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The effects of high fructose corn syrup on respiratory system

Year 2015, Volume: 22 Issue: 1, 1 - 7, 30.03.2015

Önder Öztürk Ahmet Akkaya Gülsüm Argüz Özlem Özmen Oğuzhan Kavrık Şeyhmus Kaplan

Abstract

Aim: The effects of high fructose corn syrup diet on rat lungs were examined in this study. Material and methods: Twenty-four Wistar Albino female rats were randomly divided into three groups; Group I: corn syrup diet (30% corn sugar solution for 10 weeks orally). Group 2: corn syrup diet and alpha lipoic acid (100 mg/kg, added to diet after four weeks, orally). Group III: control group. The histopathological changes in lungs were evaluated, and malondialdehyde (MDA) and catalase levels were measured in tissue homogenize samples. Among-group comparisons, ANOVA, Kruskal-Wallis, Mann Whitney-U were used; Bonferroni,Tukey and LSD tests were used for post-hoc analysis. Results: The histopathological changes (hyperemia, edema, lymphoid tissue hyperplasia and inflammatory reaction) in lungs were found statistically different between groups (p = 0.004). While the histopathological changes were increased in Group. I, they were decreased in Group II (p = 0.057). Although MDA levels were statistically different between groups (p <0.005), it was not shown between the groups’catalase levels (p> 0.05). Conclusion: It was shown that corn syrup diet caused histopathological changes in rat lungs, and ALA had an ameliorative effect on these lesions. However further studies are needed to show the mechanisms how corn syrup affects the lungs

Keywords

corn syrup fructose lungs rats

References

  • Dane Ş. Doğal Beslenmeye İnsan Eliyle Müdahale Fruktoz Şurubu. Bilim ve Teknik Dergisi- -Şubat-2011;44 (519):54- 7.
  • Samuel VT. Fructose induced lipogenesis: from sugar to fat to insulin resistance. Trends Endoc Metab. 2011;22(2):60- 5.
  • Korkmaz A. Fruktoz; Kronik Hastalıklar İçin Gizli Bir Tehdit. TAF Prev Med Bull 2008; 7(4):343-46.
  • Ross AP, Bartness TJ, Mielke JG, Parent MB. A high fructose diet impairs spatial memory in male rats. Neurobiology of Learning and Memory, 2009;92: 410–16.
  • Cave M, Deaciuc I, Mendez C, Song Z, Joshi-Barve S, Barve S, McClain C. Nonalcoholic fatty liver disease: predisposing factors and the role of nutrition. Journal of Nutritional Biochemistry 2007; 18(3):184-95.
  • Stirpe F, Della Corte E, Bonetti E, Abbondanza A, Abbati A, De Stefano F. Fructose-induced hyperuricaemia. Lancet 1970;19;2(7686):1310-1.
  • Johnson RJ, Segal MS, Sautin Y, Nakagawa T, Feig DI, Kang DH,et al. Potential role of sugar (fructose) in the epidemic of hypertension, obesity and the metabolic syndrome, diabetes, kidney disease, and cardiovascular disease. Am J Clin Nutr 2007; 86(4):899-906.
  • Nyby MD, Abedi K, Smutko V, Eslami P, Tuck ML. Vascular Angiotensin type 1 receptor expression is associated with vascular dysfunction, oxidative stress and inflammation in fructose-fed rats. Hypertens Res 2007; 30(5):451-7.
  • Busserolles J, Gueux E, Rock E, Demigne C, Mazur A, Rayssiguier Y. Oligofructose protects against the hypertriglyceridemic and pro-oxidative effects of a high fructose diet in rats. J Nutr 2004; 133(6):1903-8.
  • Shinozaki K, Ayajiki K, Nishio Y, Sugaya T, Kashiwagi A, Okamura T. Evidence for a causal role of the renin- angiotensin system in vascular dysfunction associated with insülin resistance. Hypertension 2004; 43(2):255-62.
  • Bell RC, Carlson JC, Storr KC, Herbert K, Sivak J. Highfructose feeding of streptozotocin-diabetic rats is associated with increased cataract formation and increased oxidative stress in the kidney. Br J Nutr 2000; 84(4):575- 82.
  • Yasuno R, Wada H. The biosynthetic pathway for lipoic acid is present in plastids and mitochondria in arabidopsis Thaliana. FEBS Lett, 2002; 517(1-3):110-4.
  • Bullock MW, Brockamann JA, Patterson EL, Pierce JV, Macchi ME. Proposed structures for protogen-A and protogen-B.J. Am. Chem Soc, 1954; 76: 1827-28.
  • Ersoy E, Bayşu N. Biyokimya. Ankara Üniversitesi Veteriner Fakültesi Yayınları, Ankara, 1986: 454.
  • Cremer D.R, Rabeler R, Roberts A, Lynch B. Safety evaluation of α-lipoic acid (ALA). Regulatory Toxicology and Pharmacology, 2006; 46(1): 29-41.
  • Drapper HH, Hadley M. Malondialdehyde determination as index of lipid peroxidation. Methods Enzymol 1990; 186: 421–31.
  • Aebi H. Catalase in vitro. Methods Enzymol. 1984;105:121– 26.
  • Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein dye binding. Anal biochem. 1976;72:248-54.
  • Sanchez-Lozada LG, Tapia E, Jimenez A, Bautista P, Cristobal M, Nepomuceno T, et al. Fructose-induced metabolic syndrome is associated with glomerular hypertension and renal microvascular damage in rats. Am J Physiol Renal Physiol 2007; 292(1):F423-9.
  • Guyton AC. Hall JE. Tibbi Fizyoloji çev.ed: Çavuşoğlu H, Yegen BÇ, Aydın Z, İnci Alican İ. İstanbul: Nobel Tıp Kitabevi Ltd 2013; 881-1027.
  • Nseir W, Nassar F, Assy N. Soft Drinks consumption andnonalcoholic fatty liver disease. World J Gastroent.2010; 7;16(21):2579-88.
  • Chuang CC1, Bumrungpert A, Kennedy A, Overman A, West T, Dawson B, McIntosh MK. Grape powder extract attenuates tumor necrosis factor α-mediated inflammation and insulin resistance in primary cultures of human adipocytes. J Nutr Biochem. 2011;22(1):89-94.
  • Özbayer C, Kurt H, Yangı B. TLR4 ve TLR4 Sinyal Yolağındaki Genetik Varyantların İnsülin Direnci ve Diyabet Riski İle İlişkisi. J Clin Anal Med 2014;5(2): 168-72.
  • Thyagarajan B, Jacobs DR Jr, Smith LJ, Kalhan R, Gross MD, Sood A.Serum adiponectin is positively associated with lung function in young adults, independent of obesity: the CARDIA study. Respir Res. 2010; 11: 176.
  • Wood LG, Gibson PG. Adiponectin: the link between obesity and asthma in women?Am J Respir Crit Care Med. 2012 Jul 1;186(1):1-2.
  • Razolli DS, Moraes JC, Morari J, Moura RF, Vinolo MA, Velloso LA. TLR4 expression in bone marrow-derived cells is both necessary and sufficient to produce the insulin resistance phenotype in diet-induced obesity. Endocrinology. 2015;156(1):103-13.
  • Mohammadi A, Gholamhoseinian A, Fallah H. Zataria multiflora increases insulin sensitivity and PPARγ gene expression in high fructose fed insulin resistant rats. Iran J Basic Med Sci. 2014;17(4):263-70.
  • Sivakumar AS, Anuradha CV. Effect of galangin supplementation on oxidative changes and inflammataory chanes in fructose-fed rat liver. Chemico- Biol Int 2011; 193: 141-148.
  • Kangralkar VA, Patil SD, Bandivadekar RM. Oxidative stress and diabetes: A Review. Int J Pharm Appl 2010; 1: 38-45.
  • Bhagya D, Prema L, Rajamohan T. Therapeutic effects of tender coconut water on oxidative stress in fructose fed insulin resistant hypertensive rats. Asian Pac J Trop Med. 2012;5(4):270-6.
  • Gunes A, Ozmen O, Saygın M, Ascı H, Tok L, Tok O, Dıncoglu D. Lens and cornea lesions of rats fed corn syrup and the protective effects of alpha lipoic acid. Cutan Ocul Toxicol. 2015 Feb 2:1-5. [Epub ahead of print]
  • Ganz M, Csak T, Szabo G. High fat diet feeding results in gender specific steatohepatitis and inflammasome activation. World J Gastroenterol. 2014;20(26):8525-34.
  • Hatzitolios A, Iliadis F, Katsiki N, Baltazi M. Is the hypertensive effect of dietary supplements via aldehydes reduction evidence based? A systematic review. Clin Exp Hypertension 2008; 30: 628-39.
  • Grassi D, Necozione S, Lippi C, Croce G, Valeri L, Pasqualetti P, et al. Cocoa reduces blood pressure and insulin resistance and improves endothelium-dependent vasodilation in hypertensives. Hypertension 2005; 46: 398-405.
  • Grassi D, Desideri G, Necozione S, Lippi C, Casale R, Properzi G, et al. Blood pressure is reduced and insulin sensitivity increased in glucose- intolerant hypertensive subjects after 15 days of consuming high polyphenol dark chocolate. J Nutr 2008; 138: 1671-76.
  • Bustamante J, Lodge JK, Marcocci L, Tritscheler HJ, Packer L, Rihn BH. α-Lipoic acid in liver metabolism and disease. Free Radic Biol Med, 1998; 24(6):1023-39.
  • Pande M, Flora SJS. Lead-induced oxidative damage and its response to combined administration of alpha-lipoic acid and succimers in rats. Toxicology 2002; 177(2-3):187-96.
  • Naveed S, Hazel HR, McMillan DC, Talwar D, O’Reilly DSJ. Acute-phase reactants and plasma trace element concentrations in non-small cell lung cancer patients and controls. Nutr Cancer 1997; 28(3):308-12.
  • Liu J, Atamna H, Kuratsune H, Ames B.N. Delaying brain mitochondrial decay and aging with mitochondrial antioxidants and metabolites. Ann NY Acad. Sci, 2002; 959: 133-66.
  • Biewenga GP, Haenen GR, Bast A. The pharmacology of the antioxidant lipoic acid. Gen Pharmacol 1997; 29(3): 315-17.

Yüksek oranda fruktoz içeren mısır şurubunun solunum sistemine etkisi

Year 2015, Volume: 22 Issue: 1, 1 - 7, 30.03.2015

Önder Öztürk Ahmet Akkaya Gülsüm Argüz Özlem Özmen Oğuzhan Kavrık Şeyhmus Kaplan

Abstract

Amaç: Bu çalışmada, yüksek oranda fruktoz içeren mısır şurubu ile beslenen sıçanlarda mısır şurubunun sıçan akciğerleri üzerindeki etkileri incelendi.
Gereçler ve Yöntem: Yirmi dört adet Wistar Albino dişi rat 3 gruba ayrıldı. Grup 1: Mısır şurubu diyeti (10 hafta %30'luk, oral), Grup 2: Mısır şurubu diyeti +Alfa Lipoik Asit (ALA) (100 mg/kg, 4 haftadan sonra, oral) Grup 3: Kontrol grubu. Deney sonrasında sıçan akciğerlerindeki değişiklikler histopatolojik olarak değerlendirildi. Alınan kan örneklerinde Malondialdehit (MDA) ve Katalaz düzeyleri ölçüldü. Üç grupta elde edilen veriler; Kruskal Wallis, Mann Whitney-U ve ANAVO post hocBonferroni testleri kullanılarak karşılaştırıldı.
Bulgular: Akciğer dokusunun histopatolojik (hiperemi, ödem, lenfoid doku hiperplazisi ve inflamatuvar reaksiyon) değerlendirilmesinde gruplar arasında istatistiksel olarak anlamlı fark mevcuttu (p=0.004). Histopatolojik değişiklikler özellikle Grup I'de artarken, değişikliklerin II. Grupta azaldığı tespit edildi. Grup I ile II arasında istatistiksel olarak anlamlı fark saptanmadı (p=0.057). Kan MDA düzeyleri gruplar arasında farklı olmasına (p<0,005) rağmen, Katalaz düzeylerinde gruplar arasında istatistiksel olarak anlamlı fark bulunmadı (p>0.05).
Sonuç: Mısır şurubu ile beslenen sıçanlarda, mısır şurubu akciğerlerde histopatolojik değişiklere sebep olduğu ve ALA'nın akciğerde oluşan bu lezyonlar üzerinde iyileştirici bir etki gösterdiği saptanmıştır. Fakat mısır şurubunun akciğerleri hangi mekanizmayla etkilediğini gösteren ileri çalışmalara ihtiyaç duyulmaktadır. 

Keywords

mısır şurubu früktoz akciğer sıçan

References

  • Dane Ş. Doğal Beslenmeye İnsan Eliyle Müdahale Fruktoz Şurubu. Bilim ve Teknik Dergisi- -Şubat-2011;44 (519):54- 7.
  • Samuel VT. Fructose induced lipogenesis: from sugar to fat to insulin resistance. Trends Endoc Metab. 2011;22(2):60- 5.
  • Korkmaz A. Fruktoz; Kronik Hastalıklar İçin Gizli Bir Tehdit. TAF Prev Med Bull 2008; 7(4):343-46.
  • Ross AP, Bartness TJ, Mielke JG, Parent MB. A high fructose diet impairs spatial memory in male rats. Neurobiology of Learning and Memory, 2009;92: 410–16.
  • Cave M, Deaciuc I, Mendez C, Song Z, Joshi-Barve S, Barve S, McClain C. Nonalcoholic fatty liver disease: predisposing factors and the role of nutrition. Journal of Nutritional Biochemistry 2007; 18(3):184-95.
  • Stirpe F, Della Corte E, Bonetti E, Abbondanza A, Abbati A, De Stefano F. Fructose-induced hyperuricaemia. Lancet 1970;19;2(7686):1310-1.
  • Johnson RJ, Segal MS, Sautin Y, Nakagawa T, Feig DI, Kang DH,et al. Potential role of sugar (fructose) in the epidemic of hypertension, obesity and the metabolic syndrome, diabetes, kidney disease, and cardiovascular disease. Am J Clin Nutr 2007; 86(4):899-906.
  • Nyby MD, Abedi K, Smutko V, Eslami P, Tuck ML. Vascular Angiotensin type 1 receptor expression is associated with vascular dysfunction, oxidative stress and inflammation in fructose-fed rats. Hypertens Res 2007; 30(5):451-7.
  • Busserolles J, Gueux E, Rock E, Demigne C, Mazur A, Rayssiguier Y. Oligofructose protects against the hypertriglyceridemic and pro-oxidative effects of a high fructose diet in rats. J Nutr 2004; 133(6):1903-8.
  • Shinozaki K, Ayajiki K, Nishio Y, Sugaya T, Kashiwagi A, Okamura T. Evidence for a causal role of the renin- angiotensin system in vascular dysfunction associated with insülin resistance. Hypertension 2004; 43(2):255-62.
  • Bell RC, Carlson JC, Storr KC, Herbert K, Sivak J. Highfructose feeding of streptozotocin-diabetic rats is associated with increased cataract formation and increased oxidative stress in the kidney. Br J Nutr 2000; 84(4):575- 82.
  • Yasuno R, Wada H. The biosynthetic pathway for lipoic acid is present in plastids and mitochondria in arabidopsis Thaliana. FEBS Lett, 2002; 517(1-3):110-4.
  • Bullock MW, Brockamann JA, Patterson EL, Pierce JV, Macchi ME. Proposed structures for protogen-A and protogen-B.J. Am. Chem Soc, 1954; 76: 1827-28.
  • Ersoy E, Bayşu N. Biyokimya. Ankara Üniversitesi Veteriner Fakültesi Yayınları, Ankara, 1986: 454.
  • Cremer D.R, Rabeler R, Roberts A, Lynch B. Safety evaluation of α-lipoic acid (ALA). Regulatory Toxicology and Pharmacology, 2006; 46(1): 29-41.
  • Drapper HH, Hadley M. Malondialdehyde determination as index of lipid peroxidation. Methods Enzymol 1990; 186: 421–31.
  • Aebi H. Catalase in vitro. Methods Enzymol. 1984;105:121– 26.
  • Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein dye binding. Anal biochem. 1976;72:248-54.
  • Sanchez-Lozada LG, Tapia E, Jimenez A, Bautista P, Cristobal M, Nepomuceno T, et al. Fructose-induced metabolic syndrome is associated with glomerular hypertension and renal microvascular damage in rats. Am J Physiol Renal Physiol 2007; 292(1):F423-9.
  • Guyton AC. Hall JE. Tibbi Fizyoloji çev.ed: Çavuşoğlu H, Yegen BÇ, Aydın Z, İnci Alican İ. İstanbul: Nobel Tıp Kitabevi Ltd 2013; 881-1027.
  • Nseir W, Nassar F, Assy N. Soft Drinks consumption andnonalcoholic fatty liver disease. World J Gastroent.2010; 7;16(21):2579-88.
  • Chuang CC1, Bumrungpert A, Kennedy A, Overman A, West T, Dawson B, McIntosh MK. Grape powder extract attenuates tumor necrosis factor α-mediated inflammation and insulin resistance in primary cultures of human adipocytes. J Nutr Biochem. 2011;22(1):89-94.
  • Özbayer C, Kurt H, Yangı B. TLR4 ve TLR4 Sinyal Yolağındaki Genetik Varyantların İnsülin Direnci ve Diyabet Riski İle İlişkisi. J Clin Anal Med 2014;5(2): 168-72.
  • Thyagarajan B, Jacobs DR Jr, Smith LJ, Kalhan R, Gross MD, Sood A.Serum adiponectin is positively associated with lung function in young adults, independent of obesity: the CARDIA study. Respir Res. 2010; 11: 176.
  • Wood LG, Gibson PG. Adiponectin: the link between obesity and asthma in women?Am J Respir Crit Care Med. 2012 Jul 1;186(1):1-2.
  • Razolli DS, Moraes JC, Morari J, Moura RF, Vinolo MA, Velloso LA. TLR4 expression in bone marrow-derived cells is both necessary and sufficient to produce the insulin resistance phenotype in diet-induced obesity. Endocrinology. 2015;156(1):103-13.
  • Mohammadi A, Gholamhoseinian A, Fallah H. Zataria multiflora increases insulin sensitivity and PPARγ gene expression in high fructose fed insulin resistant rats. Iran J Basic Med Sci. 2014;17(4):263-70.
  • Sivakumar AS, Anuradha CV. Effect of galangin supplementation on oxidative changes and inflammataory chanes in fructose-fed rat liver. Chemico- Biol Int 2011; 193: 141-148.
  • Kangralkar VA, Patil SD, Bandivadekar RM. Oxidative stress and diabetes: A Review. Int J Pharm Appl 2010; 1: 38-45.
  • Bhagya D, Prema L, Rajamohan T. Therapeutic effects of tender coconut water on oxidative stress in fructose fed insulin resistant hypertensive rats. Asian Pac J Trop Med. 2012;5(4):270-6.
  • Gunes A, Ozmen O, Saygın M, Ascı H, Tok L, Tok O, Dıncoglu D. Lens and cornea lesions of rats fed corn syrup and the protective effects of alpha lipoic acid. Cutan Ocul Toxicol. 2015 Feb 2:1-5. [Epub ahead of print]
  • Ganz M, Csak T, Szabo G. High fat diet feeding results in gender specific steatohepatitis and inflammasome activation. World J Gastroenterol. 2014;20(26):8525-34.
  • Hatzitolios A, Iliadis F, Katsiki N, Baltazi M. Is the hypertensive effect of dietary supplements via aldehydes reduction evidence based? A systematic review. Clin Exp Hypertension 2008; 30: 628-39.
  • Grassi D, Necozione S, Lippi C, Croce G, Valeri L, Pasqualetti P, et al. Cocoa reduces blood pressure and insulin resistance and improves endothelium-dependent vasodilation in hypertensives. Hypertension 2005; 46: 398-405.
  • Grassi D, Desideri G, Necozione S, Lippi C, Casale R, Properzi G, et al. Blood pressure is reduced and insulin sensitivity increased in glucose- intolerant hypertensive subjects after 15 days of consuming high polyphenol dark chocolate. J Nutr 2008; 138: 1671-76.
  • Bustamante J, Lodge JK, Marcocci L, Tritscheler HJ, Packer L, Rihn BH. α-Lipoic acid in liver metabolism and disease. Free Radic Biol Med, 1998; 24(6):1023-39.
  • Pande M, Flora SJS. Lead-induced oxidative damage and its response to combined administration of alpha-lipoic acid and succimers in rats. Toxicology 2002; 177(2-3):187-96.
  • Naveed S, Hazel HR, McMillan DC, Talwar D, O’Reilly DSJ. Acute-phase reactants and plasma trace element concentrations in non-small cell lung cancer patients and controls. Nutr Cancer 1997; 28(3):308-12.
  • Liu J, Atamna H, Kuratsune H, Ames B.N. Delaying brain mitochondrial decay and aging with mitochondrial antioxidants and metabolites. Ann NY Acad. Sci, 2002; 959: 133-66.
  • Biewenga GP, Haenen GR, Bast A. The pharmacology of the antioxidant lipoic acid. Gen Pharmacol 1997; 29(3): 315-17.
There are 40 citations in total.

Details

Primary Language Turkish
Subjects Dentistry
Journal Section Araştırma Makaleleri
Authors

Önder Öztürk

Ahmet Akkaya

Gülsüm Argüz This is me

Özlem Özmen

Oğuzhan Kavrık This is me

Şeyhmus Kaplan

Publication Date March 30, 2015
Submission Date December 17, 2014
Published in Issue Year 2015 Volume: 22 Issue: 1

Cite

Vancouver Öztürk Ö, Akkaya A, Argüz G, Özmen Ö, Kavrık O, Kaplan Ş. Yüksek oranda fruktoz içeren mısır şurubunun solunum sistemine etkisi. Med J SDU. 2015;22(1):1-7.

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