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Antibiofilm activities and in vitro susceptibility testing of eucalyptus (Eucalyptus camaldulensis) essential oil (EO) against fish pathogen Pseudomonas species

Year 2023, Volume: 1 Issue: 1, 8 - 14, 18.06.2023

Serdar Bektaş Murat Özdal Sümeyra Gürkök

Abstract

Essential oils are concoctions of aromatic and volatile chemicals extracted from several plant species. These mixes have been used by society for a variety of reasons, and they play significant functions in nature. This study aimed to analyze the biological properties of essential oil extracted from Eucalyptus camaldulensis leaves, including its antipseudomonal and antibiofilm effects. Antibiotics have been heavily used both to treat bacterial infections and to stimulate fish growth, which has led to the emergence of germs that are resistant to the drugs. The McFarland 0.5 standard was used to determine the study inoculums, and the standard disk diffusion technique was used to analyze the antimicrobial activity. The essential oils from E. camaldulensis possessed antibacterial activity against tested Pseudomonas aeruginosa, P. fluorescens, and P. putida at 5-10 μg/disc. The antibacterial effect was determined to be concentration dependent. Our findings showed that E. camaldulensis essential oil made an excellent source of antipseudomonal, and also exhibited inhibition of Pseudomonas species biofilm formation. Based on its antibacterial and antibiofilm potential, E. camaldulensis essential oil shows promise as a potential source of antibacterial agents. Therefore, the use of E. camaldulensis essential oil in applications may have the potential to be a natural antibacterial agent against pathogenic and spoiling microorganisms.

Keywords

antibacterial activity antibiofilm activity essential oil eucalyptus pseudomonas

References

  • Abebe, G.M. (2020). The role of bacterial biofilm in antibiot ic resistance and food contamination. International journal of microbiology, 2020. https://doi.org/10.1155/2020/1705814
  • Aleksic Sabo, V., & Knezevic, P. (2019). Antimicrobial activity of Eucalyptus camaldulensis Dehn. plant extracts and essential oils: A review. Industrial crops and products, 132, 413-429. https://doi.org/10.1016/j.indcrop.2019.02.051
  • Algammal, A.M., Mabrok, M., Sivaramasamy, E., Youssef, F.M., Atwa, M.H., El-Kholy, A. W., Hetta, H.F., & Hozzein, W.N. (2020). Emerging MDR-Pseudomonas aeruginosa in fish commonly harbor oprL and toxA virulence genes and blaTEM, blaCTX-M, and tetA antibiotic-resistance genes. Scientific reports, 10(1), 15961. https://doi.org/10.1038/s41598-020-72264-4
  • Anastasiou, T.I., Mandalakis, M., Krigas, N., Vézignol, T., Lazari, D., Katharios, P., Dailianis, T., & Antonopoulou, E. (2019). Comparative evaluation of essential oils from medicinal-aromatic plants of Greece: Chemical composition, antioxidant capacity and antimicrobial activity against bacterial fish pathogens. Molecules, 25(1), 148. https://doi.org/10.3390/molecules25010148
  • Andoğan, B.C., Baydar, H., Kaya, S., Demirci, M., Ozbaşar, D., & Mumcu, E. (2002). Antimicrobial activity and chemical composition of some essential oils. Archives of pharmacal research, 25(6), 860-864. https://doi.org/10.1007/BF02977005 Asiaei, E.O., Moghimipour, E., & Fakoor, M.H. (2018). Evaluation of Antimicrobial Activity of Eucalyptus camaldulensis Essential Oil Against the Growth of Drug-Resistant Bacteria. Jundishapur Journal of Natural Pharmaceutical Products, 13(4) :e65050. https://doi.org/10.5812/jjnpp.65050
  • Austin, B., & Austin, D.A. (Editors). (2007). Bacterial fish pathogens. In Bacterial fish pathogens: disease of farmed and wild fish. Springer International Publishing, Cham. pp. 1-19.
  • Barbosa, L.C.A., Filomeno, C.A., & Teixeira, R.R. (2016). Chemical variability and biological activities of Eucalyptus spp. essential oils. Molecules, 21(12), 1671. https://doi.org/10.3390/molecules21121671
  • Bauer, A.W. (1966). Antibiotic susceptibility testing by a standardized single diffusion method. American Journal of Clinical Pathology, 45, 493-496.
  • Bektaş, S., & Özdal, M. (2022). Antimicrobial Activity of Eucalyptus (Eucalyptus camaldulensis) Essential Oil Against Fish Pathogen Bacterium, Aeromonas caviae. Marine Science and Technology Bulletin, 11(4), 467-474. https://doi.org/10.33714/masteb.1184165
  • Čabarkapa, I., Čolović, R., Đuragić, O., Popović, S., Kokić, B., Milanov, D., & Pezo, L. (2019). Anti-biofilm activities of essential oils rich in carvacrol and thymol against Salmonella Enteritidis. Biofouling, 35(3),361–375. https://doi.org/10.1080/08927014.2019.1610169
  • Caputo, L., Smerıglıo, A., Trombetta, D., Cornara, L., Trevena, G., Valussı, M., Fratıannı, F., Feo, Dv., & Nazzaro, F. (2020). Chemical composition and biological activities of the essential oils of Leptospermum petersonii and Eucalyptus gunnii. Frontiers in Microbiology, 11, 1-15. https://doi.org/10.3389/fmicb.2020.00409
  • Cetin, B., Çakmakçı, S., & Çakmakçı, R. (2011). The investigation of antimicrobial activity of thyme and oregano essential oils. Turkish Journal of Agriculture and Forestry, 35(2), 145-154. https://doi.org/10.3906/tar-0906-162 Chen, J., Rossman M.L., & Pawar, D.M. (2007). Attachment of enterohemorragic Escherichia coli to the surface of beef and a culture medium. LWT-Food Science and Technology, 40(2), 249-254. https://doi.org/10.1016/j.lwt.2005.10.011
  • Dincer, S., Masume Uslu, F., & Delik, A. (2020). Antibiotic Resistance in Biofilm. IntechOpen. https://doi.org/10.5772/intechopen.92388
  • Eissa, N., El-Ghiet, E.N., Shaheen, A.A., & Abbass, A.A. (2010). Characterization of pseudomonas species isolated from tilapia "Oreochromis niloticus" in Qaroun and Wadi-El-Rayan lakes, Egypt. Global Veterinaria, 5(2), 116-121
  • Ghalem, B.R., & Mohamed, B. (2008) Antibacterial Activity of Leaf Essential Oils of Eucalyptus globulus and Eucalyptus camaldulensis. African Journal of Pharmacy and Pharmacology, 2(10), 211-215.
  • Goldbeck, J.C., do Nascimento, J.E., Jacob, R.G., Fiorentini, Â.M., & da Silva, W.P. (2014). Bioactivity of essential oils from Eucalyptus globulus and Eucalyptus urograndis against planktonic cells and biofilms of Streptococcus mutans. Industrial Crops and Products, 60, 304-309. https://doi.org/10.1016/j.indcrop.2014.05.030
  • Insuan, W., & Chahomchuen, T. (2020). Chemical composition and antimicrobial activity of essential oil extracted from Eucalyptus citriodora leaf, Microbiology and Biotechnology Letters, 48(2), 148-157. https://doi.org/10.4014/mbl.1912.12016
  • Jorgensen, J.H., & Ferraro, M.J. (2009). Antimicrobial susceptibility testing: a review of general principles and contemporary practices. Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, 49(11), 1749–1755. https://doi.org/10.1086/647952
  • Liu, H., Taylor, T.H., Jr, Pettus, K., Johnson, S., Papp, J.R., & Trees, D. (2016). Comparing the disk-diffusion and agar dilution tests for Neisseria gonorrhoeae antimicrobial susceptibility testing. Antimicrobial resistance and infection control, 5, 46. https://doi.org/10.1186/s13756-016-0148-x
  • Lu, J., Hu, X., & Ren, L. (2022). Biofilm control strategies in food industry: Inhibition and utilization. Trends in Food Science & Technology, 123, 103-113. https://doi.org/10.1016/j.tifs.2022.03.007
  • Mazumder, A., Choudhury, H., Dey, A., & Sarma, D. (2020). Bactericidal Activity of Essential Oil and its Major Compound from Leaves of Eucalyptus maculata Hook. Against Two Fish Pathogens. Journal of Essential Oil Bearing Plants, 23(12), 1-7. https://doi.org/10.1080/0972060X.2020.1729248
  • Merghni, A., Noumi, E., Hadded, O., Dridi, N., Panwar, H., Ceylan, O., Mastouri, M., & Snoussi, M. (2018). Assessment of the antibiofilm and antiquorum sensing activities of Eucalyptus globulus essential oil and its main component 1,8-cineole against methicillin-resistant Staphylococcus aureus strains. Microbial pathogenesis, 118, 74-80. https://doi.org/10.1016/j.micpath.2018.03.006
  • Merghni, A., Marzouki, H., Hentati, H., Aouni, M., & Mastouri, M. (2016). Antibacterial and antibiofilm activities of Laurus nobilis L. essential oil against Staphylococcus aureus strains associated with oral infections. Current Research in Translational Medicine, 64(1), 29-34. https://doi.org/10.1016/j.patbio.2015.10.003
  • Mohamed, S., Nagaraj, G., Chua, F.H.C., & Wang, Y. G. (2000). The use of chemicals in aquaculture in Malaysia and Singapore. In Use of Chemicals in Aquaculture in Asia: Proceedings of the Meeting on the Use of Chemicals in Aquaculture in Asia 20-22 May 1996, Tigbauan, Iloilo, Philippines (pp. 127-140). Aquaculture Department, Southeast Asian Fisheries Development Center.
  • Narvaez, P., Vaughan, D.B., Grutter, A.S., & Hutson, K.S. (2021). New perspectives on the role of cleaning symbiosis in the possible transmission of fish diseases. Reviews in Fish Biology and Fisheries, 31, 233-251. https://doi.org/10.1007/s11160-021-09642-2
  • Nassar, M.S.M., Hazzah, W.A., & Bakr, W.M.K. (2019). Evaluation of antibiotic susceptibility test results: how guilty a laboratory could be?. The Journal of the Egyptian Public Health Association, 94(1), 4. https://doi.org/10.1186/s42506-018-0006-1
  • Oh, W.T., Kim, J.H., Jun, J.W., Giri, S.S., Yun, S., Kim, H.J., Kim, S.G., Kim, S.W., Han, S.J., Kwon, J., & Park, S.C. (2019). Genetic Characterization and Pathological Analysis of a Novel Bacterial Pathogen, Pseudomonas tructae, in Rainbow Trout (Oncorhynchus mykiss). Microorganisms, 7(10), 432. https://doi.org/10.3390/microorganisms7100432
  • Osman, K., Orabi, A., Elbehiry, A., Hanafy, M.H., & Ali, A.M. (2019). Pseudomonas species isolated from camel meat: quorum sensing-dependent virulence, biofilm formation and antibiotic resistance. Future Microbiology, 14(7), 609-622. https://doi.org/10.2217/fmb-2018-0293
  • Park, J.W., Wendt, M., & Heo, G.J. (2016). Antimicrobial activity of essential oil of Eucalyptus globulus against fish pathogenic bacteria. Laboratory animal research, 32(2), 87-90. https://doi.org/10.5625/lar.2016.32.2.87
  • Rodrigues, F.A.F., Silva, G.C.D., Santana, M.F., Bazzolli, D.M.S., Rossi, C.C., & Diaz, M.A.N. (2022). Essential oils isolated from popular medicinal plants and spices as alternative antimicrobial and antibiofilm compounds against the pig pathogen Actinobacillus pleuropneumoniae. Ciência Rural, 53. https://doi.org/10.1590/0103-8478cr20220148
  • Schar, D., Klein, E.Y., Laxminarayan, R., Gilbert, M., & Van Boeckel, T.P. (2020). Global trends in antimicrobial use in aquaculture. Scientific Reports, 10.
  • Sevindik, E., Abacı, Z.T., Yamaner, C., & Ayvaz, M. (2016). Determination of the chemical composition and antimicrobial activity of the essential oils of Teucrium polium and Achillea millefolium grown under North Anatolian ecological conditions. Biotechnology & Biotechnological Equipment, 30(2), 375-380. https://doi.org/10.1080/13102818.2015.1131626
  • Simoes, M., Bennett, R. N., & Rosa, E. A. (2009). Understanding antimicrobial activities of phytochemicals against multidrug resistant bacteria and biofilms. Natural Product Reports, 26(6), 746-757. https://doi.org/10.1039/b821648g
  • Sundell, K., & Wiklund, T. (2011). Effect of biofilm formation on antimicrobial tolerance of Flavobacterium psychrophilum. Journal of fish diseases, 34(5), 373–383. https://doi.org/10.1111/j.1365-2761.2011.01250.x
  • Vestby, L.K., Grønseth, T., Simm, R., & Nesse, L.L. (2020). Bacterial biofilm and its role in the pathogenesis of disease. Antibiotics (Basel, Switzerland), 9(2), 59. https://doi.org/10.3390/antibiotics9020059
  • Wińska, K., Mączka, W., Łyczko, J., Grabarczyk, M., Czubaszek, A., & Szumny, A. (2019). Essential Oils as Antimicrobial Agents-Myth or Real Alternative? Molecules (Basel, Switzerland), 24(11), 2130. https://doi.org/10.3390/molecules24112130
  • Yap, P.S., Yiap, B.C., Ping, H.C., & Lim, S.H. (2014). Essential oils, a new horizon in combating bacterial antibiotic resistance. The open microbiology journal, 8, 6-14. https://doi.org/10.2174/1874285801408010006

Okaliptüs uçucu yağının bazı Pseudomonas türlerine karşı antibiyofilm aktiviteleri ve antimikrobiyal duyarlılığı

Year 2023, Volume: 1 Issue: 1, 8 - 14, 18.06.2023

Serdar Bektaş Murat Özdal Sümeyra Gürkök

Abstract

Uçucu yağlar, çeşitli bitki türlerinden ekstrakte edilen aromatik ve uçucu kimyasalların karışımlarıdır. Bu karışımlar toplum tarafından çeşitli amaçlarla kullanılmakta ve doğada önemli işlevler üstlenmektedirler. Bu çalışma, Eucalyptus camaldulensis yapraklarından ekstrakte edilen esansiyel yağın, antipsödomonal ve antibiyofilm etkileri de dahil olmak üzere biyolojik özelliklerini analiz etmeyi amaçlamıştır. Antibiyotikler hem bakteriyel enfeksiyonları tedavi etmek hem de balık büyümesini teşvik etmek için yoğun bir şekilde kullanılmış ve bu da ilaçlara dirençli mikropların ortaya çıkmasına neden olmuştur. Çalışma inokulumlarını belirlemek için McFarland 0.5 standardı, antimikrobiyal aktiviteyi analiz etmek içinse standart disk difüzyon tekniği kullanıldı. E. camaldulensis'ten elde edilen uçucu yağlar, test edilen Pseudomonas aeruginosa, P. fluorescens ve P. putida'ya karşı 5-10 μg/disk seviyesinde antibakteriyel aktivite gösterdi. Antibakteriyel etkinin konsantrasyona bağlı olduğu belirlendi. Bulgularımız, E. camaldulensis esansiyel yağının mükemmel bir antipsödomonal kaynağı oluşturduğunu ve ayrıca Pseudomonas türlerinin biyofilm oluşumunu inhibe ettiğini gösterdi. Antibakteriyel ve antibiyofilm potansiyeline dayalı olarak, E. camaldulensis esansiyel yağı, potansiyel bir antibakteriyel ajan kaynağı olarak ümit vaat etmektedir. Sonuç olarak, E. camaldulensis uçucu yağının, patojen mikroorganizmalara karşı doğal bir antibakteriyel ajan olma potansiyeline sahip olduğu tespit edilmiştir.

Keywords

antibakteriyel aktivite antibiyofilm aktivite uçucu yağ okaliptüs

References

  • Abebe, G.M. (2020). The role of bacterial biofilm in antibiot ic resistance and food contamination. International journal of microbiology, 2020. https://doi.org/10.1155/2020/1705814
  • Aleksic Sabo, V., & Knezevic, P. (2019). Antimicrobial activity of Eucalyptus camaldulensis Dehn. plant extracts and essential oils: A review. Industrial crops and products, 132, 413-429. https://doi.org/10.1016/j.indcrop.2019.02.051
  • Algammal, A.M., Mabrok, M., Sivaramasamy, E., Youssef, F.M., Atwa, M.H., El-Kholy, A. W., Hetta, H.F., & Hozzein, W.N. (2020). Emerging MDR-Pseudomonas aeruginosa in fish commonly harbor oprL and toxA virulence genes and blaTEM, blaCTX-M, and tetA antibiotic-resistance genes. Scientific reports, 10(1), 15961. https://doi.org/10.1038/s41598-020-72264-4
  • Anastasiou, T.I., Mandalakis, M., Krigas, N., Vézignol, T., Lazari, D., Katharios, P., Dailianis, T., & Antonopoulou, E. (2019). Comparative evaluation of essential oils from medicinal-aromatic plants of Greece: Chemical composition, antioxidant capacity and antimicrobial activity against bacterial fish pathogens. Molecules, 25(1), 148. https://doi.org/10.3390/molecules25010148
  • Andoğan, B.C., Baydar, H., Kaya, S., Demirci, M., Ozbaşar, D., & Mumcu, E. (2002). Antimicrobial activity and chemical composition of some essential oils. Archives of pharmacal research, 25(6), 860-864. https://doi.org/10.1007/BF02977005 Asiaei, E.O., Moghimipour, E., & Fakoor, M.H. (2018). Evaluation of Antimicrobial Activity of Eucalyptus camaldulensis Essential Oil Against the Growth of Drug-Resistant Bacteria. Jundishapur Journal of Natural Pharmaceutical Products, 13(4) :e65050. https://doi.org/10.5812/jjnpp.65050
  • Austin, B., & Austin, D.A. (Editors). (2007). Bacterial fish pathogens. In Bacterial fish pathogens: disease of farmed and wild fish. Springer International Publishing, Cham. pp. 1-19.
  • Barbosa, L.C.A., Filomeno, C.A., & Teixeira, R.R. (2016). Chemical variability and biological activities of Eucalyptus spp. essential oils. Molecules, 21(12), 1671. https://doi.org/10.3390/molecules21121671
  • Bauer, A.W. (1966). Antibiotic susceptibility testing by a standardized single diffusion method. American Journal of Clinical Pathology, 45, 493-496.
  • Bektaş, S., & Özdal, M. (2022). Antimicrobial Activity of Eucalyptus (Eucalyptus camaldulensis) Essential Oil Against Fish Pathogen Bacterium, Aeromonas caviae. Marine Science and Technology Bulletin, 11(4), 467-474. https://doi.org/10.33714/masteb.1184165
  • Čabarkapa, I., Čolović, R., Đuragić, O., Popović, S., Kokić, B., Milanov, D., & Pezo, L. (2019). Anti-biofilm activities of essential oils rich in carvacrol and thymol against Salmonella Enteritidis. Biofouling, 35(3),361–375. https://doi.org/10.1080/08927014.2019.1610169
  • Caputo, L., Smerıglıo, A., Trombetta, D., Cornara, L., Trevena, G., Valussı, M., Fratıannı, F., Feo, Dv., & Nazzaro, F. (2020). Chemical composition and biological activities of the essential oils of Leptospermum petersonii and Eucalyptus gunnii. Frontiers in Microbiology, 11, 1-15. https://doi.org/10.3389/fmicb.2020.00409
  • Cetin, B., Çakmakçı, S., & Çakmakçı, R. (2011). The investigation of antimicrobial activity of thyme and oregano essential oils. Turkish Journal of Agriculture and Forestry, 35(2), 145-154. https://doi.org/10.3906/tar-0906-162 Chen, J., Rossman M.L., & Pawar, D.M. (2007). Attachment of enterohemorragic Escherichia coli to the surface of beef and a culture medium. LWT-Food Science and Technology, 40(2), 249-254. https://doi.org/10.1016/j.lwt.2005.10.011
  • Dincer, S., Masume Uslu, F., & Delik, A. (2020). Antibiotic Resistance in Biofilm. IntechOpen. https://doi.org/10.5772/intechopen.92388
  • Eissa, N., El-Ghiet, E.N., Shaheen, A.A., & Abbass, A.A. (2010). Characterization of pseudomonas species isolated from tilapia "Oreochromis niloticus" in Qaroun and Wadi-El-Rayan lakes, Egypt. Global Veterinaria, 5(2), 116-121
  • Ghalem, B.R., & Mohamed, B. (2008) Antibacterial Activity of Leaf Essential Oils of Eucalyptus globulus and Eucalyptus camaldulensis. African Journal of Pharmacy and Pharmacology, 2(10), 211-215.
  • Goldbeck, J.C., do Nascimento, J.E., Jacob, R.G., Fiorentini, Â.M., & da Silva, W.P. (2014). Bioactivity of essential oils from Eucalyptus globulus and Eucalyptus urograndis against planktonic cells and biofilms of Streptococcus mutans. Industrial Crops and Products, 60, 304-309. https://doi.org/10.1016/j.indcrop.2014.05.030
  • Insuan, W., & Chahomchuen, T. (2020). Chemical composition and antimicrobial activity of essential oil extracted from Eucalyptus citriodora leaf, Microbiology and Biotechnology Letters, 48(2), 148-157. https://doi.org/10.4014/mbl.1912.12016
  • Jorgensen, J.H., & Ferraro, M.J. (2009). Antimicrobial susceptibility testing: a review of general principles and contemporary practices. Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, 49(11), 1749–1755. https://doi.org/10.1086/647952
  • Liu, H., Taylor, T.H., Jr, Pettus, K., Johnson, S., Papp, J.R., & Trees, D. (2016). Comparing the disk-diffusion and agar dilution tests for Neisseria gonorrhoeae antimicrobial susceptibility testing. Antimicrobial resistance and infection control, 5, 46. https://doi.org/10.1186/s13756-016-0148-x
  • Lu, J., Hu, X., & Ren, L. (2022). Biofilm control strategies in food industry: Inhibition and utilization. Trends in Food Science & Technology, 123, 103-113. https://doi.org/10.1016/j.tifs.2022.03.007
  • Mazumder, A., Choudhury, H., Dey, A., & Sarma, D. (2020). Bactericidal Activity of Essential Oil and its Major Compound from Leaves of Eucalyptus maculata Hook. Against Two Fish Pathogens. Journal of Essential Oil Bearing Plants, 23(12), 1-7. https://doi.org/10.1080/0972060X.2020.1729248
  • Merghni, A., Noumi, E., Hadded, O., Dridi, N., Panwar, H., Ceylan, O., Mastouri, M., & Snoussi, M. (2018). Assessment of the antibiofilm and antiquorum sensing activities of Eucalyptus globulus essential oil and its main component 1,8-cineole against methicillin-resistant Staphylococcus aureus strains. Microbial pathogenesis, 118, 74-80. https://doi.org/10.1016/j.micpath.2018.03.006
  • Merghni, A., Marzouki, H., Hentati, H., Aouni, M., & Mastouri, M. (2016). Antibacterial and antibiofilm activities of Laurus nobilis L. essential oil against Staphylococcus aureus strains associated with oral infections. Current Research in Translational Medicine, 64(1), 29-34. https://doi.org/10.1016/j.patbio.2015.10.003
  • Mohamed, S., Nagaraj, G., Chua, F.H.C., & Wang, Y. G. (2000). The use of chemicals in aquaculture in Malaysia and Singapore. In Use of Chemicals in Aquaculture in Asia: Proceedings of the Meeting on the Use of Chemicals in Aquaculture in Asia 20-22 May 1996, Tigbauan, Iloilo, Philippines (pp. 127-140). Aquaculture Department, Southeast Asian Fisheries Development Center.
  • Narvaez, P., Vaughan, D.B., Grutter, A.S., & Hutson, K.S. (2021). New perspectives on the role of cleaning symbiosis in the possible transmission of fish diseases. Reviews in Fish Biology and Fisheries, 31, 233-251. https://doi.org/10.1007/s11160-021-09642-2
  • Nassar, M.S.M., Hazzah, W.A., & Bakr, W.M.K. (2019). Evaluation of antibiotic susceptibility test results: how guilty a laboratory could be?. The Journal of the Egyptian Public Health Association, 94(1), 4. https://doi.org/10.1186/s42506-018-0006-1
  • Oh, W.T., Kim, J.H., Jun, J.W., Giri, S.S., Yun, S., Kim, H.J., Kim, S.G., Kim, S.W., Han, S.J., Kwon, J., & Park, S.C. (2019). Genetic Characterization and Pathological Analysis of a Novel Bacterial Pathogen, Pseudomonas tructae, in Rainbow Trout (Oncorhynchus mykiss). Microorganisms, 7(10), 432. https://doi.org/10.3390/microorganisms7100432
  • Osman, K., Orabi, A., Elbehiry, A., Hanafy, M.H., & Ali, A.M. (2019). Pseudomonas species isolated from camel meat: quorum sensing-dependent virulence, biofilm formation and antibiotic resistance. Future Microbiology, 14(7), 609-622. https://doi.org/10.2217/fmb-2018-0293
  • Park, J.W., Wendt, M., & Heo, G.J. (2016). Antimicrobial activity of essential oil of Eucalyptus globulus against fish pathogenic bacteria. Laboratory animal research, 32(2), 87-90. https://doi.org/10.5625/lar.2016.32.2.87
  • Rodrigues, F.A.F., Silva, G.C.D., Santana, M.F., Bazzolli, D.M.S., Rossi, C.C., & Diaz, M.A.N. (2022). Essential oils isolated from popular medicinal plants and spices as alternative antimicrobial and antibiofilm compounds against the pig pathogen Actinobacillus pleuropneumoniae. Ciência Rural, 53. https://doi.org/10.1590/0103-8478cr20220148
  • Schar, D., Klein, E.Y., Laxminarayan, R., Gilbert, M., & Van Boeckel, T.P. (2020). Global trends in antimicrobial use in aquaculture. Scientific Reports, 10.
  • Sevindik, E., Abacı, Z.T., Yamaner, C., & Ayvaz, M. (2016). Determination of the chemical composition and antimicrobial activity of the essential oils of Teucrium polium and Achillea millefolium grown under North Anatolian ecological conditions. Biotechnology & Biotechnological Equipment, 30(2), 375-380. https://doi.org/10.1080/13102818.2015.1131626
  • Simoes, M., Bennett, R. N., & Rosa, E. A. (2009). Understanding antimicrobial activities of phytochemicals against multidrug resistant bacteria and biofilms. Natural Product Reports, 26(6), 746-757. https://doi.org/10.1039/b821648g
  • Sundell, K., & Wiklund, T. (2011). Effect of biofilm formation on antimicrobial tolerance of Flavobacterium psychrophilum. Journal of fish diseases, 34(5), 373–383. https://doi.org/10.1111/j.1365-2761.2011.01250.x
  • Vestby, L.K., Grønseth, T., Simm, R., & Nesse, L.L. (2020). Bacterial biofilm and its role in the pathogenesis of disease. Antibiotics (Basel, Switzerland), 9(2), 59. https://doi.org/10.3390/antibiotics9020059
  • Wińska, K., Mączka, W., Łyczko, J., Grabarczyk, M., Czubaszek, A., & Szumny, A. (2019). Essential Oils as Antimicrobial Agents-Myth or Real Alternative? Molecules (Basel, Switzerland), 24(11), 2130. https://doi.org/10.3390/molecules24112130
  • Yap, P.S., Yiap, B.C., Ping, H.C., & Lim, S.H. (2014). Essential oils, a new horizon in combating bacterial antibiotic resistance. The open microbiology journal, 8, 6-14. https://doi.org/10.2174/1874285801408010006
There are 37 citations in total.

Details

Primary Language English
Subjects Structural Biology, Fish Pests and Diseases, Fisheries Management
Journal Section Research Articles
Authors

Serdar Bektaş 0000-0002-9620-4969

Murat Özdal 0000-0001-8800-1128

Sümeyra Gürkök 0000-0002-2707-4371

Early Pub Date June 13, 2023
Publication Date June 18, 2023
Submission Date May 30, 2023
Published in Issue Year 2023 Volume: 1 Issue: 1

Cite

APA Bektaş, S., Özdal, M., & Gürkök, S. (2023). Antibiofilm activities and in vitro susceptibility testing of eucalyptus (Eucalyptus camaldulensis) essential oil (EO) against fish pathogen Pseudomonas species. The Trout Journal of Atatürk University, 1(1), 8-14.
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The Trout Journal of Atatürk University (Atatürk Üniversitesi Alabalık Dergisi)

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