Research Article
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Amanita vidua: A new record for Turkish Amanita Section Phalloideae based on morphological and molecular data

Year 2024, Volume: 25 Issue: 1, 97 - 110, 15.04.2024

Ilgaz Akata Eda Kumru Ergin Sahin İsmail Acar Ertuğrul Kaya

https://doi.org/10.23902/trkjnat.1446327

Abstract

In the present study, fungal specimens collected from Yeşildere village in Uşak province, Türkiye were evaluated. The specimens underwent detailed examination through both mor-phological and DNA (nrITS rDNA region and TEF1a gene) sequence-based phylogenetic analysis. Based on the evaluation of micro- and macromorphological characteristics, and phylogenetic analyses, the specimen was identified as A. vidua Gasch, G. Moreno & P.-A. Moreau. This identification marks A. vidua as a new record for the Turkish Amanita section Phalloideae. The study described this newly recorded species, including specific details such as its location, habitat observations, geographical coordinates, collection date, and pho-tographs showcasing its macro and micromorphological features. Additionally, it also in-cludes illustrations of some microscopic structures of the species. The study is further en-riched with SEM images, offering a detailed view of the species characteristics, and the findings are briefly discussed.

Keywords

Amanitaceae Mycobiota Asia Minor Türkiye

Ethical Statement

Since the article does not contain any studies with human or animal subject, its approval to the ethics committee was not required.

Supporting Institution

Ankara University Research Funding Unit

Project Number

FLO-2022-2709

Thanks

Special thanks to Meltem Kurt (Türkiye) for her invaluable contribution to the project. Her dedication and expertise were instrumental in the detailed illustration of fresh specimens of acorns and a leaf of the Valonia oak, as well as the basidiomata of newly collected Amanita vidua specimens.

References

  • 1. Akata, I., Kaya E., Yılmaz, İ., Bakırcı S. & Bayram, R. 2015. Türkiye’de Yetişen Alfa Amanitin İçeren Mantarlar. Düzce Tıp Fakültesi Dergisi, 17(1): 39-44.
  • 2. Alvarado, P., Gasch-Illescas, A., Morel, S., Dagher-Kharrat, M.B. Moreno, G., Manjón, J.L., Carteret, X., Bellanger, J.M., Rapior, S., Gelardi, M. & Moreau P.A. 2022. Amanita Section Phalloideae Species in the Mediterranean Basin: Destroying Angels Reviewed. Biology, 11: 770. https://doi.org/10.3390/biology11050770
  • 3. Chen, L., Cai, Y., Zhou, G., Shi, X., Su, J., Chen, G. & Lin, K. 2014. Rapid Sanger sequencing of the 16S rRNA gene for identification of some common pathogens. PloS one, 9(2): e88886. https://doi.org/10.1371/journal.pone.0088886
  • 4. Codjia, J.E.I., Cai, Q., Zhou, S.W., Lou, H., Ryberg, M., Yorou, N.S. & Yang, Z.L. 2020. Morphology, multilocus phylogeny, and toxin analysis reveal Amanita albolimbata, the first lethal Amanita species from Benin, West Africa. Frontiers in Microbiology, 11: 599047. https://doi.org/10.3389/fmicb.2020.599047
  • 5. Codjia, J.E.I., Wang, P.M., Ryberg, M., Yorou, N.S. & Yang, Z.L. 2022. Amanita sect. Phalloideae: two interesting non-lethal species from West Africa. Mycological Progress, 21(3): 21-39. https://doi.org/10.1007/s11557-022-01778-0
  • 6. Corrales, A., Henkel, T.W. & Smith, M.E. 2018. Ectomycorrhizal associations in the tropics – biogeography, diversity patterns, and ecosystem roles. New Phytologist, 220: 1076-1091. https://doi.org/10.1111/nph.15151
  • 7. Cui, Y.Y., Cai, Q., Tang, L.P., Liu, J.W. & Yang, Z.L. 2018. The family Amanitaceae: molecular phylogeny, higher-rank taxonomy and the species in China. Fungal Diversity, 91: 5-230. https://doi.org/10.1007/s13225-018-0405-9
  • 8. Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution, 39(4): 783-791.
  • 9. Fraiture, A., Amalfi, M., Raspé, O., Kaya, E., Akata, I. & Degreef, J. 2019. Two new species of Amanita sect. Phalloideae from Africa, one of which is devoid of amatoxins and phallotoxins. MycoKeys, 53: 93-125. https://doi.org/10.3897/mycokeys.53.34560
  • 10. Kumar, S., Stecher, G., Li, M., Knyaz, C. & Tamura, K. 2018. MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 35(6): 1547-1549. https://doi.org/10.1093/molbev/msy096
  • 11. Kimura, M. 1980. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16: 111-120. https://doi.org/10.1007/BF01731581
  • 12. Martin, K.J. & Rygiewicz, P.T. 2005. Fungal-specific PCR primers developed for analysis of the ITS region of environmental DNA extracts. BMC Microbiology, 5(28): 1-12. https://doi.org/10.1186/1471-2180-5-28
  • 13. Raja, H.A., Miller, A.N., Pearce, C.J. & Oberlies, N.H. 2017. Fungal identification using molecular tools: a primer for the natural products research community. Journal of Natural Products, 80(3): 756-770. https://doi.org/10.1021/acs.jnatprod.6b01085
  • 14. Rehner, S.A. & Buckley, E. 2005. A Beauveria phylogeny inferred from nuclear ITS and EF1-α sequences: evidence for cryptic diversification and links to Cordyceps teleomorphs. Mycologia, 97: 84-98. https://doi.org/10.3852/mycologia.97.1.84
  • 15. Rogers, S.O. & Bendich, A.J. 1994. Extraction of total cellular DNA from plants, algae and fungi. pp. 183-190. In: Gelvin, S.B. & Schilperoort, R.A. (eds). Plant Molecular Biology Manual. Springer Netherlands, Dordrecht, 598 pp.
  • 16. Sesli, E. 2022. Amanita spadicea: Türkiye Mikotası İçin Yeni Bir Kayıt. Mantar Dergisi, 13(2): 92-95. https://doi.org/10.30708.mantar.1094745
  • 17. Sesli, E., Asan, A., Selçuk, F., Arabacı Günyar, Ö., Akata, I., Akgül, H., Aktaş, S., Alkan, S., Allı, H., Aydoğdu, H., Berikten, D., Demirel, K., Demirel, R., Doğan, H.H., Erdoğdu, M., Ergül, C.C., Eroğlu, G., Giray, G.., Halik Uztan, A., Kabaktepe, Ş., Kadaifçiler, D., Kalyoncu, F., Karaltı, İ., Kaşık, G., Kaya, A., Keleş, A., Kırbağ, S., Kıvanç, M., Ocak, İ., Ökten, S., Özkale, E., Öztürk, C., Sevindik, M., Şen, B., Şen, İ., Türkekul, İ., Ulukapı, M., Uzun, Ya., Uzun, Yu. & Yoltaş, A. 2020. Türkiye Mantarları Listesi. Ali Nihat Gökyiğit Vakfı Yayını, İstanbul, 1177 pp.
  • 18. Tamura, K. 1992. Estimation of the number of nucleotide substitutions when there are strong transition-transversion and G + C-content biases. Molecular Biology and Evolution, 9: 678-687. https://doi.org/10.1093/oxfordjournals.molbev.a040752
  • 19. Thongbai, B., Miller. S.L., Stadler, M., Wittstein, K., Hyde, K.D., Lumyong, S. & Raspe, O. 2017. Study of three interesting Amanita species from Thailand: Morphology, multiple-gene phylogeny, and toxin analysis. PloS one, 12(8): e0182131. https://doi.org/10.1371/journal.pone.0182131
  • 20. White, T.J., Bruns, TD, Lee, S. & Taylor, J.W. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. pp. 315-322. In: Innis, M.A. & Gelfand, D.H. (eds). PCR Protocols: A Guide to Methods and Applications. Academic Press, London, 482 pp.

Year 2024, Volume: 25 Issue: 1, 97 - 110, 15.04.2024

Ilgaz Akata Eda Kumru Ergin Sahin İsmail Acar Ertuğrul Kaya

https://doi.org/10.23902/trkjnat.1446327

Abstract

Bu çalışmada Türkiye'nin Uşak iline bağlı Yeşildere Köyü’nden toplanan mantar örnekleri değerlendirilmiştir. Bu örnekler hem morfolojik hem de DNA (nrITS rDNA bölgesi ve TEF1a geni) dizisine dayalı filogenetik analiz yoluyla ayrıntılı incelemeye tabi tutulmuştur. Mikro ve makromorfolojik özelliklerin değerlendirilmesi ve filogenetik analizlere dayanarak örnek A. vidua Gasch, G. Moreno & P.-A. Moreau olarak tanımlanmıştır. Bu tanımlama, A. vidua' yı Türkiye’deki Amanita cinsinin Phalloideae seksyonu için yeni bir kayıt olarak be-lirlenmiştir. Çalışma, yeni kaydedilen bu türün konumu, habitat gözlemleri, coğrafi koordi-natları, toplanma tarihi ve makro ve mikromorfolojik özelliklerini gösteren fotoğraflar gibi belirli ayrıntıları içeren bir tanımını sunmaktadır. Ayrıca türün bazı mikroskobik yapılarına ait çizimler de yer almaktadır. Çalışma, türün karmaşık özelliklerinin ayrıntılı bir görünü-münü sunan taramalı elektron mikroskobundan (SEM) elde edilen görüntülerle daha da zen-ginleştirilmiştir ve bu bulgular kısaca tartışılmıştır.

Project Number

FLO-2022-2709

References

  • 1. Akata, I., Kaya E., Yılmaz, İ., Bakırcı S. & Bayram, R. 2015. Türkiye’de Yetişen Alfa Amanitin İçeren Mantarlar. Düzce Tıp Fakültesi Dergisi, 17(1): 39-44.
  • 2. Alvarado, P., Gasch-Illescas, A., Morel, S., Dagher-Kharrat, M.B. Moreno, G., Manjón, J.L., Carteret, X., Bellanger, J.M., Rapior, S., Gelardi, M. & Moreau P.A. 2022. Amanita Section Phalloideae Species in the Mediterranean Basin: Destroying Angels Reviewed. Biology, 11: 770. https://doi.org/10.3390/biology11050770
  • 3. Chen, L., Cai, Y., Zhou, G., Shi, X., Su, J., Chen, G. & Lin, K. 2014. Rapid Sanger sequencing of the 16S rRNA gene for identification of some common pathogens. PloS one, 9(2): e88886. https://doi.org/10.1371/journal.pone.0088886
  • 4. Codjia, J.E.I., Cai, Q., Zhou, S.W., Lou, H., Ryberg, M., Yorou, N.S. & Yang, Z.L. 2020. Morphology, multilocus phylogeny, and toxin analysis reveal Amanita albolimbata, the first lethal Amanita species from Benin, West Africa. Frontiers in Microbiology, 11: 599047. https://doi.org/10.3389/fmicb.2020.599047
  • 5. Codjia, J.E.I., Wang, P.M., Ryberg, M., Yorou, N.S. & Yang, Z.L. 2022. Amanita sect. Phalloideae: two interesting non-lethal species from West Africa. Mycological Progress, 21(3): 21-39. https://doi.org/10.1007/s11557-022-01778-0
  • 6. Corrales, A., Henkel, T.W. & Smith, M.E. 2018. Ectomycorrhizal associations in the tropics – biogeography, diversity patterns, and ecosystem roles. New Phytologist, 220: 1076-1091. https://doi.org/10.1111/nph.15151
  • 7. Cui, Y.Y., Cai, Q., Tang, L.P., Liu, J.W. & Yang, Z.L. 2018. The family Amanitaceae: molecular phylogeny, higher-rank taxonomy and the species in China. Fungal Diversity, 91: 5-230. https://doi.org/10.1007/s13225-018-0405-9
  • 8. Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution, 39(4): 783-791.
  • 9. Fraiture, A., Amalfi, M., Raspé, O., Kaya, E., Akata, I. & Degreef, J. 2019. Two new species of Amanita sect. Phalloideae from Africa, one of which is devoid of amatoxins and phallotoxins. MycoKeys, 53: 93-125. https://doi.org/10.3897/mycokeys.53.34560
  • 10. Kumar, S., Stecher, G., Li, M., Knyaz, C. & Tamura, K. 2018. MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 35(6): 1547-1549. https://doi.org/10.1093/molbev/msy096
  • 11. Kimura, M. 1980. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16: 111-120. https://doi.org/10.1007/BF01731581
  • 12. Martin, K.J. & Rygiewicz, P.T. 2005. Fungal-specific PCR primers developed for analysis of the ITS region of environmental DNA extracts. BMC Microbiology, 5(28): 1-12. https://doi.org/10.1186/1471-2180-5-28
  • 13. Raja, H.A., Miller, A.N., Pearce, C.J. & Oberlies, N.H. 2017. Fungal identification using molecular tools: a primer for the natural products research community. Journal of Natural Products, 80(3): 756-770. https://doi.org/10.1021/acs.jnatprod.6b01085
  • 14. Rehner, S.A. & Buckley, E. 2005. A Beauveria phylogeny inferred from nuclear ITS and EF1-α sequences: evidence for cryptic diversification and links to Cordyceps teleomorphs. Mycologia, 97: 84-98. https://doi.org/10.3852/mycologia.97.1.84
  • 15. Rogers, S.O. & Bendich, A.J. 1994. Extraction of total cellular DNA from plants, algae and fungi. pp. 183-190. In: Gelvin, S.B. & Schilperoort, R.A. (eds). Plant Molecular Biology Manual. Springer Netherlands, Dordrecht, 598 pp.
  • 16. Sesli, E. 2022. Amanita spadicea: Türkiye Mikotası İçin Yeni Bir Kayıt. Mantar Dergisi, 13(2): 92-95. https://doi.org/10.30708.mantar.1094745
  • 17. Sesli, E., Asan, A., Selçuk, F., Arabacı Günyar, Ö., Akata, I., Akgül, H., Aktaş, S., Alkan, S., Allı, H., Aydoğdu, H., Berikten, D., Demirel, K., Demirel, R., Doğan, H.H., Erdoğdu, M., Ergül, C.C., Eroğlu, G., Giray, G.., Halik Uztan, A., Kabaktepe, Ş., Kadaifçiler, D., Kalyoncu, F., Karaltı, İ., Kaşık, G., Kaya, A., Keleş, A., Kırbağ, S., Kıvanç, M., Ocak, İ., Ökten, S., Özkale, E., Öztürk, C., Sevindik, M., Şen, B., Şen, İ., Türkekul, İ., Ulukapı, M., Uzun, Ya., Uzun, Yu. & Yoltaş, A. 2020. Türkiye Mantarları Listesi. Ali Nihat Gökyiğit Vakfı Yayını, İstanbul, 1177 pp.
  • 18. Tamura, K. 1992. Estimation of the number of nucleotide substitutions when there are strong transition-transversion and G + C-content biases. Molecular Biology and Evolution, 9: 678-687. https://doi.org/10.1093/oxfordjournals.molbev.a040752
  • 19. Thongbai, B., Miller. S.L., Stadler, M., Wittstein, K., Hyde, K.D., Lumyong, S. & Raspe, O. 2017. Study of three interesting Amanita species from Thailand: Morphology, multiple-gene phylogeny, and toxin analysis. PloS one, 12(8): e0182131. https://doi.org/10.1371/journal.pone.0182131
  • 20. White, T.J., Bruns, TD, Lee, S. & Taylor, J.W. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. pp. 315-322. In: Innis, M.A. & Gelfand, D.H. (eds). PCR Protocols: A Guide to Methods and Applications. Academic Press, London, 482 pp.
There are 20 citations in total.

Details

Primary Language English
Subjects Mycology
Journal Section Research Article/Araştırma Makalesi
Authors

Ilgaz Akata 0000-0002-1731-1302

Eda Kumru 0009-0000-7417-6197

Ergin Sahin 0000-0003-1711-738X

İsmail Acar This is me 0000-0002-6049-4896

Ertuğrul Kaya 0000-0003-0081-682X

Project Number FLO-2022-2709
Early Pub Date April 9, 2024
Publication Date April 15, 2024
Submission Date March 2, 2024
Acceptance Date March 30, 2024
Published in Issue Year 2024 Volume: 25 Issue: 1

Cite

APA Akata, I., Kumru, E., Sahin, E., Acar, İ., et al. (2024). Amanita vidua: A new record for Turkish Amanita Section Phalloideae based on morphological and molecular data. Trakya University Journal of Natural Sciences, 25(1), 97-110. https://doi.org/10.23902/trkjnat.1446327
AMA Akata I, Kumru E, Sahin E, Acar İ, Kaya E. Amanita vidua: A new record for Turkish Amanita Section Phalloideae based on morphological and molecular data. Trakya Univ J Nat Sci. April 2024;25(1):97-110. doi:10.23902/trkjnat.1446327
Chicago Akata, Ilgaz, Eda Kumru, Ergin Sahin, İsmail Acar, and Ertuğrul Kaya. “Amanita Vidua: A New Record for Turkish Amanita Section Phalloideae Based on Morphological and Molecular Data”. Trakya University Journal of Natural Sciences 25, no. 1 (April 2024): 97-110. https://doi.org/10.23902/trkjnat.1446327.
EndNote Akata I, Kumru E, Sahin E, Acar İ, Kaya E (April 1, 2024) Amanita vidua: A new record for Turkish Amanita Section Phalloideae based on morphological and molecular data. Trakya University Journal of Natural Sciences 25 1 97–110.
IEEE I. Akata, E. Kumru, E. Sahin, İ. Acar, and E. Kaya, “Amanita vidua: A new record for Turkish Amanita Section Phalloideae based on morphological and molecular data”, Trakya Univ J Nat Sci, vol. 25, no. 1, pp. 97–110, 2024, doi: 10.23902/trkjnat.1446327.
ISNAD Akata, Ilgaz et al. “Amanita Vidua: A New Record for Turkish Amanita Section Phalloideae Based on Morphological and Molecular Data”. Trakya University Journal of Natural Sciences 25/1 (April 2024), 97-110. https://doi.org/10.23902/trkjnat.1446327.
JAMA Akata I, Kumru E, Sahin E, Acar İ, Kaya E. Amanita vidua: A new record for Turkish Amanita Section Phalloideae based on morphological and molecular data. Trakya Univ J Nat Sci. 2024;25:97–110.
MLA Akata, Ilgaz et al. “Amanita Vidua: A New Record for Turkish Amanita Section Phalloideae Based on Morphological and Molecular Data”. Trakya University Journal of Natural Sciences, vol. 25, no. 1, 2024, pp. 97-110, doi:10.23902/trkjnat.1446327.
Vancouver Akata I, Kumru E, Sahin E, Acar İ, Kaya E. Amanita vidua: A new record for Turkish Amanita Section Phalloideae based on morphological and molecular data. Trakya Univ J Nat Sci. 2024;25(1):97-110.

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