Abstract
Birçok çalışma oksidatif stresle ilişkili çeşitli
hastalıkların engellenmesinin yanı sıra insan sağlığına bitki sekonder
metabolitlerinden kaynaklanan faydaları göstermiştir. Bu çalışmada Seseli tortuosum’un antioksidan
aktivitesi serbest radikal süpürme (DPPH), indirgeme gücü (FRAP ve CUPRAC),
toplam antioksidan kapasite (β-karoten/linoleik asit ve fosfomolibdat test)
içeren farklı kimyasal metotlar tarafından araştırıldı. Ayrıca, toplam fenolik
içerik Folin-Ciocalteu testi tarafından tespit edildi. BHA, BHT ve Troloks bu
antioksidan testlerde standart olarak kullanıldı. S. tortuosum’un yağ asiti kompozisyonu Gaz Kromatografisi (GC)
tarafından değerlendirildi. Özütte toplam fenolik içerik 36.58 mgGAE/g özüt
olarak tespit edildi. Özüt antioksidan deneylerde konsantrasyona bağlı olarak
antioksidan aktiviteye sahiptir. S.
tortuosum yağında 24 yağ asiti tanımlandı. Yağ asitleri arasında, linoleik
asit C 18:2 ω6 (%34.57) ana bileşendir. Doymamış yağ asitlerinin miktarı doymuş
yağ asitlerinden daha yüksektir. Bu sonuçlardan, S.
tortuosum yeni gıda ve ilaç ürünlerinin tasarlanması için değerli bir kaynak
olarak düşünülebilir.
References
- 1. Halliwell B, J.M.C. G. Free radicals in Biology and Medicine, 3 rd ed. Oxford University Press. 1999;New York, USA:10-121. 2. Aruoma OI. Assessment of potential prooxidant and antioxidant actions. Journal of the American Oil Chemists' Society. 1996;73(12):1617-25. 3. Hou W-C, Lin R-D, Cheng K-T, Hung Y-T, Cho C-H, Chen C-H, et al. Free radical-scavenging activity of Taiwanese native plants. Phytomedicine. 2003;10(2-3):170-5. 4. Fridovich I. Superoxide radical and superoxide dismutases. Annual review of biochemistry. 1995;64(1):97-112. 5. Halliwell B. Free radicals, antioxidants, and human disease: curiosity, cause, or consequence? The lancet. 1994;344(8924):721-4. 6. Barlow S, Schlatter J. Risk assessment of carcinogens in food. Toxicology and applied pharmacology. 2010;243(2):180-90. 7. Vinson JA, Dabbagh YA, Serry MM, Jang J. Plant flavonoids, especially tea flavonols, are powerful antioxidants using an in vitro oxidation model for heart disease. Journal of Agricultural and Food Chemistry. 1995;43(11):2800-2. 8. Gonçalves MJ, Tavares AC, Cavaleiro C, Cruz MT, Lopes MC, Canhoto J, et al. Composition, antifungal activity and cytotoxicity of the essential oils of Seseli tortuosum L. and Seseli montanum subsp. peixotoanum (Samp.) M. Laínz from Portugal. Industrial Crops and Products. 2012;39:204-9. 9. Ilić MD, Jovanović VPS, Mitić VD, Jovanović OP, Mihajilov-Krstev TM, Marković MS, et al. Comparison of chemical composition and biological activities of Seseli rigidum fruit essential oils from Serbia. Open Chemistry. 2015;13(1). 10. Küpeli E, Tosun A, Yesilada E. Anti-inflammatory and antinociceptive activities of Seseli L. species (Apiaceae) growing in Turkey. Journal of ethnopharmacology. 2006;104(3):310-4. 11. Baytop T. Therapy with Plants in Turkey (Past and Present). 2nd ed Nobel Medical Book House, Istanbul. 1999. 12. Baytop T. Türkçe Bitki Adları Sözlügü, Atatürk Kültür, Dil ve Tarih Yüksek Kurumu, TDKY 3578. TTK Basimevi, Ankara. 1994. 13. Slinkard K, Singleton VL. Total phenol analysis: automation and comparison with manual methods. American Journal of Enology and Viticulture. 1977;28(1):49-55. 14. Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Analytical biochemistry. 1999;269(2):337-41. 15. Dapkevicius A, Venskutonis R, van Beek TA, Linssen JP. Antioxidant activity of extracts obtained by different isolation procedures from some aromatic herbs grown in Lithuania. Journal of the Science of Food and Agriculture. 1998;77(1):140-6. 16. Sokmen A, Gulluce M, Akpulat HA, Daferera D, Tepe B, Polissiou M, et al. The in vitro antimicrobial and antioxidant activities of the essential oils and methanol extracts of endemic Thymus spathulifolius. Food control. 2004;15(8):627-34. 17. Sarikurkcu C. Antioxidant activities of solvent extracts from endemic Cyclamen mirabile Hildebr. tubers and leaves. African Journal of Biotechnology. 2011;10(5):831-9. 18. Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical biochemistry. 1996;239(1):70-6. 19. Apak R, Güçlü K, Özyürek M, Esin Karademir S, Erçağ E. The cupric ion reducing antioxidant capacity and polyphenolic content of some herbal teas. International Journal of Food Sciences and Nutrition. 2006;57(5-6):292-304. 20. IUPAC. Standards methods for Analysis of oils, fats and derivatives Paquot, C. (ed.),. 6th edn, Oxford: Pergamon Press, . 1979:59-66. 21. Matejić J, Džamić A, Mihajilov-Krstev T, Ranđelović V, Krivošej Z, Marin P. Total phenolic content, flavonoid concentration, antioxidant and antimicrobial activity of methanol extracts from three Seseli L. taxa. Open Life Sciences. 2012;7(6):1116-22. 22. Jakovljević D, Vasić S, Stanković M, Čomić L, Topuzović M. In vitro biological activity of secondary metabolites from Seseli rigidum Waldst. et Kit.(Apiaceae). Acta biologica hungarica. 2015;66(4):395-405. 23. Abas F, Khatib A, Shaari K, Lajis NH. Chemical characterization and antioxidant activity of three medicinal Apiaceae species. Industrial Crops and Products. 2014;55:238-47. 24. Coruh N, Celep AS, Özgökçe F. Antioxidant properties of Prangos ferulacea (L.) Lindl., Chaerophyllum macropodum Boiss. and Heracleum persicum Desf. from Apiaceae family used as food in Eastern Anatolia and their inhibitory effects on glutathione-S-transferase. Food chemistry. 2007;100(3):1237-42. 25. Pandey MM, Vijayakumar M, Rastogi S, Rawat AK. Phenolic content and antioxidant properties of selected Indian spices of Apiaceae. Journal of herbs, spices & medicinal plants. 2012;18(3):246-56. 26. Zengin G, Ceylan R, Uysal S, Aktumsek A. Biological Activities of Three Extracts from Artedia squamata: A Study on Antioxidant and Enzyme Inhibitory Potential. Current Bioactive Compounds. 2015;11(3):152-5. 27. Zengin G, Bulut G, Mollica A, Haznedaroglu MZ, Dogan A, Aktumsek A. Bioactivities of Achillea phrygia and Bupleurum croceum based on the composition of phenolic compounds: In vitro and in silico approaches. Food and Chemical Toxicology. 2017. 28. Caprioli G, Fiorini D, Maggi F, Marangoni M, Papa F, Vittori S, et al. Ascorbic acid content, fatty acid composition and nutritional value of the neglected vegetable Alexanders (Smyrnium olusatrum L., Apiaceae). Journal of Food Composition and Analysis. 2014;35(1):30-6. 29. Saracoglu HT, Zengin G, Akin M, Aktumsek A. Evaluation of oil content and fatty acid composition of five endemic Bupleurum species growing in the Central Anatolia region of Turkey. Natural product research. 2012;26(13):1188-94. 30. Saraçoğlu HT, Zengin G, Akin M, Aktümsek A. A comparative study on the fatty acid composition of the oils from five Bupleurum species collected from Turkey. Turkish Journal of Biology. 2012;36(5):527-32.
Abstract
Many
studies have shown the benefits deriving from plants secondary metabolites to
human health also for the prevention of several diseases associated to
oxidative stress. In this work, the antioxidant activity of Seseli tortuosum was investigated by
different chemical methods including free radical scavenging (DPPH assay),
reducing power (FRAP and CUPRAC assays), total antioxidant capacity
(β-caroten/linoleic acid and phosphomolybdate assays). Also, total phenolic
content were detected by Folin-Ciocalteu assay. BHA, BHT and Trolox were used
as standards in these antioxidant assays. The fatty acid composition of S. tortuosum was also evaluated by gas
chromatography (GC). Total phenolic content in the extract was detected as
36.58 mgGAE/g extract. The extract possesses antioxidant activity in a
concentration-dependent manner at the antioxidant assays. Twenty-four fatty
acids were identified in the S. tortuosum
oil. Among fatty acids, the main component was linoleic acid (C 18:2 ω6)
(34.57%). The content in unsaturated fatty acids (UFAs) was higher than that of
saturated fatty acids (SFAs). From these results, S. tortuosum could be considered as a valuable source for designing
novel food and pharmaceutical products.
References
- 1. Halliwell B, J.M.C. G. Free radicals in Biology and Medicine, 3 rd ed. Oxford University Press. 1999;New York, USA:10-121. 2. Aruoma OI. Assessment of potential prooxidant and antioxidant actions. Journal of the American Oil Chemists' Society. 1996;73(12):1617-25. 3. Hou W-C, Lin R-D, Cheng K-T, Hung Y-T, Cho C-H, Chen C-H, et al. Free radical-scavenging activity of Taiwanese native plants. Phytomedicine. 2003;10(2-3):170-5. 4. Fridovich I. Superoxide radical and superoxide dismutases. Annual review of biochemistry. 1995;64(1):97-112. 5. Halliwell B. Free radicals, antioxidants, and human disease: curiosity, cause, or consequence? The lancet. 1994;344(8924):721-4. 6. Barlow S, Schlatter J. Risk assessment of carcinogens in food. Toxicology and applied pharmacology. 2010;243(2):180-90. 7. Vinson JA, Dabbagh YA, Serry MM, Jang J. Plant flavonoids, especially tea flavonols, are powerful antioxidants using an in vitro oxidation model for heart disease. Journal of Agricultural and Food Chemistry. 1995;43(11):2800-2. 8. Gonçalves MJ, Tavares AC, Cavaleiro C, Cruz MT, Lopes MC, Canhoto J, et al. Composition, antifungal activity and cytotoxicity of the essential oils of Seseli tortuosum L. and Seseli montanum subsp. peixotoanum (Samp.) M. Laínz from Portugal. Industrial Crops and Products. 2012;39:204-9. 9. Ilić MD, Jovanović VPS, Mitić VD, Jovanović OP, Mihajilov-Krstev TM, Marković MS, et al. Comparison of chemical composition and biological activities of Seseli rigidum fruit essential oils from Serbia. Open Chemistry. 2015;13(1). 10. Küpeli E, Tosun A, Yesilada E. Anti-inflammatory and antinociceptive activities of Seseli L. species (Apiaceae) growing in Turkey. Journal of ethnopharmacology. 2006;104(3):310-4. 11. Baytop T. Therapy with Plants in Turkey (Past and Present). 2nd ed Nobel Medical Book House, Istanbul. 1999. 12. Baytop T. Türkçe Bitki Adları Sözlügü, Atatürk Kültür, Dil ve Tarih Yüksek Kurumu, TDKY 3578. TTK Basimevi, Ankara. 1994. 13. Slinkard K, Singleton VL. Total phenol analysis: automation and comparison with manual methods. American Journal of Enology and Viticulture. 1977;28(1):49-55. 14. Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Analytical biochemistry. 1999;269(2):337-41. 15. Dapkevicius A, Venskutonis R, van Beek TA, Linssen JP. Antioxidant activity of extracts obtained by different isolation procedures from some aromatic herbs grown in Lithuania. Journal of the Science of Food and Agriculture. 1998;77(1):140-6. 16. Sokmen A, Gulluce M, Akpulat HA, Daferera D, Tepe B, Polissiou M, et al. The in vitro antimicrobial and antioxidant activities of the essential oils and methanol extracts of endemic Thymus spathulifolius. Food control. 2004;15(8):627-34. 17. Sarikurkcu C. Antioxidant activities of solvent extracts from endemic Cyclamen mirabile Hildebr. tubers and leaves. African Journal of Biotechnology. 2011;10(5):831-9. 18. Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical biochemistry. 1996;239(1):70-6. 19. Apak R, Güçlü K, Özyürek M, Esin Karademir S, Erçağ E. The cupric ion reducing antioxidant capacity and polyphenolic content of some herbal teas. International Journal of Food Sciences and Nutrition. 2006;57(5-6):292-304. 20. IUPAC. Standards methods for Analysis of oils, fats and derivatives Paquot, C. (ed.),. 6th edn, Oxford: Pergamon Press, . 1979:59-66. 21. Matejić J, Džamić A, Mihajilov-Krstev T, Ranđelović V, Krivošej Z, Marin P. Total phenolic content, flavonoid concentration, antioxidant and antimicrobial activity of methanol extracts from three Seseli L. taxa. Open Life Sciences. 2012;7(6):1116-22. 22. Jakovljević D, Vasić S, Stanković M, Čomić L, Topuzović M. In vitro biological activity of secondary metabolites from Seseli rigidum Waldst. et Kit.(Apiaceae). Acta biologica hungarica. 2015;66(4):395-405. 23. Abas F, Khatib A, Shaari K, Lajis NH. Chemical characterization and antioxidant activity of three medicinal Apiaceae species. Industrial Crops and Products. 2014;55:238-47. 24. Coruh N, Celep AS, Özgökçe F. Antioxidant properties of Prangos ferulacea (L.) Lindl., Chaerophyllum macropodum Boiss. and Heracleum persicum Desf. from Apiaceae family used as food in Eastern Anatolia and their inhibitory effects on glutathione-S-transferase. Food chemistry. 2007;100(3):1237-42. 25. Pandey MM, Vijayakumar M, Rastogi S, Rawat AK. Phenolic content and antioxidant properties of selected Indian spices of Apiaceae. Journal of herbs, spices & medicinal plants. 2012;18(3):246-56. 26. Zengin G, Ceylan R, Uysal S, Aktumsek A. Biological Activities of Three Extracts from Artedia squamata: A Study on Antioxidant and Enzyme Inhibitory Potential. Current Bioactive Compounds. 2015;11(3):152-5. 27. Zengin G, Bulut G, Mollica A, Haznedaroglu MZ, Dogan A, Aktumsek A. Bioactivities of Achillea phrygia and Bupleurum croceum based on the composition of phenolic compounds: In vitro and in silico approaches. Food and Chemical Toxicology. 2017. 28. Caprioli G, Fiorini D, Maggi F, Marangoni M, Papa F, Vittori S, et al. Ascorbic acid content, fatty acid composition and nutritional value of the neglected vegetable Alexanders (Smyrnium olusatrum L., Apiaceae). Journal of Food Composition and Analysis. 2014;35(1):30-6. 29. Saracoglu HT, Zengin G, Akin M, Aktumsek A. Evaluation of oil content and fatty acid composition of five endemic Bupleurum species growing in the Central Anatolia region of Turkey. Natural product research. 2012;26(13):1188-94. 30. Saraçoğlu HT, Zengin G, Akin M, Aktümsek A. A comparative study on the fatty acid composition of the oils from five Bupleurum species collected from Turkey. Turkish Journal of Biology. 2012;36(5):527-32.
Details
| Subjects | Structural Biology |
|---|---|
| Journal Section | Research Articles |
| Authors | |
| Publication Date | October 31, 2017 |
| Submission Date | May 4, 2017 |
| Published in Issue | Year 2017 Volume: 43 Issue: 2 |
Cite
Journal Owner: On behalf of Selçuk University Faculty of Science, Rector Prof. Dr. Metin AKSOY
Selcuk University Journal of Science Faculty accepts articles in Turkish and English with original results in basic sciences and other applied sciences. The journal may also include compilations containing current innovations.
It was first published in 1981 as "S.Ü. Fen-Edebiyat Fakültesi Dergisi" and was published under this name until 1984 (Number 1-4).
In 1984, its name was changed to "S.Ü. Fen-Edeb. Fak. Fen Dergisi" and it was published under this name as of the 5th issue.
When the Faculty of Letters and Sciences was separated into the Faculty of Science and the Faculty of Letters with the decision of the Council of Ministers numbered 2008/4344 published in the Official Gazette dated 3 December 2008 and numbered 27073, it has been published as "Selcuk University Journal of Science Faculty" since 2009.
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