Abstract
Polyamines play an important role in the fulfillment of physiological functions in humans and animals. They are responsible for cellular development, cell differentiation, regulation of enzyme activities and ion channels, and DNA and protein synthesis control. Putresin is a polyamine formed by the decarboxylation of ornithine in the body. This substance, which is also known to be synthesized by fungi, is the precursor of other polyamines (spermidine, spermine). In foods, putrescine is important for two reasons. The first is that the amount of biogenic amine is accepted as a quality indicator and the other is that it has toxic health effects. In this study; Electrodes modified with TiO2, CoS, TiO2-CoS nanoparticles were prepared and their usability in the determination of putrescine was investigated. Surface morphology and electrochemical behavior of the modified electrodes were investigated by scanning electron microscopy (SEM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The performance factors of the modified electrodes were determined by differential pulse voltammetry (DPV) method and compared with the performance of the unmodified electrode. The linear operating range of the TiO2-CoS / GCE (glassy carbon electrode) prepared is 0,32 µM - 16,66 µM, the detection limit (LOD) is 0,09 µM and the limit of quantitation (LOQ) is 0,27 µM. Reusability and reproducibility were found to be 4.7% and 3.3%, respectively. The usability of the designed electrode for putresin analysis in food samples was investigated.
Keywords
Cos Electrochemistry Metal oxide Nanotechnology Polyamine Putrescine Sensor TiO2 Voltammetry
References
- Akyüz, E., 2011, Ürik asit tayini için yeni bir biyosensör geliştirilmesi, Trakya Üniversitesi Fen Bilimleri Enstitüsü.
- Apetrei, I. ve Apetrei, C., 2016, Application of voltammetric e-tongue for the detection of ammonia and putrescine in beef products, Sensors and Actuators B: Chemical, 234, 371-379.
- Aslan, E., Akin, I. ve Patir, I. H., 2016, Highly active cobalt sulfide/carbon nanotube catalyst for hydrogen evolution at soft interfaces, Chemistry–A European Journal, 22 (15), 5342-5349.
- Büyükuslu, N., 2014, Besinlerin poliamin içerikleri, Clinical and Experimental Health Sciences, 4 (2), 105-110.
- ERDOĞAN, Z. Ö., KOÇER, M. B., SAYIN, S. ve KÜÇÜKKOLBAŞI, S., 2021, Farmasötik Preparatlarda Ondansetron Tayini İçin Elektrokimyasal Sensör Geliştirilmesi, Journal of the Institute of Science and Technology, 11 (4), 2860-2869.
- Henao-Escobar, W., Del Torno-de Roman, L., Domínguez-Renedo, O., Alonso-Lomillo, M. ve Arcos-Martínez, M., 2016, Dual enzymatic biosensor for simultaneous amperometric determination of histamine and putrescine, Food chemistry, 190, 818-823.
- Leonardo, S. ve Campàs, M., 2016, Electrochemical enzyme sensor arrays for the detection of the biogenic amines histamine, putrescine and cadaverine using magnetic beads as immobilisation supports, Microchimica Acta, 183 (6), 1881-1890.
- Luo, X., Morrin, A., Killard, A. J. ve Smyth, M. R., 2006, Application of nanoparticles in electrochemical sensors and biosensors, Electroanalysis: An International Journal Devoted to Fundamental and Practical Aspects of Electroanalysis, 18 (4), 319-326.
- Melgarejo, E., Urdiales, J. L., Sánchez-Jiménez, F. ve Medina, M. Á., 2010, Targeting polyamines and biogenic amines by green tea epigallocatechin-3-gallate, Amino acids, 38 (2), 519-523.
- Park, H., Kim, S., Kim, T., Kim, Y., Joo, S. W. ve Kang, M., 2021, CoS@ TiO2 S-scheme heterojunction photocatalyst for hydrogen production from photoinduced water splitting, Journal of Cleaner Production, 319, 128819.
- Santos, M. S., 1996, Biogenic amines: their importance in foods, International journal of food microbiology, 29 (2-3), 213-231.
- Shanmugam, S., Thandavan, K., Gandhi, S., Sethuraman, S., Rayappan, J. B. B. ve Krishnan, U. M., 2011, Development and evaluation of a highly sensitive rapid response enzymatic nanointerfaced biosensor for detection of putrescine, Analyst, 136 (24), 5234-5240.
- Telsnig, D., Terzic, A., Krenn, T., Kassarnig, V., Kalcher, K. ve Ortner, A., 2012, Development of a voltammetric amine oxidase-modified biosensor for the determination of biogenic amines in food, Int J Electrochem Sci, 7 (8), 6893-6903.
- Tiwari, K., Tudu, B., Bandyopadhyay, R., Chatterjee, A. ve Pramanik, P., 2018, Voltammetric sensor for electrochemical determination of the floral origin of honey based on a zinc oxide nanoparticle modified carbon paste electrode, Journal of Sensors and Sensor Systems, 7 (1), 319-329.
- Wang, H.-E., Cheng, H., Liu, C., Chen, X., Jiang, Q., Lu, Z., Li, Y. Y., Chung, C., Zhang, W. ve Zapien, J. A., 2011, Facile synthesis and electrochemical characterization of porous and dense TiO2 nanospheres for lithium-ion battery applications, Journal of Power Sources, 196 (15), 6394-6399.
- Yeğin, S., Üren, A. ve Bornova, İ., 2008, Gıdalarda biyojen amin oluşumunu etkileyen faktörler, Türkiye, 10, 21-23.
Elektrokimyasal Putresin Sensörü İçin Nanomalzemelere Dayalı Elektrot Materyallerinin Geliştirilmesi
Abstract
Poliaminler insan ve hayvanlarda fizyolojik işlevlerin yerine getirilmesinde önemli rol alırlar. Hücresel gelişim, hücre farklılaşması, enzim faaliyetlerinin ve iyon kanallarının düzenlenmesi, DNA ve protein sentezi kontrolünde görevlidirler. Putresin vücutta ornitinin dekarboksilasyonuyla oluşan bir poliamindir. Mantarlar tarafından da sentezlendiği bilinen bu madde, diğer poliaminlerin (spermidin, spermin) ön maddesidir. Gıdalarda ise putresin iki nedenle önem arz etmektedir. Birincisi, biyojen amin miktarının kalite indikatörü olarak kabul edilir olması diğeri ise sağlığa etkili toksik etkilerinin bulunmasıdır. Bu çalışmada; TiO2, CoS, TiO2-CoS nanopartikülleri ile modifiye edilmiş elektrotlar hazırlandı ve elektrokimyasal olarak putresin tayininde kullanılabilirliği araştırıldı. Hazırlanan modifiye elektrotların yüzey morfolojileri ve elektrokimyasal davranışları taramalı elektron mikroskopu (SEM), dönüşümlü voltametri (CV) ve elektrokimyasal impedans spektroskopisi (EIS) yöntemleri ile incelendi. Modifiye elektrotların performans faktörleri diferansiyel puls voltametrisi (DPV) yöntemiyle belirlendi ve modifiye edilmemiş elektrodun performansı ile karşılaştırıldı. Hazırlanan TiO2-CoS modifiye camsı karbon elektrodun (GCE) doğrusal çalışma aralığı 0,32 µM - 16,66 µM, gözlenebilme sınırı (LOD) 0,09 µM, alt tayin sınırı (LOQ) 0,27 µM, tekrarlanabilirlik ve tekrar üretilebilirlikleri sırasıyla %4,7 ve %3,3 olarak tespit edildi. Tasarlanan elektrodun gıda numunelerinde putresin analizi için kullanılabilirliği araştırıldı.
References
- Akyüz, E., 2011, Ürik asit tayini için yeni bir biyosensör geliştirilmesi, Trakya Üniversitesi Fen Bilimleri Enstitüsü.
- Apetrei, I. ve Apetrei, C., 2016, Application of voltammetric e-tongue for the detection of ammonia and putrescine in beef products, Sensors and Actuators B: Chemical, 234, 371-379.
- Aslan, E., Akin, I. ve Patir, I. H., 2016, Highly active cobalt sulfide/carbon nanotube catalyst for hydrogen evolution at soft interfaces, Chemistry–A European Journal, 22 (15), 5342-5349.
- Büyükuslu, N., 2014, Besinlerin poliamin içerikleri, Clinical and Experimental Health Sciences, 4 (2), 105-110.
- ERDOĞAN, Z. Ö., KOÇER, M. B., SAYIN, S. ve KÜÇÜKKOLBAŞI, S., 2021, Farmasötik Preparatlarda Ondansetron Tayini İçin Elektrokimyasal Sensör Geliştirilmesi, Journal of the Institute of Science and Technology, 11 (4), 2860-2869.
- Henao-Escobar, W., Del Torno-de Roman, L., Domínguez-Renedo, O., Alonso-Lomillo, M. ve Arcos-Martínez, M., 2016, Dual enzymatic biosensor for simultaneous amperometric determination of histamine and putrescine, Food chemistry, 190, 818-823.
- Leonardo, S. ve Campàs, M., 2016, Electrochemical enzyme sensor arrays for the detection of the biogenic amines histamine, putrescine and cadaverine using magnetic beads as immobilisation supports, Microchimica Acta, 183 (6), 1881-1890.
- Luo, X., Morrin, A., Killard, A. J. ve Smyth, M. R., 2006, Application of nanoparticles in electrochemical sensors and biosensors, Electroanalysis: An International Journal Devoted to Fundamental and Practical Aspects of Electroanalysis, 18 (4), 319-326.
- Melgarejo, E., Urdiales, J. L., Sánchez-Jiménez, F. ve Medina, M. Á., 2010, Targeting polyamines and biogenic amines by green tea epigallocatechin-3-gallate, Amino acids, 38 (2), 519-523.
- Park, H., Kim, S., Kim, T., Kim, Y., Joo, S. W. ve Kang, M., 2021, CoS@ TiO2 S-scheme heterojunction photocatalyst for hydrogen production from photoinduced water splitting, Journal of Cleaner Production, 319, 128819.
- Santos, M. S., 1996, Biogenic amines: their importance in foods, International journal of food microbiology, 29 (2-3), 213-231.
- Shanmugam, S., Thandavan, K., Gandhi, S., Sethuraman, S., Rayappan, J. B. B. ve Krishnan, U. M., 2011, Development and evaluation of a highly sensitive rapid response enzymatic nanointerfaced biosensor for detection of putrescine, Analyst, 136 (24), 5234-5240.
- Telsnig, D., Terzic, A., Krenn, T., Kassarnig, V., Kalcher, K. ve Ortner, A., 2012, Development of a voltammetric amine oxidase-modified biosensor for the determination of biogenic amines in food, Int J Electrochem Sci, 7 (8), 6893-6903.
- Tiwari, K., Tudu, B., Bandyopadhyay, R., Chatterjee, A. ve Pramanik, P., 2018, Voltammetric sensor for electrochemical determination of the floral origin of honey based on a zinc oxide nanoparticle modified carbon paste electrode, Journal of Sensors and Sensor Systems, 7 (1), 319-329.
- Wang, H.-E., Cheng, H., Liu, C., Chen, X., Jiang, Q., Lu, Z., Li, Y. Y., Chung, C., Zhang, W. ve Zapien, J. A., 2011, Facile synthesis and electrochemical characterization of porous and dense TiO2 nanospheres for lithium-ion battery applications, Journal of Power Sources, 196 (15), 6394-6399.
- Yeğin, S., Üren, A. ve Bornova, İ., 2008, Gıdalarda biyojen amin oluşumunu etkileyen faktörler, Türkiye, 10, 21-23.
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Environmental Sciences |
| Journal Section | Research Articles |
| Authors | |
| Publication Date | April 25, 2022 |
| Submission Date | March 14, 2022 |
| Published in Issue | Year 2022 Volume: 48 Issue: 1 |
Cite
Journal Owner: On behalf of Selçuk University Faculty of Science, Rector Prof. Dr. Metin AKSOY
Selcuk University Journal of Science Faculty accepts articles in Turkish and English with original results in basic sciences and other applied sciences. The journal may also include compilations containing current innovations.
It was first published in 1981 as "S.Ü. Fen-Edebiyat Fakültesi Dergisi" and was published under this name until 1984 (Number 1-4).
In 1984, its name was changed to "S.Ü. Fen-Edeb. Fak. Fen Dergisi" and it was published under this name as of the 5th issue.
When the Faculty of Letters and Sciences was separated into the Faculty of Science and the Faculty of Letters with the decision of the Council of Ministers numbered 2008/4344 published in the Official Gazette dated 3 December 2008 and numbered 27073, it has been published as "Selcuk University Journal of Science Faculty" since 2009.
It has been scanned in DergiPark since 2016.
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