYMPHOCYTES BY SISTER CHROMATID EXCHANGES”, turjoem, vol. Volume 2, no. İssue 1 (1), pp. 186–186, 2017.
ISNAD Saygılı, Yasemin et al. “GENOTOXICITY OF IRON OXIDE NANOPARTICLES IN HUMAN LYMPHOCYTES BY SISTER CHROMATID EXCHANGES”. The Turkish Journal Of Occupational / Environmental Medicine and Safety VOLUME 2/İssue 1 (1) (February 2017), 186-186.
JAMA Saygılı Y, Ünal F, Yüzbaşıoğlu D. GENOTOXICITY OF IRON OXIDE NANOPARTICLES IN HUMAN LYMPHOCYTES BY SISTER CHROMATID EXCHANGES. turjoem. 2017;Volume 2:186–186.
MLA Saygılı, Yasemin et al. “GENOTOXICITY OF IRON OXIDE NANOPARTICLES IN HUMAN LYMPHOCYTES BY SISTER CHROMATID EXCHANGES”. The Turkish Journal Of Occupational / Environmental Medicine and Safety, vol. Volume 2, no. İssue 1 (1), 2017, pp. 186-.
Vancouver Saygılı Y, Ünal F, Yüzbaşıoğlu D. GENOTOXICITY OF IRON OXIDE NANOPARTICLES IN HUMAN LYMPHOCYTES BY SISTER CHROMATID EXCHANGES. turjoem. 2017;Volume 2(İssue 1 (1):186-.