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Biosynthesis and In Vitro Antioxidant Activities of Zinc Oxide Nanoparticles with Turkish oregano (Origanum onites L.) Extract

Year 2023, Volume: 6 Issue: 3, 150 - 155, 31.12.2023
https://doi.org/10.56150/tjhsl.1391498

Abstract

Plant-mediated biosynthesis of metallic nanoparticles (NPs) occurs via biomolecules containing organic functional groups found in the plant. The aim of the study was to characterize and evaluate the antioxidant properties of zinc oxide nanoparticles (ZnO NPs) synthesized from Turkish oregano (Origanum onites L.), a traditional endemic plant. Some characteristic properties of ZnO NPs were determined by using the methods that ultraviolet visible spectroscopy (UV–Vis), scanning electron microscopy (SEM), Energy Dispersive X-ray analysis (EDX) and X-ray diffraction (XRD). UV–vis absorption peak of ZnO NPs observed at about 356 nm. Spherical shaped and nano-sized Zn ONPs has been detected by SEM. EDX analysis revealed that zinc is the primary constituent (50.69 %) with strong peaks at 1 keV. The average crystallite size of ZnO NPs has been showed at 30 nm by x-ray diffractometer. Antioxidant activity was investigated using a DPPH free radical quenching assay ZnO NPs demonstrated moderate antioxidant activity by scavenging 42.07%. Ultimately, the study shows that phyto-fabricated ZnO NPs were successfully synthesized and have a reasonable antioxidant effect.

References

  • Geetha, K., Umadevi, M., Sathe, G. V., & Erenler, R. Spectroscopic investigations on the orientation of 1, 4-dibromonaphthalene on silver nanoparticles. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 116, (2013), 236-241. https://doi: 10.1016/j.saa.2013.07.039
  • Burlec, A. F., Corciova, A., Boev, M., Batir-Marin, D., Mircea, C., Cioanca, O., ... & Hancianu, M. Current Overview of Metal Nanoparticles’ Synthesis, Characterization, and Biomedical Applications, with a Focus on Silver and Gold Nanoparticles. Pharmaceuticals, 16(10), (2023), 1410. https://doi.org/10.3390/ph16101410
  • Sutradhar, P., & Saha, M. Green synthesis of zinc oxide nanoparticles using tomato (Lycopersicon esculentum) extract and its photovoltaic application. Journal of Experimental Nanoscience, 11(5), (2016), 314-327.
  • Erdoğan, Ö., Birtekocak, F., Oryaşın, E., Abbak, M., Demirbolat, G. M., Salih, P. A. Ş. A., & Çevik, Ö. Enginar yaprağı sulu ekstraktı kullanılarak çinko oksit nanopartiküllerinin yeşil sentezi, karakterizasyonu, anti-bakteriyel ve sitotoksik etkileri. Duzce Medical Journal, 21(1), (2019), 19-26.
  • Parveen, K., Banse, V., & Ledwani, L. (2016, April). Green synthesis of nanoparticles: Their advantages and disadvantages. In AIP conference proceedings (Vol. 1724, No. 1). AIP Publishing.
  • Salam, H. A., Sivaraj, R., & Venckatesh, R. Green synthesis and characterization of zinc oxide nanoparticles from Ocimum basilicum L. var. purpurascens Benth.-Lamiaceae leaf extract. Materials letters, 131, (2014), 16-18.
  • Rajendran, S. P., & Sengodan, K. Synthesis and characterization of zinc oxide and iron oxide nanoparticles using Sesbania grandiflora leaf extract as reducing agent. Journal of Nanoscience, 2017.
  • Yuvakkumar, R., Suresh, J., Nathanael, A. J., Sundrarajan, M., & Hong, S. I. Novel green synthetic strategy to prepare ZnO nanocrystals using rambutan (Nephelium lappaceum L.) peel extract and its antibacterial applications. Materials Science and Engineering: C, 41, (2014), 17-27.
  • Agarwal, H., Kumar, S. V., & Rajeshkumar, S. A review on green synthesis of zinc oxide nanoparticles–An eco-friendly approach. Resource-Efficient Technologies, 3(4), (2017), 406-413.
  • 1Zuhrotun, A., Oktaviani, D. J., & Hasanah, A. N. Biosynthesis of Gold and Silver Nanoparticles Using Phytochemical Compounds. Molecules, 28(7), (2023), 3240.
  • Erenler, R., Tugba, A. D. A. K., Karan, T., Elmastas, M., Yildiz, I., Aksit, H., ... & Sanda, M. A. Chemical constituents isolated from Origanum solymicum with antioxidant activitites. The Eurasia Proceedings of Science Technology Engineering and Mathematics, (1), (2017), 139-145.
  • Tepe, B., Cakir, A., & Sihoglu Tepe, A. Medicinal uses, phytochemistry, and pharmacology of Origanum onites (L.): A Review. Chemistry & Biodiversity, 13(5), (2016), 504-520.
  • Erenler, R., Meral, B., Sen, O., Elmastas, M., Aydin, A., Eminagaoglu, O., & Topcu, G. Bioassay-guided isolation, identification of compounds from Origanum rotundifolium and investigation of their antiproliferative and antioxidant activities. Pharmaceutical Biology, 55(1), (2017), 1646-1653.
  • Erenler, R., Demirtas, I., Karan, T., Gul, F., Kayir, O., & Karakoc, O. C. Chemical constituents, quantitative analysis and insecticidal activities of plant extract and essential oil from Origanum onites L. Trends in Phytochemical Research, 2(2), (2018), 91-96.
  • Ozkan, G., Baydar, H., & Erbas, S. The influence of harvest time on essential oil composition, phenolic constituents and antioxidant properties of Turkish oregano (Origanum onites L.). Journal of the Science of Food and Agriculture, 90(2), (2010), 205-209.
  • Pehlivanoglu, S., Acar, C. A., & Donmez, S. Characterization of green synthesized flaxseed zinc oxide nanoparticles and their cytotoxic, apoptotic and antimigratory activities on aggressive human cancer cells. Inorganic and Nano-Metal Chemistry, (2021), 1-10.
  • Aydin Acar, C., Gencer, M. A., Pehlivanoglu, S., Yesilot, S., & Donmez, S. Green and eco‐friendly biosynthesis of zinc oxide nanoparticles using Calendula officinalis flower extract: Wound healing potential and antioxidant activity. International Wound Journal, (2023) https://doi.org/10.1111/iwj.14413.
  • Donmez, S. & Keyvan, E. Green synthesis of zinc oxide nanoparticles using grape seed extract and evaluation of their antibacterial and antioxidant activities, Inorganic and Nano-Metal Chemistry, (2023), DOI: 10.1080/24701556.2023.2165687
  • Çiftçi, H., Çalişkan, Ç.E., Öztürk, K., Yazici, B. Bioactive nanoparticles synthesized by green method. BSJ Eng Sci, 2021, 4(1): 29-42
  • Gour, A. & Jain N.K. Advances in green synthesis of nanoparticles, Artificial Cells, Nanomedicine, and Biotechnology, (2019), 47:1, 844-851, DOI: 10.1080/21691401.2019.1577878
  • Himalini, S., Uma Maheshwari Nallal, V., Razia, M., Chinnapan, S., ... & Kanimozhi, S. Antimicrobial, anti-melanogenesis and anti-tyrosinase potential of myco-synthesized silver nanoparticles on human skin melanoma SK-MEL-3 cells, Journal of King Saud University - Science, 2022, 34(3), 101882
  • Shukla, A., Iravani, S. Green Synthesis, Characterization and Applications of Nanoparticles; Elsevier: Amsterdam, The Netherlands, 2018.
  • Saini, M., Mushtaq, A., Yadav, S., Rawat, S., Rani, N., Gupta, K. and Saini, K. Green Synthesis of Rod Shaped ZnO using Extract of Origanum majorana Leaf and Investigation for Antibacterial Applications. IOP Conf. Ser.: Mater. Sci. Eng. (2022), 1225 012048
  • Gandhi, P.R., Jayaseelan, C., Mary, R.R., Mathivanan, D., Suseem, S.R. Acaricidal, pediculicidal and larvicidal activity of synthesized ZnO nanoparticles using Momordica charantia leaf extract against blood feeding parasites. Exp Parasitol. (2017) Oct;181:47-56. doi: 10.1016/j.exppara.2017.07.007. Epub 2017 Jul 29. PMID: 28760358.
  • Rajapriya, M., Sharmili, S.A., Baskar, R. et al. Synthesis and Characterization of Zinc Oxide Nanoparticles Using Cynara scolymus Leaves: Enhanced Hemolytic, Antimicrobial, Antiproliferative, and Photocatalytic Activity. J Clust Sci 31, 791–801 (2020).
  • Dinga. E., Mthiyane, D.M.N., Marume, U., Botha, T.L., Horn, S., … Ekennia, A. and Onwudiwe, D.C. Biosynthesis of ZnO nanoparticles using Melia azedarach seed extract: Evaluation of the cytotoxic and antimicrobial potency, OpenNano, (2022), Volume 8, 100068.
  • Dönmez, S. Green synthesis and characterization of zinc oxide nanoparticles by using rhododendron ponticum L leaf extract. Turkish Journal of Health Science and Life (2021), 4(1),54-57
  • Shah, M., Fawcett, D., Sharma, S., Tripathy, S.K., Poinern, G.E.J. Green synthesis of metallic nanoparticles via biological entities. Materials (Basel), (2015), 8(11): 7278–7308.
  • Ansari, A., Ali, A., Khan, N., Umar, M.S., Owais, M. Synthesis of steroidal dihydropyrazole derivatives using green ZnO NPs and evaluation of their anticancer and antioxidant activity. Steroids (2022), 188, 109113.
  • Rehman, H., Ali, W., Khan, N.Z., et al. Delphinium uncinatum mediated biosynthesis of zinc oxide nanoparticles and in-vitro evaluation of their antioxidant, cytotoxic, antimicrobial, anti-diabetic, anti-inflammatory, and anti-aging activities. Saudi J Biol Sci. (2023);30(1):103485.
  • Nagajyothi, P.C., Cha, S.J,, Yang, I.J., Sreekanth, T.V., Kim, K.J., Shin, H.M. Antioxidant and anti-inflammatory activities of zinc oxide nanoparticles synthesized using Polygala tenuifolia root extract. J Photochem Photobiol B. (2015); 146:10-7. doi: 10.1016/j.jphotobiol.2015.02.008.
  • Hemanth Kumar, N.K., Murali, M., Satish, A. et al. Bioactive and Biocompatible Nature of Green Synthesized Zinc Oxide Nanoparticles from Simarouba glauca DC.: An Endemic Plant to Western Ghats, India. J Clust Sci 31, 523–534 (2020). https://doi.org/10.1007/s10876-019-01669-7
  • Dianati, E., Hojati, V., Khayatzadeh, J., Zafar, B.S. The green-synthesized curcumin-mediated zinc oxide nanoparticles (CmZnO-NP) as the exclusive antioxidant and efficient wound healing agent compared with curcumin, methanol, phenytoin, and ZnO. Inorg Nano-Met Chem. (2021). https://doi.org/10.1080/24701556.2021.1956964
Year 2023, Volume: 6 Issue: 3, 150 - 155, 31.12.2023
https://doi.org/10.56150/tjhsl.1391498

Abstract

References

  • Geetha, K., Umadevi, M., Sathe, G. V., & Erenler, R. Spectroscopic investigations on the orientation of 1, 4-dibromonaphthalene on silver nanoparticles. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 116, (2013), 236-241. https://doi: 10.1016/j.saa.2013.07.039
  • Burlec, A. F., Corciova, A., Boev, M., Batir-Marin, D., Mircea, C., Cioanca, O., ... & Hancianu, M. Current Overview of Metal Nanoparticles’ Synthesis, Characterization, and Biomedical Applications, with a Focus on Silver and Gold Nanoparticles. Pharmaceuticals, 16(10), (2023), 1410. https://doi.org/10.3390/ph16101410
  • Sutradhar, P., & Saha, M. Green synthesis of zinc oxide nanoparticles using tomato (Lycopersicon esculentum) extract and its photovoltaic application. Journal of Experimental Nanoscience, 11(5), (2016), 314-327.
  • Erdoğan, Ö., Birtekocak, F., Oryaşın, E., Abbak, M., Demirbolat, G. M., Salih, P. A. Ş. A., & Çevik, Ö. Enginar yaprağı sulu ekstraktı kullanılarak çinko oksit nanopartiküllerinin yeşil sentezi, karakterizasyonu, anti-bakteriyel ve sitotoksik etkileri. Duzce Medical Journal, 21(1), (2019), 19-26.
  • Parveen, K., Banse, V., & Ledwani, L. (2016, April). Green synthesis of nanoparticles: Their advantages and disadvantages. In AIP conference proceedings (Vol. 1724, No. 1). AIP Publishing.
  • Salam, H. A., Sivaraj, R., & Venckatesh, R. Green synthesis and characterization of zinc oxide nanoparticles from Ocimum basilicum L. var. purpurascens Benth.-Lamiaceae leaf extract. Materials letters, 131, (2014), 16-18.
  • Rajendran, S. P., & Sengodan, K. Synthesis and characterization of zinc oxide and iron oxide nanoparticles using Sesbania grandiflora leaf extract as reducing agent. Journal of Nanoscience, 2017.
  • Yuvakkumar, R., Suresh, J., Nathanael, A. J., Sundrarajan, M., & Hong, S. I. Novel green synthetic strategy to prepare ZnO nanocrystals using rambutan (Nephelium lappaceum L.) peel extract and its antibacterial applications. Materials Science and Engineering: C, 41, (2014), 17-27.
  • Agarwal, H., Kumar, S. V., & Rajeshkumar, S. A review on green synthesis of zinc oxide nanoparticles–An eco-friendly approach. Resource-Efficient Technologies, 3(4), (2017), 406-413.
  • 1Zuhrotun, A., Oktaviani, D. J., & Hasanah, A. N. Biosynthesis of Gold and Silver Nanoparticles Using Phytochemical Compounds. Molecules, 28(7), (2023), 3240.
  • Erenler, R., Tugba, A. D. A. K., Karan, T., Elmastas, M., Yildiz, I., Aksit, H., ... & Sanda, M. A. Chemical constituents isolated from Origanum solymicum with antioxidant activitites. The Eurasia Proceedings of Science Technology Engineering and Mathematics, (1), (2017), 139-145.
  • Tepe, B., Cakir, A., & Sihoglu Tepe, A. Medicinal uses, phytochemistry, and pharmacology of Origanum onites (L.): A Review. Chemistry & Biodiversity, 13(5), (2016), 504-520.
  • Erenler, R., Meral, B., Sen, O., Elmastas, M., Aydin, A., Eminagaoglu, O., & Topcu, G. Bioassay-guided isolation, identification of compounds from Origanum rotundifolium and investigation of their antiproliferative and antioxidant activities. Pharmaceutical Biology, 55(1), (2017), 1646-1653.
  • Erenler, R., Demirtas, I., Karan, T., Gul, F., Kayir, O., & Karakoc, O. C. Chemical constituents, quantitative analysis and insecticidal activities of plant extract and essential oil from Origanum onites L. Trends in Phytochemical Research, 2(2), (2018), 91-96.
  • Ozkan, G., Baydar, H., & Erbas, S. The influence of harvest time on essential oil composition, phenolic constituents and antioxidant properties of Turkish oregano (Origanum onites L.). Journal of the Science of Food and Agriculture, 90(2), (2010), 205-209.
  • Pehlivanoglu, S., Acar, C. A., & Donmez, S. Characterization of green synthesized flaxseed zinc oxide nanoparticles and their cytotoxic, apoptotic and antimigratory activities on aggressive human cancer cells. Inorganic and Nano-Metal Chemistry, (2021), 1-10.
  • Aydin Acar, C., Gencer, M. A., Pehlivanoglu, S., Yesilot, S., & Donmez, S. Green and eco‐friendly biosynthesis of zinc oxide nanoparticles using Calendula officinalis flower extract: Wound healing potential and antioxidant activity. International Wound Journal, (2023) https://doi.org/10.1111/iwj.14413.
  • Donmez, S. & Keyvan, E. Green synthesis of zinc oxide nanoparticles using grape seed extract and evaluation of their antibacterial and antioxidant activities, Inorganic and Nano-Metal Chemistry, (2023), DOI: 10.1080/24701556.2023.2165687
  • Çiftçi, H., Çalişkan, Ç.E., Öztürk, K., Yazici, B. Bioactive nanoparticles synthesized by green method. BSJ Eng Sci, 2021, 4(1): 29-42
  • Gour, A. & Jain N.K. Advances in green synthesis of nanoparticles, Artificial Cells, Nanomedicine, and Biotechnology, (2019), 47:1, 844-851, DOI: 10.1080/21691401.2019.1577878
  • Himalini, S., Uma Maheshwari Nallal, V., Razia, M., Chinnapan, S., ... & Kanimozhi, S. Antimicrobial, anti-melanogenesis and anti-tyrosinase potential of myco-synthesized silver nanoparticles on human skin melanoma SK-MEL-3 cells, Journal of King Saud University - Science, 2022, 34(3), 101882
  • Shukla, A., Iravani, S. Green Synthesis, Characterization and Applications of Nanoparticles; Elsevier: Amsterdam, The Netherlands, 2018.
  • Saini, M., Mushtaq, A., Yadav, S., Rawat, S., Rani, N., Gupta, K. and Saini, K. Green Synthesis of Rod Shaped ZnO using Extract of Origanum majorana Leaf and Investigation for Antibacterial Applications. IOP Conf. Ser.: Mater. Sci. Eng. (2022), 1225 012048
  • Gandhi, P.R., Jayaseelan, C., Mary, R.R., Mathivanan, D., Suseem, S.R. Acaricidal, pediculicidal and larvicidal activity of synthesized ZnO nanoparticles using Momordica charantia leaf extract against blood feeding parasites. Exp Parasitol. (2017) Oct;181:47-56. doi: 10.1016/j.exppara.2017.07.007. Epub 2017 Jul 29. PMID: 28760358.
  • Rajapriya, M., Sharmili, S.A., Baskar, R. et al. Synthesis and Characterization of Zinc Oxide Nanoparticles Using Cynara scolymus Leaves: Enhanced Hemolytic, Antimicrobial, Antiproliferative, and Photocatalytic Activity. J Clust Sci 31, 791–801 (2020).
  • Dinga. E., Mthiyane, D.M.N., Marume, U., Botha, T.L., Horn, S., … Ekennia, A. and Onwudiwe, D.C. Biosynthesis of ZnO nanoparticles using Melia azedarach seed extract: Evaluation of the cytotoxic and antimicrobial potency, OpenNano, (2022), Volume 8, 100068.
  • Dönmez, S. Green synthesis and characterization of zinc oxide nanoparticles by using rhododendron ponticum L leaf extract. Turkish Journal of Health Science and Life (2021), 4(1),54-57
  • Shah, M., Fawcett, D., Sharma, S., Tripathy, S.K., Poinern, G.E.J. Green synthesis of metallic nanoparticles via biological entities. Materials (Basel), (2015), 8(11): 7278–7308.
  • Ansari, A., Ali, A., Khan, N., Umar, M.S., Owais, M. Synthesis of steroidal dihydropyrazole derivatives using green ZnO NPs and evaluation of their anticancer and antioxidant activity. Steroids (2022), 188, 109113.
  • Rehman, H., Ali, W., Khan, N.Z., et al. Delphinium uncinatum mediated biosynthesis of zinc oxide nanoparticles and in-vitro evaluation of their antioxidant, cytotoxic, antimicrobial, anti-diabetic, anti-inflammatory, and anti-aging activities. Saudi J Biol Sci. (2023);30(1):103485.
  • Nagajyothi, P.C., Cha, S.J,, Yang, I.J., Sreekanth, T.V., Kim, K.J., Shin, H.M. Antioxidant and anti-inflammatory activities of zinc oxide nanoparticles synthesized using Polygala tenuifolia root extract. J Photochem Photobiol B. (2015); 146:10-7. doi: 10.1016/j.jphotobiol.2015.02.008.
  • Hemanth Kumar, N.K., Murali, M., Satish, A. et al. Bioactive and Biocompatible Nature of Green Synthesized Zinc Oxide Nanoparticles from Simarouba glauca DC.: An Endemic Plant to Western Ghats, India. J Clust Sci 31, 523–534 (2020). https://doi.org/10.1007/s10876-019-01669-7
  • Dianati, E., Hojati, V., Khayatzadeh, J., Zafar, B.S. The green-synthesized curcumin-mediated zinc oxide nanoparticles (CmZnO-NP) as the exclusive antioxidant and efficient wound healing agent compared with curcumin, methanol, phenytoin, and ZnO. Inorg Nano-Met Chem. (2021). https://doi.org/10.1080/24701556.2021.1956964
There are 33 citations in total.

Details

Primary Language English
Subjects Medical Biotechnology (Other)
Journal Section Articles
Authors

Şükriye Yeşilot 0000-0003-3354-8489

Çiğdem Aydın Acar 0000-0002-1311-2314

Publication Date December 31, 2023
Submission Date November 15, 2023
Acceptance Date December 22, 2023
Published in Issue Year 2023 Volume: 6 Issue: 3

Cite

APA Yeşilot, Ş., & Aydın Acar, Ç. (2023). Biosynthesis and In Vitro Antioxidant Activities of Zinc Oxide Nanoparticles with Turkish oregano (Origanum onites L.) Extract. Turkish Journal of Health Science and Life, 6(3), 150-155. https://doi.org/10.56150/tjhsl.1391498